CHILEAN SOCIETY FOR CELL BIOLOGY. XXVII ANNUAL MEETING October, th, 2013 Puerto Varas

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1 CHILEAN SOCIETY FOR CELL BIOLOGY XXVII ANNUAL MEETING October, th, 2013 Puerto Varas SPONSORS INSTITUTO MILENIO Y ANILLO CENTRO INTERDISCIPLINARIO DE NEUROCIENCIAS DE VALPARAISO CENTRO DE ENVEJECIMIENTO Y REGULACION CELULAR CENTRO FONDAP DE REGULACION DEL GENOMA FUNDACION CHILENA PARA BIOLOGIA CELULAR FUNDACION CIENCIA & VIDA PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE UNIVERSIDAD AUSTRAL DE CHILE EXHIBITORS ALATHEIA MEDICA SA ANDES IMPORT LTDA ARQUIMED SA BD BIOSCIENCES BIOCANT BIOSCHILE IG SA CINDER SERVICES CIENTEC SA FERMELO AS GALENICA SA GENEXPRESS LABTEC LIFE TECHNOLOGIES CHILE LONCOTEC SA TCL LTDA TECNIGEN SA STEMCEL SUDELAB SA

2 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, CHILEAN SOCIETY FOR CELL BIOLOGY XXVII ANNUAL MEETING OCTOBER, 23-27, 2013 PUERTO VARAS P R O G R A M

3 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, WEDNESDAY, OCTOBER 23, :00 13:30 Registration Convention Center Foyer 11:30 12:30 Technical Lecture - Calbuco Room Bio-Rad and Galenica SA Evolución de en la Cuantificación Absoluta de Genes: PCR Digital en Gotas. Fabiana Godoy, Bio-Rad Technical Lecture - Tronador Room BioTek Instruments and BiosChile IGSA Advances in Cell-based Assays for Drug Discovery Peter Banks, PhD, Scientific Director at BioTek Instruments, Inc. USA 12:30 13:30 Technical Lecture - Calbuco Room Tecniplast and Arquimed Modelos Animales en las Investigaciones Biomédicas, Influencia del Manejo y los Factores Ambientales. Juan Martín-Caballero, PhD, Director of Laboratory Animal Units, Parc de Recerca Biomedica de Barcelona-PRBB, Spain. 13:30 15:30 Lunch 15:30 16:30 Inauguration Volcanes Room Chair: Mauricio Gonzalez C. Plenary Lecture Luis Izquierdo Fernandez Chilean Society for Cell Biology Chair: Christian Gonzalez-Billaut Iron and ROS on the Execution Step of Neurodegenerative Diseases. Marco Tulio Núñez. Biology Department, Faculty of Sciences, Universidad de Chile and Research Ring on Oxidative Stress in the Nervous System. 16:30 17:30 Coffee Break Exhibitors Convention Center Foyer 17:30 19:30 Oral Presentations I Volcanes Room Chairs: Mauricio Gonzalez and Maite Castro 17:30 Epigenetic silencing of gene expression in neuronal cells: consequences of the forced expression of Runx2. 1,2 Rodrigo Aguilar, 2 Fernando J. Bustos, 2 Brigitte van Zundert, 1,2 Martin Montecino. 1 FONDAP Center for Genome Regulation and 2 Center for Biomedical Research, Universidad Andres Bello, Santiago, Chile. rod.aguilar@uandresbello.edu 17:45 Wnt3a ligand prevents mitochondrial permeability transition pore opening induced by Aβ oligomers. Macarena S. Arrázola and Nibaldo C. Inestrosa. Centro de Envejecimiento y Regeneración (CARE). Departamento de Biología Celular y Molecular. Facultad de Ciencias Biológicas. Pontificia Universidad Católica de Chile, Santiago, Chile. msarrazo@puc.cl

4 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, :00 Formation of the posterior commissure: a multi-step journey of commissural axons. Stanic K., Gonzalez M., Vera A., Montecinos H., Caprile T. Axon Guidance Laboratory, Department of Cell Biology, University of Concepción, Chile. kstanic@udec.cl 18:15 A failure in energy metabolism and antioxidant uptake precede the onset of Huntington's disease. 1,2, Aníbal I. Acuña, 1,2 Magdalena Esparza, 1,2 Carlos Kramm, 1,2 Felipe A. Beltrán, 1,2 Alejandra Parra, 3 Carlos Cepeda, 4 Carlos Toro, 5 Rene Vidal, 5 Claudio Hetz, 1 Ilona I. Concha, 2,4 Sebastián Brauchi, 3 Michael S. Levine and 1,2 Maite A. Castro. 1 Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile; 2 Centro de Investigación Sur-Austral en Enfermedades del Sistema Nervioso (CISNe), Universidad Austral de Chile; 3 Brain Research Institute, University of California Los Angeles; 4 Instituto de Fisiología, Facultad de Medicina; 5 Instituto de Ciencias Biomédicas, Universidad de Chile, Santiago, Chile. aacuna.sanmartin@alumnos.uach.cl 18:30 trna modification and TOR kinase: a new feedback loop. Rojas-Benítez D, Ibar C and Glavic A. Centro FONDAP de Regulación del Genoma. Facultad de Ciencias, Universidad de Chile. alglavic@uchile.cl 18:45 Identification of an enzyme that crosslinks the 7S dodecamer, an structural domain of collagen IV networks essential for tissue architecture. Carolina Añazco, Lorenzo Vega- Montoto, Mohamed Rafi, Billy G. Hudson and Roberto Vanacore. Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, 37232, USA. carolina.anazco@vanderbilt.edu (Sponsor: M. Castro). 19:00 Decreased expression of RECK, a novel matrix metalloproteinase inhibitor, improves the efficiency of muscle stem cell therapy. Jaime Gutiérrez and Enrique Brandan. Pontificia Universidad Católica de Chile. jgutierrez@bio.puc.cl 19:15 Functional contribution of the transcription factor ATF4 to the pathogenesis of amyotrophic lateral sclerosis (ALS). Vicente Valenzuela 1,2, Melissa Nassif 1,2, Leslie Bargsted 1,2,3, Yerko Fuentealba 1,2 Claudio Hetz 1,2,3 and Soledad Matus 3. Biomedical Neuroscience Institute 1. Institute of Biomedical Sciences. University of Chile. Center for Molecular Studies of the Cell. University of Chile 2. Neurounion Biomedical Foundation, Santiago, Chile 3. soledad.matus@neurounion.com 20:00 Dinner 21:30 22:30 Plenary Lecture Universidad de Antofagasta Volcanes Room Chair: Pedro Zamorano Mechanisms regulating the assembly and integrity of vertebrate synapse. Craig Garner. Stanford University School of Medicine, Department of Psychiatry and Behavioral Sciences, USA. cgarner@stanford.edu

