FURTHER STUDIES ABOUT THE PATHOGENESIS OF THE PISCIRICKETTSIOSIS (SALMONID RICKETTSIAL SEPTICAEMIA) IN

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1 CONICYT: Repositorio Institucional: Ficha de Iniciativa de CIT (Ciencia, Tecnología e Innovación) 1 FONDECYT-REGULAR FURTHER STUDIES ABOUT THE PATHOGENESIS OF THE PISCIRICKETTSIOSIS (SALMONID RICKETTSIAL SEPTICAEMIA) IN SALMONID FISH CAUSED BY PISCIRICKETTSIA SALMONIS Programa: Fondecyt Regular The first worldwide report of "Salmonid Rickettsial Septicaemia" or "Piscirickettsiosis" was made in Chile in This disease caused by Piscirickettsia salmonis, an obligate intracellular bacterium, which since its detection to the present has resulted in extensive losses to the national aquaculture. The pathogenic mechanisms, currently very little known, may constitute a critical information to sustain immunoprophylactic and therapeutic methods effective for the disease control. The purpose of this project is to carry out research work to contribute to the understanding of the initial events of the pathogeny of this disease. The general objective is to analyze the initial processes of the pathogeny, particularly by studying the phenomena of penetration through the skin, gills and intestines and its subsequent distribution in the different organs of salmonid fish. The techniques of confocal scanning and immunoultrastructural analysis by electronic transmission microscopy will be adapted to determine, specifically and with great resolution, the presence of P. salmonis in cells and tissues. Likewise, the method of polymerase chain reaction will be implemented, which will allow this study to be carried out with this high-sensitivity and specificity technique.different systems of experimental infection will be applied, including intraperitoneal injections, intestinal intubation of fish with suspensions of the rickettsial agent as well as skin and gill infections with pathogen-imbibed paper patches. In a first stage the mechanisms of bacterial entry will be observed by means of confocal and electronic microscopy and then the sequential access and distribution in time in the different internal organs will be determined by means of PCR. Both the bacterial entry and early detection of access to different white organs will also be analyzed by histopathology, at the optical microscopy level, to observe the associated lesions. Antecedentes # Nombre : FURTHER STUDIES ABOUT THE PATHOGENESIS OF THE PISCIRICKETTSIOSIS (SALMONID RICKETTSIAL SEPTICAEMIA) IN SALMONID FISH CAUSED BY PISCIRICKETTSIA SALMONIS # Número : # Año Concurso : 2001 # Concurso : FONDECYT-REGULAR

2 CONICYT: Repositorio Institucional: Ficha de Iniciativa de CIT (Ciencia, Tecnología e Innovación) 2 # Consejo : TECNOLOGIA # Duración : 3 años # Estado : APROBADO # Sector de Aplicación : The first worldwide report of "Salmonid Rickettsial Septicaemia" or "Piscirickettsiosis" was made in Chile in This disease caused by Piscirickettsia salmonis, an obligate intracellular bacterium, which since its detection to the present has resulted in extensive losses to the national aquaculture. The pathogenic mechanisms, currently very little known, may constitute a critical information to sustain immunoprophylactic and therapeutic methods effective for the disease control. The purpose of this project is to carry out research work to contribute to the understanding of the initial events of the pathogeny of this disease. The general objective is to analyze the initial processes of the pathogeny, particularly by studying the phenomena of penetration through the skin, gills and intestines and its subsequent distribution in the different organs of salmonid fish. The techniques of confocal scanning and immunoultrastructural analysis by electronic transmission microscopy will be adapted to determine, specifically and with great resolution, the presence of P. salmonis in cells and tissues. Likewise, the method of polymerase chain reaction will be implemented, which will allow this study to be carried out with this high-sensitivity and specificity technique. Different systems of experimental infection will be applied, including intraperitoneal injections, intestinal intubation of fish with suspensions of the rickettsial agent as well as skin and gill infections with pathogen-imbibed paper patches. In a first stage the mechanisms of bacterial entry will be observed by means of confocal and electronic microscopy and then the sequential access and distribution in time in the different internal organs will be determined by means of PCR. Both the bacterial entry and early detection of access to different white organs will also be analyzed by histopathology, at the optical microscopy level, to observe the associated lesions. Resultados EVENTOS # CINETICA DE LA INFECCION DE PISCIRICKETTIS SALMONIS IN VITRO MEDIANTE MICROSCOPIA ELECTRONICA DE TRANSMISION, IMMUNOGOLD Y MICROSCOPIA LASER CONFOCAL, En: 7º ENCONTRO BRASILEIRO DE PATOLOGISTAS DE ORGANISMOS ACUATICOS. BRASIL

