XII International Workshop on Sensors and Molecular Recognition 5 y 6 de Julio de 2018

Transcripción

1 XII International Workshop on Sensors and Molecular Recognition 5 y 6 de Julio de 2018 Salón de grados Facultad de Farmacia

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3 COMITÉ ORGANIZADOR PRESIDENTA: Ana Mª Costero Nieto Vocales: Margarita Parra Alvarez Pablo Gaviña Costero Raúl Gotor Candell Samuel Ceballos Fernandez María Manconi Carlos Martínez Aquino Estefanía Almenar Sanchez Eva Mª Brun Sánchez Tania Godoy COMITÉ CIENTÍFICO Ana Mª Costero Ramón Martínez-Mañez Rurack Knut Jurriaan Huskens Carla Caddeo Salvador Sagrado Vives Matilde Merino Sanjuan 3

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5 INDICE Página Programa Científico 7 Conferencias Plenarias 11 Comunicaciones Orales 23 Comunicaciones en Póster 45 Relación de participantes 147 5

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7 PROGRAMA CIENTÍFICO Jueves, 5 de Julio de 2018 SESIÓN DE MAÑANA h Entrega de Documentación h Apertura Presidida por la Vicerrectora de Innovación y Transferencia de la Universitat de Valencia, Mª Dolores Real García h Conferencia Plenaria Multivalent interactions: From molecular design to biological function Jurriaan Huskens h Primera Sesión de Comunicaciones Orales: Moderador: Ana María Costero Nieto O-01 Revisiting the synthesis of magnetic mesoporous silica Santiago Sánchez Cabezas, Vicent Esteve Moya, Félix Sancenón Galarza y Ramón Martínez-Máñez O-02 Nanopartículas de oro funcionalizadas con ciclodextrinas para la detección de neurotransmisores Jonathan Álvarez García, Carlos Martínez Aquino, Ana M. Costero Nieto, Pablo Gaviña Costero O-03 Bacterial Population Control by Nanostructured Macroscopic Materials Roser Montagud-Martinez, Guillermo Rodrigo, Rafael Ballesteros-Garrido h Pausa Café h Segunda Sesión de Comunicaciones Orales: Moderador: Pablo Gaviña Costero O-04 Sensores para la detección de CO basados en la generación de cumarinas María Benítez, Estefanía Almenar, Pablo Gaviña, Ana M. Costero 7

8 O-05 Inhibitor effect of a fluoromethychalcone derivative on caspase-1 activation in msu-induced gouty arthritis model Laura Catalán, Mª Carmen Terencio, Mª Luisa Ferrándiz, Mª José Alcaraz O-06 Modified carbon foams with sensing properties for the detection of polyphenols Cristina. Fernandez-Blanco, C. Garcia-Cabezon, M. L. Rodriguez-Mendez O-07 Photochemical anchoring of thiolated nucleic acids to C-F bond on surfaces for microarraying Pilar Jiménez-Meneses, María-José Bañuls, Rosa Puchades, Ángel Maquieira h Conferencia Plenaria h Comida SESIÓN DE TARDE Glycodendritic structures as tools in chemical biology Javier Rojo h Conferencia Plenaria New antigenic determinants and nanostructures for diagnosis of IgE-mediated drug allergic reactions María Isabel Montañez Vega h Tercera Sesión de Comunicaciones Orales Moderador: Angel Maqueira O-08 Diseño de un escáner low cost basado en sensor RGB Jorge Lorente Benítez, Patricia Noguera, Pilar Aragón, Rafael Masot, Miguel Alcañiz O-09 enose technology for non-invasive diagnostic of prostate cancer. Preliminary study Talens Felis, Juan.B; Sebastià Fabregat, N.; Pelegrí-Sebastià, J.; Sogorb Devesa, T.; Loras Monfort, A.; Ruiz-Cerdá, J.L. O-10 Nanosensor based on enzyme-mediated labelled-oligonucleotide release from Au-mesoporous silica nanoparticles for the fluorometric detection of urea Antoni Llopis-Lorente, Reynaldo Villalonga, Ramón Martínez-Máñez, and Félix Sancenón O-11 Caracterización de rutas metabólicas alteradas en el cáncer de vejiga a través de análisis metabolómicos y transcriptómicos de muestras tisulares e identificación de metabolitos urinarios como potenciales biomarcadores 8

9 Alba Loras, Mª Carmen Martinez, Jesús Maria Paramio, Guillermo Quintás, Salvador Gil, Ramón Martinez, Cristian Suarez, José L. Ruiz- Cerdá O-12 Detection of mrna biomarkers using graphene oxide and upconversion nanoparticles María Isabel Lucío, Davide Giust, Patrick Vilela, Afaf H. El-Sagheer, Tom Brown, Lorraine E. Williams, Otto L. Muskens, Antonios G. Kanaras h Sesión de Pósters Viernes, 6 de Julio de 2018 SESIÓN DE MAÑANA h Conferencia Plenaria Stem cell-niche biology in the adult brain Isabel Fariñas h Cuarta Sesión de Comunicaciones Orales: Moderador: Salvador Gil Grau O-13 Ring-assisted enantioselective synthesis of interlocked β-lactams Alberto Martinez-Cuezva, Mateo Alajarin, Jose Berná O-14 Passion fruit-like nano-architectures: synthesis, applications and perspectives as sensors Salvador Pocoví-Martínez, Domenico Cassano and Valerio Voliani O-15 Desarrollo de sensores para la detección de neurotransmisores en medios biológicos Silvia Rodríguez, Samuel A. Ceballos, Margarita Parra, Ana M. Costero h Pausa Café h Quinta Sesión de Comunicaciones Orales: Moderador: Virginia Merino Sanjuan O-16 In-situ biofunctionalization and label-free protein detection using a nanophotonic biosensor Jad Sabek, Luis Torrijos-Morán, María-José Bañuls, Zeneida Díaz- Betancor, Ángel Maquieira, Jaime García-Rupérez 9

10 O-17 Study of the photoisomerization of a rotaxane-based molecular shuttle with a non-conventional topology Adrian Saura-Sanmartin, Alberto Martinez-Cuezva, Aurelia Pastor, Mateo Alajarin, Jose Berna O-18 Study of water behaviour with dielectric spectroscopy of poultry meat during hot air drying J. A. Tomas-Egea, M. Castro-Giraldez, R.Colom, P. J. Fito O-19 Optimizing Procedures for Isolation and Separation of Extracellular Vesicles from Adipose Tissue-derived Mesenchymal Stem Cell Vázquez Mª José, Tofiño-Vian M, Guillén MI and Alcaraz MJ h Conferencia Plenaria h Clausura Functionalized nanomedicines for cell-driven recognition drug delivery Bruno Sarmento SESIÓN DE PÓSTERS Los pósters deberán estar colocados: desde el Jueves 5 de Julio a las 9.30h hasta el Viernes 6 de Julio a las 13.45h. LIBRO DE RESÚMENES El plazo de presentación de los resúmenes de las comunicaciones para la edición del libro finalizará el próximo día 17 de Septiembre. El formato debe ser como los abstracts pero con una extensión de 4 o 5 hojas. 10

11 CONFERENCIAS PLENARIAS 11

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13 Multivalent interactions: From molecular design to biological function Jurriaan Huskens University of Twente, MESA+ Institute for Nanotechnology, Molecular Nanofabrication group, Enschede, The Netherlands Multivalency is the phenomenon that describes the interaction between multivalent receptors and multivalent ligands. It is well known to play a pivotal role in biochemistry, particularly in proteincarbohydrate interactions, both in solution (e.g. at pentavalent cholera toxins) and at interfaces (e.g. for the infection of cells by the attachment of viruses or bacteria to cell membranes). 1-3 In particular in the latter case, multivalency is often poorly understood in a quantitative sense. 4 Supramolecular host-guest chemistry has been well established in solution, but its use at interfaces remains limited to for example sensor development for specific guest compounds. In order to build assemblies at surfaces through supramolecular interactions for nanotechnological applications, other demands have to be met, such as larger thermodynamic and kinetic stabilities of the assemblies. For many supramolecular motifs, this inevitably leads to the use of multivalent interactions. 2,4 A key point of the current presentation will be the transition area between slowly and rapidly exchanging multivalent interactions, and their influence on the dynamics and overall functioning of supramolecular systems, both in solution and on surfaces. It will be explained how this concept can lead to the design of artificial systems (see Figure) to study the interaction between influenza and a cell surface, which together provide a deeper understanding of this interaction. References 1. M. Mammen, S.-K. Choi, G. M. Whitesides, Angew. Chem. Int. Ed. 1998, 37, A. Mulder, J. Huskens, D. N. Reinhoudt, Org. Biomol. Chem. 2004, 2, C. Fasting, C. A. Schalley, M. Weber, O. Seitz, S. Hecht, B. Koksch, J. Dernedde, C. Graf, E.-W. Knapp, R. Haag, Angew. Chem. Int. Ed. 2012, 51, J. Huskens, A. Mulder, T. Auletta, C. A. Nijhuis, M. J. W. Ludden, D. N. Reinhoudt, J. Am. Chem. Soc. 2004, 126,

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15 GLYCODENDRITIC STRUCTURES AS TOOLS IN CHEMICAL BIOLOGY Javier Rojo 1 1 Instituto de Investigaciones Químicas, CSIC Universidad de Sevilla. Av. Américo Vespucio 49, Seville, Spain javier.rojo@iiq.csic.es Carbohydrates, Lectins, Molecular Recognition Glycans encode a large amount of molecular information that is decoded by particular receptors named lectins, proteins able to read out this information through the interaction with these glycans. These glycans play a key role in several natural and pathological processes such as embryogenesis, cell-differentiation, inflammation, tumor metastasis, etc. Their interaction with lectins is highly selective but weak although Nature overcomes this weak affinity by a multivalent presentation of carbohydrates [1]. The complexity of the glycan structures present in nature, their heterogeneity, and the difficulty to synthesize in the laboratory these complex molecules in sufficient amount to perform functional studies demand strategies to generate simple but efficient synthetic models. Moreover, these synthetic models have to be multivalent tools to study and intervene in these biological processes where carbohydrates are involved. Our group has synthesized dendritic structures functionalized with carbohydrates using several scaffolds such as small molecules, fullerenes or viral-like particles affording glycodendritic structures with different size, valency, presentation, and type of ligand. The activity of these glycodendritic structures has been evaluated in cell infection models [2] as well as in the development of synthetic vaccines against allergy [3]. References [1] A. Varki. Glycobiology. 2017, 27,3-49. [2] a) J. Luczkowiak, et al. Bioconjugate Chem. 2011, 22, ; b) R. Ribeiro- Viana, et al. Nature Commun. 2012, 3:1303 ; c) J. Luczkowiak, et al. Biomacromolecules 2013, 14, ; d) A. Muñoz, el al. Nature Chem. 2016, 8, 50-57; e) B.M. Illescas, J. Rojo, R. Delgado, N. Martín. J. Am. Chem. Soc., 2017, 139, [3] a) W. Kowalczyk, A. Mascaraque, M. Sánchez-Navarro, J. Rojo, D. Andreu. Eur. J. Org. Chem. 2012, ; b) A. Mascaraque, W. Kowalczyk, T. Fernández, L. Mayorga, D. Andreu, J. Rojo. MedChemComm 2015, 6, ; c) M.J. Rodríguez, et al. Mol, Nut. Food Res. 2017, 61,

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17 New antigenic determinants and nanostructures for diagnosis of IgEmediated drug allergic reactions Maria Isabel Montañez IBIMA-Regional University Hospital of Malaga-UMA. Málaga, Spain BIONAND Andalusian Centre for Nanomedicine and Biotechnology. Málaga, Spain Drugs are low molecular weight substances which cannot cause an immune response unless they are covalently conjugated to proteins. The nature of both the carrier molecule and the antigenic determinant will influence immunoglobulin E (IgE) molecular recognition. On one hand, understanding how the drug is metabolized after protein conjugation is important to advance diagnosis of clinical allergy. Structural studies of betalactam-protein adducts are difficult to perform and for some drugs have never been addressed successfully. Our approach is to identify the antigenic determinant structures by designing and synthesizing the proposed skeletons that remain linked to the carrier protein after chemical degradation of the drugs (cephalosporin, clavulanic acid). On the other hand, synthetic hapten-carrier conjugates, structurally comparable to those formed in vivo (hapten-protein), with a structural control and a reproducible manner of preparation are considered necessary for the development of diagnostic tools. The structural precision and tunable properties of dendrimers can be used for this task. This talk will be focused on progress in the characterisation of new antigenic determinants and materials for immunoassay applications, focusing on the development of methodologies to prepare chemically controlled and reproducible dendritic substrate surfaces. These structures appear to mimic conjugates formed in vivo. Moreover, they can be used for in vitro quantification of IgE, thus avoiding the need for invasive in vivo tests. Relevant conclusions in terms of diagnosis can be obtained from clinical evaluation of these materials. 17

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19 Stem cell-niche biology in the adult brain Isabel Fariñas 1 Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), 2 Departamento de Biología Celular, Biología Funcional y Antropología Física 3 Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BIOTECMED), Universidad de Valencia, Burjassot, Spain. Adult stem cells are found at specific locations and their behavior and lifelong maintenance is regulated by both cell intrinsic factors and signals from the microenvironment or niche in which they reside. Astrocyte-like neural stem cells (NSC) continually produce new neurons and oligodendrocytes in two discrete neurogenic niches of the adult mammalian brain, the subgranular zone and the subependymal zone (SEZ). The potential of NSCs for brain repair is fueling the concept of stem cell niches as druggable targets for restorative therapies. However, stem cell niches are still poorly characterized due to the complexity of the interactions between stem cells and their neighbors and to the dynamic changes required for the continuous production of new cells. In the adult SEZ, different elements, including innervation, irrigating vasculature and the cerebrospinal fluid, appear to play important roles in the regulation of NSC behavior, but the signalling pathways involved are still under investigation. Despite the presence of a blood-brain physical barrier, NSC behavior can be modulated also by circulating factors, in ways that are far from understood. In addition, increasing evidence indicates that remote lesions outside the central nervous system can activate NSCs through the engagement of systemic innate immune responses that are transduced to the brain. Therapeutic approaches for the activation of endogenous NSCs will necessarily require ways to deliver specific signals to neurogenic niches. Funding: MINECO/ISCIII (SAF, CIBERNED, TERCEL) and Generalitat Valenciana (Prometeo). Work in the laboratory of I.F. is supported by the Fundación Botín-Banco Santander. 19

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21 Functionalized nanomedicines for cell-driven recognition drug delivery Bruno Sarmento 1 i3s -Instituto de Investigação e Inovação em Saúde and INEB - Instituto de Engenharia Biomédica, Portugal 2 CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Instituto Universitário de Ciências da Saúde Portugal. keywords: Cell-Material interaction; Drug delivery; Functional nanomedicines; Targeted nanoparticles In drug delivery field, bioavailability and specificity are key challenges in the establishment of advanced products. Nanoparticles have been proposed by our group as valid approaches to provide successful systems to deliver drugs to their site of action. Besides the proper control of nanoparticle matrix to provide a suitable release of drug payload, the surface of nanoparticles has a major impact on the interaction with biological barriers and cell membranes. We have studied thoroughly the interaction of nanoparticles with cells and mucus regarding their adhesive properties that modulates their mucoadhesive behaviour, ultimately related with passive targeting to mucosae. Understanding how nanosystems interact with individual mucin chains and the 3D structure of mucus is paramount, as a passive functionalization of nanoparticles may concerns, exploring different biomaterials as mucus-modulators. Our active targeting approach for nanoparticles has been focused on ligand molecules attached to the surface of nanoparticles to increase the probability of binding to unregulated cell membrane receptors in key local effector sites. New and lessexplored receptors are being targeted in engineered nanosystems, providing enhanced local and intracellular levels of drugs, without compromise the safety of the systems. In this talk, application of nanosystems for mucosal delivery of drugs with physiological and social impact, developed in our research group, will be presented. The surface modification of nanomaterials with targeting moieties, from biological biomacromolecules to biopolymers, has been successfully attained. 21

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23 COMUNICACIONES ORALES 23

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25 O-01 Revisiting the synthesis of magnetic mesoporous silica nanoparticles Santiago Sánchez Cabezas 1, Vicent Esteve Moya 2, Félix Sancenón Galarza 1,3 y Ramón Martínez-Máñez 1,3 1 Instituto Inter. de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València. Camino de Vera s/n Valencia, Spain- santiago.sanchez@idm.upv.es 2 AVMcybernetics and Microsystems 3 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud Carlos III Nanomedicine is a multidisciplinary area that applies nanotechnology to solve some of the problems that medicine is currently facing. In particular, the use of nanoparticles has revolutionized the study of biological processes at the cellular and molecular level and has provided an attractive tool for the treatment and diagnosis of different diseases. 1 Accordingly, the design and production of this nanodevices has grown exponentially in the last decades, leading to a whole range of nanoparticles with all kind of compositions, sizes and properties. 2 However, the mounting number of publications dealing with the design of new nanodevices has not been reflected in the number of approved clinical treatments, showing a poor translation of results from the lab to the clinic. 3,4 One of the reasons for this is the difficulty of predicting how a specific nanodevice is going to interact with a living organism. The design of effective nanodevices relies on a better understanding of cell-nanoparticle interactions, which requires nanoparticles with well-defined physico-chemical properties such as size, composition and surface chemistry. To control these properties at the nanoparticle level results increasingly challenging due to the complexity of new multifunctional nanodevices. For this reason, reliable synthetic procedures are required. The scope of this project was to study the synthesis of core-shell magnetic mesoporous silica nanoparticles (M-MSNs), which are attractive multi-functional nanodevices for both therapeutic (drug delivery, hyperthermia) and diagnostic applications (MRI, optical imaging). 5 Reaction parameters were investigated in order to identify those which have a major contribution on the final nanoparticle physico-chemical properties. Optimization of these reaction parameters led to monodisperse M-MSNs with uniform size and shape. The resulting nanoparticles were fully characterized and are presented as multi-functional nanodevices with promising applications in nanomedicine. (1) Taylor-Pashow, K. M. L.; Della Rocca, J.; Huxford, R. C.; Lin, W. Chem. Commun. (Camb). 2010, 46 (32), (2) Khan, I.; Saeed, K.; Khan, I. Arab. J. Chem (3) Zhang, R. X.; Li, J.; Zhang, T.; Amini, M. A.; He, C.; Lu, B.; Ahmed, T.; Lip, H.; Rauth, A. M.; Wu, X. Y. Acta Pharmacol. Sin. 2018, 39 (5), (4) Hare, J. I.; Lammers, T.; Ashford, M. B.; Puri, S.; Storm, G.; Barry, S. T. Adv. Drug Deliv. Rev. 2017, 108, (5) Wang, Y.; Gu, H. Adv. Mater. 2014,

26 O-02 NANOPARTÍCULAS DE ORO FUNCIONALIZADAS CON CICLODEXTRINAS PARA LA DETECCIÓN DE NEUROTRANSMISORES Jonathan Álvarez García 1,2, Carlos Martínez Aquino 1,2, Ana M. Costero Nieto 1,2,3, Pablo Gaviña Costero 1,2,3 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM). Universidad Politècnica de València, Universitat de València, Doctor Moliner 50, Burjassot, 46100, Valencia, Spain. 2 Departamento de Química Orgànica, Universitat de València, Doctor Moliner 50, Burjassot, 46100, Valencia, Spain. 3 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain. Palabras clave: Nanopartículas de oro, ciclodextrinas, neurotransmisores Los neurotransmisores son moléculas endógenas, de estructura y complejidad variada, que actúan como mensajeros químicos durante la sinapsis. En el cuerpo humano, desajustes en los niveles normales de estos compuestos se relacionan con diferentes enfermedades, como pueden ser la Esquizofrenia o el Parkinson [1]. Es por ello que la detección de neurotransmisores se ha convertido en un campo de especial interés. Por otro lado, las nanopartículas de oro (AuNPs) han sido ampliamente utilizadas en el desarrollo de sensores colorimétricos, debido a sus especiales propiedades optoelectrónicas [2]. Las ciclodextrinas (CD), oligosacáridos cíclicos compuestos por unidades de α-d-glucopiranosa, han demostrado poseer grandes propiedades para formar complejos de inclusión, constituyendo una herramienta de gran utilidad en la química supramolecular [3]. En este trabajo se propone el desarrollo de sistemas basados en nanopartículas de oro funcionalizadas con ciclodextrinas, de manera que la formación de complejos de inclusión 2:1 con los neurotransmisores induzca la agregación de las AuNPs y consecuentemente se dé un cambio de color en la disolución. Referencias [1] Jonathan W. Steed, Jerry L. Atwood. Supramolecular Chemistry. 2 nd Edition, John Wiley & Sons, 2009 [2] Y. Yeh, B. Creran, V.M. Rotello. Nanoscale, 2012, 4, [3] G. Crini, Chem. Rev. 2014, 114,

27 O-03 Bacterial Population Control by Nanostructured Macroscopic Materials Roser Montagud-Martinez, Guillermo Rodrigo, Rafael Ballesteros-Garrido I2SysBio CSIC/UVEG C/ Catedrático José Beltrán, Paterna València rafael.ballesteros-garrido@uv.es 3 keywords: Metal-Organic Frameworks, Drug Release, Cell Growth The uncontrolled bacterial propagation is at the base of many significant diseases. The most studied treatments focus on the administration of antibiotics, orally, intravenously and in some cases cutaneous. However, this does not always give good results because the effect of the antibiotic does not act decisively on the focus of infection or because the bacteria are resistant. The nanomaterials have emerged in recent years as new efficient and programmable elements for the distribution of biochemical compounds on demand in different biological contexts. [1] These developments have focused on obtaining nanometer-scale materials, which are difficult to control once disseminated. Herein we explore the construction of macroscopic and nanostructured materials based on copper, which can serve for long time copper liberation and also as containers for antibiotic molecules. Certainly, the development of such materials offers new biomedical horizons. [2] Because of the inherent properties of copper, biocide and antiviric activity, the construction meta stable copper-based materials that slowly degrade yielding to free copper (II) atoms presents an important challenge. [1] E. Aznar, M. Oroval L. Pascual, JR, Murguía, R. Martínez-Máñez, F. Sancenón Chem. Rev. 2016, 116, [2] P. Horcajada, T. Chalati, C. Serre et al. Nature Mat. 2010, 9,

28 O-04 SENSORES PARA LA DETECCIÓN DE CO BASADOS EN LA GENERACIÓN DE CUMARINAS María Benítez 1, Estefanía Almenar 1, Pablo Gaviña 1,2,3, Ana M. Costero* 1,2,3 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València; Doctor Moliner 50, 46100, Burjassot, Valencia, España. mabebe6@alumni.uv.es 2 Departamento de Química Orgánica, Universitat de València, Doctor Moliner 50, 46100, Burjassot, Valencia, España. 3 CIBER de Bioingenierıía, Biomateriales y Nanomedicina (CIBER-BBN), España. Palabras clave: CO, Cumarina, Complejo paladio El monóxido de carbono es un gas neurotransmisor que se produce mediante el metabolismo del grupo hemo y que juega un papel importante en la regulación de funciones celulares y psicológicas. De esta forma, su detección en sistemas biológicos supone un gran interés en diversos campos científicos. Las cumarinas son compuestos orgánicos que presentan una propiedad denominada two-photons absortion (TPA), es decir, llevan a cabo la absorción simultánea de dos fotones para pasar del estado fundamental al excitado. Así, la energía emitida por estos compuestos es mayor a la absorbida. A partir de esto, se ha llevado a cabo la síntesis de dos sensores basados en complejos de paladio tetracoordinados. Al colocar estos complejos en contacto con el CO, se espera la inserción de este y la posterior eliminación reductora que daría lugar a la formación de la cumarina. Debido a que el complejo precursor no posee propiedades TPA y sí las cumarinas, la aparición de este fenómeno puede ser usado para verificación de detección de CO. CF 3 CO CF 3 N N O Pd N N O O CO Ph N Pd O N O O Referencias [1] N. Abeyrathna, K. Washington, C. Bashur, Y. Liao. Org. Biomol. Chem. 2017, 15, [2] K. Sasano, J. Takaya, N. Iwasawa. J. Am. Chem. 2013, 135, [3] J. Ferguson, F. Zeng, H. Alper. Org. Lett. 2012, 14,

29 O-05 INHIBITOR EFFECT OF A FLUOROMETHYCHALCONE DERIVATIVE ON CASPASE-1 ACTIVATION IN MSU-INDUCED GOUTY ARTHRITIS MODEL Laura Catalán 1,2, Mª Carmen Terencio 1,2, Mª Luisa Ferrándiz 1,2, Mª José Alcaraz 1,2. 1 Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Av. Vicent Andrés Estelles S/N Burjassot (València) 2 IDM, Instituto Interuniversitario de reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat de València, Dr. Moliner,50, Burjassot (València) laura.catalan@uv.es keywords: Caspase-1; MSU; gouty arthritis model The inflammatory process includes the participation of many factors, such as the proinflammatory cytokine IL-1β, which require post-translational processing by active caspase-1 to generate their active forms. Caspase-1 activity is controlled by inflammasomes, a group of cytosolic protein complexes whose activity has been linked to many diseases including arthritis. This complex is activated by microbial pathogens and damage-associated molecular patterns such as monosodium urate crystals (MSU) [1]. Previous studies demonstrated the potential anti-inflammatory profile of 3,4,6-trimethoxy- 6 -trifluoromethylchalcone (CH) in the mouse air pouch model [2]. In the present study, we have evaluated the mechanism of action of CH in mouse gouty arthritis model in order to determine its regulation on caspase-1 activation. Mice were orally administered with 0.2 ml Tween80/etanol/water (5:5:90) containing CH (30mg/kg) or vehicle. After 1 h, MSU crystals or PBS were subcutaneously injected under the plantar surface of the right paw. Paw edema was quantified at 1, 3, 6 and 24 h after MSU injection by volumetric assay using Digital Water Plethysmometer. Then, mice were sacrificed and paw tissue was homogenized and centrifuged. The supernatant was collected for MPO assay, detection of IL-1β, CXCL-1, IL-6 and TNF-α by ELISA and immunoblot assay of active p20 subunit of caspase-1. Results demonstrated that oral administration of CH significantly reduced foot edema, as well as IL-1β (42%), TNF-α (36%), IL-6 (64%) and CXCL-1 (36%) levels in supernatants. Furthermore, MPO was also significantly inhibited after exposition to CH (31%). Besides, the decrease in the expression of activated p20 subunit after CH administration, suggests that inhibition of caspase-1 activation is a potential mechanism of CH anti-inflammatory effect. References: [1] H.E. Lee, G. Yang, N.D. Kim, S. Jeong, Y. Jung, J. Y. Choi, H. H. Park, J.Y. Lee. Scientific Reports. 2016, col. 6,6: [2] L. Catalan, M.C. Terencio, M.L. Ferrandiz, M.J. Alcaraz. Basic & Clinical Pharmacology & Toxicology. 2017, 121,

30 O-06 Modified carbon foams with sensing properties for the detection of polyphenols C. Fernandez-Blanco 1, C. Garcia-Cabezon 2, M. L. Rodriguez-Mendez 1 1 Group UVaSens, Engineers School, Universidad de Valladolid, Valladolid (Spain) anacristina.fernandez@uva.es 2 Departamento de Ingeniería de Materiales, Engineers School, Universidad de Valladolid, Valladolid, (Spain) 3 keywords: electroactive modifiers, carbon foam, catechol Sensors have been employed for the detection of antioxidants presented on food. Some of these antioxidants are phenolic acids, flavonoids or cinnamic acids [1]. To get better electrochemical activity for a sensor towards the determination of polyphenols, the electrodes could be modified with different electroactive materials, such as metallic phthalocyanines, metallic nanoparticles, carbon nanotubes or enzymes [2]. In this case, as electrodes are used carbon foams that present high electroactive area and an increase in the surface coverage (Г). For that reason, carbon foams without modifications and carbon foams with different electrocatalytic materials have been studied, given to the sensor better properties towards polyphenols determination. On the other hand, in order to study the effect of the pore size for the foam in its electrochemical behavior, foams with two pore sizes have been used in catechol determination: 20 pores per inch and 100 pores per inch, where the first one presents a higher pore size than the second one. Figure 1. Square wave voltammetry recorded for carbon foams modified with electrocatalytic compounds with a pore size of 20 PPI (a) and a pore size of 100 PPI (b). Acknowledgments Financial support by MINECO-FEDER (AGL R) and the JCyL-FEDER (VA-011U16) is gratefully acknowledged. C. Fernandez-Blanco thanks to JCyL-FEDER for a postdoctoral fellowship (VA-011U16). References [1] P. A. Kilmatin, H. L. Zou, A. L. W. American Journal of Enology and Viticulture. 2002, 53, 294. [2] F. J. Pavinatto, E. G. R. Fernandes, P. Alessio, C. J. L. Constantino, J. A. de Saja, V. Zucolotto, C. Apetrei, O. N. Oliveira Jr, M. L. Rodriguez-Mendez. Journal of Materials Chemistry. 2011, 21,

31 O-07 Photochemical anchoring of thiolated nucleic acids to C-F bond on surfaces for microarraying Pilar Jiménez-Meneses 1, María-José Bañuls, 1,2 Rosa Puchades, 1,2 Ángel Maquieira 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Camino de Vera s/n, E46022 Valencia, Spain. pijime@upvnet.upv.es 2 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain Keywords: Fluor-thiol photoreaction, fluorinated surfaces, nucleic acid microarray Microarray is an interesting technology in fields as biology or biomedicine for both, prevention and detection tests. There are still several challenges to be achieved, as the better understanding of the process occurring at the liquid-solid interface, and the assay reproducibility, and sensitivity. One critical issue in the microarray performance is the way the probes are anchored to the solid support. Here, a novel fluor-thiol coupling reaction has been demonstrated and applied to nucleic acid anchoring on substrate surfaces. This new photochemical reaction allows to anchor thiolated nucleic acid probes to C-F motifs present on the different material surfaces. Just with 30 seconds of irradiation, at 254 nm, the probes are covalently linked to the surface. Then, knowing the low reactivity of fluorinated surfaces, this novel reaction is of huge interest as it provides high immobilization densities (up to 40 pmol/cm 2 ) onto highly repellant surfaces. That significantly minimizes the background, and the unspecific adsorption. Analogous to the thiol-ene coupling reaction, extensively applied in the last years to microarray technology, the fluor-thiol reaction shows the advantages of the click chemistry reactions, as well [1, 2]. Figure 1. Fluorescence image of an array with thiolated probe immobilized onto a perfluorinated surface. References [1] Escorihuela, J.; Bañuls, M. J.; Puchades, R.; Maquieira, Á. Chem. Commun. 2012, 48, [2] Bañuls, M.-J.; Jiménez-Meneses, P.; Meyer, A.; Vasseur, J.-J.; Morvan, F.; Escorihuela, J.; Puchades, R.; Maquieira, A. Bioconjug. Chem. 2017, 28, Acknowledgments Financial support from INTERBOINTER (project CTQ R) and BIHOLOG (Project CTQ R), FEDER and GVA PROMETEO II 2014/040 is acknowledged. P. J.-M. acknowledges the Spanish Ministry of Economy, Industry and Competitiveness for the public FPI grant (Project CTQ R) and the co-financing by the European Social Fund. 31

32 O-08 DISEÑO DE UN ESCÁNER LOW COST BASADO EN SENSOR RGB Jorge Lorente Benítez, Patricia Noguera 1, Pilar Aragón 1, Rafael Masot 2, Miguel Alcañiz 2 1 Departamento de Química. Universitat Politècnica de València. 2 Departamento de Ingeniería Electrónica. Universitat Politècnica de València. jorlobe@etsid.upv.es Palabras clave: Análisis fotométrico, sensor RGB, MATLAB Hoy en día, el análisis de muestras químicas es de vital importancia en un gran número de sectores industriales [1]. En este sentido, es preciso contar con equipos adecuados. Las soluciones existentes son muy caras y poco versátiles, dado que los dispositivos sólo admiten los kits de pruebas de su fabricante [2]. Como alternativa, se ha diseñado y fabricado un dispositivo de bajo coste para el análisis fotométrico de diversas muestras químicas a partir de un sensor RGB, el software de Arduino necesario para su control y una interfaz gráfica en MATLAB para el usuario final. El sistema permite extraer las coordenadas RGB de las muestras, graficar los resultados y realizar la extracción de los datos necesaria para la determinación de concentraciones químicas. Los datos se transmiten por conexión USB a un ordenador que tenga instalado el software diseñado. Se ha caracterizado el sistema y se ha empleado en la determinación de nitratos en agua potable, para lo cual se han comparado las lecturas con las del reflectómetro comercial RQFlex de Merck. Los resultados revelan que el dispositivo fabricado es viable para el análisis de muestras y que, tras una serie de mejoras, podría suponer una alternativa real a los sistemas comerciales actuales. Ilustración 1: Sistema mecánico, prototipo montado y aplicación para su control. Referencias [1] Dr Bitter et al. Elemente Chemie II. 5. Auflage, Ernst Klett Verlag, Köln [2] Michael Bass et al. Handbook of Optics Volume II Devices, Measurements and Properties. 2 nd edition, aaamcgraw-hill, USA

33 O-09 enose technology for non-invasive diagnostic of prostate cancer. Preliminary study. Talens Felis, J.B 1 ; Sebastià Fabregat, N. 2 ; Pelegrí-Sebastià, J. 1 ; Sogorb Devesa, T. 1 ; Loras Monfort, A. 2 ; Ruiz-Cerdá, J.L. 1 1 Sensors and Magnetism Group, Universitat Politècnica de València-Campus de Gandia.. 2 Unidad Mixta de Nanomedicina y Sensores, Instituto de Investigación Sanitaria La Fe. 3 keywords: prostate cancer, enose, biomarker INTRODUCTION: Volatile organic compounds (VOCs) are products of tumor cellular metabolism present in biofluids of various cancers. In prostate cancer (PCa), an indirect evidence of their presence is the high sensitivity of canine smell to discriminate urine from PCa patients observed in some studies. An alternative to this is the enose technology, which uses sensors that, when VOCs passes through them, leave a specific volatile chemical trace. Fingerprints are added by a preprocessor and software that, by comparison with a database, identifies characteristic VOCs. OBJECTIVE: Proof of concept for the application of enose technology for detection of VOCs in urine of PCa patients. MATERIAL AND METHODS: Urinary samples from 20 patients with PCa (cases) and 20 patients with Benign Prostate Hyperplasia (BPH) (controls) were analyzed with the 32- sensors enose system using metal oxide semiconductors. Four aliquots (5 ml) were made per urine sample and each aliquot was analyzed 5 times. The parameters of 600 patterns were introduced in the software data mining WEKA. A classification of the samples was carried out through a cross-validation (k=40) and three types of classifiers were used: K-NN (K-Nearest Neighbour), Bayesian network and perceptron multilayer. For the analysis of the diagnostic performance, sensitivity, specificity and predictive positive (VP+) and predictive negative (VP-) value were determined. RESULTS: Quantitative analysis showed differences between the volatile chemical signals from PCa and BPH urine. All classifiers presented a safety diagnostic power over 75%. However, the K-NN classifier was the method with the best performance diagnosis: sensitivity=0.92 (IC95%= ), specificity=0.89 (IC95%= ), VP+=0.89 (IC95%= ) and VP-=0.92 (IC95%= ). CONCLUSIONS: Differences in the volatile urine fingerprint detected by enose between PCa and HBP urine samples as well as the diagnostic performance obtained suggest the presence of specific VOCs for PCa. This open the door to study relationship between some determined VOCs with the cancer from patients with PCa and means the first step to the development of this technology as a diagnostic method for PCa detection. REFERENCES: [1] J. Cornu, G. Cancel-Tassin, V. Ondet et al. Eur.Urol. 2011, 59, [2] M. Bernabei, G. Pennazza, M. Santonico et al. Sensors and Actuators B: Chemical, 2008, 131, 1-4. [3] A. Roine, E. Veskimäe, A. Tuokko, et al. J Urol. 2014,192 (1), This work was supported by the I+D+I program of the Generalitat Valenciana AICO/2016/046 33

