PROGRAM. & 4 th International ssdna Comparative Virology Workshop. Guanajuato, Guanajuato, México November 7 12, 2010

Transcripción

1 6 th International Geminivirus Symposium & 4 th International ssdna Comparative Virology Workshop PROGRAM Guanajuato, Guanajuato, México November 7 12, th International Geminivirus Symposium i

2 Editors Committee Organizing Committee National Rafael Rivera-Bustamante Diana L. Trejo Saavedra Luis Pérez Moreno Irineo Torres-Pacheco Ramón Guevara-González Oscar Moreno Valenzuela Jesús Méndez-Lozano J.A. Garzón-Tiznado Rodolfo de la Torre-Almaraz Gerardo Argüello-Astorga Raúl Díaz-Plaza Jaime Holguín-Peña Organizing Committee International J.T. Ascencio-Ibañez Linda Hanley Bowdoin Claude Fauquet Judith K. Brown F. Murilo Zerbini Ed Rybicki Eduardo R. Bejarano Rob Briddon Gian Paolo Accotto Philippe Biagini Xian-Jim Meng Peter Tijssen Siobain Duffy ii 4 th International ssdna Comparative Virology Workshop

3 6 th International Geminivirus Symposium 4 th International ssdna Comparative Virology Workshop November 7 12, 2010 Guanajuato, México Welcome to Guanajuato: Three years ago en Ouro Preto, Brazil, you decided to come to Mexico. During the presentation I felt that the slide that was most appreciated was: Why Mexico? Because we want you there!. Indeed, we are very happy that you are coming to Guanajuato, Mexico for the 6th International Geminivirus Symposium and the 4th International ssdna comparative virology Workshop. Welcome. What is the formula, the recipe to make a scientific meeting a success? The first and key ingredient that has been always present in the previous Symposia: an excellent academic program. And that means you. We have registered more than 150 attendants from all around the World, we have scheduled more than 30 keynote / plenary talks and have received more than 100 abstracts. The contributions received for this meeting reflect interesting, informative major tendencies on ssdna virology research. Virus-host interaction and virus diversity / evolution are favorite research areas this year. We will be presented also with new methodologies and technological platforms that are helping us to generate more data than ever, that facilitate new discoveries. In my opinion this is excellent news. Yes, we are characterizing new viruses, detecting new pathogenic entities, and learning how these pathogens evolve, and interact among themselves But we are also learning like never before how these entities interact with the host cells, with their machinery, and at the end, how they cause disease. Which is probably what the general population is interested in. As in many other scientific disciplines, bioinformatics is becoming a major player in our research, not only to analyze and compare the now hundreds of virus genomes, but also how to assemble new genomes without cloning them, how to trace back an important pathogen to its origins, and even how to understand the meaning of hundreds of genes that are over- or under-expressed in a infected host cell. Finally, another learning experience from analyzing geminivirus literature is that research has become more and more an interdisciplinary and collaborative activity. We hope that this meeting will be the origin, the source, a melting-pot, for new and productive collaborations that will benefit everybody. To those with years of experience, let us show the students, those that represent the next generations, the advantages of collaboration and coordinated efforts. That can be also an important legacy. Enjoy Guanajuato and remember this is also a Birthday Party. Rafael Rivera-Bustamante For the organizing committee. 6 th International Geminivirus Symposium iii

4 Funding Institutions iv 4 th International ssdna Comparative Virology Workshop

5 Sponsors 6 th International Geminivirus Symposium v

6 Table of Contents Schedule... viii Program of Events Sunday... 3 Monday... 4 Tuesday... 7 Wednesday Thursday Friday Poster Sessions Author Index vi 4 th International ssdna Comparative Virology Workshop

7 6th International Geminivirus Symposium, November 7-12, Guanajuato, Mexico PLENARY SESSION 1 ENRIQUE MORIONES 9:00 9:30 PLENARY SESSION 2 J C FARIA 9:30 10:00 10:00 10:30 COFFEE BREAK COFFEE BREAK COFFEE BREAK COFFEE BREAK COFFEE BREAK ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS 10:30 12:30 NEXT MEETING PRESENTATION AND CLOSING REMARKS 12:30 14:00 LUNCH LUNCH LUNCH LUNCH LUNCH SUNDAY NOV. 7 MONDAY TUESDAY WEDNESDAY THURSDAY FRIDAY NOV. 8 NOV. 9 NOV. 10 NOV. 11 NOV. 12 7:30 9:00 BREAKFAST BREAKFAST BREAKFAST BREAKFAST BREAKFAST PLENARY TALKS DISEASE, EPID. AND DISEASE CONTROL 9:00 10:00 PLENARY TALKS RECENT ADVANCES IN SSDNA RESEARCH EDUARDO R. BEJARANO KEYNOTE SPEAKER PLENARY TALKS VIRUS HOST INTERACTION I LINDA HANLEY-BOWDOIN KEYNOTE SPEAKER PLENARY TALKS VIRUS HOST INT. II ETHEL-MICHELE DE VILLIERS KEYNOTE SPEAKER PLENARY TALKS VIRUS DIVERSITY/ EVOLUTION HOLGER JESKE KEYNOTE SPEAKER 10:30 11:00 PLENARY SESSION 1 PHILIPPE BIAGINI PLENARY SESSION 1 DAVID BISARO PLENARY SESSION 1 ELIZABETH FONTES PLENARY SESSION 1 JUDITH K BROWN 11:00 11:30 PLENARY SESSION 2 PETER TIJSSEN PLENARY SESSION 2 SONDRA LAZAROWITZ PLENARY SESSION 2 SUNIL K MUKHERJEE PLENARY SESSION 2 ROB BRIDDON 11:30 12:00 PLENARY SESSION 3 H. JOSEF VETTEN PLENARY SESSION 3 XUEPING ZHOU PLENARY SESSION 3 BRUNO GRONENBORN PLENARY SESSION 3 F MURILO ZERBINI 12:00 12:30 PLENARY SESSION 4 CLAUDE FAUQUET PLENARY SESSION 4 CHRISTINA WEGE PLENARY SESSION 4 TBA PLENARY SESSION 4 DARREN MARTIN REGISTRATION 14:00 TO 18:30 14:00 16:00 WORKSHOP TALKS EMERGING / NOVEL VIRUSES WORKSHOP TALKS VIRUS HOST INTERACTION II FREE AFTERNOON WORKSHOP TALKS VIRUS DIVERSITY AND EVOL 14:00 14:30 PLENARY SESSION 5 MYA BREITBART PLENARY SESSION 5 GIAN PAOLO ACCOTTO SPECIAL SSDNA TAXONOMY WORKSHOP PLENARY SESSION 5 CLAUDE FAUQUET

8 6th International Geminivirus Symposium, November 7-12, Guanajuato, Mexico FRIDAY NOV. 12 SUNDAY NOV. 7 MONDAY NOV. 8 TUESDAY NOV. 9 WEDNESDAY NOV. 10 THURSDAY NOV :30 15:00 PLENARY SESSION 6 ARVIND VARSANI PLENARY SESSION 6 RAFAEL RIVERA-BUSTAMANTE PLENARY SESSION 6 SIOBAIN DUFFY 15:00 15:45 ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS 16:00 16:30 COFFEE BREAK COFFEE BREAK COFFEE BREAK 16:30 18:30 WORKSHOP TALKS VIRUS HOST INTERACTION I WORKSHOP TALKS VIRUS REPLICATION GENE EXP. WORKSHOP TALKS VIRUS VIRUS DIV. / EVOL. 16:30 17:00 17:00 18:15 PLENARY SESSION 7 DOMINIQUE ROBERTSON ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS PLENARY SESSION 7 GARRY SUNTER PLENARY SESSION 8 MIKHAIL POOGGIN 17:00 17:30 ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS 16:30 18:15 ORAL SESSIONS FOR PRESENTATIONS CHOSEN FROM ABSTRACTS 17:30 18:15 WELCOME COCTAIL 18:30 TO 20:30 18:30 20:00 POSTER SESSION ODD NUMBERS POSTER SESSION EVEN NUMBERS 21:00 FOLKLORIC BALLET CALLEJONEADA GALA DINNER

9 PROGRAM OF EVENTS

10 Sunday November 7, :00 to 20:00 Registration San Gabriel Room 18:30 20:30 Welcome Cocktail All oral presentations will be held in the La Mancha Room All poster presentations will be held in the La Mancha Room 6 th International Geminivirus Symposium 3

11 Monday November 8, 2010 Plenary Talks Recent Advances in ssdna Research 9:00 10:00 Keynote Address Eduardo Bejarano IHSM UMA CSIC, Spain Geminiviral avoidance and exploitation of the posttranslational systems 10:00 10:30 Coffee Break 10:30 11:00 11:00 11:30 11:30 12:00 12:00 12:30 Philippe Biagini Université de la Méditerranée, France Taxonomy of Anelloviridae and Circoviridae: past, present, future Peter Tijssen Université du Québec, Canada Parvoviridae: small viruses, big surprises H. Josef Vetten Julius Kühn Institute, Germany Recent advances in the study of viruses of the family Nanoviridae Claude Fauquet ILTAB Danforth Center Ever increasing biodiversity of single stranded DNA viruses 12:30 14:00 Lunch 4 4 th International ssdna Comparative Virology Workshop