5 THURSDAY, OCTOBER 24, 2013 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, :00 Poster Mounting Session I: N 1 to N 84 Convention Center Foyer 09:00 11:00 Oral Presentations II Volcanes Room Chairs: Alexis Kalergis and Alejandra Alvarez 09:00 Surface expression of Pneumovirus nucleoprotein impairs immunological synapse assembly and function. Pablo F. Céspedes 1, Karen Bohmwald 1, Rodrigo A. Díaz 1, Janyra A. Espinoza 1, Claudia A. Rivera 1, Susan M. Bueno 1, and Alexis M. Kalergis 1,2. 1 Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, and 2 Departamento de Reumatología, Facultad de Medicina, Pontificia Universidad Católica de Chile. pfcesped@bio.puc.cl 09:15 Caveolin-1 is required for LPS-induced dendritic cell maturation. César Oyarce 1,3, Marioly Müller 1, Nicolás Bustamante 1, Felipe Gálvez 1, Francisco Contreras 2, Rodrigo Pacheco 2, Andrew F.G. Quest 3 and Alvaro Lladser 1. 1 Laboratory of Gene Immunotherapy, and 2 Laboratory of Neuroimmunology, Fundación Ciencia & Vida. 3 Laboratory of Cellular Communication, Universidad de Chile. coyarced@gmail.com 09:30 The missing link: mechanisms of action for the effect of complement proteins on human neural stem cells. Francisca Benavente 1,2,6, Katja Piltti 1-4,6, Paul Gershon 5,6, Brian Cummings 1-4,6, and Aileen Anderson 1-4,6. 1 Anatomy & Neurobiology, 2 Sue & Bill Gross Stem Cell Research Center, 3 Physical & Medical Rehabilitation, 4 Institute for Memory Impairments & Neurological Disorders, 5 Molecular Biology and Biochemistry, 6 University of California Irvine, Irvine, CA fbenaven@uci.edu; aja@uci.edu (Sponsor. J.P. Henriquez) 09:45 The unfolded protein response regulates axonal regeneration after sciatic nerve injury. Oñate M. 1,2,3, Hetz C. 1,2,4 and Court F.A. 3,4. 1 Biomedical Neuroscience Institute, 2 Center for Molecular Studies of the Cell, ICBM, Faculty of Medicine, University of Chile. 3 Millennium Nucleus in Regenerative Biology (MINREB), Catholic University of Chile. 4 Neurounion Biomedical Foundation. fcourt@bio.puc.cl, chetz@med.uchile.cl 10:00 Calcium waves in hippocampal neurons and their modulation by endoplasmic reticulum organization. Alex Córdova 1,2,3, Omar Ramírez 1,2,3, Mauricio Cerda 1,2,3, Andrés Couve 1,2, Cecilia Hidalgo 1,2,4, Steffen Härtel 1,2,3. 1 Programs of Anatomy and Developmental Biology and of Physiology and Biophysics, ICBM, 2 Biomedical Neuroscience Institute (BNI), 3 SCIAN-Lab, 4 CEMC, Faculty of Medicine, Universidad de Chile, Santiago, Chile. cordova.alex@gmail.com 10:15 The landscape of RNAs bound to the L1 retrotransposon ORF1p protein in vivo. Jorge Vera-Otarola, Ashfaq Mir, Gaël Cristofari. Institute for Research on Cancer and Aging, Nice (IRCAN), INSERM U1081, CNRS UMR 7284, University of Nice-Sophia-Antipolis, Faculty of Medicine, Nice, France. Jorge.Vera-Otarola@unice.fr (Sponsor: N. Tischler) 10:30 Aβ-production overridden by c-abl inhibition in Alzheimer's transgenic mice and hippocampal neurons. Lisbell Estrada 1,2, David Chamorro 1, Rommy von Bernhardi 3, Nibaldo Inestrosa 2, and Alejandra Alvarez 1,2. 1 Laboratorio de Señalización Celular, 2 CARE-ChileUC, Depto. de Biología Celular y Molecular, 3 Laboratorio de Neurociencias, Escuela de Medicina, Pontificia Universidad Católica de Chile.

6 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, :45 Regulators of phosphatidic acid (PA) levels modulate the specification of sense organs cell types in Drosophila. Patricio Olguín, Jose de la Fuente, Macarena Brito, Amanda Carrasco, Sophie Balmer, Ursula Weber and Marek Mlodzik. Programa de Genética Humana, ICBM, Universidad de Chile, Chile. Mount Sinai School of Medicine, New York, NY, USA. patricioolguin@med.uchile.cl 10:30 13:00 Schools and Science: Students from South of Chile at the Chilean Society for Cell Biology Business Center Room 11:00 13:00 Poster Viewing Session I: 1-84 Odd Numbers Convention Center Foyer Coordinators: Marcela Bravo Claudio Cabello Mario Galindo Carlos Gonzalez (1) Toll-like receptor 2 ligands promote microglial cell death by inducing autophagy. Daniela Arroyo*, Emilia Gaviglio*, Javier Peralta Ramos*, Claudio Bussi*, Constanza Garcia- Keller, Liliana Cancela, Maria Rodriguez-Galan* and Pablo Iribarren*. *CIBICI-CONICET and IFEC-CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina. daniarroyo@fcq.unc.edu.ar (3) Regulation of Na + -K + -ATPase β3-subunit by mineralocorticoids in kidney collecting duct cells. Pablo Díaz, Cristián de Gregorio, Andrea Cutiño, Magdalena González and Luis Michea. Millennium Institute on Immunology and Immunotherapy, CEMC, ICBM, Facultad de Medicina, Universidad de Chile. pdiaz@ciq.uchile.cl (5) Preliminary analysis of the Golgi apparatus proteome in human breast cancer cells. Diego A. Acuña 1, Cecilia Arriagada 1, Breyan H. Ross 1, Viviana Cavieres 1, Patricia V. Burgos 1, Pamela Ehrenfeld 2, and Gonzalo A. Mardones 1, 1 Instituto de Fisiología, and 2 Instituto de Anatomía, Histología y Patología, Facultad de Medicina, Universidad Austral Chile, Valdivia, Chile. diego.acuna.c@gmail.com (7) Role of the ER stress sensor IRE1α in DNA damage responses. Estefanie Dufey 1,2, Hery Urra 1,2, Diego Rodriguez 1,2, Diego Rojas-Rivera 1,2 and Claudio Hetz 1,2,3. 1 Biomedical Neuroscience Institute, 2 Center for Molecular Studies of the Cell, Faculty of medicine, and 3 Neurounion Biomedical Foundation, Santiago, Chile. chetz@med.uchile.cl (9) Detection of prostate specific mrnas in bloodstream for prostate cancer diagnosis. Sánchez, C. 1 ; Fulla, J. 2 ; Campos, D. 1 ; Fleck, D. 1 ; Valdevenito, R. 3 ; García, V 1.; Oyanedel, F 1, Sotelo, D 1.; Ramos, C Urology Department, Clínica Las Condes, 2 Urology Service, Hospital San Borja Arriarán, 3 Urology Service, Hospital Clínico de la Universidad de Chile. csanchezn@clc.cl (Sponsor: Ch. Gonzalez). (11) Avocado oil extracts protect C2C12 myoblasts against acute oxidative stress-induced apoptosis. Marco Araya 1, Arnaldo Gatica 2, and Hugo Olguín 1. 1 Depto. Biología Celular y Molecular, Fac. Ciencias Biológicas, P. Universidad Católica de Chile. 2 INTA, Universidad de Chile. holguin@bio.puc.cl (13) Pyronic, a fret nanosensor for the study of mitochondrial function in intact cells. 1 Sebastián Ceballo, 1,2 Alejandro San Martín, 1 Baeza-Lehnert, F., and 1 L. Felipe Barros. 1 Centro de Estudios Científicos (CECs), Valdivia, 2 Universidad Austral de Chile, Valdivia. sceballo@cecs.cl

