Alfredo Martínez Jiménez

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Alejandra Lozano Caballero
hace 8 años
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1 Microalgal biomass cultivation in non coastal regions for biofuel production Producción de biomasa microalgal para biocombustibles en regiones no costeras a l Alfredo Martínez Jiménez Adriana Garibay Hernández M en C. Daniela Morales Sánchez Dpto. Ingeniería Celular y Biocatálisis Instituto de Biotecnología UNAM alfredo@ibt.unam.mx ASA México, D.F. Feb. 11, 2010 Instituto de Biotecnología - UNAM 1

Hernández M en C. Daniela Morales Sánchez Dpto.

2 Biofuels CO 2 Algas y a l Combustibles Fósiles Y Biocombustibles Actuales 1st Generation Almidón Sacarosa Bio-Etanol Fósiles Aceites de Petróleo Plantas Gasolina Bio-Diesel Diesel Biomasa Bio-Etanol Bio-Butanol Oleaginosas no comestibles Biodiesel 2nd Generation Cianobacterias H 2 Fotobiológico Bio-Diesel Bio-Petróleo Bio-Turbosina i 3rd Generation Future Biofuels Medium and Long Term Instituto de Biotecnología - UNAM 2

Oleaginosas no comestibles Biodiesel 2nd Generation Cianobacterias H 2 Fotobiológico Bio-Diesel

3 Global Irradiance

4 Biomass from micro-algae for biofuels Algae + Sun + CO 2 + Minerals Cell Mass (Proteins + Carbohydrates + Lipids) Instituto de Biotecnología - UNAM 4

5 N. oleoabundans a l Microalge: eukaryote Isolated from arid soil Saudi Arabia Chantanachat & Bold (1962). Oleaginoous (1970). Lipid content: 25% -??%. Triglycerides: gy main components from the lipid fraction Lipids are similar to those found in oleaginous plants Growth very well in water amended with salts, tolerates some salinity Is N. oleoabundas an adequate Microalge for biofuel production purposes

$-??%. Triglycerides: gy main components from the lipid fraction Lipids are similar to those found in$

6 Population

7 International Airports México

8 Sun Irradiance - Mexico

9 Dry weigth (pg/cell) Nitrogen Limitation Peso celular (pg / célula) 0 [N] mm

10 Instituto de Biotecnología - UNAM 10

11 Instituto de Biotecnología - UNAM 11

12 Estándar interno: Vinil laureato Lipid Composition C16 C18

13 Lipids from micro-algae Algae + Sun + CO 2 + Minerals Cell Mass Nitrogen Limitation Lipid Acumulation 50% Lipids 0.5 g/l Lipids 35 mg LIPIDS /L/Day Instituto de Biotecnología - UNAM 13

14 Potential a l Lípidos Carbohydrates Proteins PRODUCTION Tons/ha/year 15 8 Nitrogen Limitation 32 Lipids Estimated biodiesel production 1 Ha Open ponds with 20 cm Height 80% TAGs 80% yield trans-sterification 90% production time during the year kg/l Biodiesel density 24,000 L BIODIESEL /Ha/year Instituto de Biotecnología - UNAM 14

Height 80% TAGs 80% yield trans-sterification 90% production time during the year 0.

15 Potential Lipids 15 Proteins 8 32 Carbohydrates PRODUCTION Tons/ha/year Phosphate Limitation Switch composition Instituto de Biotecnología - UNAM 15

16 PROPOSED PRODUCTION SYSTEM

17 Potential Biofuels from Microalge BIO-REFINARY a l Water + Nutrients Ligth CO 2 BIOMASS Glycerol Biodiesel Garibay-Hernández et al., 2009 Water + Nutrients CELL MASS ACUMULATION TRANSESTERIFICATION Alcohol CELL MASS CONCENTRATION Oils EXTRACCIÓN LIPÍDICA Turbosine And others Craking Microalgal Concentrate t CARBOHYDRATES, PROTEINS & OTHER MOLECULES Methane Electricity

, 2009 Water + Nutrients CELL MASS ACUMULATION TRANSESTERIFICATION Alcohol CELL MASS

18 Remarks a l Proper biofuels are needed for airplanes Mexico is in an appropriate sun irradiation area Microalge have a high photosynthetic efficiency and can be used to attain biofuels N. oleoabundas is an adequate Microalge for biofuel production purposes p Produces up to 55 ton/ha/year using simple conditions: bubbling only air; water plus salts; do not need extreme-high irradiance; some degree of contamination is not an issue Potential had been evaluated at laboratory scale Metabolism can be easily switched limiting the culture with different nutrients A biorefinery can be develop from this culture It is necessary to obtain the mass and energy balances to generate an economic study, and to develop certificated test at different scales: Laboratory Pilot (greenhouse) Demonstration Scale Instituto de Biotecnología - UNAM 18

some degree of contamination is not an issue Potential had been evaluated at laboratory scale Metabolism can be easily switched limiting the culture with different nutrients A biorefinery can be

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