12 Monday November 8, 2010 Workshop Talks Emerging / Novel Viruses 14:00 14:30 14:30 15:00 15:00 15:15 15:15 15:30 15:30 15:45 15:45 16:00 Mya Breitbart - Plenary University of South Florida, USA Discovery of Novel Plant and Animal Viruses Through Vector-Enabled Metagenomics on Whiteflies and Mosquitoes Arvind Varsani - Plenary University of Canterbury, University of Cape Town, South Africa Gemini-r-evolution: Discovery of novel viruses and insights into the interplay between viruses infecting cultivated and indigenous plants Huma Muntaz NIBGE Pakistan Diversity of Dicot-infecting Mastreviruses Karyna Rosario University of South Florida, USA Discovery of a novel single-stranded DNA virus in dragonflies Cecilia Hernández The University of Arizona, USA Characterization of two newly discovered curtoviruses isolated from spinach in south-central Arizona Jesús Navas-Castillo IHSM UMA CSIC, Spain Genetic diversity of DNA satellites associated with Ipomoeainfecting monopartite begomoviruses 16:00 16:30 Coffee Break 6 th International Geminivirus Symposium 5

13 Monday November 8, 2010 Workshop Talks Virus Host Interaction I 16:30 17:00 17:00 17:15 17:15 17:30 17:30 17:45 17:45 18:00 18:00 18:15 Dominique Robertson - Plenary North Carolina State University, USA Gemini-VIGS in cotton Bernardo Bañuelos IPICyT, Mexico Two strains of Euphorbia mosaic virus are incompatible in replication and contain short Rep sequences in the BV1 promoter region Raúl Álvarez-Venegas CINVESTAV IPN Irapuato, Mexico Use of Virus-Induced Gene Silencing (VIGS) to accelerate genetic gain Asako Uchiyama Cornell University, USA Arabidopsis synaptotagmins: their roles in virus cell-to-cell movement and plant development J. Trinidad Ascencio-Ibañez North Carolina State University, USA BCTV and CaLCuV display a different interaction with the cell cycle machinery in Arabidopsis Jyothna P Indian Agricultural Research Institute, India Characterization of recovery phenomenon in Tomato leaf curl New Delhi virus : Correlation between viral DNA, transcripts and mirna levels 18:30 20:00 Poster Session (Odd-numbered posters) 6 4 th International ssdna Comparative Virology Workshop

14 Tuesday November 9, 2010 Plenary Talks Virus Host Interaction 9:00 10:00 Keynote Address Linda Hanley-Bowdoin North Carolina State University, USA Dissecting geminivirus/host interactions in Arabidopsis 10:00 10:30 Coffee Break 10:30 11:00 11:00 11:30 11:30 12:00 12:00 12:30 David Bisaro The Ohio State University, USA RNA-directed methylation targeted to geminivirus genomes: Defense and counter-defense Sondra Lazarowitz Cornell University, USA Plant cell-to-cell trafficking: Viruses touch a nerve Xueping Zhou Zhejiang University, P R China Tomato SlSnRK1 protein interacts with and phosphorylates geminivirus βc1 protein to attenuate viral infection Christina Wege University of Stuttgart, Germany Inter-species complementation of Old and New World begomoviruses in spread: "Artificial" two-component infections dissect virus-plant-interactions 12:30 14:00 Lunch 6 th International Geminivirus Symposium 7

15 Tuesday November 9, 2010 Workshop Talks Virus Host Interaction II 14:00 14:30 14:30 15:00 15:00 15:15 15:15 15:30 15:30 15:45 15:45 16:00 16:00 16:30 Gian Paolo Accotto - Plenary Istituto di Virologia Vegetale C.N.R, Italy Transcriptomic analysis of tomato infected by Tomato yellow leaf curl Sardinia virus Rafael Rivera-Bustamante - Plenary CINVESTAV IPN Unidad Irapuato, Mexico Characterization of a recovery stage in geminivirus-infected pepper plants: Transcriptomic analysis Imran Amin NIBGE - Pakistan Four suppressors of RNA silencing are encoded by cotton leaf curl Multan begomovirus complex Muhammad Mubin University of Agriculture Faisalabad, Pakistan The precoat protein encoded by a monopartite begomovirus is a suppressor ofboth post transcriptional and transcriptional gene silencing Edgar Rodríguez-Negrete IHSM, UMA-CSIC, Spain The Tomato yellow leaf curl virus, Sardinian isolate (TYLCSV) C4 protein is a putative transcriptional gene silencing (TGS) suppressor Pradeep Sharma Directorate of Wheat Research, India The V2 protein of Monopartite begomovirus determines virulence, hypersensitive response and suppression of posttranscriptional gene silencing Coffee Break 8 4 th International ssdna Comparative Virology Workshop

16 Tuesday November 9, 2010 Workshop Talks Virus Replication Gene Expression 16:30 17:00 17:00 17:15 17:15 17:30 17:30 17:45 17:45 18:00 Garry Sunter - Plenary The University of Texas, USA Functional studies of bipartite complementary sense promoters Mikhail Pooggin - Plenary University of Basel, Switzerland Primary and secondary sirnas in geminivirus induced gene silencing Björn Krenz University of Stuttgart, Germany The impact of geminiviral proteins on the nuclear architecture Aurora Londoño IPICyT, Mexico Identification of replication specificity determinants in eukaryotic ssdna viruses Poliane Alfenas-Zerbini Universidade Federal de Viçosa, Brazil APC7, a component of the Anaphase promoting complex, in begomovirus replication. 18:30 20:00 Poster Session (Even-numbered posters) 21:00 Callejoneada 6 th International Geminivirus Symposium 9

17 Wednesday November 10, 2010 Plenary Talks Virus Host Interaction II / Replication of ssdna viruses 9:00 10:00 Keynote Address Ethel-Michele de Villiers Division for the Characterization of Tumorviruses, Deutsches Krebsforschungszentrum, Germany The diversity of TT viruses: In vitro replication leads to the formation of virus-like particles and additional replication competent subviral molecules 10:00 10:30 Coffee Break 10:30 11:00 11:00 11:30 11:30 12:00 Elizabeth B. Fontes Universidade Federal de Viçosa, Brazil NSP-interacting kinase, NIK: a transducer of plant defense signaling suppressed by the begomovirus nuclear shuttle protein Sunil K. Mukherjee Plant Mol. Biol. Group, ICGEB, India Role of RAD54 protein in geminivirus DNA replication Bruno Gronenborn Institut des Sciences du Végétal, CNRS, France Variability and Evolution Rate of Plant Viruses with a Single- Stranded DNA Genome 12:30 14:00 Lunch Free Afternoon 10 4 th International ssdna Comparative Virology Workshop

18 Wednesday November 10, 2010 Special ssdna Taxonomy Workshop 15 minutes presentations Tax 1 Tax 2 Tax 3 Tax 4 Tax 5 Tax 6 Tax 7 Tax 8 Tax 9 A possible new taxonomy for the family Geminiviridae Claude Fauquet Divergent monopartitite geminiviruses: Eragrostis curvula streak virus and Turnip curly top virus Arvind Varsani A new species threshold for Mastreviruses Claude Fauquet A new taxa to accommodate two new Curtovirus species from Iran Arvind Varsani A new subgenus to accommodate the Swepoviruses Jesus Navas-Castillo and Judith K. Brown A new subgenus to accommodate the Legumoviruses and Copaviruses Claude Fauquet Alphasatellite taxonomy Rob Briddon, Claude Fauquet Betasatellite taxonomy Rob Briddon, Claude Fauquet Circovirus species threshold Arvind Varsani 6 th International Geminivirus Symposium 11

19 Thursday November 11, 2010 Plenary Talks Virus Diversity and Evolution 9:00 10:00 Keynote Address Holger Jeske Universität Stuttgart, Germany Circomics of geminiviruses 10:00 10:30 Coffee Break 10:30 11:00 11:00 11:30 11:30 12:00 12:00 12:30 Judith K. Brown The University of Arizona, USA The contribution of an alphasatellite-like molecule to symptom severity when in the presence of a begomovirus-beta satellite complex infecting tomato in Oman Rob W. Briddon NIBGE - Pakistan Betasatellites - then and now" F. Murilo Zerbini Universidade Federal de Viçosa, Brazil From 'wild' to 'domesticated' and back to 'wild': Genetic diversity and evolution of Brazilian begomoviruses Darren Martin University of Cape Town, South Africa Constraints on the adaptive value of recombination in ssdna viruses 12:30 14:00 Lunch 12 4 th International ssdna Comparative Virology Workshop

20 Thursday November 11, 2010 Workshop Talks Virus Diversity and Evolution 14:00 14:30 14:30 15:00 15:00 15:15 15:15 15:30 15:30 15:45 15:45 16:00 Claude Fauquet - Plenary ILTAB, USA High diversity and high infectiosity of geminiviruses and their associated satellites in symptomless reservoirs plants Siobain Duffy - Plenary Rutgers University, USA How do ssdna viruses evolve as quickly as RNA viruses? Elvira Fialló-Olivé Centro Nacional de Sanidad Agropecuaria, Cuba Begomovirus diversity and recombination in the Caribbean, a hot 'melting pot' in the New World Radha Anandalakshmi Mahyco Research Center, India Veni Vidi Vici - The tale of the Cotton leaf curl Burewala virus in India M. M. S. Almeida Universidade de Brasília / Embrapa Vegetables, Brazil Malvaceous plants are infected with novel Begomovirus species Jane Polston University of Florida, USA Characterization of a new virus discovered by vector-enabled metagenomics on the whitefly vector 16:00 16:30 Coffee Break 6 th International Geminivirus Symposium 13