7 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, (15) CCR7 expression and its effect on migration of Tumor Antigen Presenting Cells (TAPCells). Ignacio Avalos, Fermin González, Israel Guerrero, Alejandra Gleisner, Flavio Salazar-Onfray. Antitumoral Immunology Laboratory, Faculty of Medicine, University of Chile. kayroz77@gmail.com (17) Harmful CNS inflammation due to respiratory syncytial virus infection. Karen Bohmwald 1, Janyra A. Espinoza 1, Susan M. Bueno 1, Claudia A. Riedel 2 and Alexis M. Kalergis 1,3. 1 Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile. 2 Millenium Institute on Immunology and Immunotherapy, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina Universidad Andrés Bello. 3 Departamento de Reumatología, Facultad de Medicina, Pontificia Universidad Católica de Chile. kbohmwald@uc.cl (19) Characterization of T cell receptor Vβ usage in response to the respiratory syncytial virus. Hernán G. Correa 1, Bruno A. Ramirez 1, Roberto S. Gomez 1,Jorge E. Mora 1 and Alexis M. Kalergis 1,2. 1 Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, 2 Departamento de Reumatología, Facultad de Medicina, Pontificia Universidad Católica de Chile. nanocorrea.ua@gmail.com (21) Design of multi-epitope protein vaccine against B. abortus. Emilia Escalona, Darwin Sáez, Ángel Oñate. Laboratorio de Inmunología Molecular, Universidad de Concepción. emiescalona@udec.cl (23) Impaired learning resulting from RSV infection. Janyra A. Espinoza 1, Karen Bohmwald 1,Claudia M. Cortes 2, Pablo F. Céspedes 1,Roberto S. Gomez 1, Susan M. Bueno 1, Claudia A. Riedel 2 and Alexis M. Kalergis 1,3. 1 Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile. 2 Millennium Institute on Immunology and Immunotherapy, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina Universidad Andrés Bello. 3 Departamento de Reumatología, Facultad de Medicina, Pontificia Universidad Católica de Chile. janyra.espinoza@gmail.com (25) The tumor microenvironment favors the acquisition of a regulatory phenotype by Th17 cells. D. Fernández 1, J.J. Sáez 1, F. Flores 1, M.R. Bono 1, M. Rosemblatt 1,2, D. Sauma 1,2. 1 Departamento de Biología, Facultad de Ciencias, Universidad de Chile. 2 Fundación Ciencia & Vida. dominique.fernandez.q@gmail.com (27) Inactivation of infectious salmon anemia virus (ISAV) for vaccine development. Rivas- Aravena, A 1 ; Fuentes, Y 1 ; Castillo, T 1 ; Imarai, M 2, Spencer, E 1. 1 Laboratorio de Virología, 2 Laboratorio de Inmunología, Centro de Investigación Acuícola, Universidad de Santiago de Chile. (29) In vivo analysis of synaptic toxicity mechanisms of amyloid-beta peptide and alphasynuclein aggregates in Caenorhabditis elegans models of Alzheimer and Parkinson disease. Alicia N Minniti 1, Rebeca Aldunate 1,2. 1 P. Universidad Católica de Chile, 2 Universidad Santo Tomas, Santiago, Chile (31) Characterization of the aggregation of the poly-glutamine domains of the presynaptic proteins Piccolo and Bassoon through canonical yeast assay. Lucía Barra, Pedro Zamorano. Faculty of Health Sciences, University of Antofagasta, Chile. zamorano@gmail.com

8 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, (33) African-Mandenka APP mutant protects from neurodegeneration in Alzheimer s disease model. Bignante EA, Heredia F, Zamponi E, Pinotti JD, Helguera P, Lorenzo A. Instituto de Investigación Médica Mercedes y Martín Ferreyra (INIMEC-CONICET-UNC). Friuli Córdoba-Argentina. (35) Iron chelators induce Dopaminergic neuroregeneration in models of Parkinson Disease. C. Carrasco 1, N.P. Mena 1, P. Aguirre 1, P. Urrutia 1, V. Tapia 1, C. Gonzalez-Billault 1,2 and M.T. Nunez 1,2. 1 Biology, Facultad de Ciencias, Universidad de Chile, 2 Research Ring on Oxidative Stress in the Nervous System, Santiago, Chile. Carloscka@ug.uchile.cl (37) Effect of ERp57 overexpression on the pharmacological model of Parkinson s disease in vivo. Castillo V. 1,2, Valdez P. 1,2, Woehlbier U. 1,2, Mercado G. 1,2, Vidal R. 3 and Hetz C. 1,2,3,4. 1 Biomedical Neuroscience Institute. University of Chile. 2 Center for Molecular Studies of the Cell. University of Chile. 3 Neurounion Biomedical Foundation, Santiago, Chile, 4 Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, USA. vcastillo@ug.uchile.cl (39) Interactions between Schwann cells and peripheral axons during nerve regeneration. Ceci ML, Mardones-Kurslovic C and Allende ML. FONDAP Center for Genome Regulation, Facultad de Ciencias, Universidad de Chile, Santiago, Chile. allende@uchile.cl (41) Time-dependent control of ROCK2 and SYNGAP1 by Wnt5a in hippocampal neurons. Juan F. Codocedo and Nibaldo C. Inestrosa. Centro de Envejecimiento y Regeneración (CARE). Departamento de Biología Celular y Molecular. Facultad de Ciencias Biológicas. P. Universidad Católica de Chile, Santiago, Chile. jfcodoce@uc.cl (43) ROS and Na v channel activity are key in triggering motoneuron death induced by exposure of spinal cord cultures to media conditioned by astrocytes expressing ALScausing mutants in SOD1 and TDP43. Nicole Cortés 1, Fabiola Rojas 1, Sebastián Abarzua 1, Elsa Fritz 1, Brigitte van Zundert 1. 1 Universidad Andrés Bello. nicole.somnus@gmail.com (45) Axonal protein trafficking in a Drosophila model of hereditary spastic paraplegia. Cristian De Gregorio, Jimena Sierralta, Andrés Couve. Program of Physiology and Biophysics, ICBM and Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile. cdegregorio@med.uchile.cl. (47) NMDAR in hippocampus: altered expression in knock-in mice for neuronal surface p antigen. S Espinoza, F Segovia-Miranda, M Bravo-Zehnder, L Massardo, A González. Departamento de Inmunología Clínica y Reumatología, Facultad Medicina. Centro de Envejecimiento y Regeneración (CARE), Departamento de Biología Celular y Molecular, Facultad Ciencias Biológicas. P. Universidad Católica de Chile. csespino@gmail.com (49) Characterization of spinal cord regeneration in zebrafish larvae. Consuelo Anguita, Miguel L. Allende. FONDAP Center for Genome Regulation, Facultad de Ciencias, Universidad de Chile. c.anguitasalinas@gmail.com; allende@uchile.cl (51) Dynamic subcellular localization of Nedd4 may be required for the regulation of myogenic progenitor cell fate. Eduardo de la Vega, Francisco Bustos and Hugo Olguín. Molecular and Cell Biology Department, Faculty of Biological Sciences, P. Universidad Católica de Chile, Santiago, Chile. holguin@bio.puc.cl

9 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, (53) Determination of dopaminergic pathways over mushroom bodies involved in odor discrimination in Drosophila melanogaster. Fuenzalida-Uribe N. and Campusano J.M. Lab of Neurogenetic of Behavior, Dept of Cellular and Molecular Biology, Faculty of Biological Science, Catholic University of Chile. nlfuenzalida.u@gmail.com (55) Effect of spinal cord transplantation in the regenerative capabilities of Xenopus laevis. Emilio Méndez-Olivos, Rosana Muñoz, Mauricio Moreno, Gabriela Edwards, Juan Larraín. Center for Aging and Regeneration, Pontificia Universidad Católica de Chile. eemendez@uc.cl (57) Ric-8A is required for neural crest cells induction. Rodríguez, M., Toro-Tapia, G., Olate, J., Torrejón, M. Laboratorio Genética Molecular Dpto. Bioquímica y Biología Molecular, Universidad de Concepción. marionrodriguez@udec.cl (59) Synthesis of nitric oxide in bipolar cells of dark adapted rat retina. Agurto A 1, Vielma AH 1, Devia V 1,2 and Schmachtenberg O 1. 1 Centro Interdisciplinario de Neurociencia de Valparaíso, Facultad de Ciencias, Universidad de Valparaíso, Chile. 2 Laboratorio de Microscopía Avanzada de Fluorescencia (LAMAF). adolfo.agurto@cinv.cl (61) C/EBPbeta-LAP*-dependent SWI/SNF recruitment in osteoblasts. Cádiz M.I., Meza D., Aguilar R., Sepúlveda H., Montecino M. FONDAP Center for Genome Regulation and Center for Biomedical Research, Andres Bello University, Santiago, Chile. (63) Post-transductional cleavage of C-terminal tail of BMP receptor type II in motor neuron-like cells. Pilar Cárdenas 1, Cristina Pinto 2, Juan Pablo Henríquez 2 and Nelson Osses 1. 1 Institute of Chemistry, P. Catholic University of Valparaiso and 2 Department of Cell Biology and CMA Bio-Bio, University of Concepción, Chile. cheerree@gmail.com (65) The expression of RECK, a novel modulator of extracellular matrix integrity, is regulated by TGF-β: possible clues for proper tissue remodeling. Osvaldo Contreras 1, Chiaki Takahashi 2, Enrique Brandan 1 and Jaime Gutierrez 1. 1 Pontificia Universidad Católica de Chile. 2 Cancer Research Institute, Kanazawa University, Japan. ebrandan@bio.puc.cl; jgutierrez@bio.puc.cl (67) Isolation, characterization, and gene expression of corticosteroid receptors in skeletal muscle of fine flounder (Paralichthys adspersus). Escobar Daniela P., Eduardo N. Fuentes, Diego Safián, Juan Antonio Valdés, Alfredo Molina. Laboratorio de Biotecnología Molecular, Facultad de Ciencias Biologicas, Universidad Andrés Bello, Santiago, Chile. dan.escobarc@gmail.com (69) Angiotensin 1-7 decrease skeletal muscle atrophy induced by lipopolysaccharide. Morales, MG, Simon, F. and Cabello-Verrugio, C. Laboratorio de Biología y Fisiopatología Molecular, Facultad de Ciencias Biológicas, Universidad Andres Bello. claudio.cabello@unab.cl (71) The RUNX2 transcription factor promotes angiogenesis in osteosarcoma via increasing expression of pro-angiogenic genes. Andrés Stevenson 1,2, Sofia Jerez 1,2, Paola Casanello 3, Gary Stein 4, Andre van Wijnen 5, Mario Galindo 1,2. 1 Millennium Institute on Immunology and Immunotherapy, 2 Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile. 3 Instituto de Investigaciones Medicas, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile. 4 Department of Biochemistry, Vermont Cancer Center for Basic and Translational Research, University of Vermont. 5 Departments of Orthopedic Surgery & Biochemistry and Molecular Biology, Mayo Clinic. mgalindo@med.uchile.cl