34 O-10 Nanosensor based on enzyme-mediated labelled-oligonucleotide release from Au-mesoporous silica nanoparticles for the fluorometric detection of urea Antoni Llopis-Lorente, 1,2,3 Reynaldo Villalonga, 4 Ramón Martínez-Máñez, 1,2,3 and Félix Sancenón 1,2,3 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València (Spain). Camino de Vera s/n, 46022, Valencia. anllolo2@upv.es 2 Departamento de Química, Universitat Politècnica de València. Camino de Vera s/n, 46022, Valencia. 3 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) (Spain) 4 Nanosensors & Nanomachines Group, Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid. 3 keywords: nanosensor, oligonucleotide, urea A novel sensing nanodevice based on the release of Alexa Fluor 647-labelled oligonucleotide from Janus Au-MSNPs (mesoporous silica nanoparticles) mediated by an integrated enzymatic unit that is applied for the fluorometric detection of urea is reported. Janus Au-MSNPs are functionalized on the silica face with amino groups to which labelled oligonucleotide is attached by electrostatic interactions, whereas the gold face is used for the grafting of urease enzyme. The nanodevice is able to release fluorescentoligonucleotide via enzyme-mediated hydrolysis of urea to ammonia and the subsequent deprotonation of amino groups on the silica face. A fast and remarkable fluorescent signal is observed in the presence of urea with a linear response in the mm concentration range, which covers the common clinical range for urea found in serum. The nanosensor is applied for the detection of urea in real human blood samples and for the identification of adulterated milk. 34

35 O-11 CARACTERIZACIÓN DE RUTAS METABÓLICAS ALTERADAS EN EL CÁNCER DE VEJIGA A TRAVÉS DE ANÁLISIS METABOLÓMICOS Y TRANSCRIPTÓMICOS DE MUESTRAS TISULARES E IDENTIFICACIÓN DE METABOLITOS URINARIOS COMO POTENCIALES BIOMARCADORES Alba Loras 1, Mª Carmen Martinez 1,2, Jesús Maria Paramio 3,4,5, Guillermo Quintás 6,7, Salvador Gil 2,8, Ramón Martinez 1,2,9,10, Cristian Suarez 4, José L. Ruiz-Cerdá 1,11. 1 Unidad Mixta de Investigación en Nanomedicina y Sensores. Instituto de Investigación Sanitaria La Fe, Universitat Politècnica de València. Valencia, Spain albaloras@gmail.com 2 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, Universitat de València, Valencia, Spain 3 Unidad de Oncología Molecular, CIEMAT (ed70a), Madrid, Spain 4 Grupo de Oncología celular y Molecular, Hospital Universitario 12 de Octubre, Madrid, Spain 5 Centro de Investigación Biomédica en Red de Cáncer (CIBER ONC), Spain 6 Unidad Analítica, Instituto de Investigación Sanitaria La Fe, Valencia, Spain 7 Leitat Technological Center, Bio in vitro Division, Valencia, Spain 8 Departamento de Química Orgánica, Facultad de Químicas, Universitat de València, Valencia, Spain 9 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER- BBN). Spain 0 Departamento de Química, Universitat Politècnica de València, Valencia, Spain 11 Servicio de Urología, Hospital Universitario y Politécnico La Fe, Valencia, Spain 3 palabras clave: metabolómica, cáncer de vejiga, transcriptómica INTRODUCCIÓN: Debido a las elevadas tasas de recurrencia y/o progresión del CaV y las limitaciones de las técnicas diagnósticas, biomarcadores dinámicos, coste-efectivos, y no invasivos representan una necesidad [1]. La metabolómica podría utilizarse como herramienta de búsqueda de biomarcadores para el diagnóstico no invasivo de CaV [2]. MATERIAL Y MÉTODOS: Se incluyeron 22 pacientes diagnosticados de CaV. De cada uno se obtuvieron dos muestras tisulares y dos urinarias (tumoral vs no tumoral). Todas se analizaron mediante Resonancia Magnética Nuclear ( 1 H RMN). En tejidos se realizó un PLS-DA utilizando dos sets de muestras independientes (calibración (n=34) y validación (n=10)) y una posterior identificación de los metabolitos discriminantes. En orinas se realizaron dos análisis PLS-DA utilizando solamente señales de 4 metabolitos identificados como discriminantes en tejidos: uno para tumores no invasivos y otro para tumores más agresivos. Para los estudios transcriptómicos tisulares se utilizó un subconjunto de las muestras analizadas mediante 1 H RMN (n=20) y se realizó un análisis en cluster considerando las señales de los genes con un p_valor<0.05 y un Fold Change>2. RESULTADOS: En tejidos, el set de validación mostró valores de sensibilidad, especificidad, valor predictivo positivo y negativo del 100% y una curva ROC de 1. Los metabolitos discriminantes fueron principalmente aminoácidos pero también otros implicados en el metabolismo de la colina o los lípidos. Los estudios transcriptómicos de las mismas muestras validaron estos resultados. En orinas, el primer modelo presentó para la validación cruzada una sensibilidad 86.7% y especificidad 87.5%; mientras que el modelo que incluía muestras con tumores invasivos mostró para todos los parámetros estadísticos unos valores del 100%. CONCLUSIONES: Los estudios metabolomicos y transcriptomicos identificaron el metabolismo de aminoácidos y lípidos como los más importantes en el proceso de carcinogénesis vesical. Además se identificaron 4 metabolitos urinarios como potenciales biomarcadores del CaV. [1] van Rhijn BWG, Burger M, Lotan Y, Solsona E, Stief CG, Sylvester RJ, et al. From Epidemiology to Treatment Strategy. Eur Urol. 2009, 56(3)., [2] Bujak R, Struck-Lewicka W, Markuszewski MJ, Kaliszan R. J Pharm Biomed Anal. 2015, 113.,

36 O-12 DETECTION OF mrna BIOMARKERS USING GRAPHENE OXIDE AND UPCONVERSION NANOPARTICLES María Isabel Lucío 1,ǂ, Davide Giust 1, Patrick Vilela 1, Afaf H. El-Sagheer 2, Tom Brown 2, Lorraine E. Williams 3, Otto L. Muskens 1, Antonios G. Kanaras 1 ǂ Current address: a Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain and Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain 1 Physics and Astronomy, Faculty of Physical Sciences and Engineering, University of Southampton, UK 2 Department of Chemistry, Chemistry Research Laboratory, University of Oxford, UK 3 Biological Sciences, Faculty of Natural and Environmental Sciences, University of Southampton, UK 4 Institute for Life Sciences, University of Southampton, UK malube@upvnet.upv.es keywords: Biomarker detection, graphene oxide, upconversion nanoparticles Biomarkers offer valuable information about cell function and this can be used for diseases diagnostic purposes and environmental monitoring, among others. However, current techniques rely on tedious and expensive sample processing. In this scenario, nanotechnology is being explored for the development of alternative tools [1]. Within this context, our group has recently developed graphene oxide (GO)-upconversion nanoparticles (UCNPs) based optical sensors for the detection of oligonucleotides with high sensitivity [2], [3]. The assay is based on the ability of GO to quench the emission of UCNPs when bearing single strand DNA sequences. On another hand when a complementary oligonucleotide target is present, the DNA is hybridized to double strand and the GO cannot interact with the UCNPs. Thus the fluorescence intensity emission is directly related to the concentrations of the biomarker. In this communication, we report the development of the sensor and its potential to assess the presence of specific markers in biological samples. Special attention is dedicated to the last outcomes which leaded to the detection of oligonucleotides sequences within the pm range just by using a portable laser and a smartphone camera. We used the portable setup to detect mrna related to Zinc deficiency in RNA extracts from plants grown in different nutrient conditions. Our ultimate goal is to engineering a portable device for the fast, ease and reliable detection of multiple biomarkers. References [1] N. L. Rosi, C. A. Mirkin C.A., Chem Rev., 2005, 105 (4), [2] P. Vilela, A. H. El-Sagheer, T. Millar, T. Brown, O. Muskens, A. G. Kanaras. ACS Sens., 2017, 2 (1), [3] P. Alonso-Cristobal, P. Vilela, A. H. El- Sagheer, E. Lopez-Cabarcos, T. Brown, O. L. Muskens, J. Rubio-Retama, A. G. Kanaras. ACS Appl. Mater. Interfaces, 2015, 7(23), Acknowledgements: BBSRC is acknowledged for funding (BB/N021150/1). 36

37 O-13 RING-ASSISTED ENANTIOSELECTIVE SYNTHESIS OF INTERLOCKED β-lactams Alberto Martinez-Cuezva, Mateo Alajarin, Jose Berná 1 Departamento de Química Orgánica, Facultad de Química, Universidad de Murcia, 30100, Murcia (Spain) amcuezva@um.es 3 keywords: Hydrogen Bonding; Rotaxane; Enantioselective Cyclization The synthesis of mechanical interlocked molecules has received increasing attention over the last decades [1]. Within the wide range of potential applications, different research groups have focused their efforts on the study of the chemical reactivity of these systems [2]. In general, the kinetic stabilization of the functional groups located in the inner of the macrocyclic counterpart is observed. We recently found that the polyamide macrocycle in hydrogen-bonded rotaxanes proceeded as activator of a CsOH-promoted intramolecular cyclization of benzylfumaramide threads [3]. Thus a series of trans interlocked β-lactams were obtained quantitatively. In this communication, we present the synthesis of enantioenriched trans β-lactams following this methodology [4]. The macrocyclic counterpart plays an active role in the process by protecting the new lactam core against decomposition, increasing the cyclization rate, and controlling the diastereoselectivity and enantioselectivity. In contrast, the process of the unthreaded fumaramide gives low yields of a complicated mixture of products, with moderate enantioselectivities. Acknowledgments: This work was supported by the MINECO (CTQ P and CTQ P) with joint financing by FEDER Funds from the European Union, and Fundacion Seneca-CARM (Project 19240/PI/14). References [1] a) V. Balzani, A. Credi, M. Venturi. Molecular Machines Based on Rotaxanes and Catenanes, in: From Non-Covalent Assemblies to Molecular Machines, Weinheim, Wiley-VCH, 2011, 159; b) C. J. Bruns, J. F. Stoddart. The Nature of the Mechanical Bond: From Molecules to Machines, Wiley, New York, [2] E. A. Neal, S. M. Goldup. Chem. Commun. 2014, 50, [3] A. Martinez-Cuezva, C. Lopez-Leonardo, D. Bautista, M. Alajarin, J. Berna. J. Am. Chem. Soc. 2016, 138, [4] A. Martinez-Cuezva, D. Bautista, M. Alajarin, J. Berna. Angew. Chem. Int. Ed. 2018, just accepted. 37

38 O-14 Passion fruit-like nano-architectures: synthesis, applications and perspectives as sensors Salvador Pocoví-Martínez, 1 Domenico Cassano, 2,3 and Valerio Voliani 2 1 Institute of Molecular Science (ICMol), Catedrático José Beltrán Martínez nº 2, 46980, Paterna, Spain, salvador.pocovi@uv.es. 2 Center for Nanotechnology Innovation@NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro , Pisa, Italy 3 NEST-Scuola Normale Superiore, Piazza San Silvestro , Pisa, Italy Metal nanoparticles, silica, multifunctionality Passion fruit-like nano-architectures (NAs) are recently developed nature-inspired all-inone nanoplatforms [1]. NAs are biodegradable silica nanocapsules comprising biocompatible polymers and ultrasmall noble metal or magnetic nanoparticles [1,2]. NAs have demonstrated interesting features for a number of applications, among which drug delivery [2], dual ultrasound/photoacoustic imaging [3,4], and environmental remediation [5]. Figure 1. TEM image of passion fruit like nano-architectures (NAs) (center) and their main features. Clockwise from top-left: scheme of the production for all-in-one nanoplatforms, biodegradation of NAs in cellular environment, PA imaging during degradation in phantoms, and in vitro drug delivery of endogenous GSH-triggered cisplatin prodrug.[6] The synthetic protocol and the applications of NAs will be discussed together with their possible future employment as sensors. References [1] D. Cassano, J. David, S. Luin, and V. Voliani. Sci. Rep. 2016, 7, [2] D. Cassano, M. Santi, V. Cappello, S. Luin, G. Signore, and V. Voliani. Part. Part. Sys. Charact. 2016, 33, [3] C. Avigo, D. Cassano, C. Kusmic, V. Voliani, and L. Menichetti. J. Phys. Chem. C. 2017, 121, [4] P. Armanetti, S. Pocoví-Martínez, A. Flori, C. Avigo, D. Cassano, L. Menichetti, and V. Voliani. Nanomedicine NBM. 2018, [5] S. Pocoví-Martínez, D. Cassano, and V. Voliani. ACS Appl. Nano Mater. 2018, 1, [6] D. Cassano, S. Pocoví-Martínez, and V. Voliani. Bioconjugate Chem. 2018, 29,

39 O-15 DESARROLLO DE SENSORES PARA LA DETECCIÓN DE NEUROTRANSMISORES EN MEDIOS BIOLÓGICOS Silvia Rodríguez, Samuel A. Ceballos, Margarita Parra, Ana M. Costero IDM, Instituto Interuniversitario de reconocimiento Molecular y Desarrollo Tecnológico, Universitat de València CIBER de Bioingeniería, Biometariales y Nanomedicina (CIBER-BBN) (Spain) Dr. Moliner, 50, Burjassot (València). silronue@alumni.uv.es Palabras clave: NO, agregación, fluorescencia El óxido nítrico (NO) es ampliamente conocido como uno de los mayores contribuyentes a la contaminación atmosférica; no obstante, según el marco donde se encuentre, su presencia no es necesariamente negativa. Actualmente, se sabe que es un importante neurotransmisor presente tanto en mamíferos como bacterias, además de ser un elemento activo en los sistemas inmune y cardiovascular [1]. Por todo ello, lograr su detección y cuantificación se ha vuelto un campo interesante donde investigar. Para que sea posible alcanzar dicho objetivo, se ha planteado combinar la reacción de cicloadición de Huisgen entre alquinos y azidas (reacción Click ) y el fenómeno AIE (Aggregation- Induced Emission) sobre una serie de compuestos sintetizados [2,3], tomando el esqueleto del TPE (tetrafeniletileno) como armazón básico de construcción. La reacción Click está catalizada por Cu(I) y, éste, suele ser generado in situ a partir de sales de Cu(II). Existen evidencias experimentales que demuestran que el NO posee la suficiente capacidad reductora para llevar al precursor del catalizador al estado de oxidación deseado, generando así un producto que presenta fluorescencia por agregación. O O O O O O O O O O O O O O O O O O Referencias [1] Tor C. Savidge Frontiers in Neuroscience 2011, 5, 1-8. [2] Andrés Suárez An. Quím. 2012, 108, [3] Yuning Hong, Jacky W. Y. Lama and Ben Zhong Tang. Chem. Commun. 2009,

40 O-16 In-situ biofunctionalization and label-free protein detection using a nanophotonic biosensor Jad Sabek 1, Luis Torrijos-Morán 1, María-José Bañuls 2, Zeneida Díaz-Betancor 2, Ángel Maquieira 2, Jaime García-Rupérez 1 1 Nanophotonics Technology Center, Universitat Politècnica de València, Camino de Vera s/n, Valencia, 46022, Spain 2 Departamento de Química, Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico IDM, Universitat Politècnica de València, Camino de Vera s/n, Valencia 46022, Spain 3 keywords: (Nanophotonic biosensor, Thiol-ene biofunctionalization, Half antibodies) During the last years, the development and demand of label-free biosensors for a direct, rapid and cost effective analysis has rapidly increased. Within this context, we report here our work towards the development of a label-free biosensor based on nanophotonic technology for the detection of low concentrations of proteins. Photonic bandgap (PBG) sensing structures fabricated on a silicon on insulator (SOI) substrate were used, as they can provide even higher sensitivities with an extremely small footprint due to the slowwave effect occurring on them [1]. Regarding the biofunctionalization, the thiol-ene coupling (TEC) reaction was used to covalently immobilize the bioreceptors onto the surface. TEC reaction was selected because it provides 1) a more compact functionalized surface, what is translated into a higher interaction with the photonic sensing signal, and 2) a spatially-specific immobilization of the bioreceptors upon UV light excitation, what can be used to biofunctionalize each sensing region with a different bioreceptor in order to perform multiplexing [2]. Half antibodies (higgs), which were immobilized using the SH groups from their hinge region, were used as bioreceptors for the specific recognition of the target protein. To implement this biofunctionalization strategy, first an ex-situ silanization of the surface was carried out using triethoxyvinylsilane in water. The use of water as carrier for the organosilane provides several advantages such as non-vertical polymerization and sustainability. Once the sensing surface was silanized, the TEC-based immobilization of the higgs onto the photonic sensors was monitored in-situ. BSA higgs obtained by the TCEP protocol were used in these experiments [2]. The real-time monitoring of the sensing structures allowed demonstrating that the immobilization of the higgs only took place when the photonic chip was irradiated with UV light. Finally, the recognition of the target protein by the immobilized higgs was successfully measured. References [1] X. Fan, I. M. White, S. I. Shopova, H. Zhu, J. D. Suter, and Y. Sun. Anal. Chim. Acta. 2008, 620, [2] R. Alonso, P. Jiménez-Meneses, J. García-Rupérez, M.J Bañuls, Á. Maquieira. Chem.Comm (Accepted. Publication pending) 40

41 O-17 STUDY OF THE PHOTOISOMERIZATION OF A ROTAXANE-BASED MOLECULAR SHUTTLE WITH A NON-CONVENTIONAL TOPOLOGY Adrian Saura-Sanmartin, Alberto Martinez-Cuezva, Aurelia Pastor, Mateo Alajarin, Jose Berna* Departamento de Química Orgánica, Facultad de Química, Excellence Campus Mare Nostrum, Universidad de Murcia, 30100, Murcia, Spain, adrian.saura@um.es 3 keywords: mechanical bond, molecular topology, photoisomerization The mechanical bond plays a fundamental role in many processes occurring within the organism such as mitochondrial scission or DNA replication, which proceed through mechanically interlocked intermediates [1]. These processes inspired synthetic chemists for developing different strategies to synthesize compounds that emulate biological systems. In this regard, the synthesis of novel systems based on rotaxanes could be a way for the advance of smart devices controlled by a switchable mechanical motion [2]. Within the range of rotaxane-based systems, molecular shuttles are highlighted for their versatility and their greater variety of motions, resulting in several applications [3]. In this communication, a series of rotaxanes with a non-conventional topology synthesized through a clipping methodology is described. Furthermore, the tactical incorporation of two binding sites as scaffolds allows the control of the macrocycle shuttling through the application of a photochemical stimulus. Fig 1. Schematic representation of a molecular shuttle. Acknowledgments: This research was supported by MINECO (CTQ P) with joint financing by FEDER Funds from the European Union, and Fundacion Seneca-CARM (Project 19240/PI/14). A. S.-S. also thanks the Fundacion Seneca-CARM for his predoctoral contract. References [1] S. Erbas-Cakmak, D. A. Leigh, C. T. MacTernan, A. L. Nussbaumer, Chem. Rev. 2015, 115, [2] M. Xue, Y. Yang, X. Chi, X. Yang, F. Huang, Chem. Rev. 2015, 115, [3] A. Martinez-Cuezva, A. Saura-Sanmartin, T. Nicolas-Garcia, C. Navarro, R.-A. Orenes, M. Alajarin, J. Berna, Chem. Sci. 2017, 8,

42 O-18 STUDY OF WATER BEHAVIOUR WITH DIELECTRIC SPECTROSCOPY OF POULTRY MEAT DURING HOT AIR DRYING J. A. Tomas-Egea 1, M. Castro-Giraldez 1, R.Colom 2, P. J. Fito 1*. 1 Instituto Universitario de Ingeniería de Alimentos para el Desarrollo, Universitat Politècnica de València, Camino de Vera s/n, Valencia, Spain, *pedfisu@tal.upv.es 2 Instituto de Instrumentación para Imagen Molecular, Universitat Politècnica de València, Camino de Vera s/n, Valencia, Spain Keywords: Poultry meat, radiofrequency, drying Poultry meat has increased their sales in recent years due to a combination between low price and low fat content compared to pork and beef, and it is expected to be the most produced type of meat in the World [1]. In this context, it is necessary to improve the processes that are involved the poultry meat, more concretely drying. Spectrophotometry has been widely used for monitoring food processes based in permittivity [2]. Permittivity (ε) can be expressed as a complex number: the dielectric constant (ε ) is the real part, and the loss factor (ε ) is the imaginary part. The interaction of the photon flux with a biological tissue in the radiofrequency range produces two dispersions [3]: α-dispersion is caused by the orientation of mobile charges in a dielectric medium [4]; β-dispersion is related to the fixed charges orientation in macromolecules [5]. Taking this into account, the aim of this research is to develop a monitoring tool for poultry drying process using dielectric properties. The experiments were carried out drying cylinders of poultry breast in a hot air dryer. The dehydration was monitored in continuous with K type thermocouples and a thermographic camera for the temperature, a precision balance for the mass and a radiofrequency sensor connected to an Agilent 4294A impedance analyzer for the permittivity. The sensor consists of two needles with blunt-ended. Also, the moisture, volume and water activity were determined before and after the drying operation. The results allowed us to find a direct relation between the permittivity in the α-dispersion range and the number of water molecules in the surface, following the water flux and; therefore, it is possible to use the permittivity for monitoring the drying mechanisms. Referencias [1] Best, P. Worldwide Poultry Meat Production, Consumption Forecasts. Watt: Rockford, IL, USA, [2] Traffano-Schiffo, M. V., Castro-Giraldez, M., Colom, R. J., & Fito, P. J. Sensors. 2017, 17(5), [3] Schwan, H.P. Advances in Biological and Medical Physics. 1957, 5, [4] Kuang, W.; Nelson, S.O. American Society of Agricultural Engineers. 1998, 41, [5] Wolf M., Gulich R., Lunkenheimer P., Loidl A. Biochemical and Biophysical. 2012, 1824,

43 O-19 Optimizing Procedures for Isolation and Separation of Extracellular Vesicles from Adipose Tissue-derived Mesenchymal Stem Cell Vázquez MJ 1, Tofiño-Vian M 1, Guillén MI 1,2 and Alcaraz MJ 1 1 IDM. Departamento de Farmacología, Facultad de Farmacia, Universitat de València 2 Departamento de Farmacia, CEU Cardenal Herrera, Valencia vaztatmar@gmail.com Keywords: extracellular vesicles, mesenchymal stem cell, osteoarthritis Extracellular vesicles (EVs) from adipose tissue-derived mesenchymal stem cells (ASCs) might represent a therapeutic tool to treat inflammatory diseases. The immunoregulatory, anti-inflammatory and regenerative properties of ASC-EVs have been demonstrated in in vitro and in vivo models of osteoarthritis (OA). However, there is no consensus on the methods of isolation of EVs which hinders its clinical application. We have studied comparatively different isolation protocols of EVs as well as the effective antiinflammatory concentrations of these microparticles in primary cultures of OA chondrocytes. EVs were obtained from ASC conditioned medium (CM) by single-step precipitation, filtration-ultracentrifugation and size exclusion chromatography. Size and concentration were determined by resistive pulse sensing (qnano, Izon Science) both freshly obtained and after 1 and 2 freeze-thaw cycles. Finally, the effective concentrations of microvesicles (MV) and exosomes (EX) were tested on IL-6 production in primary cultures of OA chondrocytes stimulated with IL-1β. Of all isolation methods, ultracentrifugation provided the greatest recovery of EVs, and it was also the only method able to separate MV and EX subpopulations of EVs. In the filtration and centrifugation method, the changes of temperature only had influence during the centrifugation steps. In addition, successive freeze-thaw cycles significantly reduced EVs concentration after the first cycle. Finally, we established a dose-response curve for the treatment of primary cultures of OA chondrocytes with EVs. The optimal concentration to inhibit the production of the inflammatory cytokine IL-6 in OA chondrocytes was 3.6*10 7 particles/ml of MV and 7.2*10 7 particles/ml of EX. The use of ASC-EVs to treat chronic inflammatory diseases such as OA has promising advantages. The results of this work have allowed us to optimize a protocol for their experimental study. This will be of critical importance for the future development of new biomarkers and therapies based on the use of EVs derived from mesenchymal stem cells. References [1] Raposo G and Stoorvogel W (2013). Extracellular vesicles: Exosomes, microvesicles, and friends. Journal of Cell Biology. 200 (4): 373. [2] Tofiño-Vian M, Guillén MI and Alcaraz MJ (2018). Extracellular vesicles: A new therapeutic strategy for joint conditions. Biochemical Pharmacology Feb 7. pii: S (18)

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46 P-01 Influence of mechanical alloying of Ti-Ag powders in biomedical alloys obtained by powder metallurgy J. Zambrano 1, A. Dalmau Borrás 2, V. Amigó Borrás 1, J. Navarro-Laboulais 2, 1 ITM, 2 ISIRYM, Universitat Politècnica de València, Camino de Vera s/n, Valencia, SPAIN Keywords: TiAg; mechanical alloying; Corrosion Titanium is commonly used as biomaterial and Ti-Ag alloys have increased their importance due to the antibacterial behavior of silver. In this study, Ti-Ag alloys (5, 10 and 15 wt.% Ag) have been obtained by powder metallurgy. The influence of the powder mixture on their behavior: elemental blending (EB) and mechanical alloying (MA) has been analyzed. EB and MA powders have been compacted at 600 and 900 MPa, respectively, and sintered in a high vacuum oven for 3 hours at 950 C. These samples have been microstructurally characterized (SEM and XRD) and mechanically tested by means of hardness and bending tests. Electrochemical tests were carried out using a three-electrode cell in a 1M NaCl solution. Electrochemical techniques (OCP, polarization, potenciostatic tests and EIS) were employed in order to evaluate corrosion resistance. From these results, the initial conditions of the powders before sintering modify the electrochemical behavior of the Ti-Ag alloys. There are several problems with titanium alloys because they do not have antibacterial properties, due to bacterial infections occur and it's possible to lose dental or prosthetic implants, for which titanium alloys modified on its surface or alloyed with metallic antibacterial materials are investigated, copper being the most studied, besides zinc and silver [1 6] References [1]K. Oh, H. Shim, K. Kim, Properties of titanium silver alloys for dental application, J. Biomed. Mater. Res. Part B Appl. Biomater. 74B (2004) doi: /jbm.b [2]M. Takahashi, M. Kikuchi, Y. Takada, T. Okabe, O. Okuno, Electrochemical Behavior of Cast Ti-Ag Alloys, Dent. Mater. J. 25 (2006) doi: /dmj [3]M. Takahashi, M. Kikuchi, Y. Takada, O. Okuno, Corrosion Resistance of Dental Ti-Ag Alloys in NaCl Solution, Mater. Trans. 51 (2010) doi: /matertrans.m [4]M. Takahashi, M. Kikuchi, Y. Takada, Corrosion behavior of Ti-Ag alloys used in dentistry in lactic acid solution, Met. Mater. Int. 17 (2011) doi: /s y. 5]B.B. Zhang, Y.F. Zheng, Y. Liu, Effect of Ag on the corrosion behavior of Ti Ag alloys in artificial saliva solutions, Dent. Mater. 25 (2009) doi: /j.dental [6]B.B. Zhang, K.J. Qiu, B.L. Wang, L. Li, Y.F. Zheng, Surface Characterization and Cell Response of Binary Ti-Ag Alloys with CP Ti as Material Control, J. Mater. Sci. Technol. 28 (2012) doi: /s (12)

47 P-02 MESOPOROUS SILICA NANOPARTICLES FOR THE TREATMENT OF NAVITOCLAX RESISTANT TRIPLE NEGATIVE BREAST CANCER Autores: Gema Vivo Llorca 1,2,5, Alba García-Fernández 1,2,4,5, Danya Bandeira-Lima 5, Félix Sancenón 1,2,3, Ramón Martínez-Máñez 1,2,3,4 and Mar Orzáez 4,5 1. Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València. Spain. gevillo1@posgrado.upv.es 2. Departamento de Química, Universitat Politècnica de València, Camí de Vera s/n, 46022, València, Spain. 3. CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) Spain. 4. Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Valencia, Universitat Politècnica de València, 5. Centro de Investigación Príncipe Felipe, València, Spain. Centro de Investigación Príncipe Felipe, Laboratorio de Péptidos y Proteínas, Carrer d'eduardo Primo Yúfera, 3, València, Spain. Triple negative breast cancer, apoptosis, CRISPR/Cas9. Triple negative breast cancer (TNBC) represents 15% of breast carcinomas[1]. It is defined by the lack of the three main breast cancer biomarkers: oestrogen, progesterone and HER2 receptors[1]. Among the breast cancers, TNBC shows the poorest outcome and the highest lethality, due to its aggressiveness, the high risk of relapse and the rapid onset of metastasis[2]. Evasion of apoptosis is a hallmark of cancer, which usually results in chemotherapy resistance[3].tumors require evasion of cell death, reason why the antiapoptotic Bcl-2 family proteins is frequently overexpressed in breast cancers[3]. Being so, several inhibitors of Bcl-2 pro-survival family have been developed, such as ABT-737 or ABT-263 (navitoclax), targeting Blc-2, Bcl-xl, Bcl-w, but not MCL-1[1]. These inhibitors, also called BH3 mimetics, target and neutralize the pro-survival proteins, sensitizing cancer cells to death [1]. Overexpression of Mcl-1 causes resistance to these inhibitors, like ABT-263, in tumor cells[4]. Our goal is to overcome ABT-263 resistance in TNBC cells by combining MCL-1 gene editing, by CRISPR/Cas9 technology, with ABT-263 treatment. Combining both strategies in a multifunctional mesoporous silica nanoparticle (MSNP) has several advantages: (1) passive targeting to the tumor, due to the enhanced permeability and retention effect (EPR effect), which enables the preferential accumulation of MSNPs in the cancerous cells and (2) the simultaneous entry of both treatments in the same cell, which increases their effectiveness. In this paper, we present the first studies in which, after generating and characterizing a TNBC cell line resistant to ABT-263, we demonstrate its sensitivity to the co-treatment with MCL-1 inhibitors and navitoclax. We also characterize the toxicity and internalization of the MSNPs functionalized with polyethyleneimine (PEI) and coated with a CRISPR/Cas9 plasmid, demonstrating the potential of this type of nanoparticles as carriers for genome editing tools in TNBC. References [1] S. R. Oakes et al., Sensitization of BCL-2-expressing breast tumors to chemotherapy by the BH3 mimetic ABT-737, Proc. Natl. Acad. Sci., vol. 109, no. 8, pp , [2] G. Jerusalem, J. Collignon, H. Schroeder, and L. Lousberg, Triple-negative breast cancer: treatment challenges and solutions, Breast Cancer Targets Ther., p. 93, [3] M. M. Williams and R. S. Cook, Bcl-2 family proteins in breast development and cancer: could Mcl-1 targeting overcome therapeutic resistance?, Oncotarget, vol. 6, no. 6, pp , [4] J. Belmar and S. W. Fesik, Small molecule Mcl-1 inhibitors for the treatment of cancer, Pharmacol. Ther., vol. 145, pp ,

48 P-03 PSEUDOPEPTIDIC LIGANDS: SYNTHESIS AND Cu +2 RECOGNITION David Valverde, Belén Altava, M. Isabel Burguete, Santiago V. Luis Departamento de Química Inorgánica y Orgánica, Universidad Jaume I, Castellón, 12071, Spain. Ubicación permanente: ECEN-Vicerrectoría de Investigación, Universidad Estatal a Distancia, Costa Rica. dvalverdeb@uned.ac.cr keywords: Pesudopeptidic compounds, metal complexes, metal recognition. The preparation and study of organic ligands able to interact with specific transition metals are of great current interest for the relevant role of these metals in different areas of chemistry and biology [1]. Metal chelation is involved in many biological processes [2], and the inclusion of amino acid residues in the structure of ligands is an attractive strategy because this provides coordination environments of the metal ions similar to those found in living systems [3]. The combination of natural and non-natural components, properly designed using the knowledge gathered from natural systems, could achieve a desired functionality with low molecular weight molecules [4]. O NHHN O O R OSu + H 2 N NHCBz H 2 N i) ii) O H 2 N NHHN O R R NH 2 iii) iv) R NHHN R 1 R = CH 2 Ph 2 R = CH(CH3)2 3 R = CH 2 Ph 4 R = CH(CH3)2 Scheme 1. Synthesis of ligands 3 and 4. (i) DME, 20 h, r.t., 70 80%; (ii) HBr/AcOH 8 h, NaOH, r.t., 60 70%; (iii) benzaldehyde, methanol, 2 h, r.t; (iv) sodium borohydride, 18 h, r.t., 70 80% [1]. Regarding metal complexation, our group has studied the coordination ability of some C2 symmetrical bis(amino amides) derived from amino acids towards Cu(II) and Zn(II) ions [5]. In this work, we present the synthesis of bis(amino amide) ligands derived from L- valine and L-phenylalanine (figure 1), as well as the analysis of their binding ability towards different M(II) species. References [1] L. Gorla, V. Martí-Centelles, B. Altava, M.I. Burguete, S.V. Luis, Dalton Trans , [2] R.R. Crichton, D.T. Dexter, R.J. Ward, Coord. Chem. Rev. 2008, 252, [3] J. Dong, Y. Wang, Q. Xiang, X. Lv, W. Weng and Q. Zeng, Adv. Synth. Catal. 2013, 355, [4] S. V. Luis and I. Alfonso, Acc. Chem. Res. 2014, 47, [5] (a) S. Blasco, M. I. Burguete, M. P. Clares, E. García-España, J. Escorihuela, S. V. Luis. Inorg. Chem. 2010, 490, (b) I. Martí, A. Ferrer, J. Escorihuela, M. I. Burguete and S. V. Luis. Dalton Trans. 2012, 41, Acknowledgements. Financial supported by Universidad Estatal a Distancia, Costa Rica and MINECO (CTQ R), Generlitat Valenciana (Prometeo ). 48

49 P-04 REVERSIBLE RESPONSIVENESS HYDROGELATORS DERIVED FROM C2 PSEUDOPEPTIDES Adriana Valls 1, Belén Altava 1, María Isabel Burguete 1, Santiago Vicente Luis 1 1 Departamento de Química Inorgánica y Orgánica, Universitat Jaume I, Castellón, España avalls@uji.es, estevef@uji.es Keywords: hydrogelator, biocompatibility, pseudopeptides The design and synthesis of gelators is a topic of current interest in supramolecular chemistry due to their broad reported applications [1, 2, 3]. Essential properties of the gels, embrace their ability to be formed in a variety of solvents, the need of gelation processes occurs in a minimum concentration of the gelator and their stability behavior under different external stimuli [4], their biocompatibility [5] or their capacity to develop structured porosity [1]. In this context, a new family of open chain-pseudopeptidic compounds (Figure 1) in which the aliphatic spacer has been functionalized with a pendant carboxylic group have been prepared with excellent yields. These compounds exhibit a high level of molecular diversity providing not only strong hydrogelation properties, but also the capacity to form hydrogels under external stimuli. The influence of the amino acid side chain, in their gelling properties has been investigated [6]. O R O N H NHCbz O N O H N H O R NHCbz R = CH(CH 3)2 R = CH(CH 2 CH 3)CH 3 R = CH 2 Ph Fig. 1: General structure of C 2 open chain pseudopeptides [1] B. Escuder, F. Rodríguez-Llansola, J.F.Miravet, New J. Chem., 2010, 34, [2] I. Tokarev, S. Minko Adv. Mater., 2010, 22, [3] M.E. García, S. Pagola, A. Navarro Vázquez, D.D. Phillips, C. Gayathri, H. Krakauer, P.W. Stephens, V.E. Nicotra, R.R. Gil, Angew. Chem., 2009, 48, [4]S. Varghese, N.S.S. Kumar, A. Krishna, D.S.S. Rao, S.K. Prasad, S. Das, Adv. Funct. Mater., 2009, 19, [5] A. R. Hirst, B. Escuder, J. F. Miravet, D. K. Smith, Angew. Chem., 2008, 47, [6] J. Becerril, M.I. Burguete, B. Escuder, F. Galindo, R. Gavara, J.F. Miravet, S.V. Luis, G. Peris, Chem. Eur. J., 2004, 10,