21 Thursday November 11, 2010 Workshop Talks Virus Diversity and Evolution 16:30 16:45 16:45 17:00 17:00 17:15 17:15 17:30 17:30 17:45 17:45 18:00 Moshe Lapidot Volcani Center, Israel Use of rolling circle amplification to enable rapid completion of Koch s postulates for circular DNA viruses Supriya Chakraborty Jawaharlal Nehru University, India Genetic diversity and synergistic interaction among newly emerging begomoviruses associated with chilli leaf curl disease in India A. L. Monjane University of Cape Town, Observatory, South Africa Genetic analysis and phylogeographic history of MSV reveals the spatial and temporal origins of contemporary MSV-A recombinant lineages and identifies southern Africa as the ultimate origin of Maize streak disease Gordon W. Harkins South African National Bioinformatics Institute The spread of Tomato yellow leaf curl virus (TYLCV) from the Middle East to the world L. L. Esterhuizen University of Johannesburg, South Africa Epidemiology and molecular characterization of Tomato curly stunt virus and its insect vector Bemisia tabaci in South Africa T. G. Pereira Universidade de Brasília / Embrapa Vegetables, Brazil Begomovirus species diversity in South American weeds 21:00 Gala Dinner Sponsored by AgroBio-Mexico 14 4 th International ssdna Comparative Virology Workshop

22 Friday November 12, 2010 Plenary Talks Disease, Epidemiology and Disease Control 9:00 9:30 Enrique Moriones - Plenary IHSM-UMA-CSIC, Spain Integrated management of tomato yellow leaf curl disease 9:30 10:00 10:00 10:30 J. C. Faria Embrapa Arroz e Feijão, Brazil The epidemiology of Bean golden mosaic virus in two transgenic bean lines Coffee Break 10:30 10:45 10:45 11:00 11:00 11:15 11:15 11:30 L. Diniz-Mendez Instituto Oswaldo Cruz, FIOCRUZ, Brazil Assessment of human torque teno virus removal from two wastewater treatment plants Eybishtz Assaf The Hebrew University of Jerusalem, Israel A reverse genetics approach to discover tomato genes underlying resistance to TYLCV R. C. Pereira-Carvalho CNPH, Embrapa Vegetable Crops, IHSM, Brazil Segregation pattern of genetic resistance to Tomato yellow leaf curl virus - Israel (TYLCV-IL) in F2:3 families derived from the tomato hybrid TyKing Orlene Guerra-Peraza North Carolina State University, USA Quantitative trait loci analysis and whole genome sequences as tools to decipher the resistance to CaLCuV in the Pla-1 ecotype of Arabidopsis thaliana 6 th International Geminivirus Symposium 15

23 11:30 11:45 11:45 12:00 12:00 12:15 I. Levin The Volcani Center, Israel Molecular dissection of Ty-5, a Tomato yellow leaf curl virus resistance locus in the tomato line TY172 derived from Solanum peruvianum Ligia M. Almeida Embrapa Recursos Genéticos e Biotecnologia, Brazil RNAi-resistance to bipartite begomoviruses in genetically modified tomatoes Daisy Stainton University of Canterbury, New Zealand Genetic diversity of Banana bunchy top virus (BBTV) in the South Pacific Tongan archipelago 12:30 NEXT MEETING PRESENTATIONS AND CLOSING REMARKS 16 4 th International ssdna Comparative Virology Workshop

24 POSTER SESSIONS

25 POSTER SESSION ANELLOVIRIDAE Epidemiology 1 Genogroup distribution and viral load of Torquetenovirus in fecal samples. C. A. Pinho-Nascimento 1, J. P. G. Leite 2, C. Niel 1, L. Diniz-Mendes 1 1 Laboratório de Virología Molecular; 2 Laboratório de Virología Comparada e Ambiental, Instituto Oswaldo Cruz, Fiocruz, Avenida Brasil 4365, Rio de Janeiro, RJ, Brasil Replication 2 Transcription of TTV-HD types during in vitro virus replication. Jian-Wei Fei, Romana Kimmel and Ethel-Michele de Villiers Division for the Characterization of Tumorviruses, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 242, Heidelberg, Germany 3 Stimulation of TT replication by EBV in cells of lymphatic origin. Silvia S. Borkosky, Corinna Whitley, Annette Kopp-Schneider, Harald Zur Hausen and Ethel-Michele de Villiers Division for the Characterization of Tumorviruses, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 242, Heidelberg, Germany Other 4 Assessment of human torque teno virus removal from two wastewater treatment plants. L. Diniz-Mendes 1, C.A. Pinho-Nascimento 1, L.R. Bergsten-Torralba 1, V.S. De Paula, M.P. Miagostovich 2 and C. Niel 1 1 Laboratório de Virologia Molecular; 2 Laboratório de Virologia Comparada e Ambiental; 3 Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, FIOCRUZ Avenida Brasil, 4365 CEP Rio de Janeiro, RJ, Brasil. CIRCOVIRIDAE Genetic Diversity/Evolution 5 Origin and evolution of porcine circovirus type 2 in Cuba. 6 th International Geminivirus Symposium 19

26 POSTER SESSION Lester Josué Pérez, Heidy Díaz de Arce, Martí Cortey, Patricia Domínguez, Maria I. Percedo, Carmen Laura Perera, Joan Tarradas, Maria T. Frías, Joaquim Segalés, LLilianne Ganges, José Ignacio Núñez Centro Nacional de Sanidad Agropecuria (CENSA), La Habana, Cuba 6 Discovery of a novel single-stranded DNA virus in dragonflies. K. Rosario 1, M. Marinov 2, D. Stainton 2, S. Kraberger 2, D. Martin 3, M. Breitbart 1, and A. Varsani 2 1 College of Marine Science, University of South Florida, St. Petersburg, FL, USA, 2 School of Biological Sciences, University of Canterbury, Chirstchurch, New Zealand, 3 Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Rondebosch, South Africa 7 Identification of replication specificity determinants in eukaryotic ssdna viruses A. Londoño, L. Riego-Ruiz, G.R. Argüello-Astorga IPICyT, Camino a la Presa San José 2055, Colonia Lomas 4ta. Sección, San Luis Potosí, S.L.P., México C.P GEMINIVIRIDAE Taxonomy/Emerging viruses 8 Molecular characterization and phylogenetic relationships of two novel begomoviruses infecting weeds in Brazil. A.T.M. Lima 1, D.R. Barros 1, F.N. Silva 1, P. Alfenas-Zerbini 1, C.S. Rocha 1, E.W. Kitajima 2 and F.M. Zerbini 1 1 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, , Brazil; 2 NAP/MEPA, ESALQ-USP, Piracicaba, SP, , Brazil 9 Begomoviruses infecting weeds in Bolivia A.T.M. Lima 1, F.N. Silva 1, C.S. Rocha 1, T.F.S. Antunes 1, D.R. Barros 1, G.P. Rosales 2, N. Ortuño 2, A. Gandarillas 2, R.O. Resende 3 and F. M. Zerbini 1 1 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, , Brazil; 2 Fundación PROINPA, Av. Meneces s/n. Km. 4, Zona El Paso, Cochabamba, Bolivia; 3 Dep. de Biologia Celular/Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, , Brazil 10 Malvaceous plants are infected with novel Begomovirus species th International ssdna Comparative Virology Workshop

27 POSTER SESSION M.M.S. Almeida 1,2, L.V. Hoffmann 3, J.C. Barbosa 4, S.A. Aranha 1,2, and A.K. Inoue- Nagata 1,2 1 Universidade de Brasília, Brasília, Brazil; 2 Embrapa Vegetables, BR 060, Km 09, C.P. 218, , Brasília, Brazil; 3 Embrapa Algodão, GO 462, Km 12, zona rural, C.P. 179, , Santo Antônio de Goiás, Brazil; 4ESALQ/USP Departamento de Fitopatologia e Nematologia, Piracicaba, Brazil 11 Beak and feather disease virus: Global genetic diversity, geographical and host species distribution of isolates. Arvind Varsani 1,2, Guy L. Regnard 3, Inga I. Hitzeroth 3, Robert R. Bragg 4, Edward P. Rybicki 3,5 1 School of Biological Sciences, University of Canterbury, Christchurch, 8140, New Zealand, 2 Electron Microscope Unit, University of Cape Town, Rondebosch, Cape Town, 7701, South Africa, 3 Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, Cape Town, 7701, South Africa, 4 Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, 9301, South Africa, 5 Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, Cape Town, 7925, South Africa 12 Molecular characterization of two new strains of Okra yellow mosaic Mexico virus. Y. Cárdenas-Cornejo, M.Cantú-Iris, S. Ambriz-Granados, and G. Argüello-Astorga Instituto Potosino de Investigación Científica y Tecnológica, A.C. Camino a la Presa San José 2055, Colonia Lomas 4ta. Sección, San Luis Potosí, S.L.P., México C.P Genetic diversity and synergistic interaction among newly emerging begomoviruses associated with chilli leaf curl disease in India. S. Chakraborty, A. K. Singh, R. Vinothkumar, N. Khushwaha, B. Chattopadhyay Molecular Virology laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi Molecular characterization of a novel begomovirus infecting the weed Corchorus hirtus in Brazil. R. S. Fontenele 1, 2, R. Blawid 1, 2, S. G. Ribeiro 1 1 Embrapa Recursos Genéticos e Biotecnologia, Pq Estação Biológica, Brasília, Brazil, 2 Universidade de Brasília, Instituto de Biologia, Brasília, Brazil 15 Characterization of two newly discovered curtoviruses isolated from spinach in south-central Arizona. Cecilia Hernández-Zepeda 1, Gerardo Arguello-Astorga 2, and Judith K. Brown 1 6 th International Geminivirus Symposium 21