10 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, (73) Putative mesenchymal stem cells derived from carcinoma-associated stroma of invasive prostate cancer. Cerda-Infante Javier 1, Zumarán Consuelo 1, Conejeros Paola 1, Coronado Romina 1, Alejandro Godoy 2 & Viviana Montecinos 1. 1 Hematology-Oncology & 2 Physiology Departments, P. Universidad Católica de Chile. jcerda.infante@gmail.com (Sponsor: M.A. García). (75) The presence of Coagulation FXa increases tumour volume and metastasis. Ibeth Gonzalez 1, Soledad Lange 1, Rodrigo Valenzuela 1, Kelly Cautivo 1, Alexis M. Kalergis 1,2,5, Veronica Palma 3, Miguel Allende 3, Fabian Tempio 4, Mercedes Lopez 4, Gareth I. Owen 1,5. 1 Facultad de Ciencias Biológicas, 2 Millennium Institute of Immunology and Immunotherapy Pontificia Universidad Católica de Chile. 3 FONDAP Center for Genome Regulation, Facultad de Ciencias. 4 Facultad de Medicina, Universidad de Chile. 5 Biomedical Research Consortium Chile (BMRC). ibalej@gmail.com (77) Reprimo, a p53 dependent G2 arrest mediator, is downregulated in advanced gastric cancer. Saavedra K. 1, 2 ; Olivares W. 1 ; Sandoval A. 1, 2 ; García B. 1 ; Cerda-Infante J. 1 ; Roa JC. 2, 3 ; Corvalan A Department of Hematology and Oncology and Centre for Translational Research in Oncology, School of Medicine, P. Universidad Católica de Chile, Santiago, Chile; 2 Department of Pathology, School of Medicine, Universidad de La Frontera, BIOREN-CEGIN, Temuco, Chile; 3 Pathological Anatomy Department, School of Medicine, P. Universidad Católica de Chile, Santiago, Chile. kattysaavedra@gmail.com (79) Sub-cellular localization of BRCA1 and BARD1 in breast cancer tumors. 1 David Wiener, 1 Cristóbal Herrera, 1 Patricia Gajardo, 1 Elías Blanco, 2 Jorge Gamboa, 2 Valeria Cornejo, 2 Wanda Fernandez, 1 Pilar Carvallo. Department of Cell and Molecular Biology, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile, Unidad de Anatomía Patológica, Hospital San Borja Arriarán, Santiago, Chile. dawiener5@gmail.com (Sponsor: H. Olguín). (81) PERK pathway characterization in labial salivary glands of Sjögren syndrome s patients. V. Bahamondes, S. Aguilera, S. Indo, S. González, C. Molina, C. Leyton, U. Urzúa and MJ González. ICBM-Facultad de Medicina, Universidad de Chile. bacoveronica@gmail.com (83) Vitamin E improves neurological symptoms of Niemann-Pick type C disease mice. Tamara Marín 1, Juan Castro 1, Elisa Balboa 1, Alejandra Alvarez R 2 and Silvana Zanlungo 1. 1 Depto. de Gastroenterología, Facultad de Medicina. 2 Depto. Biología Celular, Pontificia Universidad Católica de Chile. Tamara.marin.m@gmail.com 13:00 14:00 Plenary Lecture Fundación Ciencia & Vida Volcanes Room Chair: Carolina Torrealba 14:00 15:30 Lunch Los Inicios de la Biología Experimental en Chile. Juan de Dios Vial Correa. Pontificia Universidad Católica de Chile. 15:30 17:30 Symposium Novel Aspects of Lysosomes and Autophagy: From Autophagosome Biogenesis to Regulation of Energy Metabolism, Protein Turnover, and Lysosome Reformation Volcanes Room Chairs: Gonzalo Mardones and Francisco Batiz

11 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, A coat that fits all sizes: molecular mechanism of autophagic scaffold assembly and disassembly. Tomas Wollert, Max Planck Institute of Biochemistry, Germany. Lysosomes as novel regulators of nutrient sensing and energy metabolism. Rosa Puertollano, Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. Lysosomes sense early disturbances of the secretory pathway independent of macroautophagy. Patricia Burgos, Laboratory of Cell and Molecular Biology, Department of Physiology, School of Medicine, Universidad Austral de Chile, Chile. Autophagic lysosome reformation. Li Yu, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China. 16:00 19:30 Schools and Science: Students from South of Chile at the Chilean Society for Cell Biology Business Center Room 17:30 19:30 Poster Viewing Session I: 1-84 Even Numbers Convention Center Foyer Coordinators: Marcela Bravo Claudio Cabello Mario Galindo Carlos Gonzalez (2) Role of Panx1 hemichannels in the regulation of the secretory activity of neuroendocrine chromaffin cells. Ximena Baéz 1, María José Olivares 1, María José Guerra 1, Juan C. Saez 1,2, Agustín D. Martínez 1, Ana María Cárdenas 1, Fanny Momboisse 1. 1 Centro Interdisciplinario de Neurociencias de Valparaíso, Universidad de Valparaíso and 2 Pontifícia Universidad Católica de Chile. fanny.momboisse@cinv.cl (4) Molecular and cellular changes of membrane transport machinery in response to a secretory stimulus. García I.A.; Martinez H.; Sampieri L.; and Alvarez, C.I. Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI-CONICET). Facultad de Ciencias Químicas. UNC. Córdoba-Argentina. agarcia@fcq.unc.edu.ar (6) Intracellular trafficking of Sodium-dependent Vitamin C Transporter 2-SVCT2 is modulated by increased extracellular ascorbic acid concentration. 1 Adriana Covarrubias, 1 Eduardo Papic, 2 Patricia Burgos and 1 Maite A. Castro. 1 Instituto de Bioquímica y Microbiología, 2 Instituto de Fisiología, UACh. acovapinto@gmail.com (8) Local trafficking of voltage-gated sodium channels in peripheral axons. Carolina González 1,2, María Javiera Fresno 1,2, José Cánovas 1,2, Eduardo Couve 3, Felipe Court 4 and Andrés Couve 1,2. 1 Physiology and Biophysics, ICBM and 2 Biomedical Neuroscience Institute (BNI), Faculty of Medicine, Universidad de Chile. 3 Faculty of Science, Universidad de Valparaíso. 4 Faculty of Biological Sciences, P. Universidad Católica de Chile. carolags@gmail.com (10) Involvement of ApoER2/Reelin in Schwan cell migration. Consuelo Pasten 1 and María Paz Marzolo 2. Pontificia Universidad Católica de Chile. 1 mpastenr@uc.cl, 2 mmarzolo@bio.puc.cl (12) MLN64 induces mitochondrial dysfunction associated with increased cholesterol transport into mitochondria. Elisa Balboa 1, María-José Pinochet 1, Juan Castro 1, José Fernandez-Checa 2, Silvana Zanlungo 1. 1 Depto. de Gastroenterología, Pontificia Universidad Católica, Santiago, Chile. 2 Centro de Investigaciones Biomédicas Esther Koplowitz, Hospital Clínic i Provincial, Barcelona, España.