50 P-05 MESOPOROUS SILICA NANODEVICES FOR GENE SILENCING Amelia Ultimo 1, Mar Orzaez 2,3, Ramón Martínez Máñez 1,3,4, Eduardo Ruiz Hernández 5,6 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM) Universitat Politècnica de València - Universitat de València, Camí de Vera s/n, 46022, Valencia (Spain), amul1@doctor.upv.es. 2 Centro de Investigación Príncipe Felipe, C/ Eduardo Primo Yúfera 3, 46012, Valencia (Spain). 3 Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Valencia, Universitat Politècnica de València, Centro de Investigación Príncipe Felipe, Valencia (Spain). 4 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). 5 School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin (TCD), Dublin 2, Ireland. 6 Trinity Biomedical Sciences Institute, TCD, Dublin 2, Ireland. Keywords: mesoporous silica nanoparticles, sirna delivery, degenerative eye diseases The main objectives of the present work are to design and to test mesoporous silica nanoparticles (MSNs) as nanocarriers for sirna transport and delivery. In particular, this gene silencing strategy has been addressed to molecules involved in retinal neovascularization, a key process in several irreversible and degenerative eye diseases, among them age-related macular degeneration (AMD). The efficient cellular delivery of therapeutic sirna is a critical step, so that highly efficient sirna carriers are required for stable RNA transport, cellular uptake and intracellular RNA release [1]. Therefore, functionalized nanodevices based on MCM-41-like nanoparticles loaded with sirna and externally coated with polyethyleneimine (PEI) have been synthesized and characterized by transmission electron microscopy (TEM), porosimetry and dynamic light scattering (DLS). As retinal pigment epithelium (RPE) is indispensable for the visual process conducted by photoreceptors and has been found to be a key component in AMD initiation and progression [2], the human retinal pigment epithelial cell line ARPE19 was selected for the in vitro evaluation of the developed system. Cells viability, nanoparticles uptake and performance were studied using this model. Silencing efficacy was observed by immunoblot. References [1] A. Egorova, A. Shubina, D. Sokolov, S. Selkov, V. Baranov, A. Kiselev. International Journal of Pharmaceutics. 2016, 515, [2] Y. Wang, D. Shen, V. M. Wang, C-R. Yu, R-X. Wang, J. Tuo, C-C. Chan. Apoptosis. 2012, 17(11),

51 P-06 WORKING NANOMATERIALS: IS PRINCIPLE OF CAUTION ENOUGH? Francisco Torrens 1, Gloria Castellano 2 1 Institut Universitari de Ciència Molecular, Universitat de València, Edifici d Instituts de Paterna, P. O. Box 22085, València, Spain, torrens@uv.es 2 Departamento de CC. Experimentales y Matemáticas, Facultad de Veterinaria y CC. Experimentales, Universidad Católica de Valencia San Vicente Mártir, Guillem de Castro- 94, València, Spain Keywords: opposed image, medieval stained-glass window, noble metal nanoparticle Consider a pair of opposed images. On one hand, a medieval stained-glass window where Au and Ag nanoparticles were used, empirically, to colour and in order that this were different seen from inside or outside. The counter-image represents a modern industry of paints based on nanoparticles [1]. Many nanomaterials share nature and atomic composition but present different properties [2]. In their handling, Principle of caution should be applied: Nanoparticles should be considered as much toxic as their corresponding bulk materials [3]. However, is it enough [4]? The used method is based on: (1) contrast of opposed images; (2) avoiding topics; (3) combination of historical with present studies on a techno-scientific subject, with a common purpose of taking part politically in ethico-political futures [5]. The meaning of the first image is commented on via discussion of other dualities: Representation that manifests subjacent mirror symmetry in atomic physics and other mirror asymmetry. We put yellow and red that are industry warning colours! Representation of a landscape without atoms and other with atoms. Science relationship with power in history (religion) and nowadays. Mendeleev s periodic table of the elements and a modern view like a game sink the fleet. Mendeleev s periodic table of the holes and scientists periodic table. Letter signed by Einstein to President Roosevelt and logo of Atoms for Peace Scientists that worked in Manhattan Project and exit poster of the installations of Oak Ridge of Manhattan Project by James. E. Westcott. Campaign Close down Cofrentes and round table Nuclear Weapons Radiography. Travelling show Atoms for Peace (1958 ), presenting nuclear energy s peaceful applications, and American nuclear cover-up in Spain in Palomares disaster (1966). Acknowledgements. We thank St. Mary Basilica (Daroca), and support from Generalitat Valenciana (Project No. PROMETEO/2016/094) and Universidad Católica de Valencia San Vicente Mártir (Project No. UCV.PRO AIV.03). References [1] F. Torrens, G. Castellano. Certamen Integral de la Prevención y el Bienestar Laboral, València, 2016 [2] F. Torrens, G. Castellano. Methodologies and applications for analytical and physical chemistry. Apple Academic CRC, Waretown, NJ. In press [3] F. Torrens, G. Castellano. Tecnología, ciencia y sociedad. Global Knowledge Academics, València. In press [4] F. Torrens-Zaragozá, G. Castellano-Estornell. Intersanitario. ImpulSalud, València. In press [5] F. Torrens, G. Castelano. Congrés Ciència, Política, Activisme i Ciutadania, València, Accepted 51

52 P-07 Sistema de posicionamiento automático de muestras en el equipo de experimentación de hipertermia óptica José Manuel Terrés 1, Rafael Masot 1, Miguel Penadés 1, David Cascales 1, Javier Ibáñez 1 1 Instituto de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, joterha@upv.es Palabras clave: Hipertermia óptica, Posicionamiento automático, Experimentación La hipertermia mediante la excitación de nanopartículas es una técnica actualmente en desarrollo, de la cual se espera hallar una forma, complementaria y más eficiente, de tratar neoplasias, tanto por la acción directa de la elevada temperatura, como mediante la liberación de fármacos de forma localizada, gracias a la funcionalización de las nanopartículas. [1] Para excitar las nanopartículas es necesario que éstas sean irradiadas por una luz de una longitud de onda determinada. Para ello, se desarrolló un equipo capaz de controlar la intensidad de un haz láser y se demostró que era capaz de aumentar la temperatura en el medio en el que se suspenden las nanopartículas; este equipo y el resultado del experimento se muestran en la Figura 1. [2] Figura 1. Equipo de control del láser y calentamiento de las nanopartículas al ser irradiadas por éste. Durante la experimentación han surgido nuevas necesidades, una de ellas es el posicionamiento de muestras automático, de tal forma que los investigadores no tengan que situar la muestra bajo el haz manualmente, lo que conlleva alterar las condiciones del experimento como el tiempo entre irradiaciones y temperatura ambiente. Para dar solución a esta necesidad, se ha desarrollado un sistema electromecánico consistente en una estructura móvil con dos motores paso a paso. Estos motores se controlan a través de una placa de circuito impreso que integra un microcontrolador de 32 bits, y que se comunica a través de puerto serie con una interfaz gráfica donde el usuario puede seleccionar la posición que se desea irradiar. Referencias [1] H. Norouzi, K. Khoshgard, F. Akbarzadeh. Lasers in Medical Science. 2018, pp [2] R. Montes, A. Hernández, J. Ibáñez. Sensors and Actuators, A: Physical. 2017, 255,

53 P-08 SÍNTESIS, CARACTERIZACIÓN Y ENSAYO DE MATERIALES INHIBIDORES DE POLIFENOL OXIDASA Juan Antonio Soler 1, Sara Muñoz 2, Tania Godoy 4, Jamal el Haskouri 3, Pedro Amorós 3, Ana Costero 4,5, Margarita Parra 4,5, Ángel Argüelles 2, Ana Andrés 2, Jose Vicente Ros- Lis 1,5 1 Departamento de Química Inorgánica, Universitat de València, sojuanan@alumni.uv.es 2 Instituto de Ingeniería de Alimentos para el Desarrollo, Universitat Politècnica de València 3 Instituto de Ciencia de Materiales, Universitat de València 4 Instituto de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat de València 5 Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina, Palabras clave: inhibición, polifenol oxidasa y materiales híbridos. El estudio de la inhibición de la polifenol oxidasa (PPO) tiene como finalidad evitar el pardeamiento enzimático en frutas y verduras. Este efecto negativo es una de las principales pérdidas de calidad en vegetales. El pardeamiento enzimático se produce por reacciones de oxidación catalizadas por la PPO, especialmente en aquellos productos ricos en polifenoles. En concreto, esta enzima actúa en dos tipos de sustrato (actividad creolasa en monofenoles y catecolasa en o-difenoles), en ambos casos el sustrato es oxidado a o- cresol y polimeriza formando pigmentos pardos marrones. Figura 1. Figura 1.- Oxidación de monofenol y o-difenol a o-quinona por la acción de la enzima PPO. Clásicamente se han empleado los sulfitos para la inhibición de la PPO, produciendo alergias en la población, por lo que se ha restringido su uso en bebidas y alimentos. Frente a ellos, los nanomateriales pueden ser una alternativa interesante para evitar el pardeamiento enzimático. Permiten inhibir la actividad de la PPO de forma heterogénea, con lo que separar el inhibidor del alimento es más sencillo. En el presente trabajo se han ensayado soportes mesoporosos de sílice, en concreto del tipo UVM-7 y de tipo Stober. Para dotarlos de actividad, se han funcionalizado con inhibidores de PPO modificados para la inclusión de grupos silano. Por último, se realizarán ensayos para medir la actividad enzimática en presencia de estos materiales híbridos. Referencias [1] Cabrera, S., El Haskouri, J., Guillem, C., Latorre, J., Beltrán-Porter, A., Beltrán-Porter, D., Marcos, M. D. y Amorós, P. (2000). Generalised synthesis of ordered mesoporous oxides: the atrane route. Solid State Sciences, 2 (2000), [2] Morante, J., Agnieszka, A., Bru-Martinez, R., Carranza, M., Pico-Saltos, R, y Nieto, E. (2014). Distribución, localización e inhibidores de la polifenol oxidasa en frutos y vegetales usados como alimento. Ciencia y Tecnología, 7 (1),

54 P-09 INTERACTION STUDY BETWEEN PPO AND MESOPOROUS SILICA NANOPARTICLES WITH DIVERSE FUNCTIONALIZATION Sara Muñoz-Pina 1, José V. Ros-Lis 2, Ángel Argüelles 1, Ramón Martínez-Máñez 3,4, Ana Andrés 1 1 Instituto Universitario de Ingeniería de Alimentos para el Desarrollo (IUIAD-UPV). Universitat Politècnica de València Camino de Vera s/n, 46022, Valencia, Spain. samuopi@upvnet.upv.es 2 Inorganic Chemistry Department, Universitat de València , Burjassot, Valencia Spain. 3 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico. Universitat Politècnica de València - Universitat de València. Camino de Vera s/n, 46022, Valencia, Spain. 4 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). 3 keywords: PPO, immobilization, UVM-7 The inhibition or isolation of the enzyme polyphenol oxidase (PPO) present in most fruits and vegetables is of great interest for the food industry, as it catalyses the oxidation of polyphenols in compounds known as melanoids causing an undesirable colour change on the surface of these foods. [1] This reaction is known as enzymatic browning and, although the industry is already relieving its effects with some processes, they are still looking for a more effective alternative and with fewer adverse effects. In recent years there has been a great advance in the field of nanotechnology and it has been proved their great compatibility with proteins and enzymes. [2] For that reason the use of nanoparticles in order to palliate the enzymatic browning may be an interesting tool as an alternative to current processes. Thus, the aim of this work has been to study the interactions between the PPO enzyme and the different surface of UVM-7 in order to investigate whether any of the chemical groups attached to the UVM-7 is capable of immobilize the enzyme and if they affects to it enzymatic activity. The results showed how some of these functionalisation can serve as immobilizers of the polyphenol oxidase allowing its totally elimination from the medium. References [1] J. B. Gutierrez. Ciencia Bromatológica: principios generales de los alimentos. 1st ed, Díaz de Santos, España [2] A. A. Shemetov, I. Nabiev, A. Sukhanova. ACS Nano. 2012, vol

55 P-10 DESARROLLO DE UN BIOSENSOR LABEL-FREE BASADO EN ESPECTROSCOPIA DE INTERFERENCIA POR REFLEXIÓN Gabriel Sancho-Fornes 1, Miquel Avella-Oliver 1, Javier Carrascosa 1, Rosa Puchades 1,2, Ángel Maquieira 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnilógico (IDM) Universitat Politècnica de València-Universitat de València, Camino de vera s/n, Valencia, gabsanfo@upvnet.upv.es 2 Departamento de Química, Universitat Politècnica de València, Camino de vera s/n, Valencia. biosensor, interferometría, sulfasalazina La interferencia por reflexión es una propiedad óptica muy adecuada para su aplicación en biosensado, principalmente por la robustez y simplicidad del sistema de detección [1,2]. En este trabajo se optimizó y desarrolló una estructura interferométrica destructiva, que consiste en una capa de Au de 5 nm sobre 65 nm de ZnS, 15 nm de una aleación de Ag, In, Sb y Te, 60 nm de ZnS y 50 nm de Au; y otra constructiva en la que el grosor de las dos capas de ZnS es de 85 nm y el de la aleación de 20 nm. Las capas se depositaron una a una mediante sputtering sobre policarbonato. Las medidas se basaron en las variaciones del haz reflejado y se realizaron con un sistema basado en una fuente laser y un fotodetector que resulta muy robusto con prestaciones muy competitivas y coste reducido. El oro mostró una gran capacidad de adsorción de biomoléculas que actúan como sondas, proporcionando selectividad y mejorando la sensibilidad. Las prestaciones de las dos estructuras interferométricas fueron comparadas, demostrando que además de para macromoléculas, estos sustratos pueden aplicarse para la determinación de moléculas de bajo peso molecular como fármacos. Para ello, se ha puesto a punto un immunoensayo competitivo para la determinación de sulfasalazina sin marcaje, alcanzando un límite de detección de 11 y 81 ng ml -1 con las estructuras interferométricas destructiva y constructiva, respectivamente. Estos resultados apuntan además a su aplicabilidad en otros ámbitos como el medioambiente y alimentación. Interferometria destructiva Interferometria constructiva Intensidad Intensidad Figura 1. Esquema del biosensor basado Longitud de onda Longitud de onda en espectroscopia de interferencia por reflexión. pteno Analito Anticuerpo Agradecimientos Este trabajo ha sido financiado por el Ministerio Español de Economía y Competitividad (CTQ R), FEDER, y GVA (PROMETEO II/2014/040). Referencias [1] S. Rau, G. Gauglitz. Analytical and Bioanalitical Chemistry. 2012, 402 (1), [2] A. Kussrow, C.S. Enders, D.J. Bornhop. Analytical Chemistry. 2012, 84 (2),

56 P-11 BACTERIAL ENANTIORECOGNITION OF KETOPROFEN: READY BIODEGRADABILITY TEST María José Medina-Hernández 1, Laura Escuder-Gilabert 1, Yolanda Martín-Biosca 1, Emilio Bonet-Domingo 2, Mireia Pérez-Baeza 1, Salvador Sagrado 1,3 1 Departamento de Química Analítica, Universitat de València, Burjassot, Valencia, Spain. sagrado@uv.es 2 GAMASER S.L., C/ Isaac Peral, 4, Parque Tecnológico, Paterna, Valencia, Spain. 3 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM). Universitat Politècnica de València, Universitat de València. Avd. Vicente A. Estellés s/n, Burjassot, Valencia, Spain. Keywords: bacterial enantiorecognition, biodegradation profile, emergent contaminants Enantiomers of chiral pharmaceuticals are becoming of environmental concern because of their different behavior [1]. Ketoprofen, marketed as racemate and as S-(+) enantiomer, is a nonsteroidal anti-inflammatory drug widely used. Thus, it could cause unpredictable enantioselective health and environmental problems, if it is not efficiently removed in wastewater treatment plants (WWTPs). The present communication reports a enantiobiodegradability batch mode test of enantiomers of ketoprofen using minimal salts medium inoculated with activated sludge collected from a Valencian WWTP. The ketoprofen concentrations have been monitored by means of a HPLC method using Chiralart Cellulose-SC (celulose tris(3,5-dichlorophenylcarbamate) as analytical column and UV detection. The Biodegradation in percentage (BD, %) of biotic assays for the racemate, along the incubation time was estimated (Fig. 1). For ketoprofen, nonenantioselective biodegradation was observed in the current experimental conditions assayed. Fig.1. Ketoprofen enantiomers BD (%): experimental (o & ) and modelled (-) Acknowledgements This work has been supported by the Project CTQ R (MINECO/FEDER, UE). References [1] A.R. Ribeiro, P.M.L. Castro, M.E. Tiritan. Environmental Chemistry for a sustainable world. Springer-Verlag, Berlin

57 P-12 BACTERIAL ENANTIORECOGNITION OF EMERGENT CONTAMINANTS: PEAK AREA-BASED BIODEGRADABILITY MODELS María José Medina-Hernández 1, Laura Escuder-Gilabert 1, Yolanda Martín-Biosca 1, Emilio Bonet-Domingo 2, Mireia Pérez-Baeza 1, Salvador Sagrado 1,3 1 Departamento de Química Analítica, Universitat de València, Burjassot, Valencia, Spain. sagrado@uv.es 2 GAMASER S.L., C/ Isaac Peral, 4, Parque Tecnológico, Paterna, Valencia, Spain. 3 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM). Universitat Politècnica de València, Universitat de València. Avd. Vicente A. Estellés s/n, Burjassot, Valencia, Spain. Keywords: bacterial enantiorecognition, biodegradation profile, emergent contaminants Chiral pharmaceuticals are becoming of environmental concern because of the different behavior between enantiomers [1]. Biodegradability tests allow a first evaluation for environmental risk assessment (e.g., biodegradation curves BD vs. time). Usually BD data come from calculations and models based on the concentration-time curves (so, previous calibration curve are required). The present communication reports BD and enantiomeric fraction (EF) novel equations and models using, for the first time, peaks areas as dependent variables; thus avoiding the need of using calibration data and their corresponding errors/uncertainties that affects the quality of the results. Ibuprofen is used as a model example. A ready biodegradability test is conducted using an activated sludge as inoculum and racemic mixtures of ibuprofen. The measured chromatographic peak areas of the enantiomers and their corresponding BD-t data and curves are calculated (Fig. 1). Fig.1. Ibuprofen enantiomers experimental peak areas (A) and their corresponding BD-t data and curves (B) Acknowledgements This work has been supported by the Project CTQ R (MINECO/FEDER, UE). References [1] K. Kummerer. Pharmaceuticals in the Environment. Springer-Verlag, Berlin,

58 P-13 Theoretical and computational study of the affinity of cardiac versus skeletal troponin I towards cardiac troponin I antibody Jad Sabek, Paula Martínez Pérez, Jaime García-Rupérez Nanophotonics Technology Center, Universitat Politècnica de València, Camí de Vera s/n, Valencia, 46022, Spain 3 keywords: (Cardiac Troponin I, Binding sites, Molecular docking) Cardiac troponin I (ctni) is currently the gold-standard biomarker for the fast and early detection of a myocardial failure. In this context, we report a computational study of the interaction between ctni and its specific antibody (αctni). Furthermore, we also report a comparative study of the binding efficiency of the ctni antibody with the ctni and with its principal interferon, the skeletal troponin I (stni). The objective of this work is demonstrating that binding and selectivity studies can be carried out computationally, what may be very relevant for the development of biosensing devices with a better recognition performance and with a lower cross-reactivity. The computational study was performed using different simulation platforms. FTSite and FTMap were used for the determination and mapping of the binding sites [1]. Then, FTDock and pydock were used to study molecular docking [2]. Finally, molecular dynamics (MD) was analyzed using GROMACS [3]. This study can also be applied to a wide range of different scenarios were other targets (e.g., lipids, oligonucleotides, etc.) or other applications (e.g., pharmacological drug design) are considered. Figure 1: Binding sequences of ctni (A) and stni (B) towards the Fab region of the ctni antibody. References Table 1: Comparison of the binding energies between ctni and stni towards the Fab region of the ctni antibody. Complex Ele. Desolv VDW Total. stni-αctni ctni-αctni Ele. : Electrostatic energy Desolv. : Desolvation energy VDW: Van Der Waals energy [1] Y. Yuan, J. Pei, L. Lai. Curr. Pharm. Des. 2016, 19 (12), [2] M. Cheng, L. Blundell, J. Fernandez-Recio. Proteins. 2007, 68, [3] H. Berk, C. Kutzner, D. Van der Spoel, E. Lindahl. J. Chem. Theory Comput. 2008, 4 (3),

59 P-14 A comparative study between two tripodal halogen- and hydrogen bonding receptors for anion sensing Paula Sabater, Fabiola Zapata, Antonio Caballero*, Pedro Molina* University of Murcia, Departamento de Química Orgánica, Campus de Espinardo, E Murcia, Spain, paula.sabater@um.es 3 keywords: anion recognition, halogen bonding, imidazolium Anion recognition has become one of the most important research field in supramolecular chemistry due to the importance of anions in chemistry, biology and environmental processes.[1] In this way, the utilization of different non-covalent interactions Anion Receptor has attracted a great interest, being the Halogen Boding interaction one of the most promising interaction to employ in anion recognition in the last years.[2] We report here the synteshis and comparative study of the anion sesing propierties of a new halogen bonding tris-bromoimidazolium receptor PF 6 - (Figure 1a) with the analogous hydrogen bonding one PF 6 - (Figure 1b). The evaluation of the sensing properties was realized by fluorescence, UV-V and NMR spectroscopy towards the following set of anions as tetrabutylammonium salt: HP 2 O 7 3, H 2 PO 4, SO 4 2, HSO 4, NO 3, F, Cl, Br, I, AcO, ClO 4, BF 4 and C 6 H 5 CO 2. The addition of HP 2 O 3 7, H 2 PO 4, SO 2 4, F, AcO and C 6 H 5 CO 2 anions to a solution of the receptors PF - 6 and PF - 6 promoted significant changes in their emission bands, while the others anions tested had no effect on the emission spectrum. Whereas the addition of those anions to a solution of the receptor PF - 6 cause the quenching of the monomer emission bands in all cases, the addition of H 2 PO 4 anions to the receptor PF - 6 promotes a progressive increasing of a new broad emission band at λ = 469 nm, attributed to the anthracene excimer fluorescence, and the concomitant decreasing of the monomer emission bands at λ = 398 and 419 nm presents in 3 the free receptor. The addition of HP 2 O 7,SO 2 4, F, AcO and C 6 H 5 CO 2 anions to a solution of receptor PF - 6 promoted the quenching of the monomer emission bands just as in the receptor PF - 6. We acknowledge to the Ministerio de Economía y Competitividad of Spain and FEDER projects CTQ P and CTQ P, and Fundación Séneca Región de Murcia (CARM) projects (18948/JLI/13 and 19337/PI/14). P. Sabater also acknowledges to the University of Murcia for a predoctoral grant. References [1] P. Molina, F. Zapata, A. Caballero, Chem. Rev., 2017, 117, [2] L.C. Gilday, S.W. Robinson, T.A. Barendt, M.J. Langton, B.R. Mullaney, P.D. Beer, Chem. Rev., 2015, 115,

60 P-15 ESTUDIO DE LA COMPARATIVA HORNO CONVENCIONAL VS HORNO MICROONDAS EN LA ELABORACIÓN DE FLAN DE LIMÓN UTILIZANDO TECNOLOGÍAS DE IMÁGEN TÉRMICA. Susana Rubio-Arraez 1, Diego Alcañiz 1, Ruth de los Reyes 1, Maria Luisa Castelló 1, Maria Dolores Ortolá 1. 1 Intituto Universitario de Ingeniería de Alimentos para el Desarrollo. Universitat Politècnica de València, Camino de Vera s/n 46022, Valencia, Spain. Autor de correspondencia: suruar@upvnet.upv.es 3 palabras clave: Termografía, Hornos microondas y convencional, flan de limón El objetivo principal del presente estudio fue realizar un seguimiento y comparación de procesos de horneado (en horno convencional o en horno microondas) en la elaboración de flanes de limón aplicando tecnologías de imágenes térmicas mediante una cámara termográfica. Al mismo tiempo, se han realizado las determinaciones de las propiedades colorimétricas y mecánicas sobre el producto terminado. El interés de la industria alimentaria por ofrecer productos de alta calidad nutricional y con un aporte de beneficios saludables se une a la demanda del consumidor por productos con buen sabor y aspecto atractivo [1]. Sin embargo, al incorporar zumo de fruta natural como es el caso del limón, a productos como el flan, lo enriquece en gran medida por la cantidad de propiedades que le aporta y a su vez, es una forma de darle salida en el mercado al excedente de producción de cítricos. No obstante, la elaboración de postres como el flan de limón, que tradicionalmente se elabora en un horno convencional, podría optimizarse con la implementación de hornos microondas que permiten acortar los tiempos de horneado, y además es una energía ecológica [2,3]. Teniendo en cuenta los resultados obtenidos podemos concluir, que mediante la detección del sensor de la cámara termográfica se ha podido observar un calentamiento más homogéneo durante el proceso de horneado convencional, mientras que en el de microondas hubo diferencias marcadas, concentrándose el calentamiento, en puntos donde existía fase acuosa. En concordancia con los parámetros colorimétricos obtenidos, luminosidad y tono, las muestras de flan de limón elaboradas en el horno convencional muestran valores superiores frente a los obtenidos para las muestras elaboradas en el horno microondas. Sin embargo, al realizar el análisis de perfil de textura (TPA) hemos observado que los parámetros dureza y gomosidad son superiores en las muestras elaboradas en el horno microondas. Para concluir, este sistema ha sido aplicado con éxito en el proceso. Referencias [1] N. Candia-Muñoz, M. Ramirez-Bunster, Y. Vargas-Hernández, L. Gaete-Garretón. Physics Procedia. 2015, 70, [2] W. Liu, T.C. Lanier. Food Research International. 2016, 81, [3] S. Chandrasekaran, S. Ramanathan, T. Basak. Food Research International. 2013, 52,

61 P-16 NOVEL NIR-PHOTOACTIVE NANOPLATFORM FOR PDT: 2 CAPPED WITH PORPHYRIN Ignacio Rosa-Pardo 1, Mykhaylo S. Petrov 1, Néstor Estebanez 1, Daniel Caminos 1,2, María González-Béjar 1, Raquel E. Galian 1, and Julia Perez-Prieto 1. 1 Instituto de Ciencia Molecular (ICMOL), Univ. de Valencia, Catedrático José Beltrán 2, 46980, Paterna, Valencia, Spain. igropar@alumni.uv.es 2 INFIQC (UNC-CONICET) Dpto. de Química Orgánica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, X5000HUA, Córdoba, Argentina Keywords: (mesoporous silica, upconversion luminescence, photodynamic therapy The integration of different functional nanomaterials to prepare a platform with relevant features and applications is a subject of increasing interest. Recently, our group has developed a nanoplatform (NPT), specifically Fe 3 O where MSN means mesoporous silica, and decorated it with Rose Bengal as photosensitizer for photodynamic therapy (PDT). This NPT generates singlet oxygen efficiently upon excitation with visible light [1]. Here we report a novel biocompatible NPT composed of four types of materials with different functionalities: magnetic nanoparticles (Fe 3 O 4, MNP) and core-shell upconversion nanoparticles (UCNP) incorporated into the mesoporous silica (m-sio 2 ) to provide the NPT biocompatibility as well as NIR-induced luminescence and magnetic properties. The synthesis of the individual nanoparticles was carried out following reported methodologies [2,3]. The key step was the homogeneous encapsulation of both kind of nanoparticles to generate the MNP-UCNP@MSN nanoplatform. Different techniques were used to characterize the system, such as UV-visible and FTIR spectroscopy, microscopy, magnetism and thermogravimetry. Finally, the NPT was decorated with a porphyrin, PP, (Figure 1). The photophysical properties of the nanohybrid and the efficiency to generate singlet oxygen will be discussed. References [1] Rosa-Pardo, I.; Roig-Pons, M.; Heredia, A. A.; Usagre, J. V.; Ribera, A.; Galian, R. E.; Perez-Prieto, J.,. Nanoscale 2017, 9 (29), [2] WangWang; Luo, J.; Fan, Q.; Suzuki, M.; Suzuki, I. S.; Engelhard, M. H.; Lin, Y.; Kim, N.; Wang, J. Q.; Zhong, C.-J.,. J. Phys. Chem. B 2005, 109 (46), [3] Ren, W.; Tian, G.; Jian, S.; Gu, Z.; Zhou, L.; Yan, L.; Jin, S.; Yin, W.; Zhao, Y.,.RSC Advances 2012, 2 (18),

62 P-17 A novel marker for cellular senescence detection Sara Rojas 1,3, María Alfonso 1,3, Mª Salomé Sirerol 2, Isabel Fariñas 2, Ana Costero 1,3, Ramón Martínez 1,3. 1 Instituto Interuniversitario de Investigación, Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camí de Vera s/n Valencia, sarovaz@upvnet.uv.es 2 Unidad de Neurobiología Molecular. Universidad de Valencia Burjassot, Valencia 3 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BNN). 3 keywords: Cellular senescence, NSCs, α-fucosidase. Cellular senescence is a state of permanent arrest of the cell cycle that cells undergo after certain number of divisions or when they suffer stress or DNA damage. The accumulation of senescent cells with aging has been related to age-associated pathologies, including neurodegenerative disorders [1]. It is reported that neural stem cells (NSCs) progressively loss their neurogenic potential with aging. However, if this is caused by a senescence process still remains unknown [2]. In this scenario, a comprehensive understanding about senescence and its influence in the subependymal zone (SEZ) neurogenic niche is needed. To achieve this proposal, the reliable detection of senescent cells becomes a priority. The most widely used method to monitor senescence is the detection of the lysosomal activity of Senescence Associated β-galactosidase (SA-β-Gal), an enzyme that becomes especially abundant in senescent cell. However, this marker can fail to properly differentiate between senescent and no-senescent cells and, therefore, it has been proposed that the detection of another enzyme, a lysosomal hydrolase called α- fucosidase (α-fuc), could be a promising alternative. This is a novel biomarker with the potential to become a sensitive and specific marker in general and specially when SA-β- Gal does not provide a reliable signal [3]. The α-fuc enzyme activity is specifically increased in senescent cells. This can be observed through a histochemical labeling which uses X-Fuc as substrate, rendering an intensive blue staining [3]. This has enabled us to detect cellular senescence in vitro in senescence-induced cells and tissue samples from aging-mice models. This could offer us the possibility of identify those areas in the neurogenic niche where senescent cells are accumulated. References [1] D. Muñoz-Espín, M. Serrano. Nature Rev Mol Cell Biol. 2014, 15, 482. [2] J.C. Acosta, A. Banito, T. Wuestefel, A. Georgilis et al. Nature Cell Biol. 2013, 15, [3] D.G. Hodelbrand, S. Lehle, A. Borst, S. Haferkamp et al. Cell Cycle. 2013, 12,

63 P-18 Integrated Network Sensors for Smart Corrosion Monitoring of Reinforced Concrete Structures J.E. Ramón 1, J.M. Gandía-Romero 1, R. Bataller 1, K. Díaz 2 and M. Valcuende 3. 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera, s/n Valencia, Spain. 2 Escuela Técnica Superior de Ingeniería de Edificación, Universitat Politècnica de València, Camino de Vera, s/n Valencia, Spain. 3 Departamento de Construcciones Arquitectónicas, Universitat Politècnica de València, Camino de Vera, s/n Valencia, Spain. Registered author joramza@arqt.upv.es Keywords: sensor, corrosion, structure The use of embedded sensors to monitor the state of reinforced concrete structures (RCS) has been gaining increasing interest in the last few decades [1]. The information provided by these sensors allows to detect in advance emerging corrosion processes, which improves structural safety and entails savings in inspection and maintenance. The Integrated Network Sensors for Smart Corrosion Monitoring (INESSCOM) is an innovative system developed to real-time corrosion monitoring of RCS at different locations [2]. This system has been patented [3] and it stands out by its capacity to carry out the acquisition, storage and transmission of data entirely autonomously. INESSCOM has been installed in a RCS prototype to continuously monitor the state of the reinforcements in a large number of control points. Several results obtained in this real application are presented here. Figure 1 shows the corrosion current density (i CORR ) graph obtained where chloride contaminated zones can be easily identified. The system was also able to record an increasing corrosion trend in those areas where cracks appeared unexpectedly during the concrete curing and hardening processes. Therefore, INESSCOM has demonstrated to be an effective system to monitor the durability of RCS. Figure 1. Corrosion current density (i CORR ) monitoring of three parts of the structure wall. References [1] C. Andrade, I. Martínez, C. Alonso and J. Fullea, Materiales de Construcción, 2001, vol.51, [2] J.E. Ramón, J.M. Gandía-Romero, M. Valcuende and R. Bataller, Vitruvio, 2016, vol.1, no.1, [3] M. Alcañiz, R. Bataller, J.M. Gandía-Romero, J.E. Ramón, J. Soto and M. O. Valcuende, Sensor, red de sensores, método y programa informático para determinar la corrosión en una estructura de hormigón armado, International patent, Publication number: WO A1, Nov

64 P-19 DEVELOPMENT OF GATED MATERIALS TO DETECT PATHOGENS Luis Pla 1,2,3, Sara Santiago-Felipe 1,2,3, Àngela Ribes 1,2,3, Elena Aznar 1,2,3, M. Angeles Tormo-Mas 4, Javier Pemán 4, Félix Sancenón 1,2,3, Ramón Martínez-Máñez 1,2,3. 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, València (Spain), plablas@upv.es 2 CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN) 3 Unidad Mixta de Investigación en Nanomedicina y Sensores, Universitat Politècnica de València, Instituto de Investigación Sanitaria La Fe, València (Spain). 4 Grupo acreditado de investigación Infección Grave, IIS La Fe, Avenida Fernando Abril Martorell, 126, Valencia, Spain. 3 keywords: Pathogens, molecular gates, mesoporous Current methodologies for clinical detection of pathogens are based in body fluid culture to grow possible present microorganisms, followed by identification of the specific pathogen. This procedure may take up several days, or even a week. Thus, it is imperious to develop new detection systems for a rapid detection of pathogens in an easy and reliable way. [1,2] Herein we present two different sensing approaches for the detection of two types of pathogens, a bacteria and a fungus, based on hybrid organic-inorganic porous materials. Recognition is undertaken through molecular gates, [3] specific biomolecular structures which can be constituted by antigens/antibodies, enzymes, nucleic acids/dna, etc. and are able to control the release of an entrapped reporter. Optical signaling in response to the presence of the target pathogen is accomplished by monitoring the fluorescence of the released dye from the pore voids to an aqueous solution. Preliminary results are encouraging and confirm their potential use as sensing probes in hospital microbiology departments. References [1] S. Chatterjee, S.V. Alampalli, R.K. Nageshan, S.T. Chettiar, S. Joshi, U. Tatu, BMC Genomics 2015, 16, [2] E.E. Alahi, S.C. Mukhopadhyay, Sensors 2017, 17, [3] E. Aznar, M. Oroval, Ll. Pascual, J.R. Murguía, R. Martínez-Máñez, F. Sancenón, Chem. Rev. 2016, 2,

65 P-20 FLUORINATED PSEUDOPEPTIDIC CAGES Edgar Peris, María Catalá, M. Isabel Burguete and Santiago V. Luis Grupo de Química Sostenible y Supramolecular, Departamento de Química Inorgánica y Orgánica, Universitat Jaume I, 12071, Castellón, Spain, esalom@uji.es keywords: molecular recognition, fluorine groups, pseudopeptidic cages Simple open chain and macrocyclic pseudopeptides have revealed to be an interesting class of supramolecular building blocks, being able to participate in different molecular recognition and self-assembling processes [1]. Thus, a new pseudopeptidic molecular cage derived from tripodal precursors with C 3 symmetry has been synthesized with the aim of studying its potential properties as receptor for biologically relevant anions [2]. These cage-like hosts are synthesised by the triple S N 2 reaction between tripodal tris(amido amines) and several 1,3,5-tris(bromomethyl)benzene electrophiles. Previous studies have shown that the cages derived from an aromatic electrophile with triple substitution in the ring (Me or Et) increase the chloride binding. N NH 2 O H N N HN H N O O NH 2 NH 2 Cl + F Cl F F Cl DIPEA Bu 4 NBr CH 3 CN HN O O N H HN F NH NH O F HN F Scheme 1. Macrocyclation for the synthesis of the fluorinated pseudopeptidic cages In this work, we have introduced specifically electronegative groups (fluorine) in the triple substitution of the ring [3], which is expected to modify its electronic density, and to provide the potential for an increased anion-π interaction inside the cage (see Scheme 1). References [1] E. Faggi, S. V. Luis, I. Alfonso. Current Medicinal Chemistry. DOI: / [2] I. Martí, M. Bolte, M. I. Burguete, C. Vicent, R. Quesada, I. Alfonso, S. V. Luis. Chem. Eur. J. 2012, 18, [3] S. M. F. Vilela, J. A. Fernandes, D. Ananias, L. D. Carlos, J. Rocha, J. P. C. Tomé, F. A. Almeida Paz. CrystEngComm. 2014, 16, 344. Acknowledgements: This work was partially supported by G.V. (PROMETEO ) and MINECO (CTQ R). E.Peris thanks MICINN for personal financial support (FPU13/00685). 65

