Supplementary Table 1: List of the 316 genes regulated during hyperglycemic euinsulinemic clamp in skeletal muscle.

Transcripción

1 Supplementary Table 1: List of the 316 genes regulated during hyperglycemic euinsulinemic clamp in skeletal muscle. UGCluster Name Symbol Fold Change Cytoband Response to stress Hs Heme oxygenase (decycling) 1 HMOX q12 Hs Metallothionein 1X MT1X q13 Hs Metallothionein 1B (functional) MT1B q13 Hs Oxidation resistance 1 OXR q23 Hs Metallothionein 1F (functional) MT1F q13 Hs Metallothionein 2A MT2A q13 Hs Metallothionein 1H MT1H q13 Hs Glutathione S-transferase pi GSTP q13 Hs Stannin SNN p13 Immune response, cytokines & related Hs TNF (ligand) superfamily, member 10 (TRAIL) TNFSF q26 Hs CD59 antigen p18-20 (protectin) CD p13 Hs Complement component 4B, telomeric C4A p21.3 Hs Immunoglobulin lambda variable 6-57 IGLV q11.2 Hs Calcium modulating ligand CAMLG q23 Hs B-cell CLL/lymphoma 10 BCL p22 Hs.840 Indoleamine-pyrrole 2,3 dioxygenase INDO p12-p11 Hs Mal, T-cell differentiation protein 2 MAL Hs CD99 antigen-like 2 CD99L Xq28 Hs Chemokine (C-C motif) ligand 19 CCL p13 Hs Interferon regulatory factor 2 binding protein 2 IRF2BP q42.3 Hs Contactin 1 CNTN q11-q12 Hs MHC class I mrna fragment p21.3 Hs Carcinoembryonic antigen-related cell adhesion molecule 19 CEACAM q13.31 Hs Selectin E (endothelial adhesion molecule 1) SELE q22-q25 Hs Family with sequence similarity 19 (chemokine (C-C motif)-like), member A5 FAM19A q13.32 Hs Fc fragment of IgG, low affinity IIIb, receptor (CD16b) FCGR3B q23 Hs Junctional adhesion molecule 2 JAM q21.2 Hs S100 calcium binding protein A12 (calgranulin C) S100A q21 Hs Nuclear factor of activated T-cells NFATC q23 Hs Deleted in colorectal carcinoma DCC q21.3 Hs Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D SEMA4D q22-q31 Circulating active peptides Hs.2561 Nerve growth factor, beta polypeptide NGFB p13.1 Hs Transforming growth factor, beta 2 TGFB q41 Hs Stanniocalcin 1 STC p21-p11.2 Hs Vascular endothelial growth factor C VEGFC q34.1-q34.3 Hs Coagulation factor II (thrombin) F p11-q12 Receptors Hs Patched homolog (Drosophila) PTCH q22.3 Hs Tumor-associated calcium signal transducer 2 TACSTD p32-p31 Hs Smoothened homolog (Drosophila) SMO q32.3 Hs Low density lipoprotein receptor (familial hypercholesterolemia) LDLR p13.3 Hs Integrin, beta 7 ITGB q13.13 Hs Adrenergic, alpha-1a-, receptor ADRA1A p21-p11.2 Hs Taste receptor, type 2, member 49 TAS2R p13.2 Hs Endothelial differentiation, lysophosphatidic acid G-proteincoupled receptor, 2 EDG q31.3 Hs.1041 Proto-oncogene tyrosine-protein kinase ROS precursor ROS q22 Hs Transmembrane protein 71 TMEM q24.22 Hs Poliovirus receptor-related 3 PVRL q13 Hs Prostaglandin E receptor 3 (subtype EP3) PTGER p31.2 Hs Receptor tyrosine kinase-like orphan receptor 2 ROR q22 Signaling pathways Protein phosphatases Hs Protein phosphatase 1, catalytic subunit, gamma isoform PPP1CC q24.1-q24.2 Hs Protein tyrosine phosphatase-like A domain containing 1 PTPLAD q22.2 Hs Protein tyrosine phosphatase, receptor type, O PTPRO p13.3-p13.2 Hs Protein tyrosine phosphatase, non-receptor type 14 PTPN q32.2 Hs Protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), alpha isoform PPP2R2A p21.2 Hs Protein phosphatase 1, regulatory subunit 10 PPP1R p21.3 Protein kinases Hs Mitogen-activated protein kinase 1 MAPK q11.2 Hs Protein kinase D2 PRKD q13.3 Hs Cyclin-dependent kinase-like 3 CDKL q31 Hs TAO kinase 2 TAOK p11.2 Hs Tyrosine-protein kinase HCK DDEF q24.1-q24.2 Hs Cyclin-dependent kinase 6 CDK q21-q22