12 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, (14) Down-regulation of glucose transport and metabolism by high extracellular glucose concentration. Alejandra Pérez, Marianne Brenet, Carla X. Bittner, Carolina Villanueva, L. Felipe Barros and Carlos B. González. Instituto de Fisiología, Universidad Austral de Chile and Centro de Estudios Científicos. (16) Treatment based on apoptotic bodies induces maturation of C57BL6 mice derived dendritic cells. Barrientos C 1., Diaz X 1., Mena J 1., Lopez X 1., Imarai M 1., Montoya M 1., Acuña-Castillo C 1. Centro de Biotecnología Acuicola y Facultad de Química y Biología, Universidad de Santiago de Chile. claudio.acuna@usach.cl (18) Study of interleukin-2 like factors in Salmo salar. Cifuentes, P., Halçartégaray, N., Wilhelm, V., Rosemblatt, M. Fundación Ciencia & Vida, Universidad Andrés Bello. pablo.cifuentes.palma@gmail.com (20) Reactionary dentin formation accompanies neuro-immune interactions in response to bacterial invasion of dentinal tubules. Couve E (1,2), Osorio R (1), and Schmachtenberg O (2). Departamento de Biología, Laboratorio de Microscopía Electrónica (1) ; Centro Interdisciplinario de Neurociencia de Valparaíso (CINV) (2) ; Facultad de Ciencias, Universidad de Valparaíso, Valparaíso, Chile. oliver.schmachtenberg@uv.cl (22) Development of a model of vaccine against breast cancer. Escrig D. 1, Mena J. 1, Morales J. 1, Barrientos C. 1, Rojas JL 2, Mir S. 3, López X. 1, Acuña-Castillo C. 1 1 Centro de Biotecnología Acuícola y Facultad de Química y Biología, USACH. 2 Facultad de Ciencias Médicas, Universidad de Santiago de Chile. 3 Centro Asistencial Barros Luco. claudio.acuna@usach.cl (24) IFN-α delivers new signals to immunotherapeutic dendritic cell based immunotherapy in vitro. Falcón-Beas C 1, Tempio F 1, Falcón-Beas F 1, Pérez C 1, Flores I 1, Gatica J 1 Pereda C 1, Salazar-Onfray F 1, López MN 1. 1 Millennium Institute on Immunology and Immunotherapy. cristian_falcon_beas@msn.com (26) Stimulation of dopamine receptors D3 and D5 expressed on CD4 + T-cells regulates cellular activation and differentiation by mechanisms involving modulation of ERKsactivation and camp production. Dafne Franz 1, Francisco Contreras 1, Hugo González 1, Daniela Elgueta 1, Carolina Prado 1, Claudio Figueroa 1,2, and Rodrigo Pacheco 1,2. 1 Fundación Ciencia & Vida and 2 Universidad San Sebastián. Santiago, Chile. dafne.franz@gmail.com (28) Hypothyroxinemia during gestation affects the outcome of experimental autoimmune disease (EAE) in the progeny. Henny Haensgen 1, Eduardo Albornoz 1, Susan Bueno 2, Alexis Kalergis 2, Claudia Riedel 1. Universidad Andres Bello. hennyhaensgen@gmail.com (30) Role of the Wnt receptor Frizzled-1 in adult hippocampal neurogenesis. Gabriela A. Andaur 1, Jenny F. Henríquez 1,2, Manuel Varas-Godoy 3, Nibaldo C. Inestrosa 2 and Lorena Varela-Nallar 1. 1 Centro de Investigaciones Biomédicas, Facultad de Ciencias Biológicas y Facultad de Medicina, Universidad Andrés Bello. 2 Centro de Envejecimiento y Regeneración (CARE), Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. 3 Fundación Ciencia y Vida. g.andaur@uandresbello.edu (32) Acute depletion of GSK-3 modulates lysosomal biogenesis leading to a reduction in amyloid precursor protein (APP) levels. Rafael R. Barraza 1,2,3, Viviana Cavieres 3, Patricia V. Burgos 3 and Nibaldo C. Inestrosa 1,2. 1 Centro de Envejecimiento y Regeneración (CARE), 2 Departamento de Biología Celular y Molecular, Pontificia Universidad Católica de Chile, 3 Instituto de Fisiología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile rafarobertbp@gmail.com

13 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, (34) Anti-ribosomal P protein antibodies impair memory once permeating the blood brain barrier. M. Bravo-Zehnder 1,2,3, F. Segovia-Miranda 1,2,3, F. Serrano 3, E. Toledo 3, N.C. Inestrosa 2,3, L. Massardo 1, A. González 1,2,3. 1 Departamento de Inmunología Clínica y Reumatología, Facultad Medicina, Chile. 2 Centro de Envejecimiento y Regeneración, Chile. 3 Facultad Ciencias Biológicas. Pontificia Universidad Católica de Chile. mbravo@med.puc.cl (36) Role of canonical Wnt signaling in age-related neurodegeneration in the CNS of Octodon degu. Francisco J. Carvajal, Juvenal A. Ríos, Cheril Tapia-Rojas, Rocío M. Retamales, Juan A. Godoy and Nibaldo C. Inestrosa. Center for Aging and Regeneration (CARE), Departament of Cell and Molecular Biology, Faculty of Biological Sciences, P. Catholic University of Chile. francisco.carvajalc@hotmail.es (38) Endothelial nitric oxide synthase: synaptic localization and interaction with the neurotrophin receptor TrkB. Ariel Caviedes, Veronica Martinez-Paillama, Francisco Nualart, Ursula Wyneken. Universidad de Concepción y Universidad de los Andes. (40) Identification of new c-abl kinase inhibitors by HTS assays for potential treatment of Alzheimer disease. David Chamorro 1, Lila González 2, Becky King 3, Chris Dextras 3, Xin Hu 3, Marc Ferrer 3, Andres Dulcey 3, Juan Marugan 3, Silvana Zanlungo 2, Alejandra Alvarez R 1. 1 Laboratorio de Señalización Celular, Depto. de Biología Celular, 2 Depto. de Gastroenterología, Escuela de Medicina, Pontificia Universidad Católica de Chile. 3 National Center for Advancing Translational Sciences, National Institutes of Health. ddchamor@uc.cl (42) IRE1 /XBP-1 pathway controls APP metabolism and A aggregation in experimental models of Alzheimer s disease. Víctor Hugo Cornejo 3, Claudia Duran 3, Alexis González 2, Rafael Barraza 2, Andrea Paula-Lima 3, Patricia Burgos 2 and Claudio Hetz 1,3. 1 Harvard School of Public Health, Boston, USA. 2 Universidad Austral, Valdivia. 3 Biomedical Neuroscience Institute, Faculty of Medicine, University of Chile, Santiago, Chile. chetz@med.uchile.cl (44) The fine structure and organization of the membranous axonal organelles. Lillo J. 1, Pihan P. 2,3, González C. 3, Canovas J.A. 3, Couve A. 3 and Court F.A. 2,4. 1 Advanced Microscopy Unit and 2 Millennium Nucleus in Regenerative Biology (MINREB), Catholic University of Chile; 3 Physiology and Biophysics and Biomedical Neuroscience Institute (BNI), ICBM, Faculty of Medicine, Universidad de Chile; 4 Neurounion Biomedical Foundation. fcourt@bio.puc.cl (46) Analysis of brain glucose sensing mechanism using adenoviral-mediated inhibition of MCT1 and MCT4 in tanycytes. Elizondo-Vega R, Carril C, Martinez F, Cortés-Campos C, Nualart F, García-Robles M. Departamento de Biología Celular, Universidad de Concepción. relizondo@udec.cl (48) Characterization of Wnt/ -catenin and BMP/Smad signaling pathways in an in vitro model of amyotrophic lateral sclerosis. Cristina Pinto 1, Pilar Cardenas 2, Nelson Osses 2 Juan Pablo Henríquez 1. Department of Cell Biology and CMA Bio-Bio, Universidad de Concepción 1, Institute of Chemistry, P. Universidad Católica de Valparaiso. jhenriquez@udec.cl (50) Netrin-4 acts as a dependence factor for Neogenin1 in the human neuroblastoma cell line, SH-SY5Y. Arros A., Espinoza N., Vásquez G., Palma V. Laboratory of Stem Cells & Development, Faculty of Sciences, Universidad de Chile, Chile. andrea.arros.v@gmail.com (52) Rising stars in skeletal cell biology: a detailed morphometrical analysis of native osteocytes. Alan Fritz, Patricia Hanna, Ariana Bertin, Francisco Nualart, Sylvain Marcellini. Laboratorio de Desarrollo y Evolución, Universidad de Concepción. alanfritz@udec.cl

