La ciencia nunca frena, nosotros tampoco.

Transcripción

1

2 La ciencia nunca frena, nosotros tampoco. Nuestros productos y servicios de biotecnología son creados y avalados por científicos. Profesionales en más de 160 países utilizan nuestras innovadoras soluciones en su afán por resolver algunos de los desafíos científicos más difíciles del siglo XXI. En Life Technologies estamos orgullosos de unirnos a ellos en su búsqueda por acelerar los descubrimientos científicos que mejoran la vida. Encontrará en nuestra compañía un portfolio completo de productos para ADN, ARN, Proteínas, Cultivo Celular e instrumentos. Con más de 230 productos en stock permanente en nuestros depósitos, el presente catálogo pretende atender con mayor agilidad las necesidades de los investigadores. Información para ordenar Solicite un presupuesto vía a arorders@lifetech.com o a través de nuestra página web en invitrogen.com.ar/public_html/invitrogen - Consulta de Precios.html

3 Índice de contenido Cultivo Celular PCR, RT-PCR y qpcr Reactivos y consumibles para secuenciadores automáticos Placas, Tubos, Strip de tubos, Tapas y Film Purificación, Cuantificación y Tinción de Ácidos Nucleicos Ultrapuros Marcadores Clonado Enzimas de Restricción y Modificadoras Reactivos para experimentos inmunológicos Instrumentos

4 Biostock CULTIVO CELULAR Advanced DMEM Invitrogen Ampicillin, Sodium Salt 200 mg Antibiotic-Antimycotic, 100X Antibiotic-Antimycotic, 100X 20 ml DMEM, High Glucose 10x DMEM, High Glucose DMEM, High Glucose, GlutaMAX DMEM, High Glucose, Pyruvate DMEM, Low Glucose, Pyruvate DMEM, Powder, High Glucose DMEM, Powder, High Glucose, Pyruvate DMEM, Powder, High Glucose, Pyruvate DMEM, Powder, Low Glucose, Pyruvate DMEM/F-12, HEPES 10x DMEM/F-12, Powder, HEPES DPBS, Powder, no Calcium, no Magnesium Fungizone Antimycotic, Liquid 20 ml Geneticin Selective Antibiotic, Powder 1 gr Gentamicin Reagent Solution (10 mg/ml), Liquid 10 ml Gentamicin Reagent Solution (50 mg/ml), Liquid 10 ml A GlutaMAX -I CTS Ham's F-10 Nutrient Mix Ham's F-10 Nutrient Mix, HEPES Ham's F-12 Nutrient Mix Ham's F-12 Nutrient Mix, powder HBSS, 10X, no Calcium, no Magnesium, no Phenol Red HEPES Buffer Solution (1 M) Invitrogen Hygromycin B, 50 mg/ml Solution 20 ml Invitrogen Kanamycin Sulfate 5 gr KaryoMAX Colcemid Solution, Liquid (10 µg/ml), in PBS 10 ml L-Glutamine-200 mm (100X), Liquid 3

5 PAG. 11 PAG. 4 Biostock 2013 CULTIVO CELULAR ULTRAPUROS 11 Código Marca Gibco Detalle Presentación Código Marca Detalle Presentación UltraPure Acrylamide L-Glutamine-200 mm (100X), Liquid 20 ml D-Glucose (Dextrose) 1 kg Lipofectamine 2000 Transfection Reagent 0,75 ml HEPES (Ultra Pure) 1 kg Lipofectamine 2000 Transfection Reagent 1,5 ml IPTG 1 gr Invitrogen Lipofectamine 2000 Transfection Reagent 15 ml L-Glutamine, powder 1 kg Lipofectamine LTX & Plus Reagent 1 ml UltraPure 10 mg ml Ethidium Bromide 10 ml Lipofectamine Transfection Reagent 1 ml UltraPure Agarose 100 gr Lipofectamine Transfection Reagent 4 ml UltraPure Agarose Gibco Medium UltraPure Agarose gr Medium 199, HEPES UltraPure Buffer-Saturated Phenol MEM UltraPure Disodium Salt, Dihydrate (EDTA) MEM Amino Acids Solution (50X), Liquid UltraPure Dithiothreitol 5 gr Gibco MEM Non-Essential Amino Acids Solution 10 mm (100X), Liquid UltraPure DNase/RNase-Free Distilled Water MEM Sodium Pyruvate Solution 100 mm (100X), Liquid UltraPure Glycerol MEM, Powder UltraPure Glycine MEM, Powder, NEAA 1 x 50 L UltraPure Glycogen 100 ul Gibco MEM, Powder, NEAA 1 x 10 L UltraPure N,N'-Methylenebisacrylamide 100 gr Neurobasal Medium (1X), Liquid UltraPure Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) OPTI MEM I, con Glutamax UltraPure Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) Opti-MEM Reduced Serum Medium UltraPure Salmon Sperm DNA Solution 5 x 1ml Gibco PBS, 10X, ph UltraPure Sodium Dodecyl Sulfate (SDS) PBS, 1X, ph UltraPure TEMED 30 ml PBS, 1X, ph 7.4 6x1000 ml UltraPure Tris 1 kg Penicillin-Streptomycin, Liquid UltraPure Urea Gibco Penicillin-Streptomycin, Liquid 20 ml Life Technologies Water, Distilled 1L Life Technologies Phytohemagglutinin (M Form) (PHA), Liquid 10 ml X Gal 100 mg Proteinase K 100 mg Qualified Fetal Bovine Serum (FBS) - Origen Nueva Zelanda Gibco Qualified Fetal Bovine Serum (FBS) - Origen Nueva Zelanda RPMI, Glutamine RPMI, HEPES, Glutamine RPMI, HEPES, Glutamine 4