66 P-21 ANCLAJE DE BALIZAS MOLECULARES A SOPORTES SÓLIDOS MEDIANTE QUÍMICA CLICK. APLICACIÓN A LA DETECCIÓN DE MICRORNAS ASOCIADOS A CÁNCER Y ESTUDIO DE PRESTACIONES Nazaret Peña 1, Daniel González-Lucas 1, María-José Bañuls 1,2, Ángel Maquieira 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain. (napegi@etsiamn.upv.es). 2 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain Palabras clave: Balizas moleculares, QCM, derivatización de superficies Los microrna son pequeños RNAs no codificantes, que regulan diversos procesos biológicos como proliferación, diferenciación y apoptosis celular [1]. Su desregulación puede desencadenar el desarrollo de ciertas enfermedades como el cáncer [2], que continúa siendo una de las principales causas de muerte en todo el mundo a pesar de los avances realizados en su detección y diagnóstico [3]. Es por ello que el desarrollo de nuevos dispositivos que permitan una detección temprana de la enfermedad se hace tan necesario. En el presente trabajo se desarrolla un estudio sobre la detección de mirna-155, asociado al cáncer de mama y de colon mediante el empleo de micromatrices con marcaje fluorescente y la tecnología sin marcaje de QCM. Para ello se emplea una baliza molecular, que sufre un cambio conformacional al hibridar con la hebra complementaria. Dichos cambios conformacionales se pueden emplear para mejorar las prestaciones de la hibridación ya sea mediante la unión de nanopartículas o estreptavidina a la baliza molecular. Además, es necesaria una inmovilización eficiente sobre el soporte, siendo llevada a cabo mediante la funcionalización de la superficie con trietoxivinilsilano, que interaccionará con las balizas moleculares gracias a los grupos tiol que presentan en uno de los extremos. El método de anclaje se basa en una reacción fotoinducida con luz ultravioleta próxima al visible, con alto rendimiento. Con el fin de mejorar la detección se anclan nanopartículas a las balizas moleculares analizando posteriormente con experimentos realizados en QCM los cambios conformacionales sufridos por la baliza molecular tras la hibridación con la sonda complementaria. El trabajo ha sido realizado bajo el marco del proyecto europeo del programa H2020 llamado SAPHELY (H2020-ICT ). Referencias: [1] Seven, M., Karatas, O. F., Duz, M. B, Ozen, M. Future Oncology, (6), [2] Reddy, K. B. Cancer Cell International (1). [3] Zhang, B., Pan, X., Cobb, G. P., Anderson, T. A. Developmental Biology, (1),

67 P-22 Inmunoensayo con detección luminiscente para la determinación de IgEs específicas de alergia a β-lactámicos. P. Quintero-Campos 1, S. Morais 1,2, A. Maquieira 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Camino de Vera s/n, E46022 Valencia, Spain. pedquica@upvnet.upv.es 2 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain. Palabras clave: IgE, determinante antigénico, antibiótico, ELISA luminiscente. Los antibióticos β-lactámicos son medicamentos habitualmente usados para combatir infecciones bacterianas. Las reacciones alérgicas relacionadas con la ingesta de antibióticos β-lactámicos son frecuentes y representan el 50% de las alergias debidas a fármacos. En la actualidad, los métodos de detección in vitro de alergias a fármacos permiten la detección de hasta 0,35 IU/mL de IgE específica en suero con una selectividad pobre, no permitiendo discriminar entre pacientes alérgicos y controles. Nuestro grupo de investigación ha puesto a punto un inmunoensayo heterogéneo para la determinación quimioluminiscente de IgE totales y específicas de alergia a antibióticos β- lactámicos. Basado en el típico ensayo ELISA, se utiliza un determinante antigénico de un antibiótico β-lactámico como antígeno inmovilizado. Las IgEs específicas presentes en el suero reconocen el determinante y se unen a él. Continuando con la metodología habitual de un ensayo ELISA se concluye el estudio midiendo la señal emitida por el sustrato añadido. En este trabajo se ha realizado un estudio de las prestaciones analíticas de inmunoensayos en placa ELISA. Los ensayos cromogénicos permiten la detección de IgE en el intervalo de concentración 0,35-0,70 U/mL, correspondiente a los pacientes alérgicos de clase 1, mientras que el ensayo con detección luminiscente presenta un límite de detección y cuantificación de 0,05 y 0,16 IU/mL, respectivamente, permitiendo la detección de pacientes alérgicos de clase 0, según la clasificación RAST. El ensayo desarrollado emplea un volumen de suero sanguíneo de 25 µl y se realiza en un tiempo total de 60 min. La especificidad de los determinantes antigénicos utilizados, así como la alta sensibilidad del ensayo, permiten cuantificar IgEs específicas de alergia a antibióticos de modo fiable y rápido. Agradecimientos: Este trabajo ha sido financiado por el programa H2020 (proyecto COBIOPHAD, grant agreement No ), y es una iniciativa de Photonics PPP ( 67

68 P-23 Self-propelled Janus platinum-mesoporous silica nanomotors for controlled drug delivery Paula Díez 1, Antoni Llopis-Lorente 1,2, Cristina de la Torre 1, Ramón Martínez-Máñez 1,2,3,4 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Spain. paudiesa@upvnet.upv.com 2 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). 3 Unidad Mixta de Investigación en Nanomedicina y Sensores. Universitat Politècnica de València, Instituto de Investigación Sanitaria La Fe, Valencia, Spain. 4 Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Valencia, Universitat Politècnica de València, Centro de Investigación Príncipe Felipe, Valencia, Spain. 3 keywords: Nanomotors, self-propulsion, platinum During the last years, the development of nanomachines with an efficient propulsion and cargo-towing attracted much attention due to their potential in biosensing, diagnostics and therapeutic applications [1]. In this context, synthetic nanomotors with propulsion mechanisms are promising vehicles in the design of autonomous nanomachines for an intelligent and controlled release of drugs [2, 3]. In this work, we report the design of a novel Janus-type nanomotor self-propelled by catalytic decomposition of hydrogen peroxide (H 2 O 2 ) for the intelligent administration of drugs. As illustrated in Figure 1, the new responsive nanomachines are constituted of the propelling nanoparticle element (platinum face), the drug-loaded nanocontainer (mesoporous silica face) and the disulfide-containing oligo(ethylene glycol) chains (SS- OEG) as a gating system. In the presence of both H 2 O 2 and glutathione, a faster release is observed due to the synergic effect of the self-propulsion (fueled by H 2 O 2 ) and the uncapping of the particles by glutathione. Such accelerated delivery is attributed to a local bubble generation in the platinum surface by the catalytic decomposition of H 2 O 2 into oxygen (gas) and water, resulting in a fluid mixing associated with such nanomotor movement. Figure 1. Self-propelled Janus platinum- mesoporous silica nanomotor: A) Schematic illustration, B) TEM image. References [1] F. Peng, Y. Men, Y.Tu, Yongming Chen, D.A. Wilson. Funct. Mater. 2018, [2] W. Gao, J. Wang. Nanoscale 2014, 6, [3] V.V. Singh, K. Kaufmann, B.E.F. de Ávila, M. Uygun, J. Wang. Chem. Comm. 2016, 52,

69 P-24 Fluorogenic detection of endotoxin using polymyxin B-capped mesoporous silica nanoparticles Ismael Otri, 1,2,3 Sameh El Sayed, 1,2,3 Elena Aznar, 1,2,3 Félix Sancenón 1,2,3 and Ramón Martínez-Máñez. 1,2,3 1 IDM, Instituto Interuniversitario de reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, camí de Vera s/n, Valencia. isot@doctor.upv.es 2 Departamento de Química. Universitat Politècnica de València. Camino de Vera s/n, Valencia. 3 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). keywords: (Endotoxin, Mesoporous Silica Nanoparticles, Polymyxin B) Endotoxin is a component of the outer cell membrane for all gram negative bacteria which is known biochemically as lipopolysaccharide (LPS) according to its component. Endotoxin immune response depends on LPS structure which contains lipid A and polysaccharide which give it toxicity and immunigicity features respectively. In addition, LPS is highly chemical stable molecule released by gram negative bacteria after death to the environment which leads to several health problems such as respiratory difficulties, pulmonary inflammation, asthma, fever, diarrhea and vomiting.[1,2] Therefore many studies about its detection are published but most of known methods are influenced by interferences in environmental samples like β-(1-3)-d-glucan and pectic polysaccharides from plant origin.[3] Polymyxin B (PMB) is a natural cyclic cationic peptide active against gram negative bacteria. According to its structure, which contains an hydrophilic and hydrophobic (lipophilic) part, has highly affinity to bind and neutralize lipid A in LPS.[4] Taking into account the above mentioned facts, a hybrid nanomaterial for LPS detection based on mesoporous silica nanoparticles (MSNs) loaded with rhodamine B and capped electrostatically using PMB was prepared. The designed MSNs keep blocked without rhodamine B release in the absence of LPS. In contrast it's uncapped selectively when LPS is present, which lead to the release rhodamine B. References [1] M.Mueller, B.Lindner, S.Kusumoto, K.Fukase, AB.Schromm, U.Seydel. J Biol Chem. 2004, 279: [2] J.Bhattacharyya, S.Biswas, A.G.Datta. Curr. Med. Chem. 2004, 11, [3] M.Peters, P.Fritz, A.Bufe. Innate Immun. 2012, 18(5): [4] D.Ferrari, C.Pizzirani, E.Adinolfi, S.Forchap, B.Sitta, L.Turchet, S.Falzoni, M.Minelli, R.Baricordi, F.D. Virgilio. J Immunol. 2004,173:

70 P-25 NOVEL N,N-DIPHENYLANILINO- HETEROCYCLIC ALDEHYDES BASED CHEMOSENSORS FOR UV-VIS/NIR Cu(II) DETECTION Hazem Essam Okda, 1 M. Manuela M. Raposo, 4 Félix Sancenón, 1,2,3 and Ramón Martínez-Máñez, * 1,2,3 1. Instituto Interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universitat Politècnica de València, Universitat de València, Spain. 2. Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022, València, Spain. haesel@etsid.upv.es 3. CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). 4. Centro de Química, Universidade do Minho, Campus de Gualtar, , Braga, Portugal. keywords: NIR detection, Cu(II) ion, N,N-diphenylanilino derivatives In recent years, the development of new colorimetric and fluorimetric molecular sensors for biologically active metal ions has been extensively investigated because of their potential applications in life sciences, medicine, chemistry, and biotechnology. [1] We report herein the synthesis and characterization of a novel chromo-fluorogenic probe (7) based on N,N-diphenylanilino heterocyclic aldehydes [2] for the selective detection of Cu(II) cation. Figure 1. UV-visible spectra of probe (7) (1.0 x 10-5 mol L -1 ) in acetonitrile alone and in the presence of 10 eq. of selected metal cations. UV/vis spectroscopic studies of probe (7) in pure acetonitrile in the presence of selected cations (Cu 2+, Pb 2+, Mg 2+, Ge 2+, Ca 2+, Zn 2+, Co 2+, Ni 2+, Ba 2+, Cd 2+, Hg 2+, Fe 3+, In 3+, As 3+, Al 3+, Cr 3+, Ga 3+, K +, Li +, Na + ) were carried out. Of all the cations tested, only Cu(II) is able to induce the appearance of intense absorption bands in the 625 and 1072 nm range with color modulation from faint yellow to deep violet (see Figure 1). References [1] P. R. Sahoo, K. Prakash, S. Kumar, Coord. Chem. Rev., 2018, 357, [2] a) D. P. Hagberg, T. Marinado, K. M. Karlsson, K. Nonomura, P. Qin, G. Boschloo, T. Brinck, A. Hagfeldt, L. Sun, J. Org. Chem., 2007, 72, ; b) C. Sissa, V. Parthasarathy, D. Drouin-Kucma, M. H. V. Werts, M. Blanchard-Desce, F. Terenziani, Phys. Chem. Chem. Phys., 2010, 12,

71 P-26 Desarrollo de microarrays de anticuerpos para la identificación de proteínas plasmáticas características de pacientes con enfermedad trombótica venosa Núria Fuster-Valls 1, Sergi Morais 1,2, Silvia Navarro 3, Ángel Maquieira 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Camí de Vera s/n, 46022, València, España. 2 Departamento Química, Universitat Politècnica de València, Camí de Vera s/n València, España. 3 Grupo de Investigación en Hemostasia, Trombosis, Arteriosclerosis y Biología Vascular. Instituto de Investigación Sanitaria La Fe. Avda. Fernando Abril Martorell, València, España. nufusval@etsiamn.upv.es LBP, microarray, trombosis Este trabajo se enmarca en un proyecto de investigación cuyo objetivo principal es validar un perfil de proteínas plasmáticas característico de pacientes con enfermedad trombótica venosa (ETV) y que previamente ha sido identificado mediante un abordaje con técnicas ómicas. Con el fin de validar el perfil clínico pronóstico de pacientes con patología trombótica se ha planteado, como prueba de concepto, el desarrollo de un método de diagnóstico que cumplan los requisitos de sensibilidad, selectividad, sencillez y portabilidad a un coste reducido para su implantación en los laboratorios clínicos de rutina. Como sistema modelo se ha estudiado la proteína de unión a lipopolisacáridos (LBP) que forma parte del perfil de proteínas característico de pacientes con ETV identificado previamente por análisis de proteómica diferencial no dirigido. La LBP es una glicoproteína de 58 kd que se une con alta afinidad a la porción de lípido A de lipopolisacáridos (LPS). El microarray para la determinación de LBP humana se basa en un inmunoensayo tipo sándwich, donde un anticuerpo monoclonal específico de captura de LBP se inmoviliza en la superficie de un disco DVD. El anticuerpo detector está marcado con biotina y la inmunointeracción se detecta con estreptavidina marcada con HRP y detección colorimétrica, usando tetrametilbencidina como sustrato y un lector de discos como detector. El ensayo se ha empleado para la identificación y cuantificación de LBP humana en plasma. El sistema desarrollado tiene potencial como método de screening para identificar individuos con alto riesgo de enfermedad trombótica. Agradecimientos: Este trabajo ha sido financiado por el programa de ayudas de proyectos de colaboración UPV-La Fe (2017/C28), y ayudas del ISCIII (PI14/00512). contrary, grafting method only allows to organic group to be grafted in external surface and near pore entrance, involving problems as oligomerization at the pore entrance locking the pores 3. 71

72 P-27 Effect of Pd annealing in the properties of Ni-Mo-P layer obtained by electroless process Nelly Ma. Rosas-Laverde 1-2, Jesús Cembrero 1, Javier Orozco-Messana 1, Alina Pruna 3 1 Department of Materials and Mechanical Engineering, Universitat Politecnica de Valencia, Valencia, Spain 2 Department of Materials, Escuela Politécnica Nacional, Quito, Ecuador 3 Center for Surface Science and Nanotechnology, Polytechnic University of Bucharest, Bucharest, Romania nelrola@doctor.upv.es 3 keywords: electroless, ceramic materials, Ni-Mo-P coating In order to extend the application of the ceramic materials, a metallic coating is often required. This can be achieved by different processes such as magnetic spraying, chemical and physical vapor deposition, electrodeposition and electroless deposition. The latter method is a low-cost process with ease in application and scaling. Moreover, it allows the obtainment of coatings with good homogeneity and uniform thickness, and improved properties such as service time or electrical conductivity [1-4]. The electroless process usually consists of two stages a) activation of the surface and b) metallization process by electroless. In this work, a Pd layer was deposited on the ceramic substrate by the airbrush technique. Next, the metallic layer of Ni-Mo-P was deposited by the electroless process on the activated ceramic samples. We considered the time and temperature of heat treatment to modified the final properties of the coating Ni-Mo-P and used this coating for backside contacts sensor devices. Structural characteristics, morphology, roughness and the electrical resistivity were measured by X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM) and four-point method using the Hall-effect method. The result showed that the Pd activation process have a significant influence on the final properties of the obtained films. References [1] X. Zheng, J. Tan, Q. Zhang, et al. Surf Coatings Technology, 2017, 311: [2] M. Alishahi, S. Monirvaghefi, A. Saatchi, S. Hosseini. Apply Surface Science. 2012, 258: [3] R. Guo, S. Jiang, Y. Zheng, J. Lan. J Appl Polym Sci. 2012, 127: [4] H. Wu, A. Susanto, K. Lian. Appl Surf Sci, 2017, 394: doi: /j.apsusc

73 P-28 SYNTHESIS AND CHARACTERIZATION OF A NEW L-VALINE PSEUDOPEPTIDE AS A CHIRAL LIGAND FOR STABLE METAL COMPLEXES Iván Muñoz, Belén Altava, Santiago V. Luis, Eduardo. García-Verdugo, M. Isabel Burguete Departamento de Química Inorgánica y Orgánica, Universidad Jaume I, Castellón, 12071, Spain. munozi@uji.es, luiss@uji.es keywords: Pesudopeptidic compounds, metal complexes, metal recognition. Metal chelation is involved in many biological processes and metal-based drugs have gained much importance for biological and medicinal applications [1]. Furthermore, organic ligands can be useful for selective extraction, removal or sensing of biologically and environmentally important species [2]. In this regard, one important strategy for ligand design is based on the inclusion of amino acid residues in order to prepare stable complexes allowing to understand the interaction of proteic structures with metals [3]. The building of small model compounds is a common approach used for the understanding of the structural parameters and factors determining the properties of interest in proteins and natural systems. [4] O OH NHCB z z H 2 N + N ii)) iii)) O NH N NHCB z z iiiii) ii v v) N O N HN OH N Scheme 1. Synthesis. (i) DCC, N-hidroxysuccinimide, THF, 18 h, 0ºC, 80 85%; (ii) 2-picolylamine, THF, 20h, r.t., 75-80%; (iii) H 2 /Pd/C, 12 h, MeOH, r.t., 60 70%; (iv) 4-(Diethylamino)salicylaldehyde, 12 h, MeOH, r.t., 60 70%; [1]. Regarding metal complexation, previously our group have studied the behavior and coordination of C2 symmetrical bis(amino amides) derived from amino acids towards Cu(II) and Zn(II) ions [5]. In this work, we report the design, preparation and synthesis of a new pseudopeptidic ligand derived from L-valine (figure 1), as well as the characterization and analysis of their binding ability towards different M(II) species. References [1] R.R. Crichton, D.T. Dexter, R.J. Ward, Coord. Chem. Rev. 2008, 252, [2] L. Gorla, V. Martí-Centelles, B. Altava, M.I. Burguete, S.V. Luis, Dalton Trans , [3] J. Paradowska, M. Pasternak, B. Gut, B. Gryzlo, J. Mlynarski; J. Org. Chem. 2012, 77, [4] S. V. Luis and I. Alfonso, Acc. Chem. Res. 2014, 47, [5] (a) S. Blasco, M. I. Burguete, M. P. Clares, E. García-España, J. Escorihuela, S. V. Luis. Inorg. Chem. 2010, 490, (b) I. Martí, A. Ferrer, J. Escorihuela, M. I. Burguete and S. V. Luis. Dalton Trans. 2012, 41, Acknowledgements. Financial support from MINECO (CTQ R) and Generalitat Valenciana (Prometeo ). 73

74 P-29 PREPARATION AND CHARACTERIZATION OF IONIC AMIDE-BASED [2]ROTAXANES Fátima Morales, Verónica Susana Velásquez, Mateo Alajarín, José Berná*. Departamento de Química Orgánica, Facultad de Química, Campus de Excelencia Internacional Regional "Campus Mare Nostrum", Universidad de Murcia, Murcia. 3 keywords: supramolecular chemistry, rotaxanes, template synthesis The supramolecular entities based on non-covalent ensembles enable multiple applications, such as stimuli-responsive polymeric materials [1]. One of the strongest noncovalent interactions are ionic bonds. These interactions have been employed to produce supramolecular networks with changing viscosity [2]. [2]Rotaxanes are the most abundant category among the different types of mechanicallyinterlocked molecules (MIMs). They have been a hot topic in many scientific areas, including materials [3] and nanotechnology [4] in the last years. These molecules that are composed by a dumbbell-shaped component threaded through a ring with non-covalent bonding interactions hold a unique kind of functionality due to the mechanical bond [5]. One of the template-directed synthesis of these MIMs is based on hydrogen-bonding interaction [6, 7]. Herein, we present novel tetraamide-based [2]rotaxanes, their synthesis and characterization, aimed to prepare candidates for supramolecular ionic networks. Acknowledgments This work was supported by the MINECO (CTQ P) with joint financing by FEDER Funds from the European Union, and Fundación Seneca-CARM (Project 19240/PI/14). F.M. also thanks the Fundación Seneca-CARM for her Saavedra Fajardo contract and funding (Contract No /SF/16). References [1] B. Rybtchinski. ACS Nano, 2011, 5(9), [2] a) M.A. Aboudzadeh, M.E. Muñoz, A. Santamaría, M.J. Fernández-Berridi, L. Irusta, D. Mecerreyes. Macromolecules, 2012, 45, b) M. Wathier, M.W. Gringstaff. J. Am. Chem. Soc., 2008, 130, c) S. Cheng, F.L. Beyer, B.D. Mather, R.B. Moore, T.E. Long. Macromolecules, 2011, 44, [3] Y. Wang, M. Frasconi, J.F. stoddart. ACS Cent. Sci., 2017, 3, [4] C. Alvarez-Lorenzo, C.A. García-González, A. Concheiro. J. Control. Release, 2017, 268, [5] G. Barin, R.S. Forgan, J.F. Stoddart. Proc. R. Soc. A., 2012, 468, [6] A. Martínez-Cuezva, A. Saura-Sanmartín, T. Nicolás-García, C. Navarro, R.A. Orenes, M. Alajarín, J. Berná. Chem. Sci., 2017, 8, [7] F. G. Gatti, D. A. Leigh, S. A. Nepogodiev, A. M. Z. Slawin, S. J. Teat, J. K. Y. Wong. J. Am. Chem. Soc., 2001, 123,

75 P-30 SURFACE DERIVATIZATION, WETTABILITY, HAPTEN-PROTEIN AND PROTEIN-PROTEIN BINDING PROPERTIES Miguel Ángel González-Martínez, Pilar Aragón, Patricia Noguera, María José Bañuls, Rosa Puchades, Ángel Maquieira IDM, Universitat Politècnica de València, camino de vera s/n, Surface derivatization, wettability, antibody-antigen binding The influence of surface wettability on array biosensing properties has been studied, for ligand-protein and protein-protein interactions, in a typical indirect immunoassay arrangement. On the basis on previous achievements using biotin-streptavidin interaction as model, glass substrate chips were modified with different alkenyl and fluoroalkenyl silanes, and their mixtures, in order to set surface wettability, and a thiol-biotin derivative hapten was anchored by means of the photochemical click thiol-ene reaction. Finally rabbit anti-biotin polyclonal antibody was allowed to react with derivatized surfaces, followed by a secondary Alexa-Fluor 647 labelled goat anti-rabbit IgG (see figure). Results show that lowest unspecific binding and highest signal intensity and SNR, are obtained with mild hydrophobicity achieved with large silanes (C 21 and C 22 ), while the shortest silane (C 02 ) gives rise to lowest hydrophobicity and poor data. Best results are achieved when modifying alkenyl silanes with 1% fluorinated alkyl silane, which is traduced in the same hydrophobicity (water contact angle in the range º) for all surfaces, complete removal of labelled secondary antibody unspecific binding, and improved SNR, especially for the C 02 silane, increasing its value till ca. 3000, allowing achievement of good-performing surfaces employing more convenient reagents. O NH H HN S H O NH H HN S H H N O H N O C 22 O O O O O O C 11 C 10F C 10F* C 02 H N O S C 03 H N O S C 08 F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F Si O O Si O O O O OH OH Si O O Si O O O O OH Si O O O Si O O O Si O O O Si O O OH OH O Si O O O Si O O OH OH O Si O O O OH Si O O OH OH O Si O O O Si O O OH OH OH O Si O O O Financial support from the GVA (PROMETEO/2014/40), FEDER and the Spanish MINECO (CTQ R) is acknowledged. 75

76 P-31 BACTERIAL RECOGNITION: RELEVANT TIME OUTPUTS DERIVED FROM A SINGLE SCREENING BIODEGRADABILITY ASSAY DATA María José Medina-Hernández 1, Laura Escuder-Gilabert 1, Yolanda Martín-Biosca 1, Mireia Pérez-Baeza 1, Salvador Sagrado 1,2 1 Departamento de Química Analítica, Universitat de València, Burjassot, Valencia, Spain. Maria.j.medina@uv.es 2 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM). Universitat Politècnica de València, Universitat de València. Avd. Vicente A. Estellés s/n, Burjassot, Valencia, Spain. Keywords: bacterial recognition, accuracy, modelling Active pharmaceutical ingredients and their metabolites have been frequently detected in the environment are often resistant due to inefficient degradation at wastewater treatment plants [1]. The OECD tests for ready biodegradability have been devised as screening methods to determine whether a chemical is potentially easily biodegradable. In this communication, OECD-like biodegradability assay results are simulated, using a minimum experimental effort strategy; i.e. one single time, t, to obtain the corresponding biodegradation (BD %) data. With the corresponding BD-t data pair, and assuming common biodegradation models (Fig. 1A), several relevant time-variables are estimated (i.e. half-live time, t50, and times were the curve reaches BD values of 90 and 99.9 %, t90 and t99.9, respectively). An approach to correct the estimates (Ct50, Ct90, Ct99.9) is tested in order to improve results (Fig. 1B). The influence of several factors affecting the accuracy of the estimations is evaluated. The procedures are finally applied to experimental data. Fig.1. (A) Single BD-t data (o), Monod model (solid line). (B) Boxplot of 100 simulations with RSD = 5 % in BD-t data. Acknowledgements This work has been supported by the Project CTQ R (MINECO/FEDER, UE). References [1] K. Kummerer. Pharmaceuticals in the Environment. Springer-Verlag, Berlin,

77 P-32 Determinación simultánea de IgE e IgG específica de alérgeno mediante multiplexado Salvador Mas 1, Natalia Casañ-Raga 1, Ahmed Ali 1, Dolores Hernández Fernández de Rojas 2, Ethel Ibáñez-Echevarría 2 Ángel Maquieira 1,3, Sergi Morais 1,3 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Spain 2 Hospital Universitari i Politènic La Fe, Servicio de Alergología, Avinguda de Fernando Abril Martorell, 106, València, Spain 3 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia, Spain salmaga1@upvnet.upv.es 3 palabras clave: Alergia, inmunoglobulina E y G, perfil de sensibilización. Las enfermedades alérgicas, debidas a reacciones de hipersensibilidad de tipo I constituyen la enfermedad crónica con mayor prevalencia en países en desarrollo. Su diagnóstico genera elevados costes al sistema sanitario, haciendo necesario el uso pruebas diagnósticas entre las cuales se encuentra la cuantificación mediante ensayos in vitro de la molécula mediadora en esta enfermedad, la inmunoglobulina E (IgE) específica para los diferentes alérgenos [1]. Sin embargo, la presencia de inmunoglobulina G (IgG) se ha relacionado con la exposición alergénica así como con los fenómenos de inducción de tolerancia, espontánea o bien tras procesos de desensibilización mediante inmunoterapia. La presencia de ambas inmunoglobulinas específicas para un panel representativo de alérgenos puede detectarse de forma simultánea. En este trabajo, se presenta el desarrollo de un inmunoensayo directo basado en microarrays para la determinación de inmunoglobulinas totales y específicas E y G mediante tecnología de discos compacto. Como prueba de concepto, doce alérgenos se han inmovilizado en un disco DVD y las IgE e IgG específicas se detectaron, utilizando anticuerpos monoclonales anti-ige y anti-igg marcados con oro. El ensayo presenta rango dinámico de 0,4 a 50 IU/mL de IgE. El microarray en disco se utilizó para el análisis de 119 muestras de suero humano con diferentes perfiles de sensibilización. Los resultados se compararon con los obtenidos por el método in vitro de referencia. De acuerdo al análisis ROC, el área bajo la curva obtenida para 10 de 12 alérgenos varió entre 0,93.y 1.0, lo que indica la buena sensibilidad y especificidad de ensayo. El valor de correlación encontrado entre ambos métodos para los niveles de IgE total fue de 0,86, mientras que para IgE específica de alérgeno varió de 0,90 a 0,98. [1] Golden Standards for Allergy Diagnosis. EAACI White Book 2018, p Agradecimientos: Este trabajo ha sido financiado por el programa FEDER, GVA PROMETEO II/2014/040 y MINECO CTQ R. 77

78 P-33 ON-CHIP ALLELE SPECIFIC HYBRIDIZATION ONTO MAGNETIC PARTICLES Sara Martorell 1, Ángel Maquieira 1,2,3 Luis A. Tortajada-Genaro 1,2,3 1 Unidad Mixta UPV-La Fe, Nanomedicine and Sensors, IIS La Fe, Valencia, Spain 2 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM). Universitat Politècnica de València-Universitat de València. Camino de vera s/n, Valencia. 3Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain samarte@upvnet.upv.es Magnetic particles, hybridization, microfluidics. Nucleic acids are excellent biomarkers of human diseases. Although solid-phase hybridization is an interesting working format for the specific detection of genomic variants, conventional methods need long incubation times and numerous washing steps. In this communication, we report a rapid method for homogenous DNA hybridization. Magnetic particles were used as a solid support for anchoring allele specific probes [1]. For assay automation, disposable polymer microfluidic chips with small-volume reaction (20 μl) chambers were studied. Under the selected experimental condition, the particles were efficiently captured by a magnet in the detection zone (yield up to 95 %). The signal fluorescence emitted by the Cy5 and FAM labelled perfect-match complexes was registered by fluorescence microscopy. The analytical performances of the developed system were excellent compared to previous platforms [2]. As proof of concept, the method was applied for the detection of mutations located in KRAS oncogene because these genetic variants are relevant diagnostic markers in cancer (colorectal, lung among others) [3]. Genomic DNA was extracted using robotic workstation from Formaldehyde fixed-paraffin embedded tissues and PCR method amplified the target region. The magnetophoretic-based approach for target-specific DNA hybridization identified correctly the presence of mutations. Therefore, the results show a high selective and sensitive method to discriminate wild-type and mutant variants even in complex human tissues. ACKNOWLEDGMENTS: Financial support from FEDER, GVA PROMETEO II /2014/040, MINECO CTQ R and S.Martorell contract grant (Technical Support Personnel PTA- 2016). References [1] Ravan, H. Analytical Biochemistry , [2] Hernández-Neuta, I. Biosensors & Bioelectronics , [3] Misale, S. Nature ,

79 P-34 A new environmentally friendly probe for formaldehyde gas detection in real conditions Carlos Martínez 1, Ana M. Costero 1,2,3, Pablo Gaviña 1,2,3, Salvador Gil 1,2,3 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM). Universitad Politècnica de València, Universitat de València, Doctor Moliner 50, Burjassot, 46100, Valencia, Spain. 2 Departamento de Química Orgánica, Universitat València, Doctor Moliner 50, Burjassot, 46100, Valencia, Spain. 3 CIBER de Bioingeniería, Biometariales y Nanomedicina (CIBER-BBN) (Spain). carlos.martinez-aquino@uv.es Formaldehyde, environmentally friendly probe, RGB analysis Indoor formaldehyde contaminant is a general health problem due to his volatility and widespread use. The highest levels of formaldehyde are found in work settings where formaldehyde is used or produced. The Pictet-Spengler [1] reaction of primary catecholamines with formaldehyde followed by aerobic oxidation of the generated tetrahydroisoquinoline has been used for the histochemical demonstration of biogenic monoamines by using fluorescence microscopy [2]. We now report the application of this process, which uses environmental friendly compounds, for detecting formaldehyde. Our probe, consisting of and aqueous solution of dopamine containing glycine and sucrose, allows the colorimetric detection of formaldehyde in solution. In order to extend our study, we also prepared test strips for the colorimetric detection of formaldehyde gas. To quantify this effect, RGB values were measured from the photographs of the exposed probes using image-processing software (ImageJ) and compared with blank probes. References [1] E. D. Cox, J. M. Cook, Chem. Rev. 1995, 95, [2] G. Jonsson, Acta Chem. Scand., 1966, 20,

80 P-35 BIOMARKERS FOR BLADDER CANCER IN URINE BY NMR SPECTROSCOPY Alba Loras Monfort 1, M.Carmen Martinez-Bisbal 1,2, Guillermo Quintás Soriano 3,4, Salvador Gil Grau 2,5, Ramón Martínez-Mañez 1,2,6,7, José L. Ruiz-Cerdá 1,8. 1 Unidad Mixta de Investigación en Nanomedicina y Sensores. Instituto de Investigación Sanitaria La Fe, Universitat Politècnica de València. Valencia, Spain mamarbis@upvnet.upv.es 2 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, Universitat de València, Valencia, Spain 3 Unidad Analítica, Instituto de Investigación Sanitaria La Fe, Valencia, Spain 4 Leitat Technological Center, Bio in vitro Division, Valencia, Spain 5 Departamento de Química Orgánica, Facultad de Químicas, Universitat de València, Valencia, Spain 6 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). Spain 7 Departamento de Química, Universitat Politècnica de València, Valencia, Spain 8 Servicio de Urología, Hospital Universitario y Politécnico La Fe, Valencia, Spain Bladder Cancer, metabolomics, NMR spectroscopy Metabolomics is a powerful tool in the oncologic research.. The more commonly used techniques are NMR spectroscopy and mass spectrometry (MS). NMR provides metabolical information with a high specificity in molecules in solution, with milimolar concentration. This is a highly reproducible technique, non-destructive and allows the study of the sample almost intact. This study aims for the identification of a metabolomical fingerprint in urine related to Bladder Cancer (BCa) by the study of metabolites in urine by 1 H NMR spectroscopy. A total of 102 urine samples from 69 patients with BCa were collected before and after- TUR. The urine samples after-tur were assumed as control, free of cancer. The global metabolical profile was obtained by 1 H NMR spectroscopy. The spectra were processed and normalized to the signal of Creatinine. The whole set of samples was split in two groups, 65 samples for calibration (43 before-tur and 22 after-tur), and 37 samples for validation (26 before-tur and 11 after-tur). A supervised discriminant analysis was performed by Partial Least Squares-Discriminant Analysis (PLS-DA). The discriminant and generalization ability of the model was evaluated according to the sensibility, specificity, negative predictive value (NPV) and positive predictive value (PPV). The PLS-DA model showed differences in the metabolic profile in the urine samples from BCa before and after-tur For the validation set in the PLS-DA analysis, a sensibility of 92.3%, specificity of 72.7%, PPV of 88.9%, and NPV of 80.0% were obtained, with an area under ROC curve of The study of urine in patients with BCa by NMR spectroscopy showed findings that may predict the presence of BCa when compared with urine from patients after TUR (free of cancer). This strategy stand for an option for the non-invasive diagnosis and follow-up in BCa with appropriated sensibility and specificity. 80