14 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, (54) Golgins and mitosis: role of p115 in Drosophila. Ibar, C. and Glavic, A. FONDAP CRG, Facultad de Ciencias, Universidad de Chile. (56) Increased resistance to copper toxicity achieved through previous exposure to the metal in zebrafish. Margarita Parada, Miguel Allende. FONDAP Center for Genome Regulation, Facultad de Ciencias, Universidad de Chile. (58) Transforming growth factor type beta 1 (TGF-β1) produces skeletal muscle atrophy by a mechanism dependent of NADPH oxidase-induced reactive oxygen species. Ábrigo, J., Morales, MG., Simon, F., Cabello-Verrugio, C. Laboratorio de Biología y Fisiopatología Molecular, Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago. claudio.cabello@unab.cl (60) Tumor suppression by Caveolin-1 is linked to inhibition of nitric oxide synthase and HIF-1α-dependent transcription. Sanhueza C., Avalos Y., Silva P., Diaz M.I., Leyton L., Quest A.F.G. Laboratorio de Comunicaciones Celulares, Centro de Estudios Moleculares de la Célula, ICBM, Facultad de Medicina, Universidad de Chile. y.avalos.c@gmail.com (62) Role of the Phosphatidylinositol 3-kinase (PI3K)-AKT pathway in Helicobacter pyloriinduced HIF-1 stabilization and transcriptional activation. Canales J 1, Bravo D 2, Leyton, L 1, Toledo H 3, Quest AFG 1. 1 Laboratorio de Comunicaciones Celulares, Centro de Estudios Moleculares de la Célula (CEMC), 2 Laboratorio de Microbiología Molecular, Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, 3 Laboratorio de Microbiología Oral, Departamento de Patología y Medicina Oral, Facultad de Odontologia, Universidad de Chile. (64) RAP250 is required for normal adipogenesis and TGF-β/SMAD3 signaling pathway transduction. Cataldo LR 1,2,3, Sánchez M 1, Santos JL 2, Cortés V 2, Zorzano A 1. 1 Institute for Research in Biomedicine, Barcelona, Spain. 2 Department of Nutrition, Diabetes and Metabolism, P. Universidad Católica de Chile. 3 PhD program in Biomedical Sciences, Universidad de Los Andes, Chile. rcataldo@ciq.uchile.cl, antonio.zorzano@irbbarcelona.org (66) The death-dependence receptor Neogenin-1 is up-regulated in Hedgehog-associated tumors and mediates Sonic hedgehog-driven precursor proliferation. Falcón P., Casas B., Gallegos I., Cabané P, Gac P., Las Heras F. & Palma V. CTYBD Laboratory, Universidad de Chile. FONDAP Center for Genome Regulation. paulina.falcon.u@gmail.com, barbara.s.casas@gmail.com (68) Wnt/Ca 2+ signaling pathway modulates mitochondrial dynamics in hippocampal neurons. Silva-Alvarez C., Ordenes D, Godoy J, Arrázola M. and Inestrosa N.C. Center for Aging and Regeneration (CARE), Department of Cell and Molecular Biology, Faculty of Biological Sciences, P. Catholic University of Chile, Chile. csilva@bio.puc.cl (70) Ovarian cancer cells induce pro-tumoral phenotype in macrophages in vitro. Abarzua- Catalan, L 1., Kato, S 1., Delpiano, AM 1., Owen, GI 2., Cuello, MA 1. 1 Division of Obstetrics and Gynecology,Faculty of Medicine. 2 Faculty of Biological Sciences, Pontificia Universidad Católica de Chile, Santiago, Chile. lorena.abarzua@gmail.com

15 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, (72) Characterization of Cancer Stem Cells (CSC) in human osteosarcoma cell lines. Carolina Farfan 1,2, Nelson Varela 1,2, Barbara Pesce 1,3, Juan Carlos Aguillón 1,3, Mario Galindo 1,2. 1 Millennium Institute on Immunology and Immunotherapy, 2 Programa de Biología Celular y Molecular, 3 Programa de Inmunología, ICBM, Facultad de Medicina, Universidad de Chile. mgalindo@med.uchile.cl (74) BRCA1 and BARD1 expression and localization in breast cell lines. Cristóbal Herrera, Alonso Quiroz, David Wiener, Elías Blanco, Carolina Álvarez, Pilar Carvallo. Department of Cell and Molecular Biology, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile. ccherrer@uc.cl (Sponsor: K. Gysling). (76) Expression of fructose transporters in prostate cancer. Verónica Torres-Estay 1, Sarah McEvoy 4, Jaime Lizama 1, Pablo Rojas 3, Viviana P. Montecinos 2, Ignacio San Francisco 3, Alejandro S. Godoy 1,4. Departments of Physiology 1, Hematology-Oncology 2, and Urology 3, Pontificia Universidad Católica de Chile, Santiago, Chile; Department of Urology 4, Roswell Park Cancer Institute, Buffalo, NY. vtorres@bio.puc.cl (Sponsor: R. Moreno). (78) Visualization of Moloney murine leukemia virus infection. Ana C. Abbott and Gloria Arriagada. Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Viña del Mar. anaabbottr@gmail.com (80) Role of DCs in Angiotensin II-induced hypertension, inflammation and tissue damage. Daniel Hevia 1,2, Carolina Prado 3, Eugenia Fuentes 1,2, Rodrigo Pacheco 3 y Luis Michea 1,2. 1 Center for Molecular Studies of the Cell; 2 Millennium Institute on Immunology and Immunotherapy, ICBM, Facultad de Medicina, Universidad de Chile; 3 Neuroimmunology Lab, Fundación Ciencia y Vida. (82) Myostatin-induced skeletal muscle atrophy is decreased by Angiotensin 1-7 in skeletal muscle cells. Vázquez, Y., Morales, MG., Cabello-Verrugio, C. Laboratorio de Biología y Fisiopatología Molecular, Facultad de Ciencias Biológicas, Universidad Andres Bello. claudio.cabello@unab.cl (84) Gestational hypothyroidism affects the Blood-Brain-Barrier permeability in the offspring. Luis Venegas* 1, Beatriz Alvarez* 1, Henny Haensgen 1, Susan Bueno 2, Alexis Kalergis 2, Claudia Riedel 1. Universidad Andrés Bello. luis.venegas.s@gmail.com 19:30 20:30 Plenary Lecture Chilean Society for Cell Biology Volcanes Room Chair: Andrés Couve The genetic control of apoptosis and cellular reprogramming in the nematode Caenorhabditis elegans. H. Robert Horvitz. H.H.M.I., Dept. Biology, M.I.T., Cambridge, MA, U.S.A. 20:30 Dinner 22:00 23:30 Best Theses Awards Fundacion Chilena para Biologia Celular Volcanes Room Chairs: Arturo Yudelevich and Mauricio Gonzalez

16 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, Undergraduate Vicente Cataldo von Bohlen Bioquímico P. Universidad Católica de Valparaíso P-53 related protein kinase (PRPK) es necesaria para la formación de lamelipodios y la correcta mantención de la forma celular en hemocitos de Drosophila Thesis Advisor: Alvaro Glavic, Universidad de Chile Graduate Noelia Escobedo Marambio PhD in Biological Sciences P. Universidad Católica de Chile Expression and function of syndecan-4 during mouse development Thesis Advisor: Juan Larraín, P. Universidad Católica de Chile.