2 Hs LIM domain kinase 1 LIMK q11.23 Hs AP2 associated kinase 1 AAK p24.3-p14 Others signaling pathways Hs Homer homolog 1 (Drosophila) HOMER q14.2 Hs Calcyphosine CAPS p13.3 Hs FYVE, RhoGEF and PH domain containing 1 (faciogenital dysplasia) FGD Xp11.21 Hs Calneuron 1 CALN q11 Hs GRB2-associated binding protein 2 GAB q14.1 Hs Secreted frizzled-related protein 1 SFRP p12-p11.1 Hs Pleckstrin homology domain containing, family A member 5 PLEKHA p12 Hs SH3 domain and tetratricopeptide repeats 2 SH3TC q32 Hs Ras-like without CAAX 2 RIT q12.3 Hs Centaurin, alpha 1 CENTA p22.3 Hs Arrestin, beta 2 ARRB p13 Hs Insulin receptor substrate 1 IRS q36 Hs Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha NFKBIA q13 Hs Ectonucleotide pyrophosphatase/phosphodiesterase 2 (autotaxin) ENPP q24.1 Hs SH3-domain GRB2-like (endophilin) interacting protein 1 SGIP p31.3 Hs.2722 Inositol 1,4,5-trisphosphate 3-kinase A ITPKA q14-q21 Hs Rap guanine nucleotide exchange factor (GEF) 2 RAPGEF q32.1 Hs GTP-binding protein PTD004 PTD q31.1 Hs RalA binding protein 1 RALBP p11.3 Hs RAB27A, member RAS oncogene family RAB27A q15-q21.1 Hs Regulator of G-protein signalling 4 RGS q23.3 Hs Phosphodiesterase 6A, cgmp-specific, rod, alpha PDE6A q31.2-q34 Hs Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma IKBKG Xq28 Hs Phosphoprotein enriched in astrocytes 15 PEA q21.1 Hs Rho GTPase activating protein 29 ARHGAP p22.1 Enzymes Carbohydrate metabolism Hs Aldolase A, fructose-bisphosphate ALDOA q22-q24 Hs Phosphorylase, glycogen; muscle (McArdle syndrome, glycogen storage disease type V) PYGM q12-q13.2 Hs Phosphoglucomutase 3 PGM q14.1-q15 Hs Phosphorylase kinase, alpha 2 (liver) PHKA Xp22.2-p22.1 Hs Fructosamine-3-kinase-related protein FN3KRP q25.3 Hs.8364 Pyruvate dehydrogenase kinase, isoenzyme 4 PDK q21.3-q22.1 Lipid metabolism Hs Acetyl-Coenzyme A carboxylase beta ACACB q24.1 Hs Monoacylglycerol O-acyltransferase 3 MOGAT q22.1 Hs Sterol-C4-methyl oxidase-like SC4MOL q32-q34 Hs Arachidonate 15-lipoxygenase, second type ALOX15B p13.1 Hs Lipase, member H LIPH q27 ATPase & related Hs ATPase, Na+/K+ transporting, beta 1 polypeptide ATP1B q24 Hs ATPase, Class I, type 8B, member 1 ATP8B q21-q22 Others enzymes Hs Creatine kinase, muscle CKM q13.2-q13.3 Hs Alanine-glyoxylate aminotransferase (oxalosis I; hyperoxaluria I; glycolicaciduria; serine-pyruvate aminotransferase) AGXT q36-q37 Hs Guanosine monophosphate reductase GMPR p23 Hs N-acetylgalactosaminidase, alpha- NAGA q13-qter Hs ,10-methylenetetrahydrofolate reductase (NADPH) MTHFR p36.3 Hs Collapsin response mediator protein 1 CRMP p16.1-p15 Hs Dehydrogenase/reductase (SDR family) member 2 DHRS q11.2 Structural proteins & cytoskeleton Hs Actin, gamma 2, smooth muscle, enteric ACTG p13.1 Hs Kinesin 2 KNS q32.3 Hs Nexilin (F actin binding protein) NEXN p31.1 Hs Myosin binding protein C, slow type MYBPC q23.3 Hs Titin TTN q31 Hs EF hand domain family, member D1 EFHD q37.1 Hs Collagen, type IX, alpha 1 COL9A q12-q14 Hs Collagen, type IV, alpha 5 (Alport syndrome) COL4A Xq22 Hs Plakophilin 4 PKP q23-q31 Hs Keratin 5b K5B q13.13 Hs Leucine-rich repeats and calponin homology (CH) domain containing 3 LRCH q29 Hs Microtubule-associated protein, RP/EB family, member 2 MAPRE q12.1 Hs Nidogen 2 (osteonidogen) NID q21-q22 Hs Myosin VA (heavy polypeptide 12, myoxin) MYO5A q21