3 CONICYT: Repositorio Institucional: Ficha de Iniciativa de CIT (Ciencia, Tecnología e Innovación) 3 # REVECO, FELIPE # VENEGAS, CLAUDIA. # EARLY EVENTS OF THE INFECTION OF PISCIRICKETTSIA SALMONIS OF CHSE-214 CELLS BY CONFOCAL MICROSCOPY, En: 4 INTERNATIONAL SYMPOSIUM ON AQUATIC ANIMAL HEALTH. NEW ORLEANS, ESTADOS UNIDOS DE AMERICA # VENEGAS, CLAUDIA # EVENTOS INICIALES DE LA PATOGENIA DE PISCIRICKETTSIA SALMONIS, AGENTE CAUSAL DE LA PIS IRICKETTSIOSIS EN SALMON COHO (ONCORHYNCUS MYKISS), En: 7º ENCONTRO BRASILEIRO DE PATOLOGISTAS DE ORGANISMOS ACUATICOS. BRASIL # ESTAY, DANIEL # ROJAS, MARIA E. # EXPERIMENTAL INFECTION OF COHO SALMON (ONCORHYNCHUS KISUTCH) EXPOSING THE SURFACE OF SKIN, GILLS AND INSTESTINE WITH PISCIRICKETTSIA SALMONIS, En: 4 INTERNATIONAL SYMPOSIUM

4 CONICYT: Repositorio Institucional: Ficha de Iniciativa de CIT (Ciencia, Tecnología e Innovación) 4 ON AQUATIC ANIMAL HEALTH. NEW ORLEANS, ESTADOS UNIDOS DE AMERICA # ESTAY, DANIEL # ROJAS, MARIA E # EXPERIMENTAL REPRODUCTION OF PISCIRICKETTSIOSIS BY CHALLENGING RAINBOW TROUT (ONCORHYNCHUS MYKISS)BY AN IMMERSION BATH WITH PISCIRICKETTSIA SALMONIS, En: 11 INTERNATIONAL CONFERENCE OF THE EUROPEAN ASSOCIATION OF FISH PATHOLOGIST. MALTA # OJEDA, PABLO # PIZARRO, PATRICIA. # INFECTIVITY STUDY OF PISCIRICKETTSIA SALMONIS IN CHSE-214 CELLS BY IMMUNOGOLD AND STANDARD TRANSMISSION ELECTRON MICROSCOPY, En: 4 INTERNATIONAL SYMPOSIUM ON AQUATIC ANIMAL HEALTH. NEW ORLEANS, ESTADOS UNIDOS DE AMERICA

5 CONICYT: Repositorio Institucional: Ficha de Iniciativa de CIT (Ciencia, Tecnología e Innovación) 5 # REVECO, FELIPE # PENETRACION DE PISCIRICKETTSIA SALMONIS A OVAS DE TRUCHA ARCO IRIS (ONCORHYNCHUS MYKISS) ESTUDIADO MEDIANTE MICROSCOPIA DE BARRIDO, En: 7º ENCONTRO BRASILEIRO DE PATOLOGISTAS DE ORGANISMOS ACUATICOS. BRASIL # FERNANDEZ, SOLEDAD # LEDEZMA, HUMBERTO. # PRELIMINARY RESULTS OF AN INFECTIVITY STUDY OF PISCIRICKETTSIA SALMONIS BY CONFOCAL SCANNING MICROSCOPY IN CHSE-214 CELLS, En: 10 INTERNATIONAL CONFERENCE OF THE EUROPEAN ASSOCIATION OF FISH PATHOLOGIST. DUBLIN, IRLANDA # GUAJARDO, A # VENEGAS, C. # VERTICAL TRANSMISSION OF PISCIRICKETTSIA SALMONIS AND A STUDY OF THE MODE OF ENTRANCE INTO THE OVUM, En: 4 INTERNATIONAL SYMPOSIUM ON AQUATIC ANIMAL HEALTH. NEW ORLEANS, ESTADOS UNIDOS DE AMERICA

6 CONICYT: Repositorio Institucional: Ficha de Iniciativa de CIT (Ciencia, Tecnología e Innovación) 6 # FERNANDEZ, SOLEDAD # LEDEZMA, HUMBERTO # NIEVES, SANDOVAL # TRONCOSO, OSCAR INFORME FINAL # FURTHER STUDIES ABOUT THE PATHOGENESIS OF THE PISCIRICKETTSIOSIS (SALMONID RICKETTSIAL SEPTICAEMIA) IN SALMONID FISH CAUSED BY PISCIRICKETTSIA SALMONIS, p. MANUSCRITOS # A DNA-DNA HYBRIDIZATION ASSAY FOR THE DETECTION ON PISCIRICKETTSIA SALMONIS FROM SALMONIDS, # VENEGAS, C.

7 CONICYT: Repositorio Institucional: Ficha de Iniciativa de CIT (Ciencia, Tecnología e Innovación) 7 # EXPERIMENTAL INFECTION OF COHO SALMON (ONCORHYNCHUS KISUTCH) BY EXPOSUR OF SKIN, GILLS AND INTESTINE WITH PISCIRICKETTSIA SALMONIS, p. # ESTAY, D # MORALES, M A # ROJAS, MARIA E. # INFECTIVITY STUDY OF PISCIRICKETTSIA SALMONIS IN CHSE-214 CELLS BY CONFOCAL AND TRANSMISSION ELECTRON MICROSCOPY, 17 p.. # REVECO, FELIPE # VENEGAS, CLAUDIA. TESIS