17 FRIDAY, OCTOBER 25, 2013 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, :00 Poster Mounting Session II: N 85 to N 168 Convention Center Foyer 09:00 11:00 Oral Presentations III Volcanes Room Chairs: Francisca Bronfman and Juan Larraín 09:00 Targeted epigenetic silencing of PSD95 gene expression with artificial transcription factors. Fernando J Bustos 1,3,4, Lorena Varela-Nallar 3, Rodrigo Aguilar 3,4, Berta Henríquez 3, Marianne Rots 2, Martín Montecino 3,4, Brigitte van Zundert 3. 1 University of Concepcion; 2 UMCG, The Netherlands; 3 Center for Biomedical Research, Andres Bello University; 4 FONDAP C.G.R. fbustosf@gmail.com 09:15 Microtubule-associated protein 1B (MAP1B) role in synapse: Regulation of the structure and functionality of presynaptic and postsynaptic terminals. Bodaleo Felipe 1, Montenegro- Venegas Carolina 1, Court Felipe 2, Pérez Mónica 2, Hartel Steffen 3 and González-Billault Christian 1. (1)Laboratory of Cellular and Neuronal Dynamics, Department of Biology, Faculty of Sciences, Universidad de Chile. (2) Laboratory of Neuroscience and Glial Biology, Departament of Phisiology, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile. (3) Laboratory for Scientific Image Analysis, Faculty of Medicine, Universidad de Chile. chrgonza@uchile.cl 09:30 p75 neurotrophin receptor (p75) evades the endolysosomal route, favoring multivesicular bodies (MVBs) specialized for exosomal release in neuronal cells. Escudero CA 1, Galleguillos C 1, Parraguez JI 1, Lopez-Verrilli MA 1, Cabeza C 1, Saeed U 2, Marzolo MP 1, Carter BD 2, Court FA 1 and Bronfman FC 1. 1 Millennium Nucleus in Regenerative Biology (MINREB), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile. 2 Department of Biochemistry and Vanderbilt Brain Institute. Vanderbilt University Medical School. Tennessee, USA. caescude@gmail.com 09:45 Nitric oxide activates ICAM-1 on the endothelium. Aguilar A 1, Burboa P 1, Sarmiento J 3, Ehrenfeld I 2, Sánchez, FA 1. 1 Immunology Institute, 2 Histology and Pathology Institute, 3 Physiology Institute, Medicine Faculty, UACh. gaynoraguilar24@gmail.com 10:00 EGFR-dependent tumoral growth is sensitive to phosphatidate phosphohydrolase inhibition. Ronan Shaughnessy 1,2, Claudia Oyanadel 1,2, Andrés Norambuena 1,2, Claudio Retamal 1,2, Claudia Metz 1,2, Andrea Soza 1,2 and Alfonso González 1,2. Departamento de Inmunología Clínica y Reumatología, Facultad Medicina 1, Centro de Envejecimiento y Regeneración (CARE), Facultad Ciencias Biológicas 2, Pontificia Universidad Católica de Chile. rpshaughnessy@uc.cl 10:15 Inhibition of the reelin signaling pathway promotes dendritogenesis in mature hippocampal neurons. Estibaliz Ampuero 1, María Paz Marzolo 2 and Brigitte van Zundert 1. 1 Universidad Andrés Bello; 2 P. Universidad Católica de Chile. estiampu@vtr.net 10:30 In vivo study of Sox2+ cells during spinal cord regeneration in Xenopus laevis. Gabriela Edwards-Faret 1,2, Mauricio Moreno 1,2, Hollis Cline 3, Juan Larraín 1,2. 1 Center for Aging and Regeneration, 2 Millenium Nucleus in Regenerative Biology, P. Universidad Católica de Chile, 3 The Scripps Research Institute, La Jolla-United States. gaedwards@uc.cl

18 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, :45 E-cadherin mediated piggybacking drives tissue spreading during epiboly. Germán Reig 1,2, Carolina Figueroa 1,2, Valeria Larenas 1,2, Steffen Härtel 1,2, and Miguel L. Concha 1,2. 1 Laboratory of Experimental Ontogeny and Laboratory for Scientific Image Analysis, ICBM, Faculty of Medicine, University of Chile; 2 Biomedical Neuroscience Institute, Santiago, Chile. mconcha@med.uchile.cl 10:30 13:00 Schools and Science: Students from South of Chile at the Chilean Society for Cell Biology Business Center Room 11:00 13:00 Poster Viewing Session II: Odd Numbers Convention Center Foyer Coordinators: M. Julieta Gonzalez Lisette Leyton Claudia Metz Rodrigo Pacheco (85) Effect of PKC inhibitors on connexin-43 hemichannels. J.L. Vega 1,2,3, A. Vargas 1, C.C. Naus 3, J.C. Sáez 1,4. 1 Departamento de Fisiología, Pontificia Universidad Católica de Chile, Santiago, Chile, 2 Laboratorio de Fisiología Experimental (EPhyL), Universidad de Antofagasta, Antofagasta, Chile, 3 Department of Cellular and Physiological Sciences, Life Science Institute, The University of British Columbia, Vancouver, BC, Canada, 4 Centro Interdisciplinario de Neurociencias de al araíso, al araíso, C ile uvega uc cl (87) Biochemical and cellular characterization of the Golgi apparatus protein GOLPH3 in human glioblastoma multiforme cells. Cecilia Arriagada, Breyan H. Ross, Viviana Cavieres, Patricia V. Burgos, and Gonzalo A. Mardones, Instituto de Fisiología, Facultad de Medicina, Universidad Austral Chile, Valdivia, Chile. carriagada88@gmail.com (89) Characterization of the ER-to-Golgi intermediate compartment (ERGIC) in hippocampal neurons. Juricic M.A. 1,3, Cerda M. 2,3, Cánovas J.A. 1,3, Kukuljan M. 1,3, Härtel S. 2,3, Couve A. 1,3. 1 Physiology and Biophysics, 2 Anatomy and Developmental Biology, 3 Biomedical Neuroscience Institute (BNI), ICBM, Faculty of Medicine, Universidad de Chile. mangelesj@gmail.com (91) Sorting behaviour of the different splice variants of ApoER2 and the role of the adaptor complex AP4. Farfán P. 1, Benítez ML 1, Larios J 1, Cáceres 2 and Marzolo MP 1 1 Lab. Tráfico Intracelular y Señalización, Depto. Biología Celular Molecular, Fac. Ciencias Biológicas P. Universidad Católica de Chile, 2 Instituto Investigación Médica Mercedes y Martin Ferreyra Córdoba, Argentina. mmarzolo@bio.puc.cl (93) Novel association of Nephrin with Pdlim2 in the murine glomerulus. Garmendia Liliana, Acuña Stephanie, Valdés Gloria, Cuello Mauricio and Machuca Eduardo. Centro Investigaciones Médicas, Escuela Medicina, Pontificia Universidad Católica, Santiago, Chile. liliana.garmendia.c@gmail.com (95) Synthesis and evaluation of iron chelators directed to mitochondria of dopaminergic neurons as potential therapeutic agents for Parkinson's disease. 1 Fernanda Lourido, 2 Olimpo García-Beltrán, 1 Marco Tulio Núñez, 1 Natalia P. Mena. 1 Iron and Biology of Aging Laboratory and 2 Biodynamic Chemistry Laboratory, Faculty of Sciences, Universidad de Chile. fernanda.lourido@gmail.com (97) TAPCells based vaccination induce specific anti NY-ESO1 immune response in melanoma patients. Falcon-Beas F 1 ; Tempio F 1 ; Guerrero P 1 ; Flores I; Falcon-Beas C; Gatica J; Salazar-Onfray F 1,2 ; Mercedes L 1,2. (1) Immunology Program. Biomedical Science Institute. Universidad de Chile. (2) Millennium Institute on Immunology and Immunotherapy, Faculty of Medicine, University of Chile, Santiago, Chile.