28 POSTER SESSION 1 School of Plant Sciences, The University of Arizona, 85721, Tucson, AZ USA; 2 Division de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica,, A.C. San Luis Potosí, México 16 TYLCV in Northern Mexico, an emerging viral disease of tomato crops in single or mixed infection with natives begomovirus. Méndez-Lozano J., Romero-Romero, J.L., Camacho-Beltrán E., Orduño-Vega, W., Gámez- Jiménez, C., Magallanes-Tapia, M.A., and Leyva-López, N.E. Departamento de Biotecnología Agrícola, CIIDIR Unidad Sinaloa del Instituto Politécnico Nacional. Blv. Juan de Dios Bátiz Peredes 250, C.P Guasave, Sinaloa, México 17 Veni Vidi Vici - The tale of the Cotton leaf curl Burrewala virus in India Prem Rajagopalan 1, Prashanth Katturi 1, Amruta S. Naik 1, Meera Kurulekar 1, Ravi Kankanallu 2, Radha Anandalakshmi 1 1 Plant-Virus Interactions Lab, Mahyco Research Center, Post Box no. 76, Jalna , India, 2 Vegetable Research Center, Mahyco, Bengaluru, India 18 Molecular characterization of an isolate of Bean golden mosaic virus infecting soybean in Argentina. P.E Rodríguez-Pardina 1, B. Márquez-Martín 2, I.G. Laguna 1 and J. Navas-Castillo 2 1 INTA-IFFIVE, Camino 60 Cuadras Km 5 ½, X5020ICA Córdoba, Argentina. 2 Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM-UMA-CSIC), Algarrobo- Costa, Málaga, Spain. 19 Tomato severe rugose virus and Tomato mild mosaic virus detected in weeds in natural infections in Southeastern Brazil F.N. Silva, C.S. Rocha, P. Alfenas-Zerbini, G.P. Castillo-Urquiza, A.T.M. Lima & F. M. Zerbini Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, , Brazil 20 Isolation and characterization of a pepper-infecting curtovirus from Mexico M. Taja, J.A. Mauricio-Castillo, B. Bañuelos-Hernandez and G.R. Argüello-Astorga Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), A.C. Camino a la Presa San José 2055, Colonia Lomas 4ta. Sección, San Luis Potosí, S.L.P., México C.P th International ssdna Comparative Virology Workshop

29 POSTER SESSION 21 Identification of a Begomovirus weed (Allosidastrum cf pyramidatum (Lav.) Kropov) associated with the tomato crop in Colombia J.C. Vaca-Vaca and K. Lopez-Lopez Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia. Palmira, Valle del Cauca, Colombia 22 First Identification of a Begomovirus infecting yellow passion fruit in Colombia J.C. Vaca-Vaca, J.F. Betancur-Perez, and K. Lopez-Lopez Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia. Palmira, Valle del Cauca, Colombia 23 Divergent monopartitite geminiviruses: Eragrostis curvula streak virus and Turnip curly top virus Arvind Varsani 1,2, Dionne N Shepherd 3, Kyle Dent 2,3, Aderito L Monjane 3, Edward P Rybicki 3,4, Jahangir Heydarnejad 5, Fakhrosadat Khosrowfar 6, Hossain Massumi 5, Rob W. Briddon 7, Darren P Martin 4 1 School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, New Zealand; 2 Electron Microscope Unit, University of Cape Town, Rondebosch, Cape Town,, South Africa; 3 Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, Cape Town, South Africa; 4 Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, Cape Town, South Africa; 5 Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran; 6 Plant Protection Management, Fars Province, Shiraz, Iran; 7 National Institute for Biotechnology and Genetic Engineering, Jhang Road, P.O. Box 577, Faisalabad, Pakistan 24 Characterization of a new begomovirus infecting Solanum lycopersicum in Venezuela K.A Zambrano a, J. Carrillo-Tripp b, O. Carballo a, R.F. Rivera-Bustamante b, and E. Marys a a Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Panamericana Km 11, Caracas, Venezuela. b Departamento de Ingeniería Genética, Cinvestav Irapuato, Km. 9.6, Libramiento Norte, P.O. Box 629, C.P ,Irapuato, Guanajuato, México Virus-Host Interactions 25 Four suppressors of RNA silencing are encoded by cotton leaf curl Multan begomovirus complex 6 th International Geminivirus Symposium 23

30 POSTER SESSION Imran Amin 1*, Khadim Hussain 1,2*, Rashid Akbergenov 2, Jitender S. Yadav 3, Javaria Qazi 1, Shahid Mansoor 1, Thomas Hohn 2, Claude M. Fauquet 3 and Rob W. Briddon 1 1 National Institute for Biotechnology and Genetic Engineering, Jhang Road, P.O. Box 577, Faisalabad, Pakistan, 2 Institute of Botany, University of Basel, Switzerland, 3 ILTAB/Danforth Plant Science Center, 975 N. Warson Rd, St Louis, MO 63132, USA 26 A reverse genetics approach to discover tomato genes underlying resistance to Tomato yellow leaf curl virus. Eybishtz Assaf, Sade Dagan and Czosnek Hanokh (Henryk) Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel 27 Interaction of the movement protein with a begomovirus-induced cell wall protein. K.M. Balmant 1,2, M.C.T. Leite 2, C.M. Carvalho 1,3, and E.P.B. Fontes 1,2 1 INCT em Interações Planta-Praga/Fapemig/CNPq/MCT, 2 Departamento de Bioquímica e Biologia Molecular/BIOAGRO, 3 Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brasil, Identification of cellular targets for NIG (NSP-interacting GTPase) involved in nucleocytoplasmic transport of proteins. J.P.B. Machado 1,2, I.P. Calil1,2, C.M. Carvalho 2,3, and E.P.B. Fontes 1, 2 1 Dep. Bioquímica e Biologia Molecular/BIOAGRO, 2 INCT em Interações Planta-Praga /CNPq/Fapemig/MCT, 3 Dep. Fitopatologia/BIOAGRO. Universidade Federal de Viçosa 29 APC7, a component of the Anaphase Promoting Complex, in begomovirus replication F.P. Bruckner 1, F.N. Silva 2, T.B. Carvalho 3, P.C.G. Ferreira 3, F.M. Zerbini 2, E.P.B. Fontes 4 and P. Alfenas-Zerbini 1 1 Dep. de Microbiologia/BIOAGRO, Universidade Federal de Viçosa, , Viçosa, MG, Brazil; 2 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa; 3 Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, , Rio de Janeiro, RJ, Brazil; 4 Dep. de Bioquímica e Biologia Molecular/BIOAGRO, Universidade Federal de Viçosa 30 Differentially expressed proteins in begomovirus-resistant tomato inoculated with Tomato chlorotic mottle virus L.S.T. Carmo 1,2, C. Lacorte 1, R.S. Fontenele 1,2, R.O. Resende 2, L.P. Silva 1, C. Bloch 1, S.G. Ribeiro 1, A. Mehta th International ssdna Comparative Virology Workshop

31 POSTER SESSION 1 Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília, Brasil, 2 Universidade de Brasília, Departamento de Biologia Celular, Brasília, Brasil 31 Soybean golden mosaic virus: a new soybean-infecting begomovirus in Southeast Brazil D. Côco 1,2, F.M. Zerbini 1,3, P. Alfenas-Zernini 4, A. Nagata 1,5, and E.P.B. Fontes 1,2 1 INCT em Interações Planta-Praga, Fapemig/CNPq/MCT, 2 Departamento de Bioquímica e Biologia Molecular/BIOAGRO, 3 Departamento Microbiologia/BIOAGRO, 4 Dep. Microbiologia /BIOAGRO, Univ. Federal de Viçosa, Viçosa, MG, Brasil, , 5 Embrapa Soja, Londrina, Paraná, Brasil 32 A broad approach to identify cellular proteins required for Geminivirus infection using virus induced gene silencing. T. Rosas-Díaz, R. Lozano-Durán, and E.R. Bejarano. Instituto de Hortofruticultura Subtroplical y Mediterránea La Mayora, Univ. de Málaga-Consejo Superior de Investigación Científicas (IHSM-UMA-SCIC). 33 Systemic acquired resistance (SAR) is involved in the geminivirus resistance observed in an accession of Capsicum chinense Jacq. M. A. García-Neria and R. F. Rivera-Bustamante Departamento de Ingeniería Genética, Cinvestav Irapuato, Km. 9.6 Libramiento Norte, P.O. Box 629, C.P , Irapuato, Guanajuato, México 34 Quantitative trait loci analysis and whole genome sequences as tools to decipher the resistance to Cabbage leaf curl virus in the Pla-1 ecotype of Arabidopsis thaliana. O. Guerra-Peraza and D. Robertson North Carolina State University, Raleigh NC 27695, USA 35 Analysis of DNA and small RNA accumulation during the interaction of pepper plants with different strains and chimeric viruses of Pepper golden mosaic virus Cecilia Hernandez-Zepeda, Ali M. Idris, Judith K. Brown School of Plant Sciences, The University of Arizona, 85721, Tucson, AZ. USA 36 BCTV and CaLCuV display a different interaction with the cell cycle machinery in Arabidopsis. J.T. Ascencio-Ibañez, L. Sardo, D. Vu, R. Coppersmith, G.P. Accotto and L.K. Hanley- Bowdoin 6 th International Geminivirus Symposium 25