3 Hs Tubulin tyrosine ligase-like family, member 2 TTLL q27 Hs Spectrin, beta, non-erythrocytic 1 SPTBN p21 Hs Tubulin, alpha 6 TUBA q12-q14 Hs Growth arrest-specific 8 GAS q24.3 Hs Doublecortex; lissencephaly, X-linked (doublecortin) DCX Xq22.3-q23 Hs Claudin 3 CLDN q11.23 Hs Sarcoglycan, delta (35kDa dystrophin-associated glycoprotein) SGCD q33-q34 Hs Neurofibromin 2 (bilateral acoustic neuroma) NF q12.2 Hs Erythrocyte membrane protein band 4.1 EPB p33-p32 Hs Ectodermal-neural cortex (with BTB-like domain) ENC q12-q13.3 Hs Myosin VB MYO5B q21 Hs Laminin, gamma 1 (formerly LAMB2) LAMC q31 Hs Collagen, type VI, alpha 2 COL6A q22.3 Transporters & carriers Hs Fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor) FABP p33-p32 Hs Solute carrier family 43, member 2 SLC43A p13.3 Hs Neuroglobin NGB q24 Hs Solute carrier family 2 (facilitated glucose transporter), member 12 SLC2A q23.2 Hs Synaptosomal-associated protein, 25kDa SNAP p12-p11.2 Hs Trans-golgi network protein 2 TGOLN p11.2 Hs Sodium channel, nonvoltage-gated 1, delta SCNN1D p36.3-p36.2 Hs Solute carrier family 2 (facilitated glucose transporter), member 2 SLC2A q26.1-q26.2 Hs Stomatin (EPB72)-like 1 STOML q24-q25 Hs Solute carrier family 19 (thiamine transporter), member 2 SLC19A q23.3 Hs Solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 SLC1A p24 Hs Syntaxin binding protein 6 (amisyn) STXBP q12 Hs Potassium large conductance calcium-activated channel, subfamily M, beta member 1 KCNMB q34 Hs Hyperpolarization activated cyclic nucleotide-gated potassium channel 4 HCN q24-q25 Hs Lipocalin 2 (oncogene 24p3) LCN q34 Transcription and DNA related proteins Transcription factors and transcription regulation Hs TSC22 domain family, member 1 TSC22D q14 Hs SET and MYND domain containing 1 SMYD p11.2 Hs Forkhead box O1A (rhabdomyosarcoma) FOXO1A q14.1 Hs Zinc finger protein 281 ZNF q32.1 Hs Retinoid X receptor, alpha RXRA q34.3 Hs Four and a half LIM domains 3 FHL p34 Hs Four and a half LIM domains 1 FHL Xq26 Hs SMAD, mothers against DPP homolog 5 SMAD q31 Hs General transcription factor IIB GTF2B p22-p21 Hs Yip1 interacting factor homolog B YIF1B q13.2 Hs Myeloid/lymphoid or mixed-lineage leukemia MLL q23 Hs Zinc finger protein 187 ZNF p21.31 Hs Orthopedia homolog (Drosophila) OTP q13.3 Hs Basic leucine zipper transcription factor, ATF-like BATF q24.3 Hs NK2 transcription factor related, locus 2 (Drosophila) NKX pter-q11.23 Hs AF4/FMR2 family, member 3 AFF q11.2-q12 Hs Zinc finger protein 81 (HFZ20) ZNF Xp11.23 Hs Nuclear factor of activated T-cells, cytoplasmic, calcineurindependent 3 NFATC q22.2 Hs Iroquois homeobox protein 5 IRX q11.2-q13 Hs Retinoblastoma binding protein 8 RBBP q11.2 Hs Nuclear factor I/X (CCAAT-binding transcription factor) NFIX p13.3 Hs.1545 Caudal type homeo box transcription