19 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, (99) NFκB activation during intradermal DNA vaccination controls migration of skinderived dendritic cells and induction of tumor-protective CTL responses. Felipe Gálvez- Cancino, Nicole Rojas-Colonelli, Jonathan Roco, Camila Flores, Paola Murgas, Alvaro Lladser. Laboratory of Gene Immunotherapy, Fundación Ciencia & Vida. (101) B cell-deficient mice infected with Trypanosoma cruzi develop a pro-inflammatory T cell response potentially harmful for the host. Gorosito-Serrán, M., Tosello-Boari, J., Bermejo, D.; Ramello, M.C.; Fiocca-Vernengo, F.; Montes C.; Acosta-Rodríguez, E.; Gruppi, A. CIBICI FCQ, UNC. mgorosito@fcq.unc.edu.ar (103) Respiratory syncytial virus infection induces Heme Oxygenase-1 on dendritic cells and modulates progression of disease. Miguel A. León 1, Susan M. Bueno 1, Claudia A. Riedel 2 and Alexis M. Kalergis 1,3. 1 Millennium Institute of Immunology and Immunotherapy (MIII), Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile. 2 MIII, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina Universidad Andrés Bello. 3 Departamento de Reumatología, Facultad de Medicina. Pontificia Universidad Católica de Chile. Miguel.leon.rios@gmail.com (105) Effect of tumor epithelial and normal stroma mammary cells on CD4 + T lymphocyte differentiation. C. Miller 1,3,, N. Tobar 3, M.R. Bono 1, M.Rosemblatt 2, J. Martínez 3. 1 Facultad de Ciencias, Universidad de Chile. 2 Fundación Ciencia para la Vida. 3 INTA, Universidad de Chile. cmillerv@gmail.cl (107) Aldosterone modulates the surface expression of TLR-4/M2D in dendritic cells enhancing their response to LPS in vivo. Natalia Muñoz-Durango 1 and Alexis M. Kalergis 1,2. 1 Millennium Institute on Immunology and Immunotherapy, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile. 2 Departamento de Reumatología, Facultad de Medicina, Pontificia Universidad Católica de Chile. nmunoz4@uc.cl (109) Regulation of cytokine expression in atlantic salmon is related to oxidative stress. Rodríguez FE., Valenzuela B., Imarai M. Laboratorio de Inmunología, Centro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile. felipe.rt@gmail.com (111) Regulation of PSD-95 palmitoylation and APT-1 expression by Wnt signaling pathways in hippocampal neurons. Juan F. Codocedo, Ting Cao and Nibaldo C. Inestrosa. Centro de Envejecimiento y Regeneración (CARE). Departamento de Biología Celular y Molecular. Facultad de Ciencias Biológicas. P. Universidad Católica de Chile, Santiago, Chile. jfcodoce@uc.cl (113) The transition of the BDNF receptor TrkB from Rab5 to Rab11 endosomes is required for BDNF-induced ramification and activation of CREB transcription factor in hippocampal neurons. Andrés González, Iván Pinto, Oscar Lazo and Francisca Bronfman. Neuronal Cell Biology and Regeneration Lab, Pontificia Universidad Católica de Chile. augonzal@uc.cl (115) Necroptosis triggers mitochondrial failure and neurite degeneration in hippocampal neurons subjected to excitotoxicity. Hernández D.E. 1 and Court, F.A. 1,2. 1 Millennium Nucleus in Regenerative Biology, (MINREB), P. Catholic University of Chile and 2 Neurounion Biomedical Foundation. dehernan@uc.cl; fcourt@bio.puc.cl

20 Chilean Society for Cell Biology XXVII Annual Meeting October 23-27, (117) CoREST downregulates Notch pathway during cerebral cortex neurogenesis. Cecilia Lopez, Katherine Saud, Daniela Peña, and Manuel Kukuljan. Biomedical Neuroscience Institute (BNI), Facultad de Medicina, Universidad de Chile. (119) BDNF is expressed in a Wnt/β-catenin-dependent manner in hippocampal neurons and HT-22 cell line. Cristobal Mejías-Reyes & Nibaldo C. Inestrosa. Center for Aging and Regeneration (CARE), Department of Cell and Molecular Biology, Faculty of Biological Sciences, P. Catholic University of Chile. cristobal.mejias@gmail.com (121) Non-canonical Wnt signaling increases AMPA receptor clusters in hippocampal neurons. Carla Montecinos-Oliva & Nibaldo C. Inestrosa. Center for Aging and Regeneration (CARE), Department of Cell and Molecular Biology, Faculty of Biological Sciences, P. Catholic University of Chile. cjmontec@uc.cl (123) Wn5a ligand activates the mitochondrial fission protein Drp1 in hippocampal neurons. Daniela Ordenes and Nibaldo C. Inestrosa. Center for Aging and Regeneration (CARE), Department of Cell and Molecular Biology, Faculty of Biological Sciences, P. Catholic University of Chile, Chile. irma.ordenes.i@mail.pucv.cl (125) Blockade of Aβ-induced synaptotoxicity by a pentapeptide derived from the glycine zipper region. Peters C 1, Fernandez-Perez EJ 1, Burgos CF 1, Espinoza MP 1, Castillo C 1, Urrutia JC 1, Opazo C 2, Aguayo LG 1,3. 1 Laboratory of Neurophysiology, Department of Physiology, University of Concepción, Chile. 2 Kenneth Myer Building, 30 Royal Parade, The University of Melbourne, Parkville, Victoria 3010, Australia. laguayo@udec.cl carlos.opazo@unimelb.edu.au (127) Differential proliferation rates observed for human fibroblasts correlates with the reported inverse association between cancer and Alzheimer disease. 1 Ponce DP, 1,4 Salech F, 1 Merino D, 3 Henríquez M, 2,3 Quest AFG, 1,2,3 Behrens MI. 1 HCUCH, 2 CEMC, 3 ICBM, 4 BNI, Faculty of Medicine, Universidad de Chile. dponcedelavega@gmail.com (129) The hypoxia factor Hif-1α is necessary for the outgrowth of peripheral axons of primary sensory neurons. Santander L. and Reyes A.E. Laboratorio Biología del Desarrollo, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Santiago, Chile. arielreyes@unab.cl (131) Influence of Schwann cells on single neuromast regeneration in zebrafish larvae. Mario Sánchez, Daniela Gutiérrez, Miguel Allende. FONDAP CRG, Facultad de Ciencias, Universidad de Chile. allende@uchile.cl (133) Dissecting the segmentation process: A whole embryo culture approach to understand body segmentation in the beetle Tribolium castaneum. Loreto López-Gutiérrez, Viviana A. Nuñez, Gabriel Maldonado, Constanza Macaya, Patricio Saavedra, Rodrigo Cepeda & Andres F. Sarrazin. Institution: Instituto de Química, P. Universidad Católica de Valparaíso. carter.loreto@gmail.com - andres.sarrazin@ucv.cl (135) Drosophila Frazzled regulates Hedgehog Signaling. 1,2 Luis Solano R., 1 Consuelo Ibar V., 2 Verónica Palma A. and 1 Álvaro Glavic M. 1 Laboratory of Developmental Biology. 2 Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences, University of Chile. Lmasolano@gmail.com (137) Ric-8A regulates Gα13 signaling during neural crest cell migration in Xenopus tropicalis embryos. Toro-Tapia G., Rodríguez M., Marcellini S., Torrejón M. Laboratorio Genética Molecular Dpto. Bioquímica y Biología Molecular, Universidad de Concepción. gabrielatoro@udec.cl