6 Biostock CULTIVO CELULAR RPMI, no Glutamine RPMI, no Phenol Red RPMI, Powder RPMI, Powder, HEPES TrypLE Express (1X), no Phenol Red Trypsin (1:250) Powder 100 gr % Trypsin-EDTA (1X), Phenol Red % Trypsin (1X), Phenol Red % Trypsin (1X), Phenol Red % Trypsin-EDTA (1X), Phenol Red % Trypsin-EDTA (1X), Phenol Red % Trypsin-EDTA (10X), no Phenol Red % Trypsin (10X), no Phenol Red 5

7 PCR, RT-PCR Y qpcr mm dctp 250 ul mm datp 250 ul mm dgtp 250 ul mm dntp Set 4 x 25 umol mm dttp 250 ul X BlueJuice Gel Loading Buffer 3 x 1 ml AmpliTaq Gold DNA Polymerase, 250 units with Gold Buffer and MgCl2 sol. 250 unid N AmpliTaq Gold DNA Polymerase, with Buffer II and MgCl2 sol. 250 U DNase I U DNase I, Amplification Grade 100 U M-mlV Reverse Transcriptase U M-mlV Reverse Transcriptase U Oligo(dT)12 18 Primer 25 ug Pfx50 DNA Polymerase Platinum Pfx DNA Polymerase Platinum Quantitative PCR SuperMix-UDG Platinum SYBR Green qpcr SuperMix-UDG Platinum Taq DNA Polymerase - Origen Brasil 500 U Platinum Taq DNA Polymerase - Origen Brasil 5000 U Platinum Taq DNA Polymerase - Origen Brasil U Platinum Taq DNA Polymerase High Fidelity Power SYBR Green PCR Master Mix, 1-Pack (1 x 5 ml) 200 reacc Random Primers (~297 ug/100ul) 100 ul RNaseOUT Recombinant Ribonuclease Inhibitor 125 reacc ROX Reference Dye 500 ul SuperScript II Reverse Transcriptase 10 reacc SuperScript II Reverse Transcriptase 50 reacc SuperScript III First-Strand Synthesis SuperMix 50 reacc SuperScript III First-Strand Synthesis System 50 reacc SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity SuperScript III One-Step RT-PCR System with Platinum Taq 6

8 Biostock 2013 PCR, RT-PCR Y qpcr SuperScript III Platinum SYBR Green One-Step qrt-pcr Kit SuperScript III Platinum One-Step qrt-pcr Kit SuperScript III Platinum One-Step qrt-pcr Kit 500 reacc SuperScript III Reverse Transcriptase 50 reacc SYBR Green PCR Master Mix, 1-Pack (1 x 5 ml) 200 reacc SYBR Select Master Mix, 1-Pack (1 x 5 ml) 500 reacc Taq DNA Polymerase, Native 500 U Taq DNA Polymerase, Recombinant 500 U Taq DNA Polymerase, Recombinant - Origen Brasil 500 U Taq DNA Polymerase, Recombinant - Origen Brasil U TaqMan TaqMan Gene Expression Master Mix, 1-Pack (1 x 5 ml) 200 reacc TaqMan TaqMan Universal Master Mix II, no UNG 1-Pack (1 x 5 ml) 200 reacc TaqMan TaqMan Universal PCR Master Mix, 1-Pack (1 x 5 ml) 200 reacc TaqMan TaqMan Universal PCR Master Mix, 5 pack (5 x 5 ml) 1000 reacc 7