32 POSTER SESSION Molecular and Structural Biochemistry Department, 128 Polk Hall, North Carolina State University, Raleigh NC The contribution of an alphasatellite-like molecule to symptom severity when in the presence of a begomovirus-beta satellite complex infecting tomato in Oman A.M. Idris 1,2, Cecilia Hernández-Zepeda 1, J.-K. Zhu 2, Judith K. Brown 1 1 School of Plant Sciences, The University of Arizona, 85721, Tucson, AZ. USA; 2 Plant Stress Genomics and Technology Research Center, King Abdullah University of Science and Technology, Thuwal , Kingdom of Saudi Arabia 38 Characterization of recovery phenomenon in Tomato leaf curl New Delhi virus: Correlation between viral DNA, transcripts and mirna levels Jyothsna. P and Malathi V.G. Advanced Centre for Plant Virology, Div, of Plant Pathology, Indian Agricultural Research Institute, New Delhi, INDIA 39 Molecular dissection of Ty-5, a Tomato yellow leaf curl virus resistance locus in the tomato line TY172 derived from Solanum peruvianum I. Levin, U. Karniel, I. Anbinder, M. Reuveni, S. Nahon, H. Shlomo, L. Chen, and M. Lapidot Institute of Plant Sciences, Agricultural Research Organization, the Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel 40 The rpl10 family as NIK substrates K.V.G. Lopes 1,2, A.R. Pataro 1,2, F.A. Costa 1,2, A.A. Santos 1,2, E.P.B. Fontes 1,2 1 Departamento de Bioquímica e Biologia Molecular/BIOAGRO, 2 INCT em Interações Planta- Praga/CNPq/Fapemig/MCT, Universidade Federal de Viçosa, Viçosa, MG, Brasil, Geminiviruses subvert ubiquitination and inhibit jasmonate signalling by altering CSN-mediated de-rubylation of SCF E3 ligase complexes. R. Lozano-Durán 1, T. Rosas-Díaz 1, G. Gusmaroli 2,4, A.P. Luna 1, Renau JP 3, X.W. Deng 2 and E.R. Bejarano 1* (1) Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, Universidad de Málaga- Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Dept. Biología Celular, Genética y Fisiología, Universidad de Málaga, Campus Teatinos, Málaga, Spain. (2) Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven. Connecticut , USA. (3) UMR INRA CNRS UEVE, 2 rue Gaston Crémieux, 26 4 th International ssdna Comparative Virology Workshop

33 POSTER SESSION CP 5708, Evry, France. (4) Current Department of Science and Mathematics, University of South Carolina Beaufort, Bluffton, SC USA 42 Functional diversity of gene silencing suppressors from Tomato yellow leaf curl disease viruses. A.P. Luna 1, G. Morilla 2, O. Voinnet 2 and E.R. Bejarano 1* (1) Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Dept. Biología Celular, Genética y Fisiología, Universidad de Málaga, Campus Teatinos, Málaga, Spain. (2) Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, Strasbourg Cedex, France 43 Genetic silencing of cytoplasmic thioredoxins based on VIGS vector in pepper plants (Capsicum annuum L (cv Anaheim) infected with PepGMV. Luna-Rivero Marianne 1, Villanueva-Alonzo Hernan 1, Cecilia Hernández-Zepeda 2 y Moreno-Valenzuela Oscar A. 1 1 Unidad de Bioquímica y Bilogía Molecular de Plantas, Centro de Investigación Científica de Yucatán. Calle 43 No. 130, Colonia Chuburná de Hidalgo, Mérida, Yucatán, México. 2 School of Plant Sciences, The University of Arizona, Tucson, AZ 85721, USA 44 Development of vectors-mediated expression of dsrna for PTGS activation in Lycopersicum esculentum D. Medina-Hernandez 1, F. Tenllado-Perlalo 2, CE. Angulo-Valadez 1, and R.J. Holguin-Peña 1 1 Biología Molecular de Plantas, CIBNOR, Mar Bermejo No. 195, Col. Playa Palo de Santa Rita Apdo. Postal 128; La Paz, BCS 23090, México. 2 Biología Medioambiental, CIB; Ramiro de Maeztu 9, Madrid. 45 Silencing CchGLP gene increase symptoms severity by geminivirus infections in Capsicum chinense BG-3821 L. Mejia-Teniente 1, I. Torres-Pacheco 1, C.I. Munoz-Sanchez 2, L. Guevara-Olvera 2, G. Acosta-García 2, M.M. González-Chavira 3 R. F. Rivera-Bustamante 4, and R.G. Gonzalez- Guevara 1 1 Cerro Universitario Cerro de las Campanas, s/n, Col. Las Campanas. C.P Santiago de Querétaro, Querétaro, México. 2 Depto. Ingeniería Bioquímica. Instituto Tecnológico de Celaya. Celaya, Gto, México. 3 Unidad de Biotecnología del Bajío, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias-Campo Experimental Bajío. Celaya, Gto, México. 4 CINVESTAV- Irapuato, Carretera Irapuato-León Km 9.6; Irapuato, Gto, México. 6 th International Geminivirus Symposium 27

34 POSTER SESSION 46 Deep sequencing analysis of virus-derived small RNAs in tomato plants infected by Tomato yellow leaf curl Sardinia virus L. Miozzi, V. Pantaleo, E. Noris, J. Burgyan, G.P. Accotto Istituto di Virologia Vegetale C.N.R., Strada delle Cacce 73, Torino, Italy 47 Tomato leaf curl virus or Tomato leaf curl New Delhi virus DNA A and Cotton leaf curl disease betasatellite can cause mild transient symptoms in cotton. Muhammad Saeed National Institute for Biotechnology and Genetic Engineering, PO Box 577, Jhang Road, Faisalabad, Pakistan 48 Altered Gene Expression Profile of Resistant Chilli Genotype Infected With Chilli Leaf Curl Virus Nirbhay Kushwaha 1, Pranab Sahu 2, Manoj Prasad 2 and Supriya Chakraborty 1 1 Molecular Virology laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi , 2 National Institute of Plant Genome Research, New Delhi Tomato cultivar tolerant to Tomato leaf curl New Delhi virus infection induces virusspecific sirna accumulation and defense associated host gene expression. P. P. Sahu 1*, S. Chakraborty 2, M. Singh 3, D. Chattopadhyay 1, and M. Prasad 1* 1 National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi , INDIA, 2 School of Life Sciences, Jawaharlal Nehru University, New Delhi , INDIA, 3 Indian Institute of Vegetable Research, Gandhinagar, Varanasi , INDIA 50 Natural resistance against Mungbean yellow mosaic India virus correlates with early viral transcript degradation and differential sirna production in Glycine max. Rajiv Kumar Yadav and Debasis Chattpoadhyay Lab 103, National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi , India 51 Global transcriptome profile of Arabidopsis thaliana infected with South African cassava mosaic virus M.E.C. Rey, E. Pierce and F. van Schalk School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, South Africa, th International ssdna Comparative Virology Workshop

35 POSTER SESSION 52 Transcriptomic and proteomic analysis of Maize infected with Maize streak virus. Rizwan Ali Syed 1,3, Zac Mcdonald 2,3, Dionne N. Shepherd 1, Darren P. Martin 2, Suhail Rafudeen 1, Edward P. Rybicki 1 1 Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, Cape Town, South Africa, 2 Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, Cape Town, South Africa, 3 These authors are co-first authors 53 The Tomato yellow leaf curl virus, Sardinian isolate (TYLCSV) C4 protein is a putative transcriptional gene silencing (TGS) suppressor. E.A. Rodriguez-Negrete, R. Lozano-Duran, A.G. Castillo, and E.R. Bejarano Departamento de Genética. Facultad de Biología. Campus de Teatinos. IHSM, UMA-CSIC Málaga, Spain 54 Interaction between Geminivirus Replication Protein and the SUMO Conjugating Enzyme Is Required for Viral Infection. Miguel A. Sánchez-Durán 1, Mary B. Dallas 2, José T. Ascencio-ibañez 2, Javier Ruiz-Albert 1, Linda Hanley-Bowdoin 2 and Eduardo R. Bejarano 1 55 The infective cycle of Cabbage leaf curl virus (CaLCuV) is affected by CRUMPLED LEAF (CRL) gene in Arabidopsis thaliana. D.L. Trejo-Saavedra, J.P. Vielle-Calzada and R.F. Rivera-Bustamante Departamento de Ingeniería Genética, Cinvestav Irapuato, Km. 9.6 Libramiento Norte, P.O. Box 629, C.P , Irapuato, Guanajuato, México. 56 Arabidopsis synaptotagmins: their roles in virus cell-to-cell movement and plant development. Asako Uchiyama, Harumi Shimada-Beltran, Sondra G. Lazarowitz Cornell University, Dept. of Plant Pathology and Plant-Microbe Biology, Ithaca, NY USA 57 Gene expression profile of Nicotiana benthamiana infected with South African cassava mosaic virus using a cross-species cdna microarray approach. F. Van Schalk and M.E.C. Rey School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, South Africa, th International Geminivirus Symposium 29