81 P-36 QUANTITATIVE DETERMINATION OF BIOMARKERS IN URINE FOR PROSTATE CANCER BY NMR SPECTROSCOPY M.Carmen Martinez-Bisbal 1,2, Natividad Sebastiá 1, Claire Cannet 3, Hartmut Schaefer 3, Alba Loras Monfort 1, Martial Piotto 4, Ramón Martínez-Mañez 1,2,5,6, Leo Marzullo-Zucchet 7 José L. Ruiz-Cerdá 1,7. 1 Unidad Mixta de Investigación en Nanomedicina y Sensores. Instituto de Investigación Sanitaria La Fe, Universitat Politècnica de València. Valencia, Spain mamarbis@upvnet.upv.es 2 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, Universitat de València, Valencia, Spain 3 Bruker BioSpin GmbH, Rheinstetten, Germany 4 Bruker BioSpin, Wissembourg, France 5 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). Spain 6 Departamento de Química, Universitat Politècnica de València, Valencia, Spain 7 Servicio de Urología, Hospital Universitario y Politécnico La Fe, Valencia, Spain Prostate Cancer, metabolomics, NMR spectroscopy Metabolomics is a powerful tool in the oncologic research. Metabolomics studies the metabolites that are in biological samples (metabolome) and identifies the fingerprint of the tumoral process. The more commonly used techniques are NMR spectroscopy and mass spectrometry (MS). NMR provides metabolical information with a high specificity in molecules in solution, with milimolar concentration. This is a highly reproducible technique, non-destructive and allows the study of the sample almost intact. This study aims for the identification of a metabolomical fingerprint in urine related to Prostate Cancer (PCa) by the determination of metabolite concentration in urine by NMR spectroscopy. A total of 134 urine samples from 57 patients with PCa and 77 patients with BPH were collected. NMR was used to perform a quantitative determination of 131 metabolites. The whole set of samples was split in two groups, 93 samples for calibration (43 PCa and 52 BPH), and 41 samples for validation (14 PCa and 27 BPH). The metabolite concentrations were normalized with the sum of concentration of all metabolites. A supervised discriminant analysis was performed by Partial Least Squares-Discriminant Analysis (PLS-DA). The discriminant and generalization ability of the model was evaluated according to the sensibility, specificity, negative predictive value (NPV) and positive predictive value (PPV). The model robustness was evaluated with Permutation tests. The PLS-DA model showed a metabolic profile different in the urine samples from PCa and BPH. For the validation set a sensibility of 93%, specificity of 75% were obtained, with NPV of 95% and PPV of 65%. The quantitative metabolical determination in urine of patients with PCa and BPH by NMR reveals differences between both groups that could allow the presence of PCa. This strategy stand for an option for the non-invasive diagnosis of PCa patients, with appropriated sensibility and specificity. 81

82 P-37 ANALYSIS OF THE REVERSIBILITY OF CHARGE-TRANSFER IN FARADIC PROCESSES A. Martinez-Ibernón 1, J.R. Lliso-Ferrando 1, I. Gasch 1, J.M. Gandia-Romero 1, J. Soto 1 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain. anmarib@arqt.upv.es 3 keywords: Faradic processes, equivalent circuits, charge curve With the purpose of modeling the real corrosion systems, electrochemistry techniques are applied, being the most widely used the voltametric technique. In these tests, a variable potential signal is applied in the working electrode, taking as a reference potential the open circuit potential (OCP). In this way when the potentials applied are lower than the OCP, cathodic reactions (reduction) are produced in the working electrode (WE) conversely; when the applied potential is higher than OCP, anodic reactions (oxidation) are occurred in the WE. As a result of the voltametric test, a response current signal is recorded. This is interpreted through an equivalent electric circuit, being the most simple the Rs-(Rp/Cdl) circuit [1]. Figure 1 Scheme of Rs-(Rp/Cdl) equivalent circuit. Self-source. The main objective of the present study is to prove that the charge transferred during the anodic processes and cathodic reactions are different. Therefore, it is necessary to define an Rp for each half-reaction. In order to do this, three different REDOX systems from the point of view of the chargetransfer reversibility were studied, using the voltametric technique developed by Alcañiz et al. [2]. Therefore, different dissolutions with Hexacyanoferrate (II) and Hexacyanoferrate (III) were prepared, and Au electrodes were used. The tests were done in Argon atmosphere. Through analysis and comparison of the accumulated charge curves obtained by means of the current results, we could justified the necessity to define two Rp in the equivalent simple circuit defined in the figure 1. References [1] Guojian, L., Yunsheng, Z., Meng, W., & Ran, H. (2017). Study of depassivation of carbon steel in simulated concrete pore. Construction and Building Materials, [2] Alcañiz M., Bataller R., Gandía-Romero J.M, Ramón J.E., Soto J., Valcuende M., Universitat Politècnica de València, Sensor, red de sensores, método y programa informático para determinar la corrosión en una estructura de hormigón armado, nºes (Patente de Invención concedida con Examen Previo) mayo 06,

83 P-38 ENANTIORECOGNITION OF ORGANIC CONTAMINANTS: MODELLING THE ENANTIORESOLUTION OF STATIONARY PHASES IN RPLC María José Medina-Hernández 1, Laura Escuder-Gilabert 1, Yolanda Martín-Biosca 1, Mireia Pérez-Baeza 1, Salvador Sagrado 1,2 1 Departamento de Química Analítica, Universitat de València, Burjassot, Valencia, Spain. Yolanda.martin@uv.es 2 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM). Universitat Politècnica de València, Universitat de València. Avd. Vicente A. Estellés s/n, Burjassot, Valencia, Spain. Keywords: Stationary phase enantiorecognition, organic contaminants, modelling Chiral molecules play an important role in all aspects of life sciences [1]. Due to its simplicity and accuracy, high-performance liquid chromatography (HPLC) with chiral stationary phases (CSPs) is the most used analytical technique for separation of enantiomers [1]. In this communication, structural information of neutral and basic compounds is used to model their enantioresolution levels obtained from an immobilized cellulose tris(3,5-dichlorophenylcarbamate) stationary phase in reversed phase conditions. Three topological parameters (two of them connected to the chiral carbon) and six molecular descriptors (one of them also related with the chiral carbon) are selected after a discriminant partial least squares refinement process (DPLS1). Categorical enantioresolution levels (RsC, 0 = no baseline enantioresolution, 1 = baseline enantioresolution, 0.5 = almost enantioresolution) can be discriminated using the refined model by means of the score plot of the first two latent variables (LV) (Fig. 1). Scores LV Scores LV1 Fig.1. Discriminant ability of the DPLS1 model for the three RsC levels (32 organic compounds). Acknowledgements This work has been supported by the Project CTQ R (MINECO/FEDER, UE). References [1] M. Lämmerhofer. J. Chromatogr. A. 2010, 1217,

84 P-39 El bloqueo del receptor A 2B incrementa el daño inducido por TPA en un modelo de hiperplasia en piel de ratón Marín A., a,b Payá, M. a,b, Montesinos, M.C. a,b, Terencio, M.C. a,b a. Departamento de Farmacología, Facultad de Farmacia. Universidad de Valencia. Av. Vicent Andrés Estelles s/n Burjassot (Valencia) b. IDM, Instituto Interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico. Universidad de Valencia. C/Dr. Moliner Burjassot (Valencia) macasm@alumni.uv.es 3 palabras clave: Receptor A 2B, BAY , PSB-1115 La adenosina es un regulador endógeno de la inflamación que ejerce su acción mediante la activación de sus receptores de superficie A 1, A 2A, A 2B y A 3 [1]. Recientemente se ha puesto de manifiesto que la activación del receptor de adenosina A 2B, mayoritario en queratinocitos, presenta efecto antiproliferativo y antiinflamatorio [2]. En el presente estudio determinamos el efecto in vivo de un agonista, BAY (BAY), y un antagonista, PSB-1115 (PSB), del receptor A 2B en el modelo de hiperplasia en piel de ratón inducida por 12-O-tetradecanoilforbol-13-acetato (TPA). BAY (10 µg/sitio), PSB (50 µg/sitio) o ambos, fueron aplicados tópicamente en el dorso depilado de ratones hembra Swiss, 30 minutos antes de la aplicación de TPA (2 nmol/sitio) durante 3 días consecutivos. La evolución de las lesiones en la piel fue evaluada visualmente mediante una escala de 0-4. Al cuarto día los animales fueron sacrificados y se obtuvieron biopsias de 1 cm 2 que fueron pesadas y procesadas para su posterior análisis histológico. Las lesiones producidas tras aplicar TPA (grupo control) fueron atenuadas en los ratones tratados con BAY mientras que la aplicación de PSB incrementó el daño tisular. Paralelamente, BAY redujo el edema desarrollado por TPA (110,2±5,7mg vs.138,7±6,2mg) mientras que, tanto PSB como la combinación de BAY+PSB presentaron unos valores de edema similares al grupo control. Estos datos se confirman en el estudio histológico donde la tinción eosina-hematoxilina y la detección de ki67 (marcador de proliferación celular) revelaron un aumento de la hiperplasia epidérmica y de la proliferación de queratinocitos en ratones tratados tanto con TPA como con PSB, mientras que la aplicación de BAY redujo ambos parámetros. Todos estos resultados sugieren que la activación del receptor A 2B puede representar una diana farmacológica para el tratamiento de patologías que presentan una respuesta inflamatoria e hiperproliferación epidérmica, como la psoriasis. Referencias: [1] BN. Cronstein, M. Sitkovsky. Nat Rev Rheumatol., 2017, 12., [2] RM. Andrés, MC. Terencio, J. Arasa. JID., 2017, 137.,

85 P-40 AMINO-FUNCTIONALIZED CELLULOSE PARTICLES FOR REMOVAL OF LEGIONELLA PNEUMOPHILA FROM WATER María Ruiz-Rico 1, Yolanda Moreno 2, José M. Barat 1 1 Grupo de Investigación e Innovación Alimentaria. Departamento de Tecnología de Alimentos, Universitat Politècnica de València. Camino de Vera s/n, 46022, Valencia, Spain maruiri@upvnet.upv.es 2 Instituto de Ingenieria del Agua y Medio Ambiente (IIAMA), Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain Surface functionalization, cellulose microparticles, filtration Legionella pneumophila is a pathogenic microorganism responsible for legionellosis. Legionella causes this serious lung disease through the inhalation of aerosols from contaminated water. Different disinfection methods involving physical (e.g., heat, UV irradiation, filtration), and chemical (e.g., chlorine, metal ions) treatments has been proposed to ensure water safety [1]. Filtration technologies have stood out in recent years, but they are limited by membrane fouling [2]. The immobilization of bioactive compounds onto surfaces can prevent fouling of the filtration surfaces. In this work, commercial cellulose microparticles were functionalized with polyamines to design antimicrobial material for the filtration of water through a bed of the developed particles. Thus, the objective of this work was (i) to assess the antibacterial activity of equivalent concentrations of the free and immobilized polyamines against L. pneumophila, and (ii) to evaluate the applicability of the amine-functionalized particles as filtering elements to remove microbial contamination. The in vitro antimicrobial activity of the aminefunctionalized cellulose particles against L. pneumophila, compared to the same dose of free amines, was assessed by culture and fluorescent viability staining (L/D BacLight Bacterial Viability Kit). The plate count results showed total loss of culturability after incubation with free and immobilized polyamines. In addition, fluorescence microscopy confirmed the inhibition of L. pneumophila because of damage of cell membrane, which prevents the persistence of the bacterium in the viable but non-culturable state [3]. This effect may be due to attractive binding forces between the positive amine corona on the surface of particles and the negatively charged bacteria membrane that induce a disruption of the cell membrane. Finally, water contaminated with L. pneumophila was filtered through a bed of antimicrobial particles. The amine-functionalized cellulose microparticles used as filtering elements displayed significant inhibitory activity of the pathogenic microorganism, demonstrating the high potential of the developed particles in water treatment. References [1] Dupuy, M. Mazoua, S. Berne, F. Bodet, C. Garrec, N. Herbelin, P. Ménard-Szczebara, F. Oberti, S. Rodier, M.H. Soreau, S. Wallet, F. Héchard, Y. Water Research. 2011, 45(3), [2] Li, Q. Mahendra, S. Lyon, D. Brunet, L. Liga, M. Li, D. Alvarez, P. Water Research. 2008, 42(18), [3] Alleron, L. Merlet, N. Lacombe, C. Frère, J. Current Microbiology. 2008, 57(5),

86 P-41 ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL COMPONENTS AGAINST FOOD MICROORGANISMS A COMPARATIVE STUDY BETWEEN FREE AND ENCAPSULATED COMPOUNDS IN VAPOUR PHASE Adina-Alexandra Baicu 1, María Ruiz-Rico 2, Mona-Elena Popa 1, José M. Barat 2 1 University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Mărăști Blvd., District 1, Bucharest, Romania 2 Grupo de Investigación e Innovación Alimentaria. Departamento de Tecnología de Alimentos, Universitat Politècnica de València. Camino de Vera s/n, 46022, Valencia, Spain maruiri@upvnet.upv.es Essential oil components, encapsulation, mesoporous silica microparticles The inhibition of microbial growth through the addition of natural antimicrobial preservatives have attracted considerable attention due to the increased consumer awareness on the aspects of food quality and safety [1]. The application of essential oils components (EOCs) as food preservative is limited by their high volatility and reactivity. Encapsulation of bioactive compounds has become an efficient approach to increasing their physical stability protecting them against environmental factors, reducing their volatility and preventing the interactions with the food ingredients [2]. The antimicrobial activity of EOCs has been extensively studied using a direct-contact antimicrobial assays, but the use of these bioactive compounds in vapour phase have shown increased antimicrobial efficiency [3]. The present study aims to provide a comparative analysis between the inhibitory effect in vapour phase of free and encapsulated carvacrol, cinnamaldehyde, eugenol and thymol against three different classes of microorganisms (Listeria innocua, Aspergillus niger and Zygosaccharomyces rouxii). The EOCs were encapsulated in mesoporous silica particles to reduce the rate of evaporation of the compounds via vapour adsorption [4]. After characterization of the loaded microparticles and determination of loading capacity, the antimicrobial activity of vapours of microencapsulated and non-microencapsulated EOCs were evaluated by the vapour diffusion test [5]. The results show that both free and encapsulated compounds prove to be effective in inhibiting or retarding the growth of the selected microorganisms. This indicated that the efficiency of EOCs-loaded in mesoporous silica microparticles did not affect by the encapsulation process. The encapsulated compounds tend to generally be efficient for a longer period than free EOCs. The encapsulation of bioactive compounds showed enhancement of the antimicrobial activity after several days of incubation, which is correlated with the speed of evaporation. The controlled release of the EOCs after their encapsulation in mesoporous silica microparticles demonstrate their potential application in the food industry. References [1] Weiss, J. Gaysinksy, S. Davidson, M. McClements J. Global Issues in Food Science and Technology, Elsevier Inc., Amsterdam, [2] Donsì, F. Annunziata, M. Sessa, M. Ferrari, G. LWT-Food Science and Technology. 2011, 44(9), [3] Božik, M. Císarová, M. Tančinová, D. Kouřimská, L. Hleba, L. Klouček, P. Industrial Crops and Products. 2017, 98, [4] Bernardos, A. Marina, T. Žáček, P. Pérez Esteve, É. Martínez Mañez, R. Lhotka, M. Kouřimská, L. Pulkrábek, J. Klouček, P. Journal of the Science of Food and Agriculture. 2015, 95(14), [5] Goni, P. López, P. Sánchez, C. Gómez-Lus, R. Becerril, R. Nerín, C. Food Chemistry. 2009, 116(4),

87 P-42 Biorthogonal strain-promoted azide-alkyne cycloaddition for the fluorescent detection of senescent cells through lipofuscin labelling. Beatriz Lozano-Torres 1,2,3, Juan F. Blandez 1,2, Irene Galiana 1,2,3, Félix Sanzenón 1,2,3,4 and Ramón Martínez-Máñez 1,2,3,4 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Camí de Vera s/n, Valencia. Spain. bealotor@upvnet.upv.es 2 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). 3 Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Universitat Politècnica de València, Centro de Investigación Príncipe Felipe, Valencia, Spain. 4 Unidad Mixta de Investigación en Nanomedicina y Sensores. Universitat Politècnica de València, IIS La Fe, Valencia, Spain Keywords: Senescent cells, lipofuscin, biorthogonal reactions. The early detection of senescent cells could be an innovative strategy to identify different diseases, such as cancer or idiopathic pulmonary fibrosis [1]. On the other hand, molecular bioimaging for in vivo enzyme activity using non-invasive smart fluorescent molecular probes has become a matter of concern in the last 5 years [2]. M. Serrano y VG. Gorgoulis reported that the specific lipofuscin staining with Sudan Black B (SBB) is a powerful tool for senescence detection in fixed tissue [3]. Positive samples for lipofuscin staining were also positive for Senescence Associated beta galactosidase activity (SA β gal) a kit normally used to distinguish senescent cell from control cells. Taking these facts into account we present herein a new method to differentiate senescent cells from control cells through a biorthogonal reaction. Biorthogonal are a powerful approach for the study of biological processes in their native environment [4]. The main characteristics of these reactions are high selectivity and efficiency, and functionality in the complex biological environment of cells [5]. The prototype of this group of reactions is the copper free click reaction. References [1] Muñoz-Espin, D.; Serrano, M. Nat. Rev. Mol. Cell. Biol. 2014, 15, [2] Lee, H. W.; Heo, C. H.; Sen, D.; Byun, H. O.; Kwak, I. H.; Yoon, G.; Kim, H. M. Anal. Chem. 2014, 86, [3] Georgakopoulou EA., Tsimaratou K. Evangelou K., Fernandez Marcos PJ., Zoumpourlis V.,Trougakos IP., Kletsas D., Bartek J., Serrano M., and Gorgoulis VG., Aging, 2013, 5, 1. 4] J. A. Prescher, C.R. Bertozzi. Nat. Chem. Biol. 2005, 1, [5] H. W. Shih, D. N. Kamber, J. A. Prescher, Curr. Opin. Chem. Biol. 2014, 21,

88 P-43 URINARY METABOLOMIC FINGERPRINT AS BIOMARKER FOR MONITORING RECURRENCE IN NON-INVASIVE BLADDER CANCER Alba Loras 1, Guillermo Quintás 2,3, Marta Trassierra 4, Leopoldo Marzullo 4, Laura Lozano 4, Francisco Boronat 4, José Luis Ruiz 1,4. 1 Unidad Mixta de Investigación en Nanomedicina y Sensores. Instituto de Investigación Sanitaria La Fe, Universitat Politècnica de València. Valencia, Spain albaloras@gmail.com 2 Unidad Analítica, Instituto de Investigación Sanitaria La Fe, Valencia, Spain 3 Leitat Technological Center, Bio in vitro Division, Valencia, Spain 4 Servicio de Urología, Hospital Universitario y Politécnico La Fe, Valencia, Spain 3 keywords: Bladder cancer, metabolomics, biomarker INTRODUCTION: Metabolomics is a power tool for oncologic research. It studies the metabolites present in biological samples and identifies footprints related with tumor development. Ultra performance liquid chromatography - mass spectrometer (UPLC-MS) has been used in several studies for identify biomarkers [1]. OBJETIVES: Identify a urinary metabolomic footprint associated with non-invasive bladder tumor and analyze its changes after tumor resection (TUR) using UPLC-MS. SUBJECTS AND METHODS: 141 urines from 67 tumors were used. From each patient two samples were collected (pre-tur tumor and post-tur non-tumor ). Urines were analyzed by UPLC-MS to obtain the metabolomic profile. Data were pre-processed and then we performed a statistic analysis type PLS-DA (Partial Least Squares-Discriminant Analysis). We split up all samples into two sets: calibration (48 samples: 24 pre-tur and 24 post-tur), and validation (93 samples: 50 pre-tur y 43 post-tur. The model statistical significance was estimated by cross-validation and permutations test. The diagnostic safety of the validation group was adjusted to the probability of recurrence of the EORTC risk groups. RESULTS: The PLS-DA model showed a significantly different metabolic profile (p <0.02) between the pre-tur versus post-tur samples that were not attributable to chance or to model overfitting. The showed a sensibility of 67%, a specificity of 79%, a NPV of 70% and a PPV of 77%. A subsequent selection of the 108 most discriminating metabolic variables improved the statistics results: sensitivity of 84%, specificity of 70%, NPV = 79% and PPV = 76%. However, the analysis adjusted for probability of recurrence showed a NPV> 90% for the group of low, low-intermediate and high- intermediate risk. CONCLUSION: Although these results are preliminary, they enhance metabolomic research in the development of new biomarkers for the monitoring of CaV recurrence and could reduce cystoscopies in patients with negative results. [2] Bujak R, Struck-Lewicka W, Markuszewski MJ, Kaliszan R. J Pharm Biomed Anal. 2015, 113.,

89 P-44 EFFECT OF TEMPERATURE ON THE CORROSION CURRENTS J.R. Lliso-Ferrando 1, A. Martinez-Ibernón 1, J.E. Ramon 1,I. Gasch 1, R. Bataller 1 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain. Registered author jollife2@arq.upv.es Keywords: Temperature, Corrosion, Sensor The corrosion via macrocell effect is one of the most concerning processes regarding the deterioration of the steel embedded in concrete [1]. Therefore, many currently studies about the durability of structures are focused on this topic. Most of these studies are referred to the main macrocell precursors in reinforced concrete structures which are concrete carbonation and chloride attack. However, these are not the only two causes that affect the macrocell processes, but the increase of temperatures can also intensify them. The high temperatures provide the kinetic of the reaction. In addition, in the case of reinforced concrete structures, thermal expansion enhances the oxygen diffusion through the matrix. Both, temperature and dilatation, increase the corrosion rate. Whereas, the high temperatures produce an increase in evaporated interstitial water, thus, the electrical resistance of the concrete rise. According to all of this, the main goal of thus study is to analyse the effect of the temperatures in the corrosion current. In order to do this, a beam whit several specimens of corrugated black steel was manufactured. This beam was designed whit the purpose of getting different sunlight exposure hours, owing to this, a temperature gradient is produced throughout its length. Corrosion currents were obtained with the voltametric pulse method, developed by Alcañiz. M. [2]. References [1] Andrade, C., Maribona, I. R., Feliu, S., González, J. A., & Feliu, S. The effect of macrocells between active and passive areas of steel reinforcements. Corrosion Science, 1992, vol 33, no. 2, pp [2] Alcañiz M., Bataller R., Gandía-Romero J.M, Ramón J.E., Soto J., Valcuende M., Universitat Politècnica de València, Sensor, red de sensores, método y programa informático para determinar la corrosión en una estructura de hormigón armado, nºes (Patente de Invención concedida con Examen Previo) mayo 06,

90 P-45 IDENTIFICACIÓN TEMPRANA DEL FENÓMENO DE CONGELACIÓN EN NARANJAS (NAVEL-LATE) Nicolás Laguarda-Miró 1, Marta Muñoz-Alberto 1, Emma Serrano-Pallicer 1 1 Universitat Politècnica de València, Camí de Vera S/N, Valencia (Spain), nilami@iqn.upv.es 3 palabras clave: cítricos, electroscopía de impedancia, PCA Los cítricos son uno de los frutos más comercializados a nivel mundial con una producción total de más de 124 mill de toneladas en 2016 de las cuales casi 67 mill corresponden a producción de Naranjas. España, con una producción total de 6,88 mill de toneladas y 3,64 mill de toneladas de naranjas, se sitúa en quinta posición en términos de producción global [1]. En el marco actual referente al cultivo y comercialización de los cítricos existe un problema relacionado con la congelación de los mismos ante fenómenos meteorológicos de frío intenso. Hasta el momento, no se tiene conocimiento del desarrollo de ninguna técnica para poder diagnosticar la existencia de esta problemática de manera temprana en dichos frutos todavía en el árbol. En este trabajo se presenta la posibilidad de determinar, en las primeras 24 horas, si una naranja ha cambiado su respuesta electroquímica debido a haber sufrido fenómenos de heladas. Estos resultados se han logrado mediante la utilización de la técnica de espectroscopía de impedancias aplicada sobre las muestras utilizando un sensor específico. Una vez obtenidos los resultados de los ensayos, se procesan mediante Análisis de Componentes Principales (PCA) con el objetivo de reconocer de entre las muestras aquellas hayan sufrido heladas. A continuación, se muestra (Figura 1) una PCA con resultados experimentales, dónde se puede observar la diferencia en la respuesta electroquímica entre las muestras antes y después del fenómeno de heladas. Figura1.- PCA de los resultados de los ensayos de Espectroscopía de Impedancias. Referencias [1] FAO. Citrus Fruit Fresh And Processed Statistical Bulletin Food And Agriculture Organization Of The United Nations, Rome,

91 P-46 DESARROLLO DE MICROARRAYS PARA LA DETERMINACIÓN DE IgE ESPECÍFICA PARA AZTREONAM Mª José Juárez 1, Julia Oto Martínez 2, Ethel Ibáñez-Echevarría 2 Dolores Hernández Fernández de Rojas 2, Ángel Maquieira 1,3 Sergi Morais 1,3 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de Valencia, Universitat de Valencia, Camino de Vera s/n, 46022, Valencia, España. 2 Hospital Universitari i Politènic La Fe, Servicio de Alergología, Avinguda de Fernando Abril Martorell, 106, València, Spain 3 Departamento Química, Universitat Politècnica de València, Camino de Vera s/n Valencia, España. majuarod@upvnet.upv.es Alergia, inmunoensayo, monobactámicos La alergia a medicamentos se manifiesta como una respuesta anómala del organismo frente a un fármaco, consistente en muchas ocasiones en una reacción de tipo inmediato, desencadenada por la presencia de inmunoglobulina E (IgE) específica. Los antibióticos β-lactámicos (penicilina y derivados) son la causa más frecuente de este tipo de reacciones alérgicas a medicamentos [1,2]. El problema principal en el diagnóstico de alergia a medicamentos es la falta de pruebas diagnósticas sensibles y específicas que ayuden a confirmar las sospechas clínicas. Hoy en día, se comercializan pruebas de diagnóstico in vitro para la detección de alergia a cinco antibióticos β-lactámicos. Aztreonam es un antibiótico β-lactámico de la familia de las monobactamas. El hecho de que el anillo β-lactámico no esté condensado al anillo tiazol, hace de aztreonam un antibiótico que no produce reacción de hipersensibilidad cruzada con penicilinas y cefalosporinas, 1 por lo que puede ser segura su administración a pacientes alérgicos a estos antibióticos. Así pues, es interesante desde el punto de vista clínico y científico, desarrollar métodos de diagnóstico que permitan detectar y determinar IgEs específicas de aztreonam ya que puede utilizarse en pacientes alérgicos en sustitución a los antibióticos más habitualmente usados como amoxicilina y penicilina. Este trabajo presenta el desarrollo de una prueba diagnóstica de alta sensibilidad y especifica basada en un microarray para la determinación de IgE específica de aztreonam en suero sanguíneo. Se muestran los resultados obtenidos al comparar diferentes formatos de ensayo (reverso y directo), el efecto de los componentes del suero humano en las prestaciones analíticas del ensayo, así como la reactividad cruzada con otros antibióticos β-lactámicos. Referencia: [1] AHFS DRUG INFORMATION 2006 (2006 ed.). American Society of Health-System Pharmacists [2] Drug Allergy. EAACI White Book 2018, p Agradecimientos: Este trabajo ha sido financiado por el programa H2020 (proyecto COBIOPHAD ), siendo una iniciativa de la Asociación Fotónica Pública Privada ( 91

92 P-47 JANUS GOLD NANOSTARS-MESOPOROUS SILICA NANOPARTICLES FOR NIR LIGHT-TRIGGERED DRUG DELIVERY BY PHOTODISSOCIATION OF 2-NITROBENZYL DERIVATIVE Andy Hernández Montoto, 1 Antoni Llopis-Lorente, 1,3 Mónica Gorbe, 1,4 José Manuel Terrés, 1 Roberto Montes, 1 Roberto Cao-Milán, 5 Borja Díaz de Greñu, 1,3 María Alfonso, 1,3 María Dolores Marcos, 1,2,3,4 Mar Orzáez, 4 Reynaldo Villalonga, 6 Ramón Martínez-Máñez, 1,2,3,4 Félix Sancenón 1,2,3,4 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València (Spain), ahm870809@gmail.com 2 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia (Spain). 3 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) (Spain). 4 Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Valencia, Universitat Politècnica de València, Centro de Investigación Príncipe Felipe, València, Spain. 5 Facultad de Química, Universidad de la Habana, La Habana (Cuba). 6 Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Madrid (Spain). 3 keywords: drug delivery, NIR light, Janus nanoparticles Here, we present a novel drug delivery system responsive to near infrared (NIR) light based on the photocleavage of 2-nitrobenzyl derivative. A photolabile molecule with thiol group was synthesized from vanillin and used for the preparation of a nanodevice with peculiar architecture and release mechanism. The system consists of novel Janus gold nanostars-mesoporous silica nanoparticles (AuNSt-MSNP). The AuNSt face is functionalized with photolabile molecules, whereas the silica face is loaded with doxorubicin and capped with proton-responsive supramolecular nanovalves. Photolabile compound, attached to the gold face, releases succinic acid upon NIR-light irradiation, which induces the opening of the nanovalve and cargo delivery from the mesoporous face. The hybrid nanoparticles show no cytotoxicity toward HeLa cells, until they were irradiated with a NIR laser, leading to intracellular doxorubicin release. This nanodevice is an excellent platform for NIR light triggered drug delivery in cancer therapy. 92

93 P-48 NEAR INFRARED LIGHT TRIGGERED PHOTOACTIVATION OF DOXORUBICIN PRODRUGS BY MULTIPHOTON MOLECULAR DISSOCIATION OF 2-NITROBENZYL PHOTOLABILE LINKER USING GOLD NANOSTARS Mónica Gorbe, 1,4 Andy Hernández Montoto, 1 José Manuel Terrés, 1 Roberto Montes, 1 Roberto Cao-Milán, 5 Borja Díaz de Greñu, 1,3 María Alfonso, 1,3 María Dolores Marcos, 1,2,3,4 Mar Orzáez, 4 Félix Sancenón, 1,2,3,4 Ramón Martínez-Máñez 1,2,3,4 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València (Spain), mogormo@upvnet.upv.es 2 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia (Spain). 3 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) (Spain). 4 Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Valencia, Universitat Politècnica de València, Centro de Investigación Príncipe Felipe, València, Spain. 5 Facultad de Química, Universidad de la Habana, La Habana (Cuba). 3 keywords: doxorubicin prodrugs, multiphoton molecular dissociation, gold nanostars In this work, a novel mechanism for photoactivation of two doxorubicin prodrugs based on near infrared (NIR) light triggered multiphoton molecular dissociation of 2-nitrobenzyl linker using gold nanostars (AuNSts), was reported. In this scenario, a prodrug (1) was synthesized by modification of doxorubicin (Dox) with 4,5-dimethoxy-2-nitrobenzyl alcohol via carbamate linkage. NIR irradiation of this photolabile compound in presence of AuNSts produces photocleavage of 2-nitrobenzyl moiety and doxorubicin delivery. Irradiation of gold nanostars, whose localized surface plasmons can be excited in NIR region, leads to strong electromagnetic field enhancement on their sharp tips and favors multiphoton absorption and photocleavage of molecules near to nanoparticle s surface. Another prodrug (2), which can attach directly to AuNSts surface, was obtained by modification of doxorubicin with a 2-nitrobenzyl diol bearing a disulphide group. AuNSts were modified with the prodrug 2 obtaining AuNSt@(PLM-Dox) 2 nanoparticles. Also, they were irradiated with NIR laser producing multiphoton molecular dissociation of 2- nitrobenzyl linker and doxorubicin release from nanoparticles. Both prodrugs showed a greatly reduced cytotoxicity toward HeLa cells when cells were co-incubated with the prodrug 1 and AuNSts or incubated with AuNSt@(PLM-Dox) 2 nanoparticles and were irradiated with 808 nm laser, provoking photodissociation of both prodrugs and intracellular doxorubicin delivery. Intracellular doxorubicin delivery was followed with confocal microscopy. 93

94 P-49 Mono-functionalization of TiO 2 nanoparticles and attachment to DNA probes for biosensing Daniel González-Lucas 1, María-José Bañuls 1,2, Rosa Puchades 1,2, Ángel Maquieira 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain. (dagonlu@upvnet.upv.es). 2 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain keywords: TiO 2 nanoparticles, molecular beacons, streptavidin Titanium nanoparticles have been widely studied in the past. [1,2] A very interesting approach is exploiting their reactivity with catechol derivatives. [3,4] In this work, a procedure for the single functionalization of TiO 2 nanoparticles was optimized to obtain a 1:1 ratio catechol:nanoparticle when using 2 nm TiO 2 particles. HO R HO O R O Figure 1: Nanoparticle functionalization with catechol derivatives (R = -COOH, -(CH 2 )NH 3 ). The functionalized nanoparticles were characterized and attached to modified molecular beacon (MB) sequences, which are DNA probes with a hairpin conformation. The ability of the MB-NP complex to recognize its complementary strand, was studied in microarray format and its conformational changes upon hybridization are being studied using quartz crystal microbalance (QCM). Acknowledgement: This work was financed from the Horizon 2020 European Union programme (ICT , SAPHELY project), MINECO project CTQ/2016/75749-R, FEDER and GVA PROMETEO II 2014/40. References: [1] Paunesku, T.; Rajh, T.; Wiederrecht, G.; Maser, J.; Vogt, S.; Stojicevic, N.; Protic, M.; Lai, B.; Oryhon, J.; Thurnauer, M.; Woloschak, G., Nat Mater 2003, 2, [2] Paunesku, T.; Vogt, S.; Lai, B.; Maser, J.; Stojićević, N.; Thurn, K. T.; Osipo, C.; Liu, H.; Legnini, D.; Wang, Z.; Lee, C.; Woloschak, G. E., Nano Lett. 2007, 7 (3), [3] I. A. Janković, Z. V. Šaponjić, E. S. Džunuzović, J. M. Nedeljković, Nanoscale Res Lett 2010, 5, [4] M. S. Ata, Y. Liu, I. Zhitomirsky, RSC Advances 2014, 4,

95 P-50 Formación de nuevos polímeros supramoleculares mediante enlace de halógeno inducidos por aniones Lidia González 1, Fabiola Zapata 1, Antonio Caballero 1 *, Adolfo Bastida 2, Delia Bautista 3, Pedro Molina 1 *. 1 Dpto de Química Orgánica, Universidad de Murcia, Campus de Espinardo Murcia, España, lidia.gonzalez@um.es 2 Dpto de Química Física, Universidad de Murcia, Campus de Espinardo Murcia, España 3 Servicio de Apoyo a la investigación, Universidad de Murcia, Campus de Espinardo Murcia, España Polímeros Supramoleculares, Enlace de Halógeno La obtención de polímeros supramoleculares inducidos por aniones es un campo prácticamente inexplorado debido fundamentalmente a la tendencia de los receptores tradicionales de aniones a formar moléculas discretas en el proceso de reconocimiento.[1] Debido a las características intrínsecas del enlace de halógeno, nos planteamos en este trabajo su utilización para la formación de estructuras supramoleculares autoenlazadas. Para ello se ha diseñado un nuevo receptor bidentado de aniones basado en la unidad de iodo-triazolio como unidad de reconocimiento de aniones por enlace de halógeno (Figura 1a). Experimentos realizados mediante 1 H-RMN, DOSY-RMN, DLS, SEM y Rayos X demuestran la capacidad del receptor para formar polímeros supramoleculares 2- con la presencia de SO 4 y H 2 PO - 4. Los polímeros supramoleculares obtenidos - presentan una organización vesicular o de micela en estado sólido para el anión H 2 PO 4 2- (Figura 1b), y fractales para el anión SO 4 (Figura 1c). Figura 1. a) Estructura del nuevo receptor 1 basado en iodo-triazolios. Imágenes SEM del polímero supramolecular b) H 2 PO 4 - y c) 1 2+ SO Agradecemos al Ministerio de Economía y Competitividad de España y proyecto FEDER CTQ P y CTQ P, a la Fundación Séneca Región de Murcia (CARM) proyecto 18948/JLI/13 y 19337/PI/14. L. González agradece al Ministerio de Economía y Competitividad de España la concesión de un contrato del programa FPI. Referencias [1] F. Zapata, L. González, A. Caballero, A. Bastida, D. Bautista, P. Molina, J. Am. Chem. Soc., 2018, 140,