9 REACTIVOS Y CONSUMIBLES PARA SECUENCIADORES AUTOMÁTICOS POLIMEROS POP-6 Polymer for the 310 Genetic Analyzer POP-4 Polymer for the 310 Genetic Analyzer POP-4 Polymer for 3100/3100-Avant; Genetic Analyzers POP-6 Polymer for 3100/3100-Avant Genetic Analyzers POP-4 Polymer for 3130/3130xl Genetic Analyzers, 7000 µl POP-7 Polymer for 3130/3130xl Genetic Analyzers, 7000 µl POP-7 Polymer for 3500/3500xL Genetic Analyzers POP-4 Polymer for 3500/3500xL Genetic Analyzers 3 ml 5 ml 7000 µl 7000 µl 7000 µl 7000 µl 384 reacc 384 reacc ARREGLOS CAPILARES cm 310 Capillary Array 5 U cm 3100 Capillary Array cm 3100 Capillary Array cm 3100-Avant Capillary Array cm 3500 Capillary Array cm 3500 Capillary Array MATRICES y ESTÁNDARES GeneScan 500 ROX Size Standard 800 reacc GeneScan 500 LIZ Size Standard 800 reacc /31xx Genetic Analyzer Sequencing Standards, BigDye Terminator v /31xx Genetic Analyzer Sequencing Standards, BigDye Terminator v /3500xL Sequencing Install Standard, BigDye Terminator v3.1 4 tubos 8

10 Biostock REACTIVOS Y CONSUMIBLES PARA SECUENCIADORES AUTOMÁTICOS REACTIVOS BigDye Terminator v1.1 Cycle Sequencing Kit BigDye Terminator v3.1 Cycle Sequencing Kit 310 and 31xx Running Buffer, 10X Hi-Di Formamide, 25 ml BigDye Terminator v1.1 & v3.1 5X Sequencing Buffer Conditioning Reagent, 3500 Series Anode Buffer Container (ABC) 3500 Series Cathode Buffer Container (CBC) 3500 Series BigDye XTerminator Purification Kit - 2 ml (~ µl Reactions) Streptavidin 25 ml 25 ml 1 ml 4 U 4 U 1 mg PLACAS, TUBOS, STRIP DE TUBOS, TAPAS Y FILM N MicroAmp Optical 96-Well Reaction Plate 10 placas MicroAmp 48-Well Optical Adhesive Film 25 films MicroAmp 96- & 384-Well Optical Adhesive Film 25 films MicroAmp 96- & 384-Well Optical Adhesive Film 100 films MicroAmp Fast 8-Tube Strip, 0.1 ml 125 tiras MicroAmp Fast 96-Well Reaction Plate, 0.1 ml 10 placas MicroAmp Fast Optical 48-Well Reaction Plate 0,1 ml 20 placas MicroAmp Fast Optical 96-Well Reaction Plate with Barcode, 0.1 ml 20 placas MicroAmp Optical 8-Cap Strip 300 tiras MicroAmp Optical 8-Tube Strip, 0.2 ml (1000 tubos x 125 strips) 125 tiras MicroAmp Optical 96-Well Reaction Plate with Barcode 0,2 ml 20 placas N MicroAmp Reaction Tube with Cap, 0.2 ml 1000 tubos 9

11 PURIFICACIÓN, CUANTIFICACIÓN Y TINCIÓN DE ÁCIDOS NUCLEICOS DNAzol Reagent AM1556 mirvana PARIS Kit 40 purif. K PureLink HiPure Plasmid Midiprep Kit 25 preps K PureLink Quick Gel Extraction Kit 50 preps A PureLink RNA Mini Kit 50 preps PureLink Viral RNA/DNA Mini Kit 50 preps Q32856 Qubit Assay Tubes - set of tubos Q32850 Qubit dsdna BR Assay Kit, 100 assays ng* 100 ensayos Q32851 Qubit dsdna HS Assay Kit, 100 assays ng* 100 ensayos Q33211 Qubit Protein Assay Kit, 100 assays µg* Q32852 Qubit RNA Assay Kit, 100 assays ng* 100 ensayos AM7020 RNAlater AM1912 RNAqueous Kit 50 preps G6601 Safe Imager 2.0 Amber Filter Replacement G6600 Safe Imager 2.0 Blue-Light Transilluminator S11494 SYBR Gold Nucleic Acid Gel Stain 10,000X concentrate in DMSO* 500 ul S7563 SYBR Green I Nucleic Acid Gel Stain - 10,000X concentrate in DMSO, 500 µl 500 ul S33102 SYBR Safe DNA Gel Stain 400 ul TRIzol LS Reagent 200 ml TRIzol LS Reagent TRIzol Reagent 200 ml TRIzol Reagent * for use with the Qubit 2.0 Fluorometer 10