36 POSTER SESSION 58 Virus-induced gene silencing (VIGS) in plants of commercial and scientific interest. Villanueva-Alonzo, H. 1, Guerra-Peraza, O. 2, Robertson, D. 2, López-Ochoa, L. 1, and Moreno-Valenzuela O 1 1 Unidad de Bioquímica y Bilogía Molecular de Plantas, Centro de Investigación Científica de Yucatán. Calle 43 No. 130, Colonia Chuburná de Hidalgo, Mérida, Yucatán, México, 2 North Carolina State University, Raleigh, NC Functional analysis of the NIK-mediated antiviral signaling pathway in tomato plants. A.A. Santos 1, J.A. Condori-Apfata 2, O.B. Brustolini 2, F.M. Zerbini, 3 E.P.B. Fontes 2 1 INCT em Interações Planta-Praga/Fapemig/CNPq/MCT, 2 Departamento de Bioquímica e Biologia, Molecular/BIOAGRO, 3 Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brasil, Downstream components of NIK-mediated antiviral signaling. C. Zorzatto, J.P.B. Machado, K.V.G. Lopes, A.A. Santos and E.P.B. Fontes INCT em Interações Planta-Praga/CNPq/Fapemig/MCT, Departamento de Bioquímica e Biologia Molecular, BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brazil Epidemiology/Transmission 61 Distribution of Begomovirus associated with tomatoes in the Sultanate of Oman. A.J. Khan, A.O. AL-Matrushi and A. Al-Shihi. Department of Crop Sciences, College of Agricultural & Marine Sciences, Sultan Qaboos University, P.O. Box-34, Al-Khod 123, Sultanate of Oman 62 Distribution and molecular variability of begomovirus affecting the main crops in Cuba: Epidemiological elements. Y. Martínez-Zubiaur; M. Quiñonez, E. Fiallo-Olive, Y. Marrero, M. de los A. Martínez, I. Palenzuela, I. Miranda Departamento de Fitopatología, Centro Nacional de Sanidad Agropecuaria. Apdo 10, San José de las Lajas. Habana, Cuba 63 Multiplex PCR for the simultaneous detection of DNA (geminivirus) and RNA (potyvirus) viruses infecting cassava. Maruthi MN 1, Abarshi MM 1, Mohammed IU 1, Kumar L 2, and Legg JP th International ssdna Comparative Virology Workshop

37 POSTER SESSION 1 Natural Resources Institute, University of Greenwich, Chatham Maritime, Kent ME4 4TB, United Kingdom, 2 Internatioanl Institute for Tropical Agriculture (IITA), Ibadan, Nigeria 64 Studies on single and double infection of bipartite begomoviruses by whitefly transmission. M.A. Macedo 1,2, V.N. Guimarães 1,2, T.G. Pereira 1,2, P.P.F. Lemos 1,2, A.K. Inoue-Nagata 1,2. 1 Universidade de Brasília, Brasília, Brazil; 2 Embrapa Vegetables, Brasília/Anápolis, BR 060 Km 09, C.P. 218, , Brasília, Brazil 65 Epidemiology and eco-friendly management of yellow mosaic virus of grain legumes in India. YPS Rathi B-55, Pallavpuram - I, Meerut - Meerut , UP, India. 66 Infectivity of blackgram isolate of Mungbean yellow mosaic India virus on cowpea. Rouhibakhsh 1, A. and V. G. Malathi 2 Department of Horticulture, Agricultural Faculty, Ilam University, Ilam, Iran. Advance Center for Plant Virology, Division of Plant Pathology, IARI, New Delhi, , India. 67 Study of weeds as begomovirus source to tomato plants in Brazil. S.S. Barreto 1, M.A. Reis 2, P. P. F. Lemos 1 and A. K. Inoue-Nagata 1,2 1 Universidade de Brasília, Brasília, Brazil; 2 Embrapa Vegetables, BR 060 Km 09, C.P. 218, , Brasília, Brazil 68 Molecular characteristics of Cotton leaf curl Multan virus and its associated satellite DNA infecting Hibiscus rosa-sinensis and okra in China. Zifu He 1, 2, Dong Di 1, Mingjie Mao 1, Xiao-man She 1, Judith K Brown 2 1 Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, , China; 2 School of Plant Sciences, The University of Arizona, Tucson, AZ, 85721, USA. Replication/Gene Expression 69 Two strains of Euphorbia mosaic virus are incompatible in replication and contain short Rep sequences in the BV1 promoter region. B. Bañuelos-Hernández, J. Gregorio-Jorge, G.R. Argüello-Astorga 6 th International Geminivirus Symposium 31

38 POSTER SESSION Instituto Potosino de Investigación Científica y Tecnológica, A.C. Camino a la Presa San José 2055, Colonia Lomas 4ta. Sección, San Luis Potosí, S.L.P., México C.P The impact of geminiviral proteins on the nuclear architecture Krenz B. 1, Neugart F. 2, Jeske H. 1 and Kleinow T 1 1 University of Stuttgart, Institute of Biology, Dpt. of Molecular Biology and Plant Virology, Stuttgart, Germany, 2 University of Stuttgart, Institute of Cell Biology, Stuttgart, Germany 71 Visualization of Abutilon mosaic virus replication-associated protein self-interaction by bimolecular fluorescence complementation in planta Krenz B. 1, Neugart F. 2, Kleinow T. 1 and Jeske H. 1 1 University of Stuttgart, Institute of Biology, Dpt. of Molecular Biology and Plant Virology, Stuttgart, Germany, 2 University of Stuttgart, Institute of Cell Biology, Stuttgart, Germany 72 The begomoviral conserved late element (CLE) is a functional target of plant transcription factors M. Cantú-Iris 1, A. Juárez-Reyes 1, R. Rivera-Bustamante 2, and G.R. Argüello-Astorga 1 1 Instituto Potosino de Investigación Científica y Tecnológica, A.C. Camino a la Presa San José 2055, Colonia Lomas 4ta. Sección, San Luis Potosí, S.L.P., México C.P Departamento de Ingeniería Genética, Cinvestav Irapuato, P.O. Box 629, C.P , Irapuato, Guanajuato, México. mariana.cantu@ipicyt.edu.mx 73 Analysis of trans-replication of a betasatellite by a begomovirus Khadim Hussain, Mazhar Hussain, Shahid Mansoor and Rob W. Briddon National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, P.O. Box 577. Faisalabad Pakistan 74 Characterization of strong promoters from American begomoviruses A. Londoño, L.R. Riego-Ruiz, G.R. Arguello-Astorga División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica. Camino a la Presa San José 2055, Col. Lomas 4 sección, P.O. Box 78216, San Luis Potosí, SLP, México 32 4 th International ssdna Comparative Virology Workshop

39 POSTER SESSION Genetic Diversity/Evolution 75 Genetic diversity by extensive recombination in the complex of monopartite begomoviruses infecting sweet potato in Brazil L.C. Albuquerque 1,2,4, B. Pinheiro 1,2, S.G. Ribeiro 3, R.O. Resende 2, E. Moriones 4, A.K. Inoue-Nagata 1,2, and J. Navas-Castillo 4 1 Embrapa Vegetables, C.P. 218, Brasília, DF, , Brazil. 2 Universidade de Brasília, Brasília, DF, , Brazil. 3 Embrapa Cenargen, C.P , Brasília, DF, CEP , Brazil. 4 Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM- UMA-CSIC), Algarrobo-Costa, Málaga, Spain. 76 The first monopartite begomoviruses discovered in Western Hemisphere endemic eudicots: evidence for swepovirus diversification in wild and cultivated hosts J. K. Brown 1, H. Delatte 2, J. Bird, 3 and Ali M. Idris 1,4 1 School of Plant Sciences, The University of Arizona, 85721, Tucson, AZ, USA; 2 CIRAD, UMR PVBMT, Pôle de Protection des Plantes, 7 chemin de l IRAT, Saint Pierre, La Réunion, France; 3 College of Agricultural Sciences, University of Puerto Rico, Rio Piedras, PR 00928; 4 Plant Stress Genomics and Technology Research Center, King Abdullah University of Science and Technology, Thuwal , Kingdom of Saudi Arabia 77 Genetic structure of tomato-infecting begomovirus populations in two growing regions of Southeastern Brazil G.P. Castillo-Urquiza 1, P. Alfenas-Zerbini 1, J.E.A. Beserra-Junior 1, E.S.G. Mizubuti 1, A. Varsani 2, D.P. Martin 3 and F.M. Zerbini 1 1 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, , Viçosa, MG, Brazil; 2 School of Biological Sciences, University of Canterbury, Private bag, Christchurch, 8140, New Zealand; 3 Computational Biology Group, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa 78 Epidemiology and molecular characterization of Tomato curly stunt virus and its insect vector Bemisia tabaci in South Africa L.L. Esterhuizen 1, S.W. Van Heerden 2, M.E.C. Rey 3 and H. Van Heerden 4 1 Department of Biochemistry, University of Johannesburg, Johannesburg, South Africa, 2 Sakata Vegenetics RSA (Pty) Ltd., Lanseria, South Africa, 3 Department of Cell and Molecular Biochemistry, University of Witwatersrand, Johannesburg, South Africa, 4 Department of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South Africa 6 th International Geminivirus Symposium 33

40 POSTER SESSION 79 Bipartite and monopartite begomoviruses infecting Ipomoea spp. and Merremia spp. in Venezuela E. Fiallo-Olivé 1,2, B. Márquez-Martín 1, F. Geraud-Pouey 3, D. T. Chirinos 3, J. Navas- Castillo 1 and E. Moriones 1 1 Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, Universidad de Málaga- Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Algarrobo-Costa, Málaga, Spain. 2 Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba. 3 Universidad del Zulia, Maracaibo, Zulia, Venezuela. 80 Begomovirus diversity and recombination in the Caribbean, a hot melting pot in the New World. E. Fiallo-Olivé 1,2, E. Moriones 2, J. Navas-Castillo 2 and Y. Martínez-Zubiaur 1 1 Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba. 2 Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental La Mayora, Algarrobo-Costa, Málaga, Spain 81 Diversity and evolutionary dynamics of begomovirus infecting a wild crop relative, Capsicum annuum var. glabriusculum P. González-Jara 1, M. Rodelo-Urrego 1, A. Fraile 1, D. Piñero 2 and F. García-Arenal 1 1 Centro de Biotecnología y Genómica de Plantas, UPM-INIA, Universidad Politécnica de Madrid. Campus de Montegancedo, Pozuelo de Alarcón, Spain. 2 Instituto de Ecología, Universidad Nacional Autónoma de México, AP , México, D.F, Mexico. 82 Molecular characterization of Sweet potato leaf curl virus (SPLCV) Korea isolates Jungan Park 1, Hyejung Lee 1, Yuchul Jung 1, Jaelim Yu 1, Hong-Soo Choi 2, and Sukchan Lee 1 1 Department of Genetic Engineering, Sungkyunkwan University, Korea, 2 Agricultural Microbiology Division, National Academy of Agricultural Science, Korea 83 Agroinoculation of cloned Honeysuckle yellow vein virus (HYVV) isolated from Lonicera japonica and its recombination study Hyejung Lee, Gunsup Lee, Jungan Park, Seungchan Cho, Jisook Chae, Eunbyul Ji, Kangsan Roh, Taejune Jun, Sukchan Lee Department of Genetic Engineering, Sungkyunkwan University, Korea 34 4 th International ssdna Comparative Virology Workshop