factor 1 CDX q31-q33 Hs Period homolog 1 (Drosophila) PER p p12 Hs T-cell leukemia, homeobox 2 TLX p13.1-p12 Hs Transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A) TCEB p36.1 Hs Zinc finger protein 138 (clone phz-32) ZNF q11.21-q11.23 Hs AT rich interactive domain 5B (MRF1-like) ARID5B q21.2 Hs Immediate early response 5 IER q25.3 Hs Zinc finger protein 549 ZNF q13.43 Hs V-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) MYCN p24.1 Hs Paired box gene 5 (B-cell lineage specific activator) PAX p13 Hs V-myb myeloblastosis viral oncogene homolog (avian) MYB q22-q23 DNA associated proteins Hs Topoisomerase (DNA) II beta 180kDa TOP2B p24 Hs Proliferation-inducing protein 38 RP11-301I q14.11 Hs MRE11 meiotic recombination 11 homolog A (S. cerevisiae) MRE11A q21 Hs Additional sex combs like 1 (Drosophila) ASXL q11.1 Hs Postmeiotic segregation increased 2-like 4 PMS2L q11-q22

4 Hs ZXD family zinc finger C ZXDC q21.2 Hs Topoisomerase (DNA) III beta TOP3B q11.22 Hs Histone 1, H2al HIST1H2AL p22-p21.3 Translation RNA transport and processing Hs Muscleblind-like (Drosophila) MBNL q25 Hs Heterogeneous nuclear ribonucleoprotein A0 HNRPA q31 Hs Poly(A) polymerase gamma PAPOLG p16.1 Hs Nuclear RNA export factor 1 NXF q12-q13 Hs TIA1 cytotoxic granule-associated RNA binding protein TIA p13 Hs Nucleoporin-like protein RIP HRB q36.3 Hs Apobec-1 complementation factor ACF q11.23 Hs LSM6 homolog, U6 small nuclear RNA associated (S. cerevisiae) LSM q31.22 Translation and post-translational modifications Hs Transmembrane and tetratricopeptide repeat containing 1 TMTC p11.22 Hs Glycosyltransferase 8 domain containing 1 GLT8D p21.1 Hs Ribosomal protein L30 RPL q22 Hs Ribosomal protein S15a RPS15A p Hs Arginyltransferase 1 ATE q26.13 Hs Lysyl oxidase-like 2 LOXL p21.3-p21.2 Hs UDP-glucose ceramide glucosyltransferase-like 1 UGCGL q14.3 Hs Dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit DPM q13.13 Hs Threonyl-tRNA synthetase TARS p13.2 Hs Eukaryotic elongation factor, selenocysteine-trna-specific EEFSEC q21.3 Hs Mannose-P-dolichol utilization defect 1 MPDU p13.1-p12 Hs UDP-glucose ceramide glucosyltransferase-like 2 UGCGL q32.1 Hs Mannosyl (beta-1,4-)-glycoprotein beta-1,4-nacetylglucosaminyltransferase MGAT q13.1 Hs Phosphatidylinositol N-acetylglucosaminyltransferase GPI1 subunit PIGQ p13.3 Hs Eukaryotic translation initiation factor 2-alpha kinase 2 EIF2AK p22-p21 Protein catabolism Ubiquitin-proteasome pathway Hs Ubiquitin specific protease 13 (isopeptidase T-3) USP q26.2-q26.3 Hs Ring finger protein 43 RNF q22 Hs Cullin-associated and neddylation-dissociated 1 CAND q14 Proteases and protease inhibitors Hs Proprotein convertase subtilisin/kexin type 1 PCSK q15-q21 Hs Matrix metalloproteinase 19 MMP q14 Hs Prolyl endopeptidase PREP q22 Hs Inter-alpha (globulin) inhibitor H2 ITIH p15 Hs Reelin RELN q22 Hs Crm, cramped-like (Drosophila) CRAMP1L p13.3 Hs Cathepsin O CTSO q31-q32 Hs Protease, serine, 12 (neurotrypsin, motopsin) PRSS q28.1 Others and proteins with unknown function Hs Putative lymphocyte G0/G1 switch gene G0S q32.2-q41 Hs LIM and cysteine-rich domains 1 LMCD p26-p24 Hs Arsenate resistance protein ARS2 ARS q21 Hs Transmembrane protein 125 TMEM p34.2 Hs