96 P-51 PHOTOCATALYTIC ACTIVITY OF SEMICONDUCTOR NANOMATERIALS FOR WATER TREATMENT Soranyel Gonzalez-Carrero 1, Francisco J. Valverde 1, Willber D. Castro- Godoy 1 José A. Real 1, Raquel E. Galian 1 and Julia Pérez-Prieto 1, 1 Instituto de Ciencia Molecular (ICMol), Universidad de Valencia, Catedrático José Beltrán 2, 46980, Paterna, Valencia, Spain soranyel@uv.es keywords: heterogeneous photocatalysis, semiconductor nanomaterial, organic pollutant The purification of water is one of the challenges of current society due to the increase of polluting substances such as dyes, pesticides, pharmaceuticals, among others. Heterogeneous photocatalysis is one of the most used methods for water treatment, based on the use of wide-band-gap semiconductor materials and UV VIS irradiation to decompose organic or inorganic compounds present in water environment. Although titanium oxide (TiO 2 ) is widely considered as one of the most effective semiconductor photocatalyst, it can only be excited by UV-light. Therefore, several efforts have been made to obtain materials active in the visible light for heterogeneous photocatalytic processes. [1] Herein, we present the photocatalytic activity of different semiconductor nanomaterials, such as magnetite (Fe 3 O 4 ), cadmium sulfide (CdS) and metal organic framework (MOF- Fe) for the degradation of two models of organic pollutants, in particular methylene blue (MB) and methyl orange (MO), under visible light irradiation. [2] The effect on the outcome of different parameters, such as irradiation time, dye concentration and catalyst loading was investigated. The degradation percent of model dyes in water was monitored following the characteristic absorption bands of the dye in the UV-Visible absorption spectrum. Additionally, experiments using direct solar radiation as the irradiation source were also carried out. Interesting, a remarkable percentage of MB degradation in water was observed in the presence of MOF-Fe, indicating it is an excellent candidate for water treatment under visible light. The recyclability of MOF-Fe material over several photocatalytic cycles with successful regeneration of the material and the mechanism of organic dyes degradation will be discussed. References [1] Malato, S.; Maldonado, M. I.; Fernández-Ibáñez, P.; Oller, I.; Polo, I.; Sánchez- Moreno, R. Materials Science in Semiconductor Processing 2016, 42, [2] Gándara, F.; García-Cortés, A.; Cascales, C.; Gómez-Lor, B.; Gutiérrez-Puebla, E.; Iglesias, M.; Monge, A.; Snejko, N. Inorganic Chemistry 2007, 46,

97 P-52 PREDICCIÓN DEL CONTENIDO DE CLORURO DE SODIO, NITRITO SÓDICO Y NITRATO POTÁSICO EN CARNE PICADA Y SALMUERA MEDIANTE UNA LENGUA ELECTRÓNICA POTENCIOMÉTRICA Luis Gil Sánchez 1, Diana Baigts 2, José Manuel Barat 2 1 Instituto Interuniversitario de Reconocimiento Molecular y Desarrollo Tecnológico. Universitat Politècnica de València, lgil@eln.upv. 2 Departamento de Tecnología de Alimentos, Universitat Politècnica de València Lengua electrónica potenciométrica, sales, nitritos, nitratos Estas sales son muy utilizadas en la producción de productos cárnicos curados debido a sus propiedades funcionales (agentes curantes). Un estudio de detección de estas sustancias ya se había realizado utilizando técnicas voltamétricas e impediométricas [1-2]. Equipo medida: conjunto de electrodos metálicos de distinto material (AgCl, CuS, Ag, Au, Cu, AgBr). Estudio previo: viabilidad de usar sensores metálicos sometidos a oxidación para aumentar el número de respuesta potencial. Tres niveles de adicción de sal: bajo (0), medio (125 ppm nitritos, 1,5% NaCl), alto (250 ppm nitritos, 3% NaCl). Se tomaron 18 muestras de disolución y de carne de cerdo picada a las que se añadieron sales con niveles de cada componente en las diferentes combinaciones establecidos por medio de un diseño experimental MODDE 8. Resultados: medida del potencial de cada electrodo metálico respecto a electrodo de Ag- ClAg mediante sistema electrónico de precisión de alta impedancia. En las señales obtenidas tanto en salmuera y carne hubo diferencia de voltaje entre la ausencia y presencia de sal pero poca entre muestras con sal. Los electrodos con mayor sensibilidad a este compuesto fueron AgC y AgBr (-0,17 0,15V). El análisis PCA permitió una clara agrupación de muestras con NaCl y sin ella y, dentro del grupo de sin sal sobresale un subgrupo con alta concentración de NaNO. En cuanto al peso de cada electrodo en este análisis los electrodos de oro y plata poseen un comportamiento diferente al resto que tiene un comportamiento similar entre ellos. La predicción se realizó utilizando redes neuronales artificiales. Lógicamente la mejor tasa de acierto se obtuvo en la discriminación de NaCl (96,87%). Para las sales nitrificantes el éxito fue bajo (sobre 60%) tomando en cuenta que la proporción de NaCl presente en las muestras era mucho mayor. También se realizaron análisis de predicción PLS y mapas SOM con resultados similares a las redes neuronales. Como conclusión general se puede indicar que la presencia de NaCl enmascara la presencia de los otros dos componentes. Referencias [1] R. Labrador, R. Masot, M. Alcañiz, D. Baigts, J. Soto, R. Martínez-Mañez, E. García-Breijo, L. Gil, J. M. Barat. Food Chemistry, , [2] I. Campos, R. Masot, M. Alcañiz, L. Gil, J. Soto, J.L. Vivancos, E. García-Breijo, R. Labrador, J.M. Barat, R. Martínez-Mañez, Sensors and Actuators B. Chemical, ,

98 P-53 Antibody capped mesoporous silica nanoparticles for the selective fluorogenic detection of psychedelic drug 25I-NBOMe Eva Garrido, 1,3,4 Maria Alfonso, 1,3,4 Borja Díaz de Greñu, 1,2,3,4 M. Dolores Marcos, 1,2,3,4 Ramón Martínez-Máñez 1,2,3,4 and Félix Sancenón 1,2,3,4 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Spain. evgarga5@upvnet.upv.es 2 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). 3 Unidad Mixta de Investigación en Nanomedicina y Sensores. Universitat Politècnica de València, Instituto de Investigación Sanitaria La Fe, Valencia, Spain. 4 Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Valencia, Universitat Politècnica de València, Centro de Investigación Príncipe Felipe, Valencia, Spain. 3 keywords: 25I-NBOMe, 5-HT 2A receptor, chromo-fluorogenic detection The consumption of new psychoactive substances has increased exponentially becoming a worldwide problem that concerns to governments and international institutions. According to the last report published by UNODC [1] and EMCDDA [2], around 5 % of the global adult population used drugs at least once in Recently, a new synthetic family of chemicals known as NBOMe (N-benzyl-oxy-methyl derivatives of 2C phenylethylamines) has emerged as potent abuse drugs. NBOMe is a family of serotonergic psychedelic drugs which act as extremely potent and highly selective agonists of the serotonin 5-HT 2A receptor. [3] These chemicals derived from the 2C family receive different names such as "N-Bomb", "Solaris", "Smiles", or "Wizard". One the most remarkable components inside the NBOMe family is 25I- NBOMe (Figure 1). It is a psychedelic hallucinogen commonly used in biochemistry research that has also been used for recreational purpose recently. This illicit drug produces potent effects as euphoria, muscle tension, nausea, anxiety, paranoia, memory suppression, hallucinations and empathy enhancement among others. In this scenario, the design of simple to use chromofluorogenic probes for selective and sensitive 25I-NBOMe detection is highly appealing. Bearing these facts in mind and our interest in the development of gated hybrid materials for their application in new sensing protocols, [4] herein we report the synthesis and characterization of mesoporous silica nanoparticles (MSNs) loaded with rhodamine B and functionalized on the external surface with a serotonin derivative which interacts with 5-HT 2A receptor antibody, acting as a molecular gate that will open in presence of 25I-NBOMe producing a chromo-fluorogenic signal. References [1] World drug report. United Nations Office on Drugs and Crime. (UNODC). Inform [2] European drug report: Trends and Developments. European Monitoring Centre for Drugs and Drug Addiction (EMCDDA). Inform [3] Rickli, A.; Luethi, D.; Reinisch, J.; Buchy, D.; Hoener, M. C.; M. E. Liechti. Neuropharmacology. 2015, 99, [4] (a) L. Pascual, S. El Sayed, R. Martinez-Mañez, A. M. Costero, S. Gil, P. Gaviña and F. Sancenon. Org. Lett. 2016, 18, ; (b) S. El Sayed, M. Milani, M. Licchelli, R. Martinez-Mañez, F. Sancenon. Chem. Eur. J. 2015, 21, ; (c) E. Climent, L. Mondragon, R. Martinez-Mañez, F. Sancenon, M. D. Marcos, J. R. Murguia, P. Amoros, K. Rurack, E. Pérez-Payá. Angew. Chem., Int. Ed. 2013, 52, H 3 C O I O CH3 H N O CH3 Figure 1. Structure of 25I-NBOMe.

99 P-54 Study of internalization pathways of mesoporous silica nanoparticles in human breast cancer cells Iris Garrido-Cano 1,2, Vicente Candela-Noguera 2, Guadalupe Herrera 1, Ana Lluch 1,3,4, Ramón Martínez-Máñez 2,5, Pilar Eroles 1,4 1 INCLIVA Biomedical Research Institute, Valencia. irgarca@dctor.upv.es 2 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Valencia. 3 Universidad de Valencia, Valencia. 4 CIBER de Oncología (CIBERONC). 5 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). KEYWORDS: Mesoporous Silica Nanoparticles, endocytosis Mesoporous silica nanoparticles (MSNs) are biocompatible solid materials with a porous structure composed by hundreds of channels (mesopores). They have unique properties such as high surface area and pore size, as well as remarkable chemical and thermal stability. Moreover, MSNs can be functionalized with certain molecules and/or supramolecules, which can act as molecular gates and control the delivery of stored molecules inside the MSNs pores 1. Because of its peculiar features, MSNs are being studied for multiple biomedical applications such as medical imaging, diagnostic, biosensors, or controlled drug delivery 2. Our aim was to study the mechanisms by which MSNs are internalized by cells and how functionalization of nanoparticles influence on it. To achieve it, we synthesized rhodamine-conjugated MSNs (MSN-rh), and functionalized them with hyaluronic acid (HA- MSN-rh) or polyethylene glycol (PEG-MSN-rh). Then, we used different internalization inhibitors and compared the percentage of internalizing cells by flow cytometry. The results were confirmed by confocal microscopy. In this way, it would be possible to assess the endocytic mechanism of MSN-rh, HA-MSN-rh and PEG-MSN-rh. [1] Wang, Y. et al., Nanomedicine: NBM 2015;11: [2] Mamaeva, V., Sahl, C. & Lindén, M. Adv. Drug Deliv. Rev. 2013;65:

100 P-55 Image analysis for quality control of table olives during storage Cristina Fuentes, Samuel Verdú, Ana Fuentes, Raúl Grau, José Manuel Barat Department of Food Technology, Universitat Politècnica de València. Camino de Vera s/n, 46022, Valencia, Spain, Table olives, image analysis, quality control Colour is a quality attribute of great importance for table olives. Chlorophylls and carotenoids are the main pigments responsible for the colour of green olives [1]; however, browning reactions could occur during packing operations and/or during storage time, affecting the quality and product acceptance. Table olives quality is evaluated by sensory panels, but this type of assessment has several drawbacks, such as taster subjectivity, variability of responses over time, lack of reference standards, time consuming, and low number of analyses per day [2]. For this reason, food industry search for alternatives to human sensory panels based on instrumental methods. Recent advances in image analysis have led to the development of new non-invasive systems applicable to food control with the minimum of human intervention. The objective of this study was to apply image analysis technique to evaluate the quality of pitted table olives packaged in plastic pouches during 90 days of storage at room temperature and 50 ºC. Through the storage period, olives were analysed in order to determine brine colour by measuring the difference in absorbance at 440 and 700 nm, and the visual quality of the samples using a sensory trained panel. In addition, images of the olives pouches were periodically recorded with the Logitech HD Pro Webcam C920. Image acquisition was performed in reflectance and transmittance modes. During storage, brine colour of the samples changed significantly, indicating an evident brine darkening. A significant decrease in the overall liking scores given by the panellist to the samples was also observed. Partial least squares-discriminant analysis models obtained from image analysis data were able to detect and predict the overall acceptance of the samples. The results obtained in this study indicate that image analysis could be employed as a novel classification approach to obtained qualitative information about visual table olives quality with minimum human intervention. References [1] Rojas-Gandul, B., Gallardo-Guerrero, L. Food Research International. 2018, 108, [2] Veloso, A.C.A, Silva, M., Rodrigues, N., Rebello, L.P.G., Dias, L.G., Pereira, J.A., Peres, A.M. Talanta. 2018, 176,

101 P-56 Toxicity evaluation of vanillin functionalised silica particles used as antimicrobial agents Cristina Fuentes 1, María Ruiz-Rico 1, Ana Fuentes 1, Ana Juan-García 2, María José Ruiz 2, José Manuel Barat 1 1 Department of Food Technology, Universitat Politècnica de València. Camino de Vera s/n, 46022, Valencia, Spain, crifuelp@upvnet.upv.es 2 Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, Burjassot, València, Spain Silica particles, vanillin, toxicity, antimicrobial agents In the last years, consumer awareness on food safety has forced food industry to investigate different alternatives to chemical additives. Plant-derived compounds have attracted considerable attention due to their well-known antimicrobial activity against a wide range of bacteria and fungi. However, its application to food products presents different problems such as high volatility, low water solubility or strong flavour and odour, among others. For this reason, the immobilisation of natural bioactive compounds on silica supports has been developed as a novel strategy to enhance their antimicrobial activity and avoid drawbacks. Although the efficacy of these devices against spoilage microorganisms and food-borne pathogens has been demonstrated [1, 2], their safety is still under research. The aim of the present work was to evaluate the potential toxicity of silica supports designed to be applied as antimicrobial systems in the food industry. For this purpose, the in vitro toxicity of free vanillin and vanillin functionalised silica particles was evaluated. The phenolic derivative was immobilised on the surface of three different silica particles: amorphous silica, MCM-41 microparticles and MCM-41 nanoparticles. The results obtained during the material characterization indicated the immobilisation was correct and did not affect the integrity of the particles. The EC50 for vanillin was 2554 ± 7 and 2509 ± 144 µm for 24 and 48 h, respectively. The results obtained suggest that in vitro toxicity of immobilised vanillin supports was lower than the free compound toxicity. The development of toxicological studies on these new antimicrobial systems provides essential information to identify possible hazards associated to their applications, and therefore to ensure their safety for human health. References [1] S. Ribes, M. Ruiz-Rico, É. Pérez-Esteve, A. Fuentes, P. Talens, R. Martínez-Mañez, J.M. Barat. Food Control. 2017, 81, [2] M. Ruiz-Rico, É. Pérez-Esteve, A. Bernardos, F. Sancenón, R. Martínez-Mañez, M. Marcos, J.M. Barat. Food Chemistry. 2017, 233,

102 P-57 Singlet oxygen detection by sensitization from upconversion nanoparticles to xanthene dyes Juan Ferrera-González 1, Nestor Estébanez-Bloem 1, Laura Francés-Soriano 1,2, María González-Béjar 1, Julia Pérez-Prieto 1 1 Instituto de Ciencia Molecular (ICMol)/ Departamento de Química Orgánica, University of Valencia, Catedrático José Beltrán 2, 46980, Paterna, Valencia, Spain. 1,2 Institut de Biologie Intégrative de la Cellule (I2BC), Bâtiment 21, Avenue de la Terrasse, Gif-Sur-Yvette, France Upconversion nanoparticles, Förster Resonane Energy Transfer, singlet oxygen Upconversion lanthanide-based nanoparticles (UCNPs) are promising nanosystems for biomedical purposes. They absorb photons of low energy (NIR light) and emit photons of higher energy (in the UV-visible-NIR range) due to the cooperation of different lanthanides ions. When combining the emission properties of the UCNPs with appropriate absorption of organic dyes, these systems become ideal for photodynamic therapy (PDT) [1]. In this way, the singlet oxygen production has been tested by NIR-excitation of UCNPs capped with bengal rose (a xanthene dye which is a very good singlet oxygen generator) [2]. The nanohybrid was synthesized using ligand-free β-nayf 4 : Yb(20%),Er (2%) UCNPs, whose green emission fits perfectly with the absorption spectrum of the dye (Figure 1), thus allowing the Resonant Energy Transfer from Er 3+ ions to the organic dye [3] and eventually to molecular oxygen. The formation of singlet oxygen was indirectly detected by using a fluorescent probe, specifically 9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA), in an oxygen-saturated solution of phosphate buffered saline (PBS) in deuterated water [4]. Figure 2. Emission spectrum of the UCNP (black line) and bengal rose absorption spectrum (red surface) in PBS. References [1] Wang F, Banerjee D, Liu Y, Chen X, Liu X. Analyst. 2010;135(8):1839. [2] DeRosa M, Crutchley RJ. Coordination Chemistry Reviews. 2002; : [3] Wang Y, Liu K, Liu X, Dohnalová K, Gregorkiewicz T, Kong X et al. The Journal of Physical Chemistry Letters. 2011;2(17): [4] González-Béjar M, Liras M, Francés-Soriano L, Voliani V, Herranz-Pérez V, Duran-Moreno M et al. J Mater Chem B. 2014;2(28):

103 P-58 Electrocatalytic effect on sensors based on carbon foams modified with phthalocyanines C. Fernandez-Blanco 1, C. Garcia-Cabezon 2, M.A. Rodríguez-Pérez 3, M. L. Rodriguez- Mendez 1 1 Group UVaSens, Engineers School, Universidad de Valladolid, Valladolid (Spain) anacristina.fernandez@uva.es 2 Departamento de Ingeniería de Materiales, Engineers School, Universidad de Valladolid, Valladolid, (Spain) 3 Group Cellmat, Faculty of Sciences, Universidad de Valladolid, Valladolid (Spain). 3 keywords: carbon foam, catechol, electrochemical sensor Electrochemical sensors have been widely employed for the antioxidants detection such as phenolic acids, flavonoids or cinnamic acids [1]. These sensors can be modified with different electrocatalytic and sensitive materials, such as metallic phthalocyanines, metallic nanoparticles, carbon nanotubes or enzymes for the determination of polyphenols compounds [2]. To improve the electrochemical activity for a sensor, new devices based on carbon foams are studied. Foams have been chosen due to the increase in their electroactive area and, therefore, the increase in the surface coverage of the redox species (Г). Both ideas give a better sensitivity for our sensors and better limit of detections. For that reason, we have studied the electrochemical behavior for new carbon foams modified with different phthalocyanines with sensing properties towards polyphenols with antioxidants properties. Moreover, the sensors can distinguish catechol and hydroquinone, two polyphenols that are isomers. Acknowledgments Financial support by MINECO-FEDER (AGL R) and the JCyL-FEDER (VA- 011U16) is gratefully acknowledged. C. Fernandez-Blanco thanks to JCyL-FEDER for a postdoctoral fellowship (VA-011U16). References [1] P. A. Kilmatin, H. L. Zou, A. L. W. American Journal of Enology and Viticulture. 2002, 53, [2] F. J. Pavinatto, E. G. R. Fernandes, P. Alessio, C. J. L. Constantino, J. A. de Saja, V. Zucolotto, C. Apetrei, O. N. Oliveira Jr, M. L. Rodriguez-Mendez. Journal of Materials Chemistry. 2011, 21,

104 P-59 COBIOPHAD project: Progress towards in vitro diagnosis of drug allergies by a point-of-care device Estrella Fernández 1, Teresa Molina 1, Sergi Morais 1, 2, Luís A. Tortajada 1, 2, Rosa Puchades 1, 2, Ángel Maquieira 1, 2. 1 Instituto Interuniversitario de reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València. Camino de Vera, s/n Valencia. esfersan@upv.es 2 Departamento de Química. Universitat Politècnica de València. Camino de Vera, s/n Valencia. in-vitro test allergies Over 2.5 million people in Europe suffer from hypersensitivity to Beta Lactam Antibiotics (BLCs). Moreover, BLC allergy is the most frequent cause (47%) of drug anaphylaxis [1], and is an important public health problem with an estimated in additional hospitalization costs of /patient. Furthermore, patients with multiple drug allergies are difficult to manage, leading to the use of alternative antibiotics that may be more expensive, less efficacious, and even have more side effects [2]. The diagnostic approach to immediate drug hypersensitivity reactions requires a detailed clinical history and other tests that may include in vivo procedures, such as skin tests (STs) and drug provocation tests (DPTs) and in vitro diagnostic (IVD) tests [1]. The in vivo tests are invasive and risky and they should be performed under the supervision of experienced personnel. In daily practice, few IVD tests are available and show low sensitivity, analyze as much as 5 BLCs and give false positive and negative rates. The development of IVD tests for the detection of specific IgEs associated to BLC hypersensitivity is highly demanded to substitute the invasive and risky in vivo tests. Consequently, COBIOPHAD (Compact biophotonic platform for drug allergy diagnosis) aims at the development of a competitive multiplexed diagnostic device to provide sensitive, selective, rapid and cost-effective IVD tests. The COBIOPHAD device takes advantage of the compact disc technology to fully automate the immunoassay. The consortium is developing a modified optical reader to manage the COBIOPHAD centrifugal microfluidic disc and read the microarray. Also, a sophisticated cloud-based data networking and management system will eventually provide allergy decision support to medical staff in clinics and hospitals worldwide. Acknowledges: The COBIOPHAD project has received funding from the European Union s Horizon 2020 research and innovation programme under grant agreement No It is an initiative of the Photonics PPP ( References [1] Blanca M, Romano a., Torres MJ, et al. Allergy. 2009, 64 (2) [2] David Khan, Aleena Banerji. Drug Allergy Testing. 1, Elsevier Inc, United States of America

105 P-60 STUDIES IN AQUEOUS MEDIA OF SELF-ASSEMBLED BIS- IMIDAZOLIUM SALTS BASED ON PSEUDOPEPTIDIC COMPOUNDS Ferran Esteve 1, A. Valls 1, B. Altava 1, M. I. Burguete 1, L. González 1, S. V. Luis 1, I. Peña 1 1 Departamento de Química Inorgánica y Orgánica, Escola Superior de Tecnologies i Ciències Experimentals, Universitat Jaume I, Av. Sos Baynat s/n, 12071, Castellón, estevef@uji.es 3 keywords: pseudopeptides, self-assembly, recognition Amino acids and their derivatives are used by nature as structural units for constructing more complex systems. This is because they contain a large functional density and are able to provide a significant structural variability given by their side chains. Natural peptides also play very important roles in catalytic systems and in molecular devices for energy, matter and information transport. Therefore, through the combination of a limited number of amino acids (peptides) and depending on their relative arrangement, a defined function can be achieved. Although huge molecules can be formed, sometimes only one specific peptidic segment is involved with the functionality of the whole peptidic structure[1]. The study of the self-assembling properties of organic salts, in particular imidazolium salts, has recently been a topic of great interest in numerous publications[2]. Moreover, their properties can be easily tuned by small changes in the cation or anion structure, obtaining materials with different applications of interest[3]. In this context, our research group has been involved in the preparation and study of different imidazolium receptors derived from amino acids[4], which are able to selectively recognize and transport chloride anions and carboxylates [4c]. In this project, we report the study of bis-imidazolium salts derived from natural amino acids and their capability to form aggregates in water mixtures involving a fast-dynamic equilibrium exchange. X X N N N N O NH HN O References [1] S. V. Luis, I. Alfonso, Acc. Chem. Res., 2014, 47, [2] a) V. A. Mallia, H. I. Seo, R. G. Weiss, Langmuir, 2013, 29, d) D. Wu, R. Liu, W. Pisula, X. Feng, K. Müllen, Angew. Chem., Int. Ed., 2011, 50, [3] M. Rodrigues, A. Yagüe, A. C. Calpena, D.B. Amabilino, J. González-Linares, M. Borrás, L. Pérez- García, Langmuir, 2012, 28, [4] a) B. Altava, D. S. Barbosa, M.I. Burguete, J. Escorihuela, S. V. Luis, Tetrahedron: Asymmetry 2009, 20, b) L. González, B. Altava, M.I. Burguete, R. Quesada, S.V. Luis, Org. Biomol. Chem., 2015, 13, c) L. González, B. Altava, M.I. Burguete, S.V. Luis, RSC Adv., 2015, 5,

106 P-61 MESOPOROUS SILICA NANOPARTICLES TO SELECTIVE TARGET ENDOTHELIAL SENESCENT CELLS Alejandra Estepa-Fernández 1, 2, María Alfonso 2,3,4 Félix Sancenón 2,3,4, Ramón Martínez- Máñez 1,2,3,4, Mar Orzáez 1,5 1 Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina. Valencia. Universitat Politècnica de València. Centro de Investigación Príncipe Felipe, Eduardo Primo Yúfera, 3. Valencia 46,012, Spain. aestepa@cipf.es 2 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universitat de València- Universitat Politècnica de València. Camino de Vera s/n, 46022, Valencia, Spain. 3 Departamento de Química. Universidad Politécnica de Valencia. 4 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Valencia, Spain 5 Centro de Investigación Príncipe Felipe, Eduardo Primo Yúfera, 3. Valencia 46,012, Spain Keywords: senescence, metastasis, endothelial cells Cellular senescence is a stable and long-term cell cycle arrest [1] that can be triggered in proliferating cells as consequence of a stress response. As senescent cells are cell cyclearrested, induction of senescence has been explored as a barrier against tumor progression and is emerging as a therapeutic concept [2]. To this end, senescenceinducing compounds have been developed, including CDK4/6 inhibitors such as palbociclib. In clinic, this drug is indicated for the treatment of patients with metastatic or locally advanced breast cancer hormonal receptor positive (HR + ) and human epidermal growth factor receptor 2 negative (HER2 - ) [3-5]. Regardless senescence inductors in clinic seem to be effective, the accumulation of senescent cells in tissues and organs is largely detrimental causing deleterious effects on tissue microenvironment including tumor promoting properties that may drive the formation of secondary tumors or cancer relapse [6-10]. It has been recently demonstrated that endothelial senescence causes alterations of the cytoskeleton and increases permeability facilitating transendothelial migration, promoting neutrophil infiltration, tumor cell adhesion and metastasis [11]. In this work, we have settled an in vitro model to study breast cancer cell migration induced by endothelial senescent cells. Our results demonstrate that treatment with palbociclib induces endothelial senescence increasing breast cancer cell migration. An effective strategy to overcome the negative side effects of senescent cell accumulation is to enhance senolysis. In this context, our group have previously reported gated mesoporous silica nanoparticles (MSNs) capable to selectively release their cargo in senescent cells with high specificity and lack of toxicity [12]. The use of MSNs load with senolytic to selective target senescent cells has been evaluated in a cellular model of endothelial senescence. There is a preferential accumulation of MSNs in senescent versus non-senescent endothelial cells, which evidences the potential of these nanoparticles as future treatments for prevention of long-term side effects caused by senescence drug induction. References [1] Campisi J et al. (2007) Cellular senescence: when bad things happen to good cells. Nat Rev Mol Cell Biol, 8(9): [2] Michaloglou C et al. (2005) BRAFE600-associated senescence-like cell cycle arrest of human naevi. Nature, 436(7051): [3] Finn RS et al. (2009) PD , a selective cyclin D kinase 4/6 inhibitor, preferentially inhibits proliferation of luminal estrogen receptor-positive human breast cancer cell lines in vitro. Breast Cancer Res, 11(5):R

107 [4] Turner NC et al. (2015) Palbociclib in Hormone-Receptor-Positive Advanced Breast Cancer. N Engl J Med, 373(3): [5] Finn RS et al. (2016) Palbociclib and Letrozole in Advanced Breast Cancer. N Engl J Med, 375(20): [6] Ruhland MK et al. (2016) Senescence and cancer: An evolving inflammatory paradox. Biochim Biophys Acta Jan;1865(1): [7] Coppé JP et al. (2010) The senescence-associated secretory phenotype: the dark side of tumor suppression. Annu Rev Pathol, 5: [8] Kang, T.W. et al. (2011) Senescence surveillance of pre-malignant hepatocytes limits liver cancer development. Nature 479, [9] Iannello, A. et al. (2013) p53-dependent chemokine production by senescent tumor cells supports NKG2Ddependent tumor elimination by natural killer cells. J. Exp.Med. 210, [10] Eggert, T. et al. (2016) Distinct functions of senescence-associated immune responses in liver tumor surveillance and tumor progression. Cancer Cell 30, [11] Wieland E et al. (2017) Endothelial Notch1 Activity Facilitates Metastasis. Cancer Cell, 31(3): [12] Agostini A et al. (2012) Targeted cargo delivery in senescent cells using capped mesoporous silica nanoparticles. Angew Chem Int Ed Engl, 51(42):

108 P-62 Sensing by upconversion nanohybrids based on analyte-induced drug delivery Estébanez B. Nestor L, 1 Andres Lorena, 1 Maria González-Béjar, 1,2 Julia Pérez-Prieto 1,2 Instituto de Ciencia Molecular(ICMoL)/ Departamento de Química Orgánica, Universidad de Valencia. C/ Catedrático José Beltrán 2, 46980, Paterna, Valencia (Spain) nesluis@uv.es Upconversion nanoparticles with an inorganic matrix doped with rare earths (e.g. NaYF 4 : Er 3+, Yb 3+,UCNP) have unique photophysical features, such as a large anti-stokes emission after near-infrared (NIR) excitation with a low-power continuous-wave diode laser. Due to the hydrophobic ligand (e.g. oleic acid) coordinating UCNPs after synthesis, further functionalization is necessary to provide them biocompatibility and dispersibility in water. 1 We have prepared functional water-dispersible UCNPs capped with a thin shell of a biocompatible copolymer HEMA-AMPS (2-hydroxyethylmethacrylate (HEMA) and 2- acrylamido-2-methyl-1-propanesulphonsulphonic acid (AMPS)) via sulfonate-multigrafting to the UCNP surface. The unusual stability of the UCNP organic capping to strong acid media makes the nanohybrid particularly suitable for many applications where the maintenance of the UCNP capping is crucial for their performance. 2 Some sulphonate groups of the copolymer (COP) would be involved in its binding to the UCNP surface, while others would be at the nanohybrid periphery and consequently, it is possible to decorate them with cationic dyes such as methylene blue (MB). The successful assembly of UCNP@COP and MB led to UCNP@COP@MB nanohybrids. Remarkably, MB progressively detached upon increasing [HCl]. The dependence of the R/G emission ratio of UCNP@COP@MB in the 2-7 ph range showed a good correlation with the ph (Figure 2). 2 This is an interesting example of sensing by an UCNP nanohybrid based on analyte-induced dye delivery. References [1] A. Gnach and A. Bednarkiewicz. Nano Today, 2012, 7, [2] I.Recalde, N. Estebanez, L. Francés-Soriano, M. Liras, M. González-Béjar, Julia Pérez-Prieto. Nanoscale, 2016, 8,

109 P-63 BACTERIAL RECOGNITION: MODELS COMPARISON, ACCURACY, RELIABILITY AND DIAGNOSTIC PLOTS María José Medina-Hernández 1, Laura Escuder-Gilabert 1, Yolanda Martín-Biosca 1, Mireia Pérez-Baeza 1, Salvador Sagrado 1,2 1 Departamento de Química Analítica, Universitat de València, Burjassot, Valencia, Spain. lescuder@uv.es 2 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM). Universitat Politècnica de València, Universitat de València. Avd. Vicente A. Estellés s/n, Burjassot, Valencia, Spain. Keywords: bacterial recognition, accuracy, modelling Among the biodegradation models proposed to perform parameter estimation from a substrate depletion curve, the Monod equation and its simplified versions, e.g. the Michaelis-Menten-like equation, are the most used. However, both models are problematic in terms of accuracy of kinetic parameters due to linear parameter correlation [1]. In this communication, simulated data in realistic conditions are used to highlight the pitfalls related to model the selection for estimation. Combined Scenarios are used (Scenario-[11] = Simulated data and estimations comes from Monod model; Scenario-[22] = Simulated data and estimations comes from Michaelis-Menten-like model, etc.). Their accuracy and coefficients reliability are evaluated by means of novel diagnostic plots, such as the Predictive (Pp) vs. Descriptive (Dp) power plot as a function of analyte concentration (S 0 ) and half saturation constant (Ks) (Fig. 1), among others Pp [11] 50 Pp [22] Dp [11] Dp [22] Fig.1. Pp-Dp plots including estimations for Scenario-[11] and Scenatio-[22]. Log (S 0 / K s ) levels: -3 ( ), -2 ( ), -1 ( ), 0 ( ) and 0.4 (+). 100 simulations per level. Acknowledgements This work has been supported by the Project CTQ R (MINECO/FEDER, UE). References [1] C. Liu, J.M. Zachara. Environ. Sci. Technol. 2001, 35,

110 P-64 PBI COMPOSITE MEMBRANES CONTAINING COBALTACARBORANE SALTS AS POLYMERIC ELECTROLYTES AT HIGH TEMPERATURE Jorge Escorihuela 1, Isabel Fuentes 2, Abel García-Bernabé 1 Clara Viñas 2, Francesc Teixidor 2 and Vicente Compañ 1 1 Departamento de Termodinámica Aplicada, Universitat Politècnica de València, Camino de Vera, s/n, Valencia (Spain). joresfu1@upvnet.upv.es 2 Institut de Ciència de Materials de Barcelona, ICMAB-CSIC, Campus Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain 3 keywords: polymeric electrolyte; metalcarborane; PBI Proton exchange membrane fuel cells (PEMFCs) are receiving considerable attention in the past decades as alternative and sustainable electrochemical energy conversion devices. PEMFCs use a proton conductive polymer membrane as electrolyte, which ideally should possess high proton conductivity, low cost low fuel permeability, and excellent stability at high temperature. In the quest of developing low cost PEMs with high conductivity at elevated temperatures, the use of polybenzimidazole (PBI) has emerged as an alternative to commercial Nafion membranes due to its lower methanol crossover as well as higher thermal and mechanical stability. Previous investigations showed an enhanced conductivity measured by impedance spectroscopy of sodium versus lithium salts [1]. Herein, we investigate the proton conductivity of a series of polymeric composite membranes based on polybenzimidazole (PBI) containing different metalcarborane salts with chemical structure M[Co(C 2 B 9 H 11 ) 2 ] ( M[COSANE], M =H +, Li + or Na + ) as fillers of mixed matrix membranes (MMMs) [2]. N N H PBI solution NH N n Metalcarborane solvent evaporation References [1] I. Fuentes, A. Andrio, F. Teixidor, C. Viñas, V. Compañ, Phys. Chem. Chem. Phys. 2017, 19, [2] I. Fuentes, A. Andrio, A. García-Bernabé, J. Escorihuela, C. Viñas, F. Teixidor, V. Compañ, Phys. Chem. Chem. Phys. 2018, 20, Financial support by Spanish Ministerio de Economía y Competitividad (project ENE/ R). 110

111 P-65 FAST AND EFFICIENT METAL FREE CLICK CHEMICAL FUNCTIONALIZATION OF POLYMER BRUSHES Jorge Escorihuela 1,2, Digvijay Gahtory 3, Rickdeb Sen 3 and Han Zuilhof 3 1 Departamento de Termodinámica Aplicada, Universitat Politècnica de València, Camino de Vera, s/n, Valencia (Spain). joresfu1@upvnet.upv.es 2 Departamento de Química Orgánica, Facultad de Química, Universidad de Valencia, C/ Dr. Moliner 50, Burjassot (Valencia), Spain. 3 Laboratory of Organic Chemistry, Wageningen University and Research, Stippeneng 4, 6708 WE, Wageningen (The Netherlands) 3 keywords: metal free click; SPOCQ; polymer brushes The discovery and application of novel metal-free click reaction strategies is a growing domain that has garnered significant interest amongst (bio-)organic and material chemists[1]. Most of these reactions involved a strained reactant species (alkyne or alkene) that accelerates the reaction significantly or a highly facile bond exchange. The advantages of such reactions include faster kinetics and good-to-excellent yields. The strain promoted oxidation controlled cyclooctyne 1,2 quinone cycloaddition (SPOCQ) shown in Figure 1, is an example of such a click strategy[2]. Herein, we report a novel metal-free click reaction between 1 methyl 3 substituted cyclopropenes and o quinones and we also demonstrate the quantitative nature of the reaction on surfaces and demonstrate its versatility using anti fouling polymer brush functionalization[3]. References [1] J. Escorihuela, A. T. M. Marcelis, H. Zuilhof, Adv. Mater. Interfaces 2015, 2, [2] J. Escorihuela, A. Das, J. Looijen, F. L. v. Delft, A. Aquino, H. Lischka, H. Zuilhof, J. Org. Chem. 2018, 83, [3] D. Gahtory, R. Sen, A. R. Kuzmyn, J. Escorihuela, H. Zuilhof, Angew. Chem. Int. Ed. 2018, doi.org/ /ange