41 POSTER SESSION 84 Investigations towards understanding the evolution and global movement of Abutilon mosaic virus Simona Kraberger 1, Daisy Stainton 1, Karyna Rosario 2, Holger Jeske 3, Darren P Martin 4, David Collings 1 and Arvind Varsani 1, 5 1 School of Biological Sciences, University of Canterbury, Christchurch, New Zealand; 2 College of Marine Science, University of South Florida, St Petersburg, Florida; 3 Department of Molecular Biology and Plant Virology, Institute of Biology, University of Stuttgart, Stuttgart, Germany; 4 Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; 5 Electron Microscope Unit, University of Cape Town, Cape Town, South Africa 85 Discovering circomics! Deep insights into plant viral circular genomes Björn Krenz 1, Tobias Paprotka 2, Judith Horn 1, Benjamin Schäfer 1, Patricia Wyant 1, Stephan Strohmeier 1 and Holger Jeske 1 1 University of Stuttgart, Institute of Biology, Dpt. of Molecular Biology and Plant Virology, Stuttgart, Germany, 2 Viral Mutation Section, HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702, USA. 86 Phylogenetic analysis of TbLCV and its evidence for recombination with TYLCV Hyejung Lee 1, Jungan Park 1, Eui-Joon Kil¹,Mangyong Jung 1,Jin-gweonYang¹, Hong-Soo Choi 2, and Sukchan Lee 1 * 1 Department of Genetic Engineering, Sungkyunkwan University, Korea, 2 Agricultural Microbiology Division, National Academy of Agricultural Science, Korea 87 Phylogeny and recombination analysis of Tomato yellow leaf curl virus (TYLCV) and Bemisia tabaci in Korea Hyejung Lee¹, Jungan Park¹, Eui-Joon Kil¹, Jin-gweon Yang¹, Kyeong-yeoll Lee², Hong-Soo Choi³, Sukchan Lee¹ ¹Department of Genetic Engineering, Sungkyunkwan University, Korea, 2 School of Applied Biosciences, Kyungpook National University, Korea, ³Agricultural Microbiology Division, National Academy of Agricultural Science, Korea 88 Diversity of tomato begomoviruses in the three major tomato growing states of Brazil A.T.M. Lima 1, F.Y.B. Naito 2, F.N. Silva 1, C.S. Rocha 1, D.R. Barros 1, G.P.C. Urquiza 1, A.W.O. Malta 1, M.F. Basso 1, M.T. Godinho 1, E.S.G. Mizubuti 1, F.M. Zerbini 1 and A.K. Inoue-Nagata 2,3 1 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, , Brazil; 2 Dep. de Fitopatologia/Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, , Brazil; 3 Embrapa Hortaliças, BR 060 Km 09, C.P. 218, Brasília, DF, , Brazil 6 th International Geminivirus Symposium 35

42 POSTER SESSION 89 Begomoviruses infecting weeds in Bolivia A.T.M. Lima 1, F.N. Silva 1, C.S. Rocha 1, T.F.S. Antunes 1, D.R. Barros 1, G.P. Rosales 2, N. Ortuño 2, A. Gandarillas 2, R.O. Resende 3 and F. M. Zerbini 1 1 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, , Brazil; 2 Fundación PROINPA, Av. Meneces s/n. Km. 4, Zona El Paso, Cochabamba, Bolivia; 3 Dep. de Biologia Celular/Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, , Brazil. 90 Genetic analysis and phylogeographic history of MSV reveals the spatial and temporal origins of contemporary MSV-A recombinant lineages and identifies southern Africa as the ultimate origin of Maize streak disease A. L. Monjane, G. W. Harkins, D. P. Martin, P. Lemey, D. N. Shepherd, P. Lefeuvre, E. P. Rybicki, B. E. Owor, R.S. Ali, B. Flett, M. Ramusi, S. Oluwafemi, and A. Varsani Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Observatory, Cape Town, 7925, South Africa 91 Diversity of Dicot-infecting Mastreviruses Huma Mumtaz 1, Safaa G. Kumari 2, Nazia Nahid 1, Darren P. Martin 3, Shahid Mansoor 1 and Rob W.Briddon 1 1 Agriculture Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan, 2 Virology Laboratory, International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria, 3 Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa 92 Genetic diversity of DNA satellites associated with Ipomoea-infecting monopartite begomoviruses H.P. Trenado 1, E. Fiallo-Olivé 1,2, G. Lozano 1, D. Chirinos 3, F. Geraud-Pouey 3, E. Moriones 1, R.W. Briddon 4, and J. Navas-Castillo 1 1 Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, Universidad de Málaga- Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Algarrobo-Costa, Málaga, Spain. 2 Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba. 3 Universidad del Zulia, Maracaibo, Zulia, Venezuela. 4 Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan 36 4 th International ssdna Comparative Virology Workshop

43 POSTER SESSION 93 Begomovirus species diversity in South American weeds T.G. Pereira 1,2, F.Y.B. Naito 1,2, R.S. Fontenele 1,3, R. Blawid 1,3, F.R. Fernandes 4, A.T.M. Lima 4, F.N. Silva 4, C.S. Rocha 4, G.P. Rosales 5, C.L. Oliveira 1,2, R.O. Resende 1, F.M. Zerbini 4, S.G. Ribeiro 3, F. J. L. Aragão 3, A.K. Inoue-Nagata 1, 2 1 Universidade de Brasília,CEP Brasília, Brazil. 2 Embrapa Vegetables, BR 060 Km 09, C.P. 218, CEP , Brasília, Brazil. 3 Embrapa Recursos Genéticos e Biotecnologia, Pq Estação Biológica, Brasília, Brazil. 4 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, , Brazil. 5 Fundación PROINPA, Av. Meneces s/n. Km. 4, Zona El Paso, Cochabamba, Bolivia 94 Begomovirus occurring on tomatoes in Brazil and their diversity in Goiás state B. Pinheiro 1,2, L. C. Albuquerque 1,2, D. P. Martin 3, A. Varsani 3, and Inoue-Nagata A.K. 1,2 1 Universidade de Brasília, , Brasília, Brazil. 2 Embrapa Vegetables, BR 060 Km 09, C.P. 218, , Brasília, Brazil. 3 University of Cape Town, Rondebosch, Cape Town, 7701, South Africa 95 Genetic structure of a population of the begomovirus Bean golden mosaic virus (BGMV) that infects lima bean (Phaseolus lunatus L.) in the state of Alagoas, Brazil R. Ramos-Sobrinho 1,2, S.J.C. Silva 1,3, T.A.L. Silva 4, S.G. Ribeiro 4, G.S.A. Lima 2, I.P. Assunção 2 and F.M. Zerbini 1 1 Dep. de Fitopatologia/BIOAGRO, UFV, Viçosa, MG, , Brazil; 2 Dep. de Fitopatologia, CECA, UFAL, Maceió, AL, , Brazil; 3 Dep. de Fitopatologia, UFRPE, Recife, PE, , Brazil; 4 Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, , Brazil 96 Recombination and pseudorecombination driving the evolution of Tomato severe rugose virus and Tomato rugose mosaic virus: Two distinct DNA-As sharing the same DNA-B. F.N. Silva 1, A.T.M. Lima 1, C.S. Rocha 1, M. Alves-Júnior, M. 1 Hallwass 2, A.K. Inoue- Nagata 3 and F. M. Zerbini 1 1 Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, , Brazil; 2 Dep. de Biologia Celular/Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, , Brazil; 3 Embrapa Hortaliças, BR 060 Km 09, C.P. 218, Brasília, DF, , Brazil 97 Low diversity of Begomovirus species infecting Cleome affinis in Northeastern Brazil S.J.C. Silva 1,2, G.P. Castillo-Urquiza 1, G. Pio-Ribeiro 2, I.P. Assunção 3, G.S.A. Lima 3, F.M. Zerbini 1 6 th International Geminivirus Symposium 37