12 Biostock ULTRAPUROS UltraPure Acrylamide Gibco D-Glucose (Dextrose) 1 kg HEPES (Ultra Pure) 1 kg IPTG 1 gr L-Glutamine, powder 1 kg UltraPure 10 mg ml Ethidium Bromide 10 ml UltraPure Agarose 100 gr UltraPure Agarose UltraPure Agarose gr UltraPure Buffer-Saturated Phenol UltraPure Disodium Salt, Dihydrate (EDTA) UltraPure Dithiothreitol 5 gr UltraPure DNase/RNase-Free Distilled Water UltraPure Glycerol UltraPure Glycine UltraPure Glycogen 100 ul UltraPure N,N'-Methylenebisacrylamide 100 gr UltraPure Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) UltraPure Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) UltraPure Salmon Sperm DNA Solution 5 x 1ml UltraPure Sodium Dodecyl Sulfate (SDS) UltraPure TEMED 30 ml UltraPure Tris 1 kg UltraPure Urea Life Technologies Water, Distilled 1 L X Gal 100 mg 11

13 MARCADORES Kb Plus DNA Ladder 250 ug bp DNA Ladder 50 ug bp DNA Ladder 250 ug bp DNA Ladder 50 ug Novex BenchMark Pre-Stained Protein Ladder 2 x 250 ul Low DNA Mass Ladder 50 applic CLONADO K TOPO TA Cloning Kit for subcloning with TOP10 E. coli 20 reacc EcoR I 5000 U Gateway LR Clonase Enzyme mix 80 ul Gateway LR Clonase II Enzyme mix 20 reacc Mse I 100 U TOPO TA Cloning Kit for Subcloning without Competent Cells 20 reacc Xho I (PaeR7I) 2500 U ENZIMAS MODIFICADORAS Ribonuclease H (E. coli) 30 U T4 DNA Ligase 100 U REACTIVOS PARA EXPERIMENTOS INMUNOLÓGICOS TMB Solution (ELISA) 12

14 Biostock 2013 INSTRUMENTOS Applied Biosystems 2720 Thermal Cycler 1 instrumento C10228 Countess Cell Counting Chamber Slides, 50 Slides (100 Counts) 1 caja Q32866 Qubit 2.0 Fluorometer 1 instrumento Q32871 Qubit 2.0 Quantitation Starter Kit 1 kit G6600 Safe Imager 2.0 Blue-Light Transilluminator 1 instrumento StepOne Real-Time PCR System 1 instrumento StepOnePlus Real-Time PCR System 1 instrumento Veriti 96-Well Thermal Cycler 1 instrumento Contacte a su asesor de ventas para más información 13

15 14

16 Contactos Atención al Cliente T (011) /2800 F LIFE (5433) Asesores comerciales Representante de Consumibles Gabriel Barrio gabriel.barrio@lifetech.com Lic. César Villa cesar.villa@lifetech.com Tec. Noelia Balbi noelia.balbi@lifetech.com Instrumentos Lic. Eduardo Holmes-Brown eduardo.holmes-brown@lifetech.com Bioproducción Lic. Matías Acuña matias.acuna@lifetech.com Inside Sales Daniela Violante daniela.violante@lifetech.com Soporte técnico/aplicaciones Lic. Andrea Merás Dra. Laura Otero Lic. Fernando Pérez Rojo tech.support.arg@lifetech.com lifetechnologies.com PRODUCTOS PARA USO EXCLUSIVO EN INVESTIGACIÓN. NO RECOMENDADO PARA TERAPIA O DIAGNÓSTICO EN ANIMALES O HUMANOS Life Technologies Company. Todos los derechos reservados. Las marcas comerciales mencionadas en este documento son de propiedad de Life Technologies Company o de sus respectivos propietarios. TaqMan es marca registrada de Roche Molecular Systems, Inc., utilizada bajo permiso y licencia.