Downregulated in ovarian cancer 1 DOC q12.1 Hs Ankyrin repeat domain 36 ANKRD q11.2 Hs Vitamin K epoxide reductase complex, subunit 1-like 1 VKORC1L q11.21 Hs Transmembrane protein 80 TMEM p15.5 Hs Mast cell-expressed membrane protein 1 MCEMP p13.2 Hs Endomucin EMCN q24 Hs Kin of IRRE like (Drosophila) KIRREL q21-q25 Hs Butyrophilin, subfamily 3, member A3 BTN3A p21.3 Hs Male sterility domain containing 1 MLSTD p11.22 Hs Fasciculation and elongation protein zeta 1 (zygin I) FEZ q24.2 Hs Family with sequence similarity 38, member B FAM38B p11.22 Hs WD repeat domain 73 WDR q25.2 Hs Myelin oligodendrocyte glycoprotein MOG p22.1 Hs Family with sequence similarity 32, member A FAM32A pter-p13.3 Hs Growth hormone regulated TBC protein 1 GRTP q34 Hs Neuron navigator 1 NAV Hs Transmembrane protein 115 TMEM p21.3 Hs Torsin A interacting protein 1 TOR1AIP q24.2 Hs Acrosomal vesicle protein 1 ACRV p12-q13 Hs WD repeat domain 22 WDR q23-q24.1 Hs Translocation associated membrane protein 1-like 1 TRAM1L q26 Hs Zinc finger, DHHC domain containing 14 ZDHHC q25.3 Hs Major facilitator superfamily domain containing 7 MFSD p16.3 Hs Huntingtin interacting protein E HYPE q24.1

5 Hypothetical proteins and EST Hs Hypothetical protein LOC LOC q13.1 Hs Transcribed locus 1.93 Hs Transcribed locus q35.3 Hs Hypothetical protein LOC LOC q24.22 Hs Transcribed locus 1.40 Hs Chromosome 20 open reading frame 117 C20orf q11.23 Hs Clone mrna sequence Hs Transcribed locus Hs hypothetical protein LOC q13.11 Hs FLJ16124 protein FLJ p14 Hs Hypothetical protein MGC26694 MGC p13.11 Hs Chromosome 5 open reading frame 3 C5orf q31-q33 Hs Chromosome 6 open reading frame 165 C6orf q15 Hs.4988 Hypothetical protein LOC LOC q21.3 Hs Chromosome 15 open reading frame 40 C15orf q25.2 Hs Transcribed locus Hs Transcribed locus Hs Hypothetical protein MGC10646 MGC q21.21 Hs hypothetical protein H q24.2 Hs Transcribed locus Hs Transcribed locus Hs Transcribed locus Hs Transcribed locus Hs Transcribed locus Hs.8429 Transcribed locus Hs Transcribed locus Hs Transcribed locus Hs Hypothetical gene supported by AK LOC q27 Hs chromosome 17 open reading frame p11.2 Hs Transcribed locus Hs Transcribed locus Hs Hypothetical BC331191_1 LOC q13.2 Hs Transcribed locus] Hs.6224 KIAA0895 protein KIAA p14.2 Hs Hypothetical protein FLJ12700 FLJ q36.1 Hs.9003 Chromosome 16 open reading frame 58 C16orf p11.2 Hs Full-length cdna clone CS0DC009YH Hs Transcribed locus Hs Transcribed locus Hs KIAA0922 protein KIAA q31.3 Hs Transcribed locus Hs Transcribed locus Hs Hypothetical protein FLJ40243 FLJ p13.1 Hs Hypothetical gene supported by AK q34.11 Hs Transcribed locus Hs Hypothetical protein LOC LOC p23.3 Hs Hypothetical protein FLJ13231 FLJ p13.2 Hs Transcribed locus Hs Transcribed locus Hs Hypothetical protein LOC LOC q36.1 Hs Full-length cdna clone CS0DK005YO Hs Transcribed locus Hs Hypothetical protein MGC35048 MGC p12.3 Hs Transcribed locus Hs Transcribed locus Hs KIAA1217 KIAA p12.1 Hs Transcribed locus Hs Transcribed locus Hs Hypothetical protein MGC24381 MGC p13.3 Hs hypothetical protein FLJ32949 FLJ q14.2 Hs Chromosome Y open reading frame 15B CYorf15B Yq Hs Transcribed locus Hs Hypothetical protein FLJ20366 FLJ q23.2 Hs Transcribed locus, -2.34