44 POSTER SESSION 1 Dep. de Fitopatologia/BIOAGRO, UFV, Viçosa, MG, , Brazil; 2 Dep. de Fitopatologia, UFRPE, Recife, PE, , Brazil; 3 Dep. de Fitopatologia, CECA, UFAL, Maceió, AL, , Brazil. 98 High diversity of Begomovirus species infecting Macroptilium spp. in Northeastern Brazil S.J.C. Silva 1,2, G.P. Castillo-Urquiza 1, G. Pio-Ribeiro 2, I.P. Assunção 3, G.S.A. Lima 3, F.M. Zerbini 1 1 Dep. de Fitopatologia/BIOAGRO, UFV, Viçosa, MG, , Brazil; 2 Dep. de Fitopatologia, UFRPE, Recife, PE, , Brazil; 3 Dep. de Fitopatologia, CECA, UFAL, Maceió, AL, , Brazil 99 The perspective for the use of genetically modified common beans in Brazil based on genetic diversity of Bean golden mosaic virus T. A. L. Silva, R. S. Fontenele, J. C. Faria, F. J. L. Aragão, and S. G. Ribeiro Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica Brasília, DF, Brazil 100 Genetic Diversity of Banana bunchy top virus (BBTV) in the South Pacific Tongan Archipelago. Daisy Stainton 1, Simona Kraberger 1, Karyna Rosario 2, Milen Marinov 1, Elizabeth Wiltshire 1, Matthew Walters 1, Samieluela Lolohea 3, Mana ia Halafihi 4, Ika Katoa 4, Waikato Aholelei 4 Luseane Taufa 4, David Collings 1, Darren P Martin 5 and Arvind Varsani 1, 6. 1 School of Biological Sciences, University of Canterbury, Christchurch, New Zealand; 2 College of Marine Science, University of South Florida, St Petersburg, Florida; 3 Tonga College, Tonga tapu, Kingdom of Tonga; 4 Ministry of Agriculture and Food, Forests and Fisheries, Kingdom of Tonga; 5 Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa; 6 Electron Microscope Unit, University of Cape Town, Cape Town, South Africa 101 Genetic analysis and phylogeographic history of MSV reveals the spatial and temporal origins of contemporary MSV-A recombinant lineages and identifies southern Africa as the ultimate origin of Maize streak disease A. L. Monjane, G. W. Harkins, D. P. Martin, P. Lemey, D. N. Shepherd, P. Lefeuvre, E. P. Rybicki, B. E. Owor, R.S. Ali, B. Flett, M. Ramusi, S. Oluwafemi, and A. Varsani Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Observatory, Cape Town, 7925, South Africa 38 4 th International ssdna Comparative Virology Workshop

45 POSTER SESSION Gene Expression 102 The V2 protein of Monopartite begomovirus determines virulence, hypersensitive response and suppression of posttranscriptional gene silencing. Pradeep Sharma, and Masato Ikegami* Division of Crop Improvement, Directorate of Wheat Research, Karnal , India *Graduate School of Agricultural Sciences, Tohoku University, Sendai , Japan Virus induced gene silencing 103 Silencing of PPR gene in garlic (Allium sativum L) blanco perla resistant to white rot using V-VIGS derived of PHYVV. L. Guevara-Olvera, G. Acosta-García, F. Delgadillo-Sanchez, R. F. Rivera-Bustamante and L. Chairez-Rodríguez. Departamento de Ingeniería Bioquímica, Instituto Tecnológico de Celaya, Av. Tecnológico y A. García Cubas s/n, C.P , Celaya, Guanajuato, México. Departamento de Ingeniería Genética, Cinvestav Irapuato, Km. 9.6 Libramiento Norte, P.O. Box 629, C.P , Irapuato, Guanajuato, México. INIFAP, Campo experimental Bajío, km 6 Carr. Celaya-San Miguel de Allende, Apdo. Postal 112, Celaya, Guanajuato CP Development of an efficient system for assessing gene function in the cotton plant using virus-induced gene silencing (VIGS) Cecilia Hernandez-Zepeda, ZiFu He, Judith K. Brown School of Plant Sciences, The University of Arizona, 85721, Tucson, AZ. USA Management/Control 105 RNAi-resistance to bipartite Begomoviruses in genetically modified tomatoes L.M. Almeida, T.M. Cipriano, K. Bonfim, E.O.P.L. Nogueira, S. G. Ribeiro, F. J.F.L. Aragão Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, , Brasília, DF, Brasil 6 th International Geminivirus Symposium 39

46 POSTER SESSION 106 Broad-spectrum resistance to Brazilian bipartite Begomovirus species in tomato lines carrying the Ty-1 or tcm-1 loci R. Blawid 1,2, R.F. Fontenele 1, C. Lacorte 1, R.C.P. Carvalho 2, R.O. Resende 2, L.S. Boiteux 3, M.E.N. Fonseca 3, A. C. M. Brasileiro 1, and S.G. Ribeiro 1 1 Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica - Brasília-DF, Brazil; 2 Departamento de Biologia Celular, UNB, Brasília-DF, Brazil; 3 Embrapa Hortaliças, Rodovia Brasília/Anápolis BR 060 Km 09, Gama-DF, Brazil 107 The biosafety of sirna transgenic common beans resistant to Bean golden mosaic virus. J. C. Faria 1, F. J. L. Aragão 2 1 Embrapa Arroz e Feijão, Caixa Postal 179, Santo Antônio de Goiás, GO CEP Brasil; 2 Embrapa Recursos Genéticos e Biotecnologia, Caixa Postal ----, Brasília, DF CEP - Brasil 108 Segregation pattern of genetic resistance to Tomato yellow leaf curl virus Israel (TYLCV-IL) in F 2:3 families derived from the tomato hybrid TyKing R.C. Pereira-Carvalho 1,2,3, L.S. Boiteux 1, M.E.N. Fonseca 1, J.A. Díaz-Pendón 3, E. Moriones 3, R. Fernández-Muñoz 3, AND R.O. Resende 2 1 National Center for Vegetable Crops Research (CNPH), Embrapa Vegetable Crops, Brasilia-DF, Brazil; 2 Departments of Plant Pathology and Cell Biology, UnB, Brasilia-DF, Brazil; 3 Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM-UMA-CSIC), Algarrobo- Costa, Málaga, Spain 109 Sustainability of Indian agriculture sans pesticides YPS Rathi B-55, Pallavpuram - I, Meerut - Meerut , UP, India. Mixed Infection 110 Association of two begomoviruses with severe golden mosaic disease of cowpea Naimuddin and Mohammad Akram Division of Crop Protection, Indian Institute of Pulses Research, Kanpur , India 40 4 th International ssdna Comparative Virology Workshop

47 POSTER SESSION Etiology 111 Is tomato leaf deformation disease caused by the DNA-A component of a novel begomovirus from Peru? B. Márquez-Martín 1, L. Aragón-Caballero 2, E. Fiallo-Olivé 1,3, J. Navas-Castillo 1, and E. Moriones 1 1 Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, Universidad de Málaga- Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental La Mayora, Algarrobo-Costa, Málaga, Spain; 2 Facultad de Agronomía, Universidad Nacional Agraria La Molina, La Molina, Lima, Perú; 3 Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba. 112 The first infectious clone of a swepovirus H.P. Trenado, A.F. Orílio, B. Márquez-Martín, E. Moriones, and J. Navas-Castillo Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, Universidad de Málaga- Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental La Mayora, Algarrobo-Costa, Málaga, Spain. Survey 113 Survey on begomoviruses on cucurbit species in Brazil M.F. Lima and A.K. Inoue-Nagata Embrapa Vegetables, BR 060 Km 09, Caixa Postal 218 CEP , Brasília, Distrito Federal, Brazil Other 114 Use of Virus-Induced Gene Silencing (VIGS) to accelerate genetic gain. Alvarez-Venegas, R., Zhang, Y., Kraling, K., and Tulsieram, L. Departamento de Ingeniería Genética, Cinvestav Irapuato, Km. 9.6 Libramiento Norte, P.O. Box 629, C.P , Irapuato, Guanajuato, México. 115 Analysis of geminivirus literature in the last decade. Favorite journals, most prolific authors, geographic distribution. R. Fajardo, M.A. Garcia-Neria, D.L. Trejo-Saavedra, R.F. Rivera-Bustamante Departamento de Ingeniería Genética, Cinvestav Irapuato, Km. 9.6 Libramiento Norte, P.O. Box 629, C.P , Irapuato, Guanajuato, México. 6 th International Geminivirus Symposium 41

48 POSTER SESSION 116 The spread of Tomato yellow leaf curl virus (TYLCV) from the Middle East to the world Pierre Lefeuvre, Gordon Harkins, Darren P. Martin, Philippe Lemey, Alistair J. A. Gray, Sandra Meredith, Francisco Lakay, Dionne N. Shepherd, Hossain Massumi, Jahangir Heydarnejad, and Arvind Varsani 117 Use of rolling circle amplification to enable rapid completion of Koch s postulates for circular DNA viruses. M. Lapidot 1, J.E. Polston 2, D. Guenoune-Gelbart 1, T. Sufrin-Ringwald 1, H. Capobianco 2, V. Gaba 3 Departments of 1 Vegetable Research and 3 Plant Pathology, Volcani Center, ARO, P.O.Box 6, Bet Dagan 50250, Israel, 2 Department of Plant Pathology, University of Florida, Gainesville, FL 32611, U.S.A. 118 Characterization of a New Virus Discovered by Vector-Enabled Metagenomics on the Whitefly Vector J. E. Polston 1, T. F.F. Ng 2, V. Campoverde 1, H. Capobianco 1, T. Xia 1, M. Breitbart 2 1 Dept of Plant Pathology, University of Florida, Gainesville, FL 32611, and 2 College of Marine Science, University of South Florida, th Avenue South, Saint Petersburg, Florida, USA 119 Evidence of Begomovirus mixed infections affecting tomato (Solanum lycopersicum) plants in Colombia J. C. Vaca-Vaca, J. F. Betancur-Perez, and K. Lopez-Lopez. Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia. Palmira, Valle del Cauca, Colombia. PARVOVIRIDAE Epidemiology/transmission 120 Human parvovirus 4 (PARV4): lessons from epidemiologic studies performed in south-eastern France. M. Touinssi, P. De Micco, and P. Biagini UMR CNRS 6578 Equipe «Emergence et co-évolution virale», Etablissement Français du Sang Alpes-Méditerranée et Université de la Méditerranée, 27 Bd. Jean Moulin, Marseille, France th International ssdna Comparative Virology Workshop

49 AUTHOR INDEX