112 P-66 Anion receptors using thiophene and selenophene units as chalcogen bonding binding sites Encarnación Navarro-García, Antonio Caballero*, M. D. Velasco y Pedro Molina* Departamento de Química Orgánica, Facultad de Química, Universidad de Murcia, Campus de Espinardo, Murcia (España), encarnacion.navarro2@um.es Anion recognition, chalcogen bond, molecular receptor The detection and quantification of anion species through the development of new anion chemosensors [1] is a relevant area of interest to the scientific community due to the important role that these species play in a large number of biological and medical systems. In addition, some anions cause environmental pollution. Molecular sensors are defined as molecules capable of selectively and reversibly binding to an species and are usually classified according to the type of interaction involved in the recognition process: electrostatic interactions, hydrogen bonds, anion-π interactions or halogen bonds. The increase of the computational capacity has allowed to the theoretical chemists to find new non-novalent interactions of high interest based in the existence of the denominated σ-hole. Recently, theoretical calculations obtained by some researchers show regions of positive charge density in the atoms of the oxigen group [2]. Motivated by these results, the purpose of this research is the synthesis and the anion binding study of new receptors 1 and 2 bearing the benzene unit as spacer and the thiophene or selenophene as chalcogen bonding binding sites. 1 H-NMR experiments performed on the receptors 1 and 2 demonstrate that the receptors behaves as a molecular receptor for the F - Cl, Br -, I, and NO 3. The recognition process between receptor 1-2 and the anions consists of chalcogen bonds between the S or Se of the thiophene or selenophene and the anions. We acknowledge to Ministerio de Economía y Competitividad of Spain and FEDER projects CTQ P and CTQ P, and Fundación Séneca Región de Murcia (CARM) projects (18948/JLI/13 and 19337/PI/14). Referencias [1] Evans, N. H.; Beer, P. D. Angew. Chemie - Int. Ed. 2014, 53, 2-41 [2] Politzer, P.; Murray, J. S.; Clark, T.; Resnati, G.; Phys. Chem. Chem. Phys. 2017, 19,

113 P-67 NEW BODIPY DYE FOR THE DETECTION OF β-galactosidase Borja Díaz de Greñu 1,2, María Alfonso 1,2, Andrea Bernardos 1,2,4, Ramón Martínez- Máñez 1,2,3,4 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM) Universitat de València-Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain. bordiade@upvnet.upv.es 2 CIBER de Bioingeniería Biomateriales y Nanomedicina (CIBER-BBN), Spain. 3 Unidad Mixta de Investigación en Nanomedicina y Sensores. Universitat Politècnica de València, Instituto de Investigación Sanitaria La Fe, Valencia, Spain 4 Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Valencia, Universitat Politècnica de València, Centro de Investigación Príncipe Felipe, Valencia Spain. Senescence, detection, bodipy β-galactosidase (β-gal) is the enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. β-gal is commonly used as a reporter to examine transcription and transfection efficiencies [1], and as a very important biomarker for primary ovarian cancers [2] and cell senescence [3]. In particular, our research group is interested in the development of sensors and prodrugs able to selectively detect and target senescent cells, because avoiding their accumulation in aged organisms has been associated with an enhanced healthspan [4]. Fluorogenic probes are excellent agents to image β-gal enzymatic activity non-invasively in living cells. Although many fluorogenic probes for its detection have been developed, many of them lack of suitable absorption and emission wavelengths for in vitro experiments, stability, water solubility, and low quantum yield [5]. Herein, we present the synthesis of a new boron dipyrromethene (bodipy) sensor that emits fluorescence only after enzymatic activation. Thanks to the optimized structure, the probe is highly fluorescent, water soluble and red emitting, allowing the detection of β-gal not only in solution but also in senescent cells. [1] E. Schenborn, D. Groskreutz, Mol. Biotechnol., 1999, 13, [2] D. Asanuma, M. Sakabe, M. Kamiya, K. Yamamoto, J. Hiratake, M. Ogawa, N. Kosaka, P.L. Choyke, T. Nagano, H. Kobayashi, Nat. Commun., 2015, 6, [3] G.P. Dimri, X. Lee, G. Basile, M. Acosta, G. Scott, C. Roskelley, E.E. Medrano, M. Linskens, I. Rubelj, O. Pereira-Smith, M. Peacocket, J. Campisi. Proc. Natl. Acad. Sci. USA., 1995, 92, [4] R. Yosef, N. Pilpel, R. Tokarsky-Amiel, A. Biran, Y. Ovadya, S. Cohen, E. Vadai, L. Dassa, E. Shahar, R. Condiotti, I. Ben-Porath, V. Krizhanovsky. Nat. Commun., 2016, 7, [5] See for example: B. Lozano-Torres, I. Galiana, M. Rovira, E. Garrido, S. Chaib, A. Bernardos, D. Muñoz-Espín, M. Serrano, R. Martínez-Máñez, F. Sancenón, JACS, 2017, 139, ; E.- J. Kim, R. Kumar, A. Sharma, B. Yoon, H.M. Kim, H. Lee, K.S. Hong, J.S. Kim, Biomaterials, 2017, 122, 83-90; L. Peng, M. Gao, X. Cai, R. Zhang, K. Li, G. Feng, A. Tong, B. Liu, J. Mater. Chem. B, 2015, 3, ; H.W. Lee, C.H. Heo, D. Sen, H.-O. Byun, I.H. Kwak, G. Yoon, H.M. Kim, Anal. Chem., 2014, 86, Acknowledgements: B. DdG. gratefully acknowledges for his Juan de la Cierva Formación contract to Ministerio de Economía, Industria y Competitividad from Spain Government. 113

114 P-68 Dextran hydrogel to immobilize biomolecules on microarray format Zeneida Díaz Betancor 1, María José Bañuls-Polo 1,2, Ángel Maquieira Catalá 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico IDM, 2 Departamento de Química, Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, Valencia 46022, España, zediabe@upv.es Keywords: Hydrogel, fluorescence, proteins, microarray Different hydrogels were reported in the literature to create generic platforms for protein microarray applications [1]. Here, a novel strategy was developed to obtain high performance microarrays. It uses a dextran hydrogel to covalently immobilize oligonucleotides and proteins. This method employs aqueous solutions of dextran methacrylate (Dx-MA), which is a biocompatible and photopolymerizable monomer [2]. The approach promotes by light, at the same time, the polymerization and the covalent attachment of the capture probes inside the hydrogel by a thiol-ene coupling reaction. The hydrogel microarrays were assayed on different surfaces, as Blu-ray disk, polycarbonate or alkene functionalized glass slides, showing high probe immobilization densities and biorecognition yields. This methodology presents the advantages of reduced cost and time of analysis, low limit of detection, and multiplex capability, among others [3, 4]. Confocal fluorescence microscopy analysis demonstrates that the receptor immobilization and the biorecognition event are taking place inside the hydrogel. CCD Confocal BD CCD v-a Figure 1. Spots of Dx-MA in different surfaces: BD and acrylate-functionalized glass. References [1] Zhou, Y., Andersson, O., Lindberg, P. & Liedberg, B. Microchim. Acta 2004, 147, [2] van Dijk-Wotthuis, W. N. E. et al. Macromolecules 1995, 28, (1995). [3] S. Morais, R. Puchades, Á. Maquieira. Analytical and Bioanalytical Chemistry, 2016, 48, [4] S. Morais, L. Tortajada, Á. Maquieira. Expert Review of Molecular Diagnostics, 2014, 14, Acknowledgment Financial support from FEDER and Spanish MINECO (CTQ R). Biosensing of mutations located in KRAS oncogene in colon cancer patients based on blocked amplification 114

115 P-69 The use of Metal-Organic Frameworks as Powerful Contrast Agents in Magnetic Resonance Imaging Alejandro Cabrera-García 1, Zeneida Díaz-Betancor 2, Vincent Blay 3 1 Instituto de Tecnología Química, Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas, Av. de los Naranjos s/n, Valencia, Spain. 3 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, Av. de los Naranjos s/n, Valencia, Spain. 3 The Ohio State University Fisher College of Business zediabe@upvnet.upv.es Keywords: MOFs, contrast agents, MRI The use of nanomaterials in illnesses detection and treatment is a recent research field in materials science. Among these materials, we remark metal-organic frameworks (MOFs). MOFs are built by self-assembly of metal cations or clusters as nodes and polydentate organic molecules as linkers. These materials have demonstrated being useful as vectors for the smart controlled release of bioactive molecules and for the transport of contrast agents that could improve diagnosis through magnetic resonance imaging (MRI). [1] MRI is a powerful clinical tool which allows obtaining anatomic images with great penetration depth in soft tissues. However, MRI is relatively insensitive and requires administration of a high amount of contrast agent to differentiate between normal and ill tissues. In this regard, MOFs constitute tunable and effective carriers for contrast agents offering benefits such as 1) a higher concentration of paramagnetic metal centers in the same device and 2) a higher relaxivity centered on the metallic nodes than the small molecules usually employed as contrast agents in clinical practice. [2] Furthermore, MOFs can be stabilized and functionalized to optimize their performance under biological conditions reducing toxic effects of soluble contrast agents currently used in MRI. [3] References [1] He, C.; Liu, D.; Lin. W. Polymers. Chem. Rev. 2015, 115, [2] Della Rocca, J.; Liu, D.; Lin, W. Acc. Chem. Res. 2011, 44, [3] Horcajada, P.; Gref, R.; Baati, T.; Allan, P.K.; Maurin, G.; Couvreur, P.; Férey, G.; Morris, R.E.; Serre, C. Chem. Rev. 2012, 112, Acknowledgment A. Cabrera-García thanks the La Caixa Foundation for his Ph.D. scholarship. V. Blay thanks the support from the Valencian Ministry of Education. 115

116 P-70 Capped Mesoporous Silica Nanoparticles for the Selective Detection of thiosulphate Cristina de la Torre 1,2,3, Maurizio Liccheli 3, Ramón Martínez-Mañez 1,2, Félix Sancenón 1,2 1 Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia (Spain). cridela2@upv.es 2 Instituto Interuniversitario de Investigacion de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universidad Politècnica de Valencia- Universidad de Valencia 3 Dipartimento di Chimica, Università di Pavia, via Taramelli 12, I Pavia (Italy) Key words: Hydrogen sulfide, sensing, mesoporous nanoparticles. Thiosulfate anion is a reducing species that can be found in industrial wastewater, in aquifers and in human urine. It is very common to analyze wastewater from mining operations. In addition, its detection and quantification in hydrothermal waters is important due to the geochemical processes in which sulfur is involved. On the other hand, it is a bioindicator of exposure to hydrogen sulfide, the deficiencies of the enzyme sulfite oxidase and Down syndrome [1]. Normally, ionic chromatography or capillary electrophoresis is used for their separation and then detected by conductivity measurements. Taking into account the total absence of molecular-based chromophogenetic sensors or the use of solids, we propose the synthesis of an organicinorganic hybrid material for sensitive and selective detection of the thiosulfate anion. The silica nanoparticles will be used as an inorganic support whose pores will be loaded with various colorants / fluorophores. The outer surface of the charged nanoparticles will be functionalized with a Cu (II) complex and then the pores will be blocked by the addition of a bulky anion (hexametaphosphate, ATP) by electrostatic interactions with the cation. The idea is that in the presence of the thiosulfate anion (which has a high affinity for Cu (II) the demetallization of the anchored complex occurs, the separation of the bulky anion and the release of the dye [2]. The selectivity of the sensor material in the presence of other anions will be studied. The thiosulfate detection limit and the possible application of the nanoparticles in the analysis of real samples will also be determined. Referencias [1] I. Mikami, E. Shibayama, M. Yuzawa, Y. Miura, Anal.Sci. 2012, 28, [2] S.El Sayed, M.Liccheli, R.Martínez-Mañez, F.Sancenón, Chem.Asian J. 2017, 12,

117 P-71 SENSIBILIDAD AL DISOLVENTE DE NANOPARTÍCULAS DE ORO PASIVADAS CON NAD Daniel Cuaran 1, Elena Zaballos-García 1, Julia Pérez-Prieto 2 1. Departmento de Química Orgánica. Universidad de Valencia, Av. Vicent Andres Estelles s/n, 46100, Burjassot, España, dannc91@gmail.com 2. Instituto de Ciencia Molecular (ICMol), Universidad de Valencia, Catedrático José Beltrán 2, 46980, Paterna, Valencia, España Palabras clave: nanopartículas, fotoluminiscencia, disolventes. Las nanopartículas de oro con diámetros >2-100 nm se conocen como NP plasmónicas (AuNP) y forman coloides muy coloreados que han sido utilizados como sensores colorimétricos. [1,2] Sin embargo, las AuNPs son escasamente fluorescentes. Por el contrario, los nanoclusters de oro (AuNC) presentan diámetros 2 nm, pueden formar coloides incoloros y, en general, presentan fotoluminiscencia. [2-4] Las excelentes propiedades ópticas de estos nanomateriales combinado con su baja toxicidad motiva su aplicación como biosensores [5], así como en catálisis [6]. Siguiendo con nuestra línea de investigación y aplicación de AuNPs y AuNCs, en este trabajo presentamos la síntesis de coloides acuosos de AuNPs de 10.3 nm ± 3.0 nm, pasivados con el dinucleótido de adenina y nicotinamida (NAD), y estudio de su respuesta a la adición de disolventes de diferente naturaleza (50% v/v). El nanohíbrido AuNP@NAD se ha obtenido a partir de HAuCl 4.3H 2 O en presencia de la sal sódica del ácido 4-(2-hidroxietil)piperazin-1-etanosulfónico (HEPES-Na) y NAD. La banda plasmónica se encuentra a 525 nm y las respuestas del coloide al disolvente son: i) poca sensibilidad del plasmon (éter dietílico, formamida, metanol, etanol, acetato de etilo), ii) desplazamiento batocrómico (trietilenglicol, TEG) o aumento del scattering (DMSO) y iii) formación de agregados y su precipitación (THF, 1,4-dioxano, acetona); cabe destacar que estos agregados se redisuelven en agua dando lugar a coloides rojizos (acetona, Fig. 1b) o agregados dispersables en agua (THF, 1,4-dioxano (Fig. 1c); por otra parte, los sobrenadantes presentan luminiscencia, indicando la presencia de AuNC (Fig. 1b, 1c). Absorbancia 1.5 AuNP@NAD in TEG 1.0 a) AuNP@NAD in DMSO b) AuNP@NAD precipitadas en c) AuNP@NAD "rojas iniciales" acetona y redispersas en H2O AuNC@NAD en acetona (sobrenadante) Absorbancia Longitud de Onda [nm] Longitud de Onda [nm] Esquema 1. Comportamiento del coloide acuoso de AuNP@NAD tras la adición de disolventes Longitud de Onda diferente [nm] naturaleza (50% v/v): a) AuNP@NAD iniciales en H 2O, DMSO y TEG; AuNP@NAD agregadas en acetona (b) o 1,4-dioxano (c) y redispersados en H 2O; b,c) Inset: Fluorescencia del sobrenadante bajo luz UV. Referencias [1] Y.K. Zheng, L.M. Lai, W.W. Liu, H. Jiang, X.M. Wang, Adv. Coll. Interface Sci. 2017, 242, [2] J.P. Vanegas, L.E. Peisino, S. Pocovi-Martinez, R.J. Zaragoza, E. Zaballos-García, J. Pérez-Prieto. Chem. Eur. J. 2013, 19, [3] J. P. Vanegas, E. Zaballos-García, M. González-Béjar, P. Londoño-Larrea, J. Pérez-Prieto. RSC Adv., 2016, 6, [4] P. Londoño-Larrea, J.P. Vanegas, D. Cuaran, E. Zaballos-García, J. Pérez-Prieto. Chem. Eur. J. 2017, 23, [5] M.A.H. Muhammed, P.K. Verma, S.K. Pal, A. Retnakumari, M. Koyakutty, S. Nair, T. Pradeep. Chem. Eur. J. 2010, 16, [6] Y. Zhou, Z.F Ma. Sens. Actuators B. 2017, 241, Absorbancia AuNP@NAD precipitadas en 1,4-dioxano y redispersas en H2O AuNC@NAD en 1,4-dioxano (sobrenadante)

118 P-72 SISTEMA DE AYUDA PARA EL ATERRIZAJE AUTÓNOMO DE CUADRICÓPTEROS BASADO EN SENSORES LÁSER Claudia Lozano, Rafael Masot, Miguel Alcañiz Departamento de Ingeniería Electrónica, Universidad Politécnica de Valencia, Palabras clave: sensor láser, VL53L0X, Arduino La aparición de vehículos aéreos no tripulados o UAVs, ha desafiado al diseño de nuevos sistemas de sensores electrónicos capaces de realizar mediciones fiables, precisas y computacionalmente efectivas. El conocimiento exacto de la altura y la evitación de obstáculos en tiempo real es uno de los aspectos cruciales cuando de navegación autónoma de UAVs se habla. Su éxito depende, en gran medida, de la calidad y robustez de los sensores utilizados para adquirir datos del entorno y de su implementación sobre el sistema de control del vehículo. Sin embargo, muchas de las tecnologías utilizadas comúnmente para la medición de alturas y evitación de obstáculos presentan deficiencias, sobre todo en entornos desconocidos o indoor. En consecuencia, se presenta una prueba de concepto consistente en la estimación de alturas y reconocimiento de obstáculos mediante el uso de un dispositivo de bajo coste capaz de medir distancias de forma precisa: el sensor láser VL53L0X diseñado por ST Microelectronics. La plataforma de pruebas consiste en la estructura de un cuadricóptero equipado con cuatro sensores láser VL53L0X situados en el extremo de cada uno de sus brazos y un módulo de ultrasonidos HC-SR04 localizado en la parte central. La aplicación trabaja en un plano horizontal, y tanto en entornos de exterior, como de interior. El enfoque se fija en la correcta y precisa estimación de la altura mediante la lectura de los cinco sensores para detectar la existencia de zonas seguras de aterrizaje (sin obstáculos en su interior). Teniendo en cuenta las características de ambos tipos de sensores, los ensayos experimentales de caracterización y validación demuestran su viabilidad de los sensores empleados, así como su eficaz aplicación como sistema de ayuda al aterrizaje autónomo de un cuadricóptero, integrado como parte de su sistema de control. Figura 1. Sensor VL53L0X, diagrama de bloques y prototipo final. Referencias [1] Umberto Papa, Giuseppe Del Core. IEEE Metrology for Aerospace. 2015, [2] John Dougherty, Daewon Lee, Taeyoung Lee. American Control Conference. 2014,

119 P-73 Silanized glass as hybridization platform for mirna detection Juan Chorro-Grau 1, Miguel A. González-Martinez 1,2, Ángel Maquieira 1,2, Luis A. Tortajada-Genaro 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM). Universitat Politècnica de València-Universitat de València. Camino de vera s/n, Valencia. 2 Depart. Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain otitxu1013@gmail.com Microarray, Biosensing, mirna The last discoveries about characteristic microrna (mirnas) profile expression for several kind of diseases, cancers specially, have shown an unlimited potential of these molecules as biomarkers for diagnosis in clinical ambit. Their monitoring in accessible body fluids may gave us information about the type, stage and possible response to drugs in hidden oncogenic process [1]. This study presents the development of a rapid and affordable method based on hybridization in microarray format on silanized glass for the detection of circulating micrornas (mi-rnas) related to oncological processes. Glass surfaces have been silanized with several vinyl organosilanes (C3-C22) and spacing probe densities. Two strategies of probe immobilization have been studied. First, 5 -end thiolated probe was covalently anchored to the vinyl group using thiol-ene photochemical linking. The second strategy was based on thiolated biotin covalently anchored and streptavidin-biotin binding using 5 -end biotinylated probes [2]. Both methods have been applied to solid phase hybridization assays with synthetic mirnas (mir-21, mir-151 and mir-191). Hybrids were displayed colorimetrically and quantified by image technique analysis (flatbed scanner). The resulting device was able to provide selective, sensitive and multiplex quantification of target analytes, being useful in the first steps of diagnosis of poorly differentiated tumours. Financial support from the GVA (PROMETEO/2014/40), FEDER and the Spanish MINECO (CTQ R) is acknowledged. References [1] Per Hydbring, Gayane Badalian-Very. Clinical applications of micrornas, F1000 Res. 2013, 2, 136. [2] Jorge Escorihuela, María-José Bañuls, Santiago Grijalvo, Ramón Eritja, Rosa Puchades, Ángel Maquieira, Direct Covalent Attachment of DNA Microarrays by Rapid Thiol Ene Click Chemistry. Bioconjugate Chem. 2014, 25,

120 P-74 DISEÑO DE UN QUIMIODOSÍMETRO COLORIMÉTRICO PARA LA DETECCIÓN DE NO 2 Y SO 2 Samuel A. Ceballos 1,2, Andrés Sala 1,2, Salvador Gil 1,2, Ana M. Costero*,1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València; Doctor Moliner 50, 46100, Burjassot, Valencia, España., Samuel.Ceballos@uv.es 2 Departamento de Química Orgánica, Universitat de València, Doctor Moliner 50, 46100, Burjassot, Valencia, España. 3 palabras clave: Sensor, NO 2, SO 2 El NO 2 y el SO 2 son dos de los principales gases que provocan la contaminación atmosférica y la lluvia ácida. Ambos son tóxicos para los seres humanos, por lo que su detección es de vital importancia, sobretodo en lugares de trabajo dónde puedan generarse. Para ello, hemos utilizado el compuesto tetrakis(4-dimetilaminofenil)etileno (TPEDMA), que es capaz de oxidarse mediante la pérdida de dos electrones para dar una especie dicatiónica de intenso color morado 1. El NO 2 (g) produce una oxidación rápida del TPEDMA en disolución en presencia de agua, mientras que el SO 2 (g) no es capaz de producir cambios, como es de esperar de un reductor. Sin embargo, si añadimos hidroperóxido de terc-butilo a la disolución, el SO 2 (g) es capaz de provocar la oxidación del TPEDMA por medio de radicales intermedios, permitiendo así su detección. Figura 1: Reacciones de oxidación del TPEDMA utilizadas. Referencias [1] S. Hünig, M. Kemmer, H. Wenner, F. Barbosa, G. Gescheidt, I. F. Perepichka, P. Bäuerle, A. Emge, K. Peters, Chem. - A Eur. J. 2000, 6, [2] B. D. Flockhart, K. J. Irvin, R. C. Pink, B. D. Sharma, Chem. Commun. 1971,

121 P-75 ENSAYO IN VITRO PARA LA DETECCIÓN MULTIPLEXADA DE ALERGIAS ALIMENTARIAS Natalia Casañ-Raga 1, Salvador Más 1, Ángel Maquieira 1,2, Luis A. Tortajada-Genaro 1,2, Dolores Hernández Fernández de Rojas 3, Ethel Ibáñez-Echevarría 3, Sergi Morais 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM). Universitat Politècnica de València-Universitat de València. Camino de Vera s/n, Valencia. 2 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia. 3 Hospital Universitari i Politènic La Fe, Servicio de Alergología, Avinguda de Fernando Abril Martorell, 106, València, Spain nacara@etsiamn.upv.es 3 Palabras clave: Alergia, alimentos, inmunoglobulina E Los alérgenos alimentarios pueden provocar reacciones adversas de hipersensibilidad de tipo I. Este proceso presenta una elevada prevalencia que puede afectar a la población sensibilizada vía contacto, ingestión o inhalación del alérgeno. En la actualidad, esta enfermedad afecta al 4% de la población (6% de los niños), lo que representa un impacto significativo en la calidad de vida y en los costes sanitarios asociados [1]. La creciente demanda de información de salud sobre los problemas alérgicos genera la necesidad de disponer de mejores métodos de detección no invasivos y sensibles que proporcionen resultados fiables de manera rápida y sencilla [2]. Se desarrolla un ensayo multiplexado que permita la determinación simultánea en suero sanguíneo de inmunoglobulinas E específicas para varios alimentos alergénicos en un solo experimento. La aproximación consiste en la inmovilización de un panel de 12 alérgenos alimentarios (recogidos en la normativa de la UE), en formato de micromatriz. Tras la incubación de la muestra (25 µl) y el revelado, se procede a la lectura de los resultados mediante un lector de discos digitales ópticos en modo reflexión. El procesado de las imágenes proporciona señales relacionadas con la concentración de inmunoglobulina E específica para cada uno de los alérgenos. Esta metodología tiene buenas prestaciones, en lo que se refiere a sensibilidad y selectividad, emplea bajo volumen de muestra y un consumo reducido de reactivos auxiliares, es rápida (60 min) y fiable. La información obtenida puede dar una idea directa del nivel de sensibilización de los individuos a los alimentos estudiados. AGRADECIMIENTOS: FEDER, GVA PROMETEO II /2014/040 y MINECO CTQ R. [1] IGLESIAS, E.M. Alergia a los alimentos. Pediatría Integral, 2018, p. 87. [2] Food Allergy. EAACI White Book 2018, p

122 P-76 WETTABILITY TUNING OF GLASS FIBERS BY SILANIZATION AND THE EFFECT ON BIOMOLECULAR RECOGNITION EVENTS Carlos De-Barutell 1, Miguel Á. González-Martínez 1,2, Pilar Aragón 1,2, Patricia Noguera 1,2, Ángel Maquieira 1,2 (1) Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Camino de Vera s/n, E46022 Valencia, Spain (2) Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain Palabras clave: hydrophobicity, biotin-strpetavidin interaction The use of glass fibers as a solid support for biomolecular recognition reactions, especially protein-protein interactions, is proposed. As a proof of principle the interaction biotin-streptavidin has been chosen. For this, wettability of fiber surfaces was modified either using individual alkenyl silanes (with different chain length) or the same alkenyl silanes mixed with a fluoroalkenyl silane. Arrays of a thiol-biotin derivative were anchored by means of the photochemical click thiol-ene reaction on the surfaces. Finally, streptavidin-cy5 was allowed to react with the anchored biotin and the image of the fluorescence spots attained was appropriately treated to achieve intensity, size and SNR values. Results show how hydrophobicity affects signals achieved on the different surfaces, improving the quality of the spots as the surfaces becomes more hydrophobic, due to the use of a longer chain silane (larger water contact angle -WCA-, Figure 1) or to the addition of the fluoroalkenyl silane (Figure 2). When comparing different silanes, significant differences were achieved for water contact angle, SNR and intensity values, showing the feasibility of this system in biomolecular recognition events. WCA: 0º º WCA: 0º C2 C11 WCA: 130º Figure 1. Signal and WCA of two silanes: C2 (short) and C11 (long) C4 C4+2 WCA: 130º Figure 2. Signal and WCA of C4 with the adition of a fluoroalkenyl silane (2 %) Financial support from the GVA (PROMETEO/2014/40), FEDER and the Spanish MINECO (CTQ R) is acknowledged. 122

123 P-77 A new design to treat glioblastoma multiforme using loaded prodrug and the specific expression of a cleavage introduced gen Vicente Candela-Noguera 1,2,3, Gema Vivo Llorca 1,2,3, Borja Díaz de Greñu 1,2, Elena Aznar 2,1,3, María Dolores Marcos 1,2,3,4, Ramón Martínez-Máñez 1,2,3,4 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022, Valencia, Spain. 2 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain. 3 Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina. Universitat Politècnica de València, Centro de Investigación Príncipe Felipe, Valencia, Spain 4 Departamento de Química, Universitat Politècnica de València, Camí de Vera s/n, 46022, Valencia (Spain). 3 keywords: glioblastoma multiforme, prodrug, genetic therapy Glioblastoma multiforme (GBM) is one of the most aggressive and common nervous central system primary brain tumour, having a short expectance nearby to 15 months. In addition, the treatments used up to now are ineffective, due to the drugs difficulty to cross the blood-brain barrier and the aggressiveness of surgery intervention followed by radiation or chemotherapy [1]. The aim of this project is to prepare mesoporous silica nanoparticles with control release features and useful as a drug carrier toward the brain. The project will develop two types of nanoparticles: (i) nanoparticles loaded with doxorubicin derivatised with galactose (DOXO-Gal), as a pro-drug without toxicity, and capped with polyethylenegycol (PEG) attached through a disulphide bond; (ii) nanoparticles which carries a plasmid bound with polyethyleneimine (PEI). The plasmid is coding for beta-galactosidase (lacz) and is directed by a promoter induced by SOX11 [2], an overexpressed transcription factor specific of glioblastoma cell lines [3]. In a first step glioblastoma cells were treated with the second nanoparticles in order to induce the expression of beta-galactosidase. Then, in a second step, the cells are treated with the first type of nanoparticles which are internalized. The internal reducing cell environment induced the release of the DOXO-Gal which is hydrolyzed to DOX in the presence of beta-galactosidase. Thus, beta-galactosidase is specifically expressed on tumour cells and it cleavages the prodrug released by the first nanoparticle inside these, yielding the selective cytotoxic effect. Besides, both nanoparticles will be functionalized with targeting molecules (such as antibodies, receptors or peptides). Our approach will be used for the development of smart innovative strategies to treat glioblastoma multiforme. References [1] C. Adamson, O.O. Kanu. AI. Mehta, C. Di, N. Lin, A.K. Mattox, DD. Bigner, Expert. Opin Investig. Drugs 2009, 18, [2] K.M. Salerno, X. Jing, C.M. Diges, B.M. Davis, a K.M. Albers. Neuroscience 2013, 231: [3] B. Weigle, R. Ebner, A. Temme, S. Schwind, M. Schmitz, A. Kiessling, M.A. Rieger, G. Schackert, H.K. Schackert, E.P. Rieber. Oncology Reports 2005, 13:

124 P-78 In vivo synthesis of a senolytic drug using a biorthogonal reaction catalyzed with heterogeneous copper nanoparticles Juan F. Blandez 1,2, Beatriz Lozano-Torres 1,2,3, Irene Galiana 1,2,3, Félix Sanzenón 1,2,3,4 and Ramón Martínez-Máñez 1,2,3,4 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Camí de Vera s/n, Valencia. Spain. bealotor@upvnet.upv.es 2 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). 3 Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Universitat Politècnica de València, Centro de Investigación Príncipe Felipe, Valencia, Spain. 4 Unidad Mixta de Investigación en Nanomedicina y Sensores. Universitat Politècnica de València, IIS La Fe, Valencia, Spain Keywords: Biorthogonal reactions, Senescent cells, drug synthesis Biorthogonal reactions have been developed as a powerful approach for the study of biological processes in their native environment [1]. The main characteristics of these reactions are high selectivity and efficiency, and functionality in the complex biological environment of a cell [2]. The prototype of this group of reactions is the click reaction catalyzed by Cu(I). This reaction generated a triazole heterocycle using an alkyne and an azide as reagents [3]. Nowadays, this process is used in applications such as the synthesis of polymers and the fabrication of bioconjugated compounds. However, the in vivo use of this reaction is limited due to the toxicity of free Cu(I) [4]. For the above mentioned reasons, we propose here the use of a heterogeneous catalyst which consist in a mesoporous silica matrix (MCM-41, which act as support) where the copper nanoparticles (Cu-NPs) are anchored in order to avoid the toxicity of free metal. With this catalyst, we focus in the in situ synthesis of Combrestastatin (CA-4), this compound shows cytotoxic activity when the molecules are in the Z (or cis) conformation. However, when the drug acquired the E (or trans) conformation losses its toxicity [5]. The underlying idea of our nanoparticles is the production of the drug in situ using non-toxic precursors. To control the production of CA-4, galactan will be anchored onto the external surface of the nanoparticles. This will allow that Cu-NPS only will be active when the gate will be removed. Galactan gates are removed in the presence of enzyme β-galactosidase, enzyme which is overexpressed in senescence cells [6]. With this protocol is possible to control the in situ and in vivo generation of Combrestastatin drug under biological conditions and carried out the selective elimination of senescence cells using initial innocuous reagent compounds. References [1] J. A. Prescher, C.R. Bertozzi. Nat. Chem. Biol. 2005, 1, [2] H. W. Shih, D. N. Kamber, J. A. Prescher, Curr. Opin. Chem. Biol. 2014, 21, [3] V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless. Angew. Chem. Int. Ed. 2002, 41, [4] L. M. Gaetke, C. K. Chow. Toxicology, 2003, 189, [5] N O Boyle, M. Carr, L. M. Greene, O. Bergin, S. M. Nathwani. J. Med. Chem. 2010, 53, [6] B. Y Lee, J. A. Han, J. S. Im, A. Morrone, K. Johung, E. C. Goodwin, W. J. Kleijer, D. Dimaio, E. S. Hwang. Aging Cell, 2006, 5,

125 P-79 BIOTRANSPORTING NANOFACTORIES FOR ON-COMMAND PRODRUG RELEASE AND ACTIVATION Beatriz de Luis 1,2, Antoni Llopis-Lorente 1,2, Félix Sancenón, 1,2,3, Ramón Martínez- Máñez 1,2,3 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM). Universitat Politècnica de València - Universitat de València. Camí de Vera s/n, 46022, Valencia (Spain). beadelui@upvnet.upv.es 2 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain. 3 Departamento de Química, Universitat Politècnica de València, Camí de Vera s/n, 46022, Valencia (Spain). Keywords: Nanofactories, prodrug, Janus nanoparticles The principles of Enzyme Prodrug Therapy (EPT) have inspired the development of a vast array of novel advanced therapy devices [1]. Nanoreactors consist of confined spaces with enzymes inside where prodrugs in solution are activated via enzymatic reactions [2]. However, their performance is limited due to the low permeability of the prodrugs, the different tissue biodistribution of both nanoreactors and prodrugs as well as the limited nanoreactors synthesis strategies. In this work, we present a nanovehicle based on Janus Au-mesoporous silica nanoparticles loaded with a prodrug model (4-methylumbelliferyl-β-D-galactopyranoside) which apart from accomplishing the functions of a nanoreactor, it is capable of communicating with its surroundings. When a specific stimulus is received, a complex cascade of enzyme-mediated reactions is triggered leading to the prodrug release and activation. Therefore, these biotransporting nanofactories respond to the organism intrinsic biochemistry and can be considered a novel approach to the self-medication paradigm. The tremendous variety offered by hybrid nanomaterials regarding the nature of cargos, functionalization and targeting by means of well-known chemistry methodologies[3] let envisage a large number of nanodevices based on the described scaffold with potential applications in the field of biomedicine, catalysis, programmed synthesis and chemical communication. References [1] C. Luo, J. Sun, B. Sun, Z. He, Trends Pharmacol Sci. 2014, 35, [2] M. Godoy-Gallardo, M. J. York-Duran, L. Hosta-Rigau, Adv. Healthcare Mater. 2018, 7, [3] E. Aznar, M. Oroval, L. Pascual, J. R. Murguía, R. Martínez-Máñez, F. Sancenón, Chem. Rev. 2016, 116,

126 P-80 Light induced microarraying of half antibodies to detect cardiac biomarkers María-José Bañuls 1,2, Rafael Alonso 1, Rosa Puchades 1,2, Ángel Maquieira 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Camino de Vera s/n, E46022 Valencia, Spain, mbpolo@upvnet.upv.es 2 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain Thiol-ene coupling, antibody microarray, cardiac biomarkers The thiol-ene coupling reaction (TEC) fulfils all the desirable requirements of a click reaction[1]. TEC is normally initiated by UV light, which induces the formation of a thiol radical that reacts with a carbon-carbon double bond and leads to a thioether. This procedure is compatible with aqueous media chemistry, which makes possible its use with biomolecules. Although it has been successfully employed as a bioconjugation strategy with nucleic acids, aptamers and enzymes [2], its use with antibodies is restricted due to their lack of free thiols available to react. Here, we propose an approach based on the selective reduction of antibodies in their hinge region, and their covalent attachment onto an alkene functionalized surface through TEC photoreaction. The performance of these half-antibody microarrays generated by TEC dramatically improved the response compared to whole antibody microarrays. The proposed approach allowed us to successfully determine interesting analytes as the cardiac biomarkers C-reactive protein (CRP), cardiac Troponin I (ctni), and Myoglobin (Mb)[3]. The approach is applicable to a wide range of materials that can be functionalized with organosilane chemistry, and can selectively pattern antibodies on the surface by using selective irradiation through a photomask. S S S S TCEP HS HS HS HS hν HS S HS S Acknowledgement: This work was financed from the Horizon 2020 European Union programme (H PHOCNOSIS), MINECO project CTQ/2016/75749-R, FEDER and GVA PROMETEO II 2014/40. References [1] H. C. Kolb, M. G. Finn, K. B. Sharpless. Angew. Chem. Int. Ed. 2001, 40, [2] Y. Liu, W. Hou, H. Sun, C. Cui, L. Zhang, Y. Jiang, Y. Wu, Y. Wang, J. Li, B.S. Sumerlin, Q. Liu, W. Tan, Chem. Sci. 2017, 8, [3] R. Alonso, P. Jiménez-Meneses, J. García-Rupérez, M.-J. Bañuls, A. Maquieira. Chem Commun. 2018, accepted. 126

127 P-81 BOOSTED SENSITIVITY FOR CASEIN IMMUNOSENSING BY FOURIER DOMAIN ANALYSIS Miquel Avella-Oliver 1, Gabriel Sancho-Fornes 1, Javier Carrascosa 1, Rosa Puchades 1,2, Angel Maquieira 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, Valencia, Spain miavol@upvnet.upv.es 2 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia, Spain 3 keywords: Fourier domain analysis, sensitivity, casein Fourier domain analysis (FDA) is a novel approach to easily increase the sensitivity of heterogeneous biorecognition assays on solid substrates. It is based on patterning the probes as a set of periodic straight strips, applying the Fourier transform to the resulting data, and then using the frequency-domain peak as analytical signal. This strategy allows to discriminate the signal generated by the binding event of interest from every noise contribution, thus increasing signal-to-noise ratios and enabling more sensitive bioanalytical measurements (Fig. 1). In this communication, we report the application of FDA to a sandwich immunoassay to quantify casein. The immunochemical system relies on patterned capture monoclonal IgGs on the assay surface, incubating buffered solutions of casein, and then applying HRP-labelled primary IgGs followed by TMB precipitation. In addition to explore the potential of FDA in different bioreagents and in more complex assay schemes, this study also aims to provide insights into the applicability of this approach to quantify food allergens [1]. The results show that FDA improves signal-to-noise ratios about two orders of magnitude compared to standard microarray format, from 47 to 303 in the steady state of the corresponding dose-response curves. This increase provides a relevant sensitivity enhancement for this immunochemical system, which reaches a detection limit of 4 ng/ml (174 pm) and a quantification limit of 27 ng/ml (1.2 nm) for casein. This work was supported by MINECO (CTQ R), FEDER, and GVA (PROMETEO II/2014/040). [SNR] FDA µarray [casein] Fig. 1. Illustration of the sensitivity enhancement provided by FDA References [1] Badran A. A., Morais S., Maquieira A. Anal. Bioanal. Chem. 2017, 409,

128 P-82 ASSESSING DIFFERENT IMMOBILIZATION CHEMISTRIES TO CREATE BIOMOLECULAR GRATINGS BY MICROCONTACT PRINTING Augusto Juste-Dolz 1, Miquel Avella-Oliver 1, Estrella Fernandez 1, Rosa Puchades 1,2, Ángel Maquieira 1, 2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Camino de Vera s/n, E46022 Valencia, Spain aujusdol@upv.es 2 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain 3 keywords: microcontact printing, diffraction-based biosensing, label-free Biogratings are nanostructured networks of biomacromolecules patterned on solid substrates as diffractive gratings [1]. According to the concept behind diffraction-based sensing, biogratings allow detecting molecular-scale events by exploiting light diffraction modulations when interactions among targets and patterned biomolecules take place. In addition to enable real-time analysis of unlabelled biorecognition assays, these structures present a high potential to solve the problems associated with non-specific binding in complex matrixes [2]. Microcontact printing (µcp) is a practical method to create functional biogratings. It is based on a regioselective transfer of biomolecules from nanostructured elastomer stamps to flat solid substrates. The immobilization of probes in biogratings fabricated by µcp is typically mediated by passive physisorption. However, more stable attachment chemistries can enhance the performance of biogratings in some cases [3], and the convergence of µcp and covalent immobilization of biological probes remains rather unexplored. Herein we present an assessment of different chemistries to create biogratings by µcp on glass. In this study, passive physisorption and different covalent approaches are optimized and their performance in terms of probe transfer, functionality, and structuration is experimentally compared. For that, an immunoassay based on BSA as probes and IgGs as targets has been selected as a model system, and the biograting features have been evaluated by diffraction-based sensing, fluorescence scanning, contact angles, and atomic force microscopy. From a general viewport, this study aims to provide insights into the distinctive advantages of covalent biogratings to achieve the highest bioanalytical capabilities of diffraction-based sensing. This work was supported by the spanish MINECO (CTQ R), FEDER, GVA (PROMETEO II/2014/040) and PhD grant (UPV FPI 2017). References [1] Avella-Oliver M., Carrascosa J., Puchades R. Maquieira A., Anal. Chem., 2017, 89, [2] Gatterdam V., Frutiger A., Stengele K.-P., Heindl D., Lübbers T., Vörös J., Fattinger C., Nat. Nanotech., 2017, 12, [3] Wendeln C., Ravoo B.J. Langmuir, 2012, 28,

129 P-83 METALLIC NANOSTRUCTURES OF METALLICA Augusto Juste-Dolz 1, Miquel Avella-Oliver 1, Gabriel Sancho-Fornes 1, Rosa Puchades 1,2, Ángel Maquieira 1, 2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Camino de Vera s/n, E46022 Valencia, Spain aujusdol@upv.es 2 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain 3 keywords: silver, nanostructure, diffraction-based sensing The nanostructuration of inorganic surfaces is one of the basis of nanoscience and nanotechnology, and plays a key role in the scientific progress in many areas [1]. For biosensing, biomacromolecules are typically immobilized on nanostructured materials in order to exploit nanoscale light-matter phenomena to transduce biointeractions. However, the advances in this direction are hindered by the complexity, expensiveness, and low scalability of the standard nanofabrication techniques in the current state-of-the-art [2]. In this work, we present a novel and versatile approach to create nanostructured silver surfaces. It relies on patterning structured networks of bioreceptors on solid substrates by microcontact printing [3], performing a biorecognition assay comprising labelling with gold nanoparticles, and finally incubating Ag + solutions on them. Thus, gold nanoparticles act as nucleation centres and nanostructured reliefs of metallic silver are developed on the surface. Herein we report the introduction, optimization, and characterization of this approach, and demonstrate its capabilities to fabricate reproducible and large-scale silver nanostructures of controllable thicknesses on different materials (glass, polycarbonate, and silicon). Fig. 3. Silver nanostructure of the binary data of a Metallica song encoded in a CD-ROM. This work was supported by MINECO (CTQ R), FEDER, GVA (PROMETEO II/2014/040) and PhD grant (UPV FPI 2017). References [1] Ravoo B.J. J. Mater. Chem. 2009, 19, [2] Xing J. J. Phys. D: Appl. Phys. 2016, 49, 29LT01. [3] Avella-Oliver M., Carrascosa J., Puchades R. Maquieira A., Anal. Chem., 2017, 89,

130 P-84 A CELLULAR MODEL TO EVALUATE THE ELIMINATION OF CARDIAC SENESCENT CELLS BY TREATMENT WITH SENOLYTIC NANOPARTICLES Araceli Lérida Viso 1,2, Pilar Sepúlveda 3, Mar Orzáez 4,5, Ramón Martínez-Máñez 1,2,4,6 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València. Valencia, Spain. arlevi@doctor.upv.es 2 Unidad Mixta UPV-La Fe de Nanomedicina and Sensores, IIS La Fe. Valencia, Spain 3 Unidad de Regeneración y Transplante Cardíaco, IIS La Fe. Valencia, Spain 4 Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina. Valencia, Spain 5 Centro de Investigación Príncipe Felipe. Valencia, Spain 6 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Valencia, Spain Keywords: Cardiotoxicity, senescence, nanoparticles Senescence is a process by which damaged cells enter a non-reversible cell cycle arrest. However, these cells maintain an active metabolism and a highly senescence-associated secretory phenotype (SASP) that alters the surrounding microenvironment [1]. Many antineoplastic agents used in clinic induce premature senescence in healthy tissues generating accelerated aging processes and adverse side-effects in patients [2]. Cardiotoxicity is a well-known limiting factor of anticancer treatment with doxorubicin (DOXO), the most effective anthracycline, that leads to long-term morbidity and mortality. DOXO exposure severely affects the population of cardiac progenitor cells in human hearts (hcpcs) by inducing premature senescence and may represent the cellular basis of DOXOinduced cardiomyopathy in humans [3]. The main goal of this project is to achieve the specific elimination of these senescent cells to avoid non-desired side-effects by using a nanosystem based on gated mesoporous silica nanoparticles, previously reported in our group. This nanosystem selectively releases a senolytic cargo in senescent cells [4]. In this work we have studied the induction of senescence in rat neonatal cardiomyocytes by two chemotherapeutic agents, DOXO and palbociclib, commonly used in clinical practice. Our results demonstrate the ability of these drugs to induce a senescent phenotype in cardiac cells. This phenotype is characterized by increased senescence-associated-βgalactosidase (SA-β-gal) activity and expression of senescence markers, as the cell cycle inhibitors p53, p21 and prb. Here, we report the characterization of MSNs toxicity and cellular uptake in cardiomyocytes. References [1] Hernandez-Segura, A., Nehme, J., and Demaria, M. (2018). Hallmarks of Cellular Senescence. Trends Cell Biol. Feb 21 doi: /j.tcb [2] Petrova, N. V., Velichko, A. K., Razin, S. V. and Kantidze, O. L. (2016), Small molecule compounds that induce cellular senescence. Aging Cell, 15: doi: /acel [3] Piegari E, De Angelis A, Cappetta D, Russo R, Esposito G, Costantino S, Graiani G, Frati C, Prezioso L, Berrino L, Urbanek K, Quaini F, Rossi F (2013) Doxorubicin induces senescence and impairs function of human cardiac progenitor cells. Basic Res. Cardiol. 108, 334. doi: /s [4] Agostini, A., Mondragón, L, Bernardos, A, Martínez Máñez, R, Marcos, M. D., Sancenón, F, Soto, J, Costero, A, Manguan García, C. Perona, R. Moreno Torres, M, Aparicio Sanchis, R. and Murguía, J. R. (2012), Targeted Cargo Delivery in Senescent Cells Using Capped Mesoporous Silica Nanoparticles. Angew. Chem. Int. Ed., 51: doi: /anie

131 P-85 ESSENTIAL OIL COMPONENTS LOADED INTO NANOCLAYS AGAINST ASPERGILLUS NIGER AND STAPHYLOCOCCUS AUREUS Andrea Bernardos 1,2,4, Silvia Alvarez 4, Matej Bozik 4, Édgar Pérez-Esteve 1,3, Pavel Kloucek 4, Adela Frankova 4, Ramón Martinez-Manezc 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM). Universitat Politècnica de València, Universitat de València. Camino de Vera s/n, 46022, Valencia (Spain). anberba@upvnet.upv.es 2 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). 3 Grupo de Investigación e Innovación Alimentaria (CUINA), Universitat Politècnica de València, Camino de Vera s/n, E Valencia, (Spain). 4 Department of Quality of Agricultural Products, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, Prague 6-Suchdol, , (Czech Republic). 3 keywords: essential oil, nanoclays, antimicrobial agents Essential oil components (EOCs) are potent natural antimicrobial agents [1], however, their high volatility and reactivity limits their possible applications in many fields. In this context we report herein the preparation of a series of nanomaterials containing EOCs (i.e. allyl isothiocyanate, carvacrol, cinnamaldehyde, diallyl disulfide, eugenol, thymol, and thymoquinone). The materials were prepared by simple adsorption of the essential oils in a montmorillonite nanoclay. The antimicrobial activity of these supports was studied against Aspergillus niger and Staphylococcus aureus, used as models of agricultural fungal and foodborne bacterial pathogens, respectively. Besides the activity of the encapsulated essential oils was compared with that of pure compounds. Enhanced antifungical and antibacterial activity of encapsulated EOCs when compared with the corresponding unencapsulated substances were observed. The results obtained indicated that the encapsulation of essential oil components into low cost nanoclays has great potential as antimicrobials when compared with the use of the pure components. See Figure 1. Figure 1. FESEM image of (a) MMT and (b) MMT-Eu showing the typical structure of MMT. References [1] A. Bernardos, T. Marina, P. Zacek, E. Perez-Esteve, R. Martinez-Manez, M. Lhotka, L. Kourimska, J. Pulkrabek, P. Kloucek. J. Sci. Food Agric. 2015, 95(14),

132 P-86 BLU-RAY TECHNOLOGY FOR CLASSIFYING PATIENTS WITH CARDIOVASCULAR DISEASES Ana Lázaro 1, Eric Yamanaka 1, Luis A. Tortajada-Genaro 1,2, Ángel Maquieira 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Camino de Vera s/n, E46022 Valencia, Spain 2 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain. anlaza@posgrado.upv.es keywords: Blu-ray technology, single nucleotide polymorphism, CYP2C9 Sensor systems based on compact disc technology can be used for molecular recognition into the surface of a standard compact disc combined with optical transduction [1]. In this sense, Blu-ray technology has exceptional possibilities as an interactive analytical tool, including microarray analysis [2]. In this communication, we reported a method capable of discriminating single nucleotide polymorphism using Blu-ray biosensing system. First, genomic DNA was extracted from buccal epithelium of volunteers, then we performed an allele specific ligation reaction followed by a universal amplification. The generated products were hybridized with probes immobilized on the Blu-ray discs and stained via immunoassay. Finally, the resulting arrays were read by the Blu-ray disc drive. Gray-scale images (Tagged Image File Format, color depth 16 bit) were generated, and the optical intensity signals of each spot were quantified using in-home software. Image processing (feature gridding, addressing, segmentation, and quality assurance) was automatically performed in under 5 min. The system showed excellent analytical performances and can be used in low-resource laboratories, due to its simplicity and low-cost, both in acquisition and maintenance. As proof of concept, we studied the discrimination of the genetic variants related to cardiovascular diseases. This experiment was focused on the adequate prescription of oral anticoagulants antagonists of vitamin K [3]. For that, the developed method was applied for the genotyping of a single nucleotide polymorphism in CYP2C9 gene. Since a correct discrimination of the allelic variants was achieved, a clear classification of the patients was obtained in population groups depending on the drug metabolic activity. These results demonstrated that Blu-ray technology can become a support tool for a better selection of drug doses depending on the individual genetic profile. Moreover, as this technology has important advantages, it is a promising alternative platform fulfilling the point-of-care demands of the current clinical practice. ACKNOWLEDGMENTS: Financial support from FEDER, GVA PROMETEO II /2014/040, BIOHOLOG (MINECO CTQ R) and PhD grant (GVA-FPI-2017). References [1] Morais S. Tortajada-Genaro LA, Arnandis Chover T, Puchades R, Maquieira A. Anal. Chem. 2009, 81, [2] Arnandis-Chover T, Morais S, González-Martí-nez MA, Puchades R, Maquieira A. Biosens. Bioelectron. 2014, 51, [3] Baker W, Johnson S. Curr Opin Pharmacol. 2016, 27,

133 P-87 ADVANCED BLOCKED DNA AMPLIFICATION OF MUTANT VARIANTS RELATED TO CANCER TREATMENT Ana Lázaro 1, Luis A. Tortajada-Genaro 1,2, Ángel Maquieira 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València-Universitat de València, Camino de Vera s/n, E46022 Valencia, Spain 2 Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, E46022 Valencia, Spain. anlaza@posgrado.upv.es keywords: Personalized medicine, blocker, minority alleles Personalized medicine aims to select the treatment according to the molecular characteristics of each patient, such as the mutational status of certain genes [1]. Thus, better therapeutic results are achieved, reducing undesirable side effects and economic costs [2]. But, the scientific-technical challenge is the correct discrimination of patients because the point mutations are generally low-level [3]. In this communication, we described a new approach of the blocked amplification of mutant variants in cancer tissues. In the conventional reaction, a synthetic oligonucleotide complementary to the wild type sequence is added in the amplification solution. So, its replication is avoided, enriching the minority alleles if they are present in the sample. In the new method, the recognition of blocking agent to the native sequence is improved before the primer is attached. We have determined an additional incubation step (temperature 72 C, time 1 min). Also, we have studied the concentration and nature of blockers, such as functionalization with the amino group. Real-time measurements have showed the preliminary results related to the blocking yield. The detection of mutant variants in mutant type/wild wide DNA mixtures is under study. An important selective increase the copy number of minority alleles is expected. In case of success, advanced blocked DNA amplification would have the potentiality to support the future development of a routine test that would provide a personalized prognosis. ACKNOWLEDGMENTS: Financial support from FEDER, GVA PROMETEO II /2014/040, BIOHOLOG (MINECO CTQ R) and PhD grant (GVA-FPI-2017). qpcr measures were performed with the collaboration of the UPV Animal Science department. References [1] Lièvre et al. J Clin Oncol. 2008, 26(3): [2] Gonzalez-Angulo et al. J Clin Oncol. 2010, 28(16), [3] Douillard et al. N Engl J Med. 2013,12;369(11):

134 P-88 Detección de catecolaminas haciendo uso de derivados de BODIPYs Estefanía Almenar 1, Ana M. Costero 1,2,3, Pablo Gaviña 1,2,3, Salvador Gil 1,2,3 y Margarita Parra 1,2,3 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat de València-Universitat Politècnica de València, Valencia, Spain, dirección 1, del autor presentador 2 Departamento de Química Orgánica, Universitat de València, Doctor Moliner 50, 46100, Burjassot, Valencia, Spain 3 CIBER de Bioingeniería Biomateriales y Nanomedicina (CIBER-BBN), Spain Palabras clave: Aminas biógenas, BODIPY, Detección Fluorocromogénica Las aminas biógenas endógenas (como la adrenalina, noradrenalina y la dopamina) realizan múltiples funciones, principalmente como neurotransmisores y como hormonas, en el cuerpo humano. Es por ello que su detección resulta útil para el control de enfermedades en las que se dan niveles anormales de estas: Parkinson, Alzheimer o Esquizofrenia. HO NH 2 HO OH NH 2 HO OH H N HO HO HO Dopamina Noradrenalina Adrenalina Figura 1: Aminas biógenas. Los BODIPYs han demostrado ser de gran utilidad en la detección de distintos analitos debido a sus propiedades fluorogénicas y cromogénicas que se pueden modular mediante la introducción de diversos grupos funcionales. En el presente trabajo, se quiere detectar las aminas biógenas: adrenalina, noradrenalina y dopamina; utilizando un sensor con un BODIPY funcionalizado con dos grupos distintos capaces de interaccionar por un lado con el catecol y por otro lado con el grupo amino o aminoalcohol presentes en estas aminas biógenas. Es de esperar que la formación de un complejo con las catecolaminas module las propiedades fluorocromogénicas del BODIPY. MeO N N B F F N NH HO B HO OH HO O Referencias [1] Cheng-Hao Liu, Cheng-Ju, Wei-Lung Tseng. Anal. Chim , vol.,

135 P-89 Nanodevices for the efficient co-delivery of CRISPR-Cas9 editing machinery and an entrapped drug to solve inflammatory disorders Alba García-Fernández 1,3,4, Gema Vivo Llorca 1,3,4, Mónica Sancho 4,5, Félix Sancenón 1,2,3,4, José Ramón Murguía 1,3, Ramón Martínez-Máñez 1,2,3,4 and Mar Orzáez 4,5 1Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València. Spain. algarfe4@etsia.upv.es 2Departamento de Química, Universitat Politècnica de València, Camí de Vera s/n, 46022, València, Spain. 3CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) Spain. 4 Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Valencia, Universitat Politècnica de València, Centro de Investigación Príncipe Felipe, València, Spain. 5Centro de Investigación Príncipe Felipe, Laboratorio de Péptidos y Proteínas, València, Spain 3 keywords: CRISPR_MSNs, Gasdermin D, Inflammasome The recently development of the CRISPR/Cas9 technology represents a major advance in the genetic engineering field. This is a genome-editing system, formed by two main components; the non-specific CRISPR-associated endonuclease (Cas9) and guide RNA (grna).grna directs the Cas9 endonuclease to produce targeted double-stranded breaks in chromosomes that can be repaired by either non-homologous end joining or by homologous recombination [1,2]. Despite the huge potential of the CRISPR technology in basic research and potential therapeutics for genome regulation, the efficient delivery of CRISPR/Cas9 systems to cells remains challenging [3]. In this work, as a proof of concept, we report a nanodevice capable of delivering the CRISPR-Cas9 editing machinery and simultaneously releasing an entrapped cargo. The system is based on mesoporous silica nanoparticles loaded with rhodamine B (as a model drug), functionalized with PEI and finally capped with the CRISPR Cas9 vector (to edit the GFP gene). The gene editing potential of nanoparticles is verified by knocking down the gene expression of green fluorescent protein by ca. 45% in U-2 OS-GFP cells. The co-delivery of rhodamine B as a result of pore opening is also verified. Taken together, our results show the potential of preparing advanced nanodevices for disease treatment by co-delivering drugs and gene editing machinery for possible applications. Based on this concept, we could implement one-shot treatments to simultaneously edit genes and release drugs by double-hit strategies. In this case, we are focused in the resolution of inflammation disorders. MSNs can be design to release an anti-inflammatory drug, whose effect is complemented or potentiated by the CRISPR-Cas9 gene editing. The inflammatory process is characterized by the activation and release of inflammatory cytokines, and also by activation of the inflammatory cell death called pyroptosis. Anti-inflammatory drugs, such as VX-765, only act inhibiting the activity of certain cytokines but not the process of pyroptosis [4]. Recently, a protein involved in the process of pyroptosis activation, Gasdermin D (GSDMD), was described [5]. For this reason, we want to combine in CRISPR_MSNs, the effect of the anti-inflammatory drug VX-765 and the editing of GSDMD to deal with the resolution of inflammation. References [1] F. J. M. Mojica, L. Montoliu, Trends Microbiol. 2016, 24, [2] J. A. Doudna, E. Charpentier, Science 2014, 346, [3] P. D. Hsu, E. S. Lander, F. Zhang, Cell 2014, 157, [4] W. Wannamaker, R. Davis, M. Namchuk, J. Pollard, P. Ford, G. Ku, C. Decker, P. Charifson, P. Weber, U.A. Germann, K. Huida, J.C.R. Randle, J. Pharmacol. Exp. Ther, 2007, 321, [5] X. Liu, Z. Zhang, J. Ruan, Y. Pan, V.G. Magupalli, H. Wu and J. Lieberman. Nature. 2016, 535,

136 P-90 PROTOCELLS SKIN PERMEABILITY: A FIRST STEP TO DEVELOP A NOVEL ANTI-AGING NANOSYSTEM Adrián H Teruel* 1,2, I. González-Álvarez 3, V. Merino-Sanjuán 1,4, F. Sancenón 1,2,5,6, R. Martínez-Máñez 1,2,5,6 and M. González-Álvarez 3 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Spain. * Contact adherte@upv.es 2 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). 3 Departamento de Ingeniería, Sección de Farmacia y Tecnología Farmacéutica, Universidad Miguel Hernandez, 03550, Alicante, Spain. 4 Departament de Farmacia i Tecnologia Farmacèutica, Universitat de València 46100, Valencia, Spain. 5 Unidad Mixta de Investigación en Nanomedicina y Sensores. Universitat Politècnica de València, Instituto de Investigación Sanitaria La Fe, Valencia, Spain 6 Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Valencia, Universitat Politècnica de València, Centro de Investigación Príncipe Felipe, Valencia, Spain Keywords: mesoporous silica nanoparticles, lipid bilayer gate, antioxidants Delivery of active ingredients through the skin is still a challenge when sustained release and high concentration are required in the living skin cells layers to achieve high efficacy. Furthermore, ingredients with poor solubility or unstable (that can be easily oxidized or disrupted by several factors, such as O 2, UV radiation, heat, ) will need a framework or a vehicle to increase their concentration and to protect them before to carry out their commitment. Researchers are increasingly studying the synthesis and use of novel nanostructures that efficiently encapsulate drugs at high concentration, cross the cell membranes, and allow sustainable release of their payload, but challenges like biocompatibility and high load capacity are not yet achieve. Here we report the results of a comparative skin permeability study (by means of Franz cell diffusion technique) of two nanosystems (S1 and S2) using two different solvents (HBSS or PPG). S1 consists in MCM-41-like mesoporous silica nanoparticles (MCM-41- type MSNs, S0) covered with a lipid bilayer protocells. Pore size 2-3 nm. S2 consists in dendrimer-like mesoporous silica nanoparticles (DMSNs, S0 ) covered with a lipid bilayer. Pore size 20+/-12 nm.[1,2] Both systems are loaded with curcumin, a poor water soluble and heat sensitive but potent antioxidant with promising utility in the antiaging and anti-inflammatory fields. Those systems were designed to act as a vehicle, enhancing the permeability of the active (curcumin) entrapped in the mesoporous silica core, due to its nanometric size and the fusion of the lipid bilayer with the epidermal and dermal membrane cells. At the same time the silica framework should protect the active improving its stability (this last is not reflected in this work). References [1] Liu J, Stace-Naughton A, Jiang X, Brinker CJ. J Am Chem Soc. 2009, 131, [2] Du X, Xiong L, Dai S, Kleitz F, Zhang Qiao S. Adv Funct Mater. 2014, 24,

137 P-91 Selective colorimetric detection of norepinephrine through specific ligand recognition using functionalized gold nanoparticles Tania Godoy 1,2, Pablo Gaviña 1,2, Ana Costero 1,2, Ramón Martínez 1,2, and Félix Sancenón 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Spain, tago@upvnet.upv.es 2 CIBER de Bioingenierıía, Biomateriales y Nanomedicina (CIBER-BBN), Spain. Norepinephrine, neurotransmitter, gold nanoparticles Scheme 1. Sensing paradigm of the colorimetric detection of norepinephrine. Norepinephrine (NE), also called noradrenaline, acts as a hormone as well as a neurotransmitter and is commonly known as the hormone of the stress. NE is released from noradrenergic neurons in the central and sympathetic nervous system. It has been closely associated with some physiological processes such as stress, anxiety, sleep, and memory. [1] NE also plays a key role in the fight-or-flight response. Low levels of NE have been reported in patients with pathologies, such as depression,[2] Alzheimer,[3] and Parkinson's disease.[4] Conversely, high levels of NE are indicative of cancerous tumours such as pheochromocytoma and paraganglioma, which start in the medulla of the adrenal glands, secreting large amounts of NE.[5] Due its great potential as a biomarker in the early diagnosis of the aforementioned pathologies, the design and development of simple, fast and effective methods for detection and quantification of NE are of importance. Therefore a colorimetric method based on gold nanoparticles was designed. The colorimetric probe (P1) consists of gold nanoparticles functionalized with 4-(liponyloxy)benzaldehyde (L1) and 4- mercatophenylboronic acid (L2) Scheme 1. These aldehyde and boronic acid-terminated ligands were selected to react with the aminoalcohol and catechol moieties of NE by forming the corresponding oxazolidine and boronate derivatives respectively. This double interaction leads to interparticle crosslinking aggregation resulting in a colour change of the solution, from red to blue, clearly visible to the naked eye. The probe is highly selective towards NE, and very little or no response at all was observed in the presence of other catecholamines. References [1] A. Goddard, S. Ball, J. Martinez, M. Robinson, C. Yang, J. Russell, and A. Shekhar, Anxiety 2010, 27, [2] D. J. Nutt, J Clin Psychiatry 2006, 67, 3-8. [3] T. Chalermpalanupap, B. Kinkead, W. Hu, M. Kummer, T. Hammerschmidt, M. Heneka, D. Weinshenker and A. I. Levey,, Alzheimers Res. Ther. 2013, 5, 21. [4] C. Delaville, P. De Deurwaerdère, and A. Benazzouz, Front. Syst. Neurosci. 2011, 5, 31. [5] V. Kantorovich and K. Pacak, Prog. Brain Res. 2010, 182,

138 P-92 L-Glutamate-responsive nanocarrier based on Janus Au mesoporous silica nanoparticles Tania M. Godoy-Reyes 1,2, Antoni Llopis-Lorente 1,2, Pablo Gaviña 1,2, Ana Costero 1,2, Reynaldo Villalonga, 3 Ramón Martínez 1,2, and Félix Sancenón 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Spain, tago@upvnet.upv.es 2 CIBER de Bioingenierıía, Biomateriales y Nanomedicina (CIBER-BBN), Spain. 3 Departamento de Química Analítica, Facultad de Química, Universidad Complutense de Madrid, Madrid (Spain) Keywords: Glutamate, controlled release, mesoporous materials L-glutamate is the most abundant excitatory neurotransmitter in the central nervous system. Due to its role in synaptic plasticity, it is involved in cognitive functions such as learning and memory in the brain [1]. However, the excitotoxicity produced by the excessive glutamate release occurs as part of the ischemic cascade and is associated with stroke,[2] autism, some forms of intellectual disability, and diseases such as amyotrophic lateral sclerosis, lathyrism, and Alzheimer's disease.[3] In the recent years, the development of better therapies for the treatment of the above mentioned pathologies is receiving special attention. Therefore we have developed a glutamate oxidase-controlled nanodevice based on Janus-type Au mesoporous silica nanoparticles for triggered release in response to L-glutamate (Figure 1). The enzyme glutamate oxidase is immobilized in the Au face of the Janus nanoparticle and acts as a "control unit". The mesoporous silica nanoparticles face is loaded with a cargo ([Ru(bpy) 3 ]Cl 2) and capped with a self-immolative gate that responds to hydrogen peroxide. In the presence of L-glutamate, it is oxidized by the enzyme glutamate oxidase to give 2-oxoglutarate, NH 3 and H 2 O 2 which induces a self-immolative degradation of the gate, and therefore the release of the cargo. References [1] G. Riedel, B. Platt, and J. Micheau, Behav Brain Res, 2003, 14, [2] A. Dávalos, J. Castillo, J. Serena, and M. Noya, Stroke, 1997, 28, [3] D. Choi, Neuron, 1988, 1, Figure 1. Representation of the L-glutamateresponsive delivery system.

139 P-93 OLEIC ACID-CAPPED MESOPOROUS SILICA PARTICLES WITH DIFFERENT POROUS SIZES Elisa Poyatos-Racionero 1,2, Édgar Pérez-Esteve 1,3, Andrea Bernardos 1,2, Elena Aznar 2,1, María Dolores Marcos 1,2, Ramón Martinez-Mañez 1,2 1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM). Universitat Politècnica de València, Universitat de València. Camino de Vera s/n, 46022, Valencia (Spain). elpora@upvnet.upv.es 2 CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). 3 Grupo de Investigación e Innovación Alimentaria (CUINA), Universitat Politècnica de València, Camino de Vera s/n, E Valencia, (Spain). Keywords: oleic acid, mesoporous silica particles, controlled release One of the goals of current medicine is the controlled release of substances in specific points of the organism, to be able to decide the dose and the time of administration. Mesoporous materials, due to their versatility, are a promising candidate to contain these molecules; and thanks to its easy functionalization process, to be directed towards the focus of action. This is a relatively developed field, so there are multiple mesoporous materials that differ in their composition, shape, size and pore volume; which opens a range of possibilities for encapsulating molecules of different dimensions. This work was focused on the use of oleic acid as a molecular gate in different types of mesoporous materials in a fast, easy and effective way, capping in all of them the pores despite their different sizes. The particles functionalized with oleic acid have a strongly hydrophobic layer around them due to the nature of the fatty acid. This layer avoids the interaction between cargo and environmental water molecules, acting as closed gate. Only surfactant molecules can open the gate due to their emulsifier action on the hydrophobic layer. This opening mechanism can be used in food field and even in the release of drugs in the small intestine, using the surfactant effect of bile salts. The different pore size of the materials compared in this study, and the different release profile may be reasons for selecting one or another material to keep and release a specific molecule. 139

140 P-94 Conductivity study of phosphoric-doped composite Polybenzimidazole membranes with ionic liquids for high temperature fuel cells applications Abel Garcia-Bernabé 1, Jorge Escorihuela 1, Arturo Barjola-Ruiz 1, Álvaro Montero 1, Óscar Sahuquillo 2, Enrique Gimenez 2, Vicente Compañ 1 1 Departamento de Termodinámica Aplicada, Universitat Politècnica de València, camino de Vera s/n, Valencia (Spain) 2 Instituto de Tecnología de Materiales, Universitat Politècnica de València, camino de Vera s/n, Valencia (Spain) Proton Transport; PBI; Ionic Liquids. Polybenzimidazole (PBI) is a substitute for Nafion membrane as a Proton Exchange Membranes (PEM) in application above 120ºC without external humidification. PBI was first proposed for preparing phosphoric-doped membranes [1]. These anhydrous membranes allow decrease the poisoning by CO and membrane swelling and water management will not be limiting factors for such membranes [2]. A serie of composite membranes based on phosphic-doped-pbi with 1-butyl-3- methtlimidazolium and different anions ionic liquids (BMIM-X) with a concentration of ionic liquid of 5% wt in all the membranes. The conductivity of the synthesized membranes was determined by electrochemical impedance spectroscopy (EIS) over a range of frequencies (from 0.1 to 10 MHz). These membranes displayed good proton conductivity in wet and dry conditions, and the temperature dependence shown different behaviour in wet and dry conditions. This proton conductivity depends on the anion of ionic liquid. Acknowledgments This research has been supported by ENE/ R project, granted by the Ministerio de Economica y Competitividad (MINECO), Spain. References [1] Li Q., Jensen J.O., Savinell R.F. and Bjerrum N.J., High temperature proton exchange membranes based on polybenzimidazoles, Progress in Polymer Sci. 34 (2009) [2] Devanathan R., Recent developments in proton exchange membranes for fuel cells, Energy Environ. Sci. 1 (2008)

141 P-95 MIXED MATRIX MEMBRANES OF SPEEK WITH ZEOLITIC IMIDAZOLATE FRAMEWORKS FOR PROTON CONDUCTING APPLICATIONS Abel García-Bernabé 1, Arturo Barjola 1, Vicente Compañ 1, Enrique Giménez 2,Jorge Escorihuela 1,Oscar Sahuquillo 2 1 Departamento de Termodinámica Aplicada, Escuela Técnica Superior de Ingenieros Industriales (ETSII), Universidad Politécnica de Valencia, Campus de Vera s/n, 46020, Valencia, Spain 2 Departamento de Ingeniería de Materiales, Universidad Politécnica de Valencia, Valencia, Spain Zifs,conductivity,membranes Sulfonated poly(ether-ether-ketone) (SPEEK) is a promising material for use like a Polymeric Electrolyte Membrane Fuel Cells (PEMFC). This polymer has a good proton conductivity and their T g is around 200 C [1]. Although it is known that the main problem with this material is its solubility in water at high sulfonation degrees [2] and therefore it is necessary to stabilize it for use on a PEMFC. In this regard, composites of SPEEK with different Zeolitic Imidazolate Frameworks (ZIFs) have been investigated. ZIFs with Zn (ZIF 8) or Co (ZIF67), and a mix from both (Zn and Co) in a 1:1 (wt) proportion were synthesized and used like a fillers in polymeric SPEEK membranes. Polymeric electrolyte membranes of SPEEK with different amounts of ZIFs in DMAc were prepared by solution casting method. The membranes were studied the morphology by Scanning Electron Microscope (SEM), the thermal stability by thermogravimetric analysis (TGA) and the conductivity by means of impedance spectroscopy analysis in the frequency range of 10-1 < f < 10 7 Hz applying a 0.1V signal amplitude. The results indicate that SPEEK membranes doped with ZIFs improve the conductivity of pristine SPEEK membranes and they have shown a great potential as ion-exchange membranes for fuel cell applications. Figure 1. Real part of conductivity and phase angle for Mixed ZIF 1% in the range of temperatures from 20 C to 140 C and real part of conductivity for Acknowledgments This research has been supported by ENE/ R project, granted by the Ministerio de Economica y Competitividad (MINECO), Spain. References [1] S.Mollà, N. V.Compañ. International Journal of Hydrogen Energy. 2014, [1] Huang RYM, Shao P,Burns CM,Feng X. Journal applied polymers science. 2001,