MON Presentacion preparada por Tom Nickson 1

Transcripción

1 MON Presentacion preparada por Tom Nickson 1

2 >15% de media anual de perdida en climas tropicales y temperados El estrés por sequia es la mayor causa de perdida de rendimiento en maíz El mejoramiento usando técnicas de mejora convencionales puede ayudar Las técnicas de mejora brindan un aumento del rendimiento con el tiempo (~1% por año) Reduce la necesidad de laboreo y limita la evaporación en el suelo La biotecnología proporciona ayuda Mejora el rendimiento i moderadamente d bajo condiciones i de sequia No se esperan cambios en el área de producción 2

3 Conventional corn Water-limited during flowering Well-watered during flowering 3

4 nt Marker A B C FLAG-CSPB Co-IP Negative Control Co-IP 25nt Marker FLAG-CSPB Co-IP FLAG-CSPB Co-IP + RNase 25nt Marker CSPB binds RNA, but not dsdna, and unfolds RNA secondary structures consistent with bacterial CSPs and plant CSD-containing proteins FLAG-CSPB Co-IP FLAG-CSPB Co-IP + DNase Expression (pg CSPB/µg Total Protein) CSPB Basal Basal-Medial Medial-Distal Distal Accumulates in rapidly growing and developing regions of leaves and reproductive organs in MON On sub-cellular level CSPB localized to cytoplasm and nucleus Accumulation and localization pattern is similar to what is known for other CSD-containing proteins found in plants PS2 Quantum Efficiency (ΦII; Fm' Fs/Fm') Control MON PM AM PM PM PM PM 5/16/2008 5/19/2008 5/20/2008 5/21/2008 5/22/2008 T0 T1 T2 T3 T4 Under water-limited conditions plants expressing CSPB exhibit improved vegetative productivity :Improvements in: CO 2 assimilation Stomatal conductance Trends toward improved: Leaf extension rate Overall growth rate Minimal effect on final plant height Physiological functions are less impacted during water limitation Reduced yield loss under water-limited conditions Improved physiological capacity during drought allows greater yields General RNA Chaperone Activity of CSPB proteins in MON

5 Plan de análisis Caracterización del producto Caracterización molecular y análisis de expresión Caracterización de la proteína introducida Ausencia de toxicidad y alergenicidad Análisis composicional Caracterización de la planta bajo condiciones de estrés por falta de agua y condiciones sin estrés en múltiples localidades durante dos anos. Estudios en el campo para estudiar la supervivencia y competitividad en ambientes fuera de ;a producción agrícola Estudios confirmatorios con animales y microorganismos confirmando la ausencia de toxicidad 5

6 5 end P-ract Ract1 intron csp Tr7 lox P-35S nptii NOS lox 3 end Transformado con Agrobacterium Un único sitio de inserción de T-DNA Una sola copia de los casets cspb y nptii No otros elementos o secuencias bacterianas nptii caset rodeado por lox-p sites Estabilidad generacional nptii se considera seguro para la salud humana, animal y medio ambiental Segregación esperada Expresión estudiada en varios tejidos y 3 estados de crecimiento 6

7 Mean Reference Range 1 Phenotypic characteristic Units MON Control Min Max Seedling vigor 0-9 scale Early stand count #/plot Days to 50% pollen shed Days Days to 50% silking Days Stay green 0-9 scale Ear height in Plant height in Dropped ears 4 #/plot Stalk lodged plants 4 #/plot Root lodged plants 4 #/plot Final stand count #/plot Grain moisture % Test weight lbs/bushel Yield bu/a Note: No statistically significant differences were detected (p<0.05) between the test and control substance. 1 Reference range was calculated from the 3 sites exhibiting a water treatment effect among the 12 reference corn hybrids. 2 Seedling vigor rating scale: 0 = dead and 9 = above average vigor. 3 Stay green rating scale: 0 = entire plant is dried and 9 = entire plant is green. 4 No statistical comparisons were made due to lack of variability in the data. The test was considered effectively not different from the control because the test and control mean values were identical. 7

8 Mejora bajo condiciones de sequia Mean Reference Range 1 Phenotypic characteristic Units MON Control Min Max Seedling vigor 0-9 scale Early stand count #/plot Days to 50% pollen shed Days Days to 50% silking Days Stay green 0-9 scale Ear height in Plant height in Dropped ears 4 #/plot Stalk lodged plants 4 #/plot Root lodged plants 4 #/plot Final stand count #/plot Grain moisture % Test weight lbs/bushel Yield bu/a 114.5* * Indicates statistical difference between the test and the control (p 0.05). 1 Reference range was calculated from the 3 sites exhibiting a water treatment effect among the 12 reference corn hybrids. 2 Seedling vigor rating scale: 0 = dead and 9 = above average vigor. 3 Stay green rating scale: 0 = entire plant is dried and 9 = entire plant is green. 4 No statistical comparisons were made due to lack of variability in the data. The test was considered effectively not different from the control because the test and control mean values were identical. 8

9 Dormancia y Germinacion Mean % Reference Range Temperature Characteristic MON Control Min Max 5 C Dead Germinated Viable Firm Swollen Viable Hard Dead Germinated C Viable Firm Swollen 20 C 30 C N = 400 seed (100/rep) Viable Hard Dead Germinated Viable Firm Swollen Viable Hard Dead Germinated Viable Firm Swollen Viable Hard

10 Potencial de voluntarios Semillas plantadas en el otoño del 2006 se estudiaron en el otoño y la primavera del 2007 en tres localidades Resultados No se observaron voluntarios 10

11 MON no es persistente El grano de MON no sobrevive el invierno y no hay voluntarios MON no exhibe dormancia diferente en comparación a maíz convencional MON no tiene una susceptibilidad d diferente a insectos o patógenos MON no altera la abundancia de plagas o artrópodos beneficiales i La viabilidad del polen de MON no es diferente a la del polen de